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Protein

Eukaryotic translation initiation factor 3 subunit E

Gene

eIF3e

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation (PubMed:18493598). In addition to its role in the eIF-3 complex, also functions in protein ubiquitination and degradation (PubMed:18493598, PubMed:28505193). During mitosis required for regulating mitotic microtubule growth and kinetochore formation, and consequently is required for satisfying the spindle assembly checkpoint (SAC) during metaphase to prevent delays in mitotic progression (PubMed:28505193). This is likely by promoting the ubiquitination and degradation of Klp67A, a kinesin-like protein that suppresses microtubule polymerization at plus ends (PubMed:28505193). Acts in the COP9 signalosome (CSN) mediated regulation of cullin neddylation by promoting Cul1 and Cul3 neddylation and negatively regulating the CSN complex subunit CSN5 (PubMed:18493598).UniRule annotation2 Publications

GO - Molecular functioni

  • translation initiation factor activity Source: FlyBase

GO - Biological processi

  • translational initiation Source: FlyBase

Keywordsi

Molecular functionInitiation factor
Biological processProtein biosynthesis

Enzyme and pathway databases

ReactomeiR-DME-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-DME-72649. Translation initiation complex formation.
R-DME-72689. Formation of a pool of free 40S subunits.
R-DME-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-DME-72702. Ribosomal scanning and start codon recognition.

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 3 subunit EUniRule annotation
Short name:
eIF3eUniRule annotation
Alternative name(s):
Eukaryotic translation initiation factor 3 subunit 6UniRule annotation
Gene namesi
Name:eIF3eUniRule annotation
Synonyms:eIF-3p481 Publication, eIF3-S6UniRule annotation, Int61 PublicationUniRule annotation
ORF Names:CG9677Imported
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0025582. eIF3e.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Microsome

Pathology & Biotechi

Disruption phenotypei

Homozygous lethal at the first instar larval stage. Larvae are smaller than wild-type and die 3-4 days after hatching.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001235171 – 435Eukaryotic translation initiation factor 3 subunit EAdd BLAST435

Proteomic databases

PaxDbiO77410.
PRIDEiO77410.

Expressioni

Tissue specificityi

Expression levels in females and males are relatively similar 10 days after oviposition, however by day 15 expression is higher in gravid females than in males (at protein level).1 Publication

Developmental stagei

Expressed throughout development and in adults (at protein level). Expression peaks in early embryos (2 h after oviposition), then steadily decreases over the embryonic and larval stages (at protein level). Expression increases again 5 days after oviposition and remains stable until day 10 (at protein level).1 Publication

Gene expression databases

BgeeiFBgn0025582.
ExpressionAtlasiO77410. differential.
GenevisibleiO77410. DM.

Interactioni

Subunit structurei

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex. The eIF-3 complex interacts with pix. Interacts with mxt (PubMed:23716590).UniRule annotation1 Publication

Protein-protein interaction databases

BioGridi65176. 17 interactors.
DIPiDIP-19544N.
IntActiO77410. 3 interactors.
STRINGi7227.FBpp0075104.

Structurei

3D structure databases

ProteinModelPortaliO77410.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini219 – 392PCIPROSITE-ProRule annotationAdd BLAST174

Sequence similaritiesi

Belongs to the eIF-3 subunit E family.UniRule annotation

Phylogenomic databases

eggNOGiKOG2758. Eukaryota.
ENOG410XQK5. LUCA.
GeneTreeiENSGT00390000002661.
InParanoidiO77410.
KOiK03250.
OMAiGFFRTQV.
OrthoDBiEOG091G075K.
PhylomeDBiO77410.

Family and domain databases

HAMAPiMF_03004. eIF3e. 1 hit.
InterProiView protein in InterPro
IPR016650. eIF3e.
IPR019010. eIF3e_N.
IPR000717. PCI_dom.
IPR036390. WH_DNA-bd_sf.
PANTHERiPTHR10317. PTHR10317. 1 hit.
PfamiView protein in Pfam
PF09440. eIF3_N. 1 hit.
PF01399. PCI. 1 hit.
PIRSFiPIRSF016255. eIF3e_su6. 1 hit.
SMARTiView protein in SMART
SM01186. eIF3_N. 1 hit.
SM00088. PINT. 1 hit.
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiView protein in PROSITE
PS50250. PCI. 1 hit.

Sequencei

Sequence statusi: Complete.

O77410-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANFDLTRIN CQFLDRHLTF PLLEFLCGKE IYNQQELLEY ILETVNKTNM
60 70 80 90 100
IDYTMDTRKR LNLSQEMPEE LVQRKAEVLA TLKQLQNEVA PIMKATDILK
110 120 130 140 150
NGESMKDSKT FVNALQKDYN FKVEHLESAY KLAKYLYECG NYQESTSYLY
160 170 180 190 200
FCLIVMSPND KNYLNVLWGK LAAEILTLNW NTALEDLTRL RDYIDNANFS
210 220 230 240 250
TIQALQQRTW LIHWSVLVFF NHPKGRDLII EMFLYKPLYL NAIQTMCPHI
260 270 280 290 300
MRYLATAVVI NRTRRNALKD LIKVIQQESY TYRDPITEFL ECLYVNFDFE
310 320 330 340 350
GARLKLHECQ TVILNDFFIV ACLNEFVEDA RLMIFETFCR IHQCITISML
360 370 380 390 400
ADKLNMKPNE AECWIVNLIR NARLNAKIDS KLGHVVMGTQ PLSPYQQLVE
410 420 430
KIDSLSMRSE HLAGLIERKS KQKQNQESAD SWKYY
Length:435
Mass (Da):51,162
Last modified:November 1, 1998 - v1
Checksum:i6F79D32FBA3EA711
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U89162 mRNA. Translation: AAC62307.1.
AE014296 Genomic DNA. Translation: AAF49412.1.
AF132551 mRNA. Translation: AAD27850.1.
RefSeqiNP_477385.1. NM_058037.5.
UniGeneiDm.3105.

Genome annotation databases

EnsemblMetazoaiFBtr0075345; FBpp0075104; FBgn0025582.
GeneIDi39877.
KEGGidme:Dmel_CG9677.

Similar proteinsi

Entry informationi

Entry nameiEIF3E_DROME
AccessioniPrimary (citable) accession number: O77410
Secondary accession number(s): Q9VVA2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1998
Last modified: February 28, 2018
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome