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Protein

Beta-insect excitatory toxin BmK IT-AP

Gene

IT-AP

Organism
Mesobuthus martensii (Manchurian scorpion) (Buthus martensii)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Excitatory insect beta-toxins induce a spastic paralysis. They bind voltage-independently at site-4 of sodium channels (Nav) and shift the voltage of activation toward more negative potentials thereby affecting sodium channel activation and promoting spontaneous and repetitive firing. This insect toxin is also active on mice, showing an analgesic effect.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ion channel impairing toxin, Neurotoxin, Toxin, Voltage-gated sodium channel impairing toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-insect excitatory toxin BmK IT-AP
Alternative name(s):
Bm33-I
BmKITAP
Insect toxin analgesic peptide
Gene namesi
Name:IT-AP
OrganismiMesobuthus martensii (Manchurian scorpion) (Buthus martensii)
Taxonomic identifieri34649 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaScorpionesButhidaButhoideaButhidaeMesobuthus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Toxic dosei

PD50 is 0.49 ng/mg in Gryllus bimaculatus crickets.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 18182 PublicationsAdd
BLAST
Chaini19 – 9072Beta-insect excitatory toxin BmK IT-APPRO_0000035211Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi34 ↔ 551 Publication
Disulfide bondi40 ↔ 601 Publication
Disulfide bondi44 ↔ 621 Publication
Disulfide bondi56 ↔ 821 Publication

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.

Structurei

Secondary structure

1
90
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi20 – 256Combined sources
Helixi37 – 4812Combined sources
Beta strandi51 – 566Combined sources
Beta strandi59 – 657Combined sources
Helixi76 – 838Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1T0ZX-ray2.60A/B19-90[»]
ProteinModelPortaliO77091.
SMRiO77091. Positions 19-90.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO77091.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.30.30.10. 1 hit.
InterProiIPR003614. Scorpion_toxin-like.
IPR002061. Scorpion_toxinL/defensin.
[Graphical view]
PfamiPF00537. Toxin_3. 1 hit.
[Graphical view]
SMARTiSM00505. Knot1. 1 hit.
[Graphical view]
SUPFAMiSSF57095. SSF57095. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O77091-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFFLIFLVI FPIMGVLGKK NGYAVDSSGK VAECLFNNYC NNECTKVYYA
60 70 80 90
DKGYCCLLKC YCFGLADDKP VLDIWDSTKN YCDVQIIDLS
Length:90
Mass (Da):10,225
Last modified:November 1, 1998 - v1
Checksum:iED240D0BCF637EA2
GO

Mass spectrometryi

Molecular mass is 8149.39 Da from positions 19 - 90. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF055672 Genomic DNA. Translation: AAC61256.1.
AF156171 mRNA. Translation: AAF29464.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF055672 Genomic DNA. Translation: AAC61256.1.
AF156171 mRNA. Translation: AAF29464.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1T0ZX-ray2.60A/B19-90[»]
ProteinModelPortaliO77091.
SMRiO77091. Positions 19-90.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiO77091.

Family and domain databases

Gene3Di3.30.30.10. 1 hit.
InterProiIPR003614. Scorpion_toxin-like.
IPR002061. Scorpion_toxinL/defensin.
[Graphical view]
PfamiPF00537. Toxin_3. 1 hit.
[Graphical view]
SMARTiSM00505. Knot1. 1 hit.
[Graphical view]
SUPFAMiSSF57095. SSF57095. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Molecular characterization of a new excitatory insect neurotoxin with an analgesic effect on mice from the scorpion Buthus martensi Karsch."
    Xiong Y.-M., Lan Z.-D., Wang M., Liu B., Liu X.-Q., Fei H., Xu L.-G., Xia Q.-C., Wang C.-G., Wang D.-C., Chi C.-W.
    Toxicon 37:1165-1180(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 19-90.
    Tissue: Venom and Venom gland.
  2. Li W., Zhu S.
    Submitted (JUN-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Venom gland.
  3. "Sequence and electrophysiological characterization of two insect-selective excitatory toxins from the venom of the Chinese scorpion Buthus martensi."
    Escoubas P., Stankiewicz M., Takaoka T., Pelhate M., Romi-Lebrun R., Wu F.Q., Nakajima T.
    FEBS Lett. 483:175-180(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 19-90, ELECTROPHYSIOLOGICAL CHARACTERIZATION, PARALYTIC DOSE, MASS SPECTROMETRY.
    Tissue: Venom.
  4. "Crystallization and preliminary X-ray analyses of insect neurotoxins with analgesic effect from the scorpion Buthus martensii Karsch."
    Guan R.J., Liu X.Q., Liu B., Wang M., Wang D.C.
    Acta Crystallogr. D 56:1012-1014(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF 19-90, DISULFIDE BONDS.

Entry informationi

Entry nameiSIXP_MESMA
AccessioniPrimary (citable) accession number: O77091
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: November 1, 1998
Last modified: October 14, 2015
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.