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O77229 (CATA_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Catalase-A

EC=1.11.1.6
Gene names
Name:catA
Synonyms:cat
ORF Names:DDB_G0274595
OrganismDictyostelium discoideum (Slime mold)
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length496 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Ref.4

Catalytic activity

2 H2O2 = O2 + 2 H2O.

Cofactor

Heme group By similarity.

Subcellular location

Peroxisome By similarity.

Developmental stage

Expressed throughout growth and development. Exclusively localized in the prestalk cells. Ref.1

Disruption phenotype

Cells are resistant to methanol, acriflavine and thiabendazole. Ref.4

Sequence similarities

Belongs to the catalase family.

Ontologies

Keywords
   Biological processHydrogen peroxide
   Cellular componentPeroxisome
   LigandHeme
Iron
Metal-binding
   Molecular functionOxidoreductase
Peroxidase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processhydrogen peroxide catabolic process

Inferred from direct assay Ref.1. Source: dictyBase

response to methanol

Inferred from mutant phenotype Ref.4. Source: dictyBase

   Cellular componentperoxisome

Inferred from direct assay Ref.1. Source: dictyBase

phagocytic vesicle

Inferred from direct assay. Source: dictyBase

   Molecular functioncatalase activity

Inferred from direct assay Ref.1. Source: dictyBase

heme binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 496496Catalase-A
PRO_0000084911

Regions

Motif494 – 4963Microbody targeting signal Potential

Sites

Active site541 By similarity
Active site1281 By similarity
Metal binding3381Iron (heme axial ligand) By similarity

Experimental info

Sequence conflict671P → T in AAC36743. Ref.1

Sequences

Sequence LengthMass (Da)Tools
O77229 [UniParc].

Last modified December 4, 2007. Version 2.
Checksum: 254CA4D38E698C9F

FASTA49655,680
        10         20         30         40         50         60 
MSAPVLTTSS GSPIDNNLNS MTAGVNGPIL IQDFTLIDKL AHFDRERIPE RVVHAKGAGA 

        70         80         90        100        110        120 
HGYFEVPSSD VPKWCKAKFL NKVGKRTPIF TRFSTVGGEK GSSDSERDPR GFAVKFYTEE 

       130        140        150        160        170        180 
GNFDMVGNNT PVFFIRDPSK FPDFIHTQKR NPQTNCKDPN MFWDFLGQTP ESTHQVSILF 

       190        200        210        220        230        240 
SDRGTPKSYR HMHGFSSHTL KFVNAQGKPY WVKLHFTSET GIQNYTAEEA AKMSMNDPDS 

       250        260        270        280        290        300 
ATRDLFETIA KGGEPAWKVS IQLMEFEDAL KYRFNPFDVT KIWSHKDYPL IQIGRMVLNR 

       310        320        330        340        350        360 
NPENYFAEVE QAAFSPSHMV PGIEPSPDKM LQGRLFSYPD THRHRLGVNY QQIPVNCPFA 

       370        380        390        400        410        420 
VKGGVKNYQR DGFMAVNGNG GKGPNYQPNS FGGPEPHPEF AQHKFDVSGF AARQPYNHPN 

       430        440        450        460        470        480 
DDFVQPGDLY RLMSEDAKSR FVSNLVGHMS GVTIKEIQVR AVSNFYKADK DLGARLCKGL 

       490 
GIDVNDVIKF AARSNL 

« Hide

References

« Hide 'large scale' references
[1]"Differential developmental expression and cell type specificity of Dictyostelium catalases and their response to oxidative stress and UV-light."
Garcia M.X.U., Foote C., van Es S., Devreotes P.N., Alexander S., Alexander H.
Biochim. Biophys. Acta 1492:295-310(2000) [PubMed: 11004503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
Strain: AX3.
[2]"Sequence and analysis of chromosome 2 of Dictyostelium discoideum."
Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A.
Nature 418:79-85(2002) [PubMed: 12097910] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[3]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed: 15875012] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[4]"Methanol and acriflavine resistance in Dictyostelium are caused by loss of catalase."
Garcia M.X.U., Roberts C., Alexander H., Stewart A.M., Harwood A., Alexander S., Insall R.H.
Microbiology 148:333-340(2002) [PubMed: 11782526] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF090443 mRNA. Translation: AAC36743.1.
AAFI02000012 Genomic DNA. Translation: EAL70190.1.
RefSeqXP_643894.1. XM_638802.1.

3D structure databases

ProteinModelPortalO77229.
SMRO77229. Positions 2-481.
ModBaseSearch...

Protein-protein interaction databases

STRINGO77229.

Protein family/group databases

PeroxiBase4096. DdKat01.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0185123; DDB0185123; DDB_G0274595.
GeneID8619320.
GenomeReviewsGene locus catA in contig CM000151_GR.
KEGGddi:DDB_G0274595.

Organism-specific databases

dictyBaseDDB_G0274595. catA.

Phylogenomic databases

eggNOGKOG0047.
GeneTreeEPrGT00050000005287.
HOGENOMHBG339355.
OMAVEPANFA.
PhylomeDBO77229.
ProtClustDBCLSZ2430996.

Family and domain databases

InterProIPR018028. Catalase.
IPR020835. Catalase-like_dom.
IPR024708. Catalase_AS.
IPR024711. Catalase_clade1/3.
IPR011614. Catalase_core.
IPR002226. Catalase_haem_BS.
IPR010582. Catalase_immune_responsive.
[Graphical view]
Gene3DG3DSA:2.40.180.10. Catalase_N. 1 hit.
KOK03781.
PANTHERPTHR11465. Catalase. 1 hit.
PfamPF00199. Catalase. 1 hit.
PF06628. Catalase-rel. 1 hit.
[Graphical view]
PIRSFPIRSF038928. Catalase_clade1-3. 1 hit.
PRINTSPR00067. CATALASE.
SMARTSM01060. Catalase. 1 hit.
[Graphical view]
SUPFAMSSF56634. Catalase_N. 1 hit.
PROSITEPS00437. CATALASE_1. 1 hit.
PS00438. CATALASE_2. 1 hit.
PS51402. CATALASE_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCATA_DICDI
AccessionPrimary (citable) accession number: O77229
Secondary accession number(s): Q556B6, Q86A71
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 4, 2007
Last modified: January 25, 2012
This is version 71 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase

SIMILARITY comments

Index of protein domains and families