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Protein

RNA 3'-terminal phosphate cyclase

Gene

CG4061

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing (By similarity).By similarity

Catalytic activityi

ATP + (RNA)-3'-(3'-phospho-ribonucleoside) = AMP + diphosphate + (RNA)-3'-(2',3'-cyclophospho)-ribonucleoside.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei105ATPBy similarity1
Active sitei321Tele-AMP-histidine intermediateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi295 – 299ATPBy similarity5

GO - Molecular functioni

GO - Biological processi

  • negative regulation of axon regeneration Source: FlyBase
  • RNA processing Source: InterPro

Keywordsi

Molecular functionLigase
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
RNA 3'-terminal phosphate cyclase (EC:6.5.1.4)
Short name:
RNA cyclase
Short name:
RNA-3'-phosphate cyclase
Gene namesi
ORF Names:CG4061
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0025630 CG4061

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001564121 – 361RNA 3'-terminal phosphate cyclaseAdd BLAST361

Proteomic databases

PaxDbiO77264
PRIDEiO77264

Expressioni

Gene expression databases

BgeeiFBgn0025630
ExpressionAtlasiO77264 baseline and differential
GenevisibleiO77264 DM

Interactioni

Protein-protein interaction databases

BioGridi57719, 6 interactors
DIPiDIP-23093N
STRINGi7227.FBpp0070316

Structurei

3D structure databases

ProteinModelPortaliO77264
SMRiO77264
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3980 Eukaryota
COG0430 LUCA
InParanoidiO77264
KOiK01974
OMAiANRPKPG
OrthoDBiEOG091G0JQJ
PhylomeDBiO77264

Family and domain databases

Gene3Di3.30.360.20, 1 hit
3.65.10.20, 2 hits
HAMAPiMF_00200 RTC, 1 hit
InterProiView protein in InterPro
IPR013791 RNA3'-term_phos_cycl_insert
IPR023797 RNA3'_phos_cyclase_dom
IPR037136 RNA3'_phos_cyclase_dom_sf
IPR000228 RNA3'_term_phos_cyc
IPR017770 RNA3'_term_phos_cyc_type_1
IPR013792 RNA3'P_cycl/enolpyr_Trfase_a/b
IPR036553 RPTC_insert
PANTHERiPTHR11096 PTHR11096, 1 hit
PTHR11096:SF0 PTHR11096:SF0, 1 hit
PfamiView protein in Pfam
PF01137 RTC, 1 hit
PF05189 RTC_insert, 1 hit
PIRSFiPIRSF005378 RNA3'_term_phos_cycl_euk, 1 hit
SUPFAMiSSF52913 SSF52913, 1 hit
SSF55205 SSF55205, 2 hits
TIGRFAMsiTIGR03399 RNA_3prim_cycl, 1 hit

Sequencei

Sequence statusi: Complete.

O77264-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDAEKMLEID GSYLEGGGQA LRNALSLSCI LGKPVRVVKI RASRPSPGLS
60 70 80 90 100
HQHLHGLNLL RDITNADVVG NYLLSSTVEF TPRTILDNTY RVETHTAASI
110 120 130 140 150
TLIYQMALPV LLFAGRPSRL IVSGGTNVDF APPVEYMQEV LLPNLKHFGV
160 170 180 190 200
SFDLKVQRYG FYPRGQGRCQ LDVQPVTKLN SGKLVAFGRI KSVSGVAYCA
210 220 230 240 250
GRLPVNIAID MQQTAQREIH RLWPSQQCSI EPIKHSRQKA FHNGAGILMT
260 270 280 290 300
VNTTSDVVLG ASALGKKRID GHVLGSEASC QLGDYMRKQV CVDDYMQDQL
310 320 330 340 350
IIYMALAVGR STMRTGKLTN HTRTAINVAE QMTGVKFDVA MEPGGQMLVS
360
CKGLGHVNKL I
Length:361
Mass (Da):39,503
Last modified:November 1, 1998 - v1
Checksum:iCDB65A453BB8CF39
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014298 Genomic DNA Translation: AAF45719.1
AL031765 Genomic DNA Translation: CAA21129.1
AY052112 mRNA Translation: AAK93536.1
PIRiT13745
RefSeqiNP_001284801.1, NM_001297872.1
NP_569977.1, NM_130621.3
UniGeneiDm.33463

Genome annotation databases

EnsemblMetazoaiFBtr0070330; FBpp0070316; FBgn0025630
FBtr0344156; FBpp0310569; FBgn0025630
GeneIDi31175
KEGGidme:Dmel_CG4061
UCSCiCG4061-RA d. melanogaster

Similar proteinsi

Entry informationi

Entry nameiRTCA_DROME
AccessioniPrimary (citable) accession number: O77264
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1998
Last modified: May 23, 2018
This is version 149 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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