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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

CG4045

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N7-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei79S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei157S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Active sitei160UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, RNA-binding, Transferase, tRNA-binding
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
ORF Names:CG4045
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0025629 CG4045

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001714341 – 256tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST256

Proteomic databases

PaxDbiO77263
PRIDEiO77263

Expressioni

Gene expression databases

BgeeiFBgn0025629
GenevisibleiO77263 DM

Interactioni

Protein-protein interaction databases

BioGridi57720, 2 interactors
DIPiDIP-18791N
IntActiO77263, 2 interactors
STRINGi7227.FBpp0070317

Structurei

3D structure databases

ProteinModelPortaliO77263
SMRiO77263
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni102 – 103S-adenosyl-L-methionine bindingUniRule annotation2
Regioni137 – 138S-adenosyl-L-methionine bindingUniRule annotation2
Regioni235 – 237S-adenosyl-L-methionine bindingUniRule annotation3

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiKOG3115 Eukaryota
COG0220 LUCA
GeneTreeiENSGT00390000017840
InParanoidiO77263
KOiK03439
OMAiIGMEIRV
OrthoDBiEOG091G0IQ7
PhylomeDBiO77263

Family and domain databases

HAMAPiMF_03055 tRNA_methyltr_TrmB_euk, 1 hit
InterProiView protein in InterPro
IPR029063 SAM-dependent_MTases
IPR025763 Trm8_euk
IPR003358 tRNA_(Gua-N-7)_MeTrfase_Trmb
PANTHERiPTHR23417 PTHR23417, 1 hit
PTHR23417:SF16 PTHR23417:SF16, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00091 TIGR00091, 1 hit
PROSITEiView protein in PROSITE
PS51625 SAM_MT_TRMB, 1 hit

Sequencei

Sequence statusi: Complete.

O77263-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVATGGQAQD QGQNQEPDVL NPTSAVTGLP QKRYYRQRAH SNPIADHSFD
60 70 80 90 100
YPARPEDVDW RSMYPGIQQG QQVSFADIGC GYGGFLVTLG EMFPEKLSIG
110 120 130 140 150
MEIRVKVSDY VVDRIAALRR RCADTGAYQN IACLRTNAMK YLPNYFVKGQ
160 170 180 190 200
LEKMFFLYPD PHFKRAKHKW RIINQALLSE YAYILRKGGL LYTMTDVEDL
210 220 230 240 250
HKWIVTHMEE HPLYERLTEE EANADPITPK LYQSSEEGAK VVRNKGDHFL

AIFRRL
Length:256
Mass (Da):29,425
Last modified:November 1, 1998 - v1
Checksum:i9E0F0B8AC97FC00E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014298 Genomic DNA Translation: AAF45720.2
AL031765 Genomic DNA Translation: CAA21130.1
AY094742 mRNA Translation: AAM11095.1
PIRiT13746
RefSeqiNP_569978.1, NM_130622.3
UniGeneiDm.26191

Genome annotation databases

EnsemblMetazoaiFBtr0070331; FBpp0070317; FBgn0025629
GeneIDi31176
KEGGidme:Dmel_CG4045
UCSCiCG4045-RA d. melanogaster

Similar proteinsi

Entry informationi

Entry nameiTRMB_DROME
AccessioniPrimary (citable) accession number: O77263
Secondary accession number(s): Q9W527
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: November 1, 1998
Last modified: May 23, 2018
This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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