O77081 (TPSTA_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein-tyrosine sulfotransferase A
EC=2.8.2.20

Alternative name(s):
Tyrosylprotein sulfotransferase A
Short name=TPST-A

Gene names

Name:	tpst-1
ORF Names:	Y111B2A.15

Organism

Caenorhabditis elegans

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	380 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the O-sulfation of tyrosine residues within acidic motifs of polypeptides.
Catalytic activity	3'-phosphoadenylyl sulfate + protein tyrosine = adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein By similarity.
Sequence similarities	Belongs to the protein sulfotransferase family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	body morphogenesis Inferred from mutant phenotype. Source: WormBase collagen metabolic process Inferred from mutant phenotype. Source: WormBase cuticle development involved in collagen and cuticulin-based cuticle molting cycle Inferred from mutant phenotype. Source: WormBase growth Inferred from mutant phenotype. Source: WormBase hermaphrodite genitalia development Inferred from mutant phenotype. Source: WormBase locomotion Inferred from mutant phenotype. Source: WormBase negative regulation of multicellular organism growth Inferred from mutant phenotype. Source: WormBase nematode larval development Inferred from mutant phenotype. Source: WormBase peptidyl-tyrosine sulfation Inferred from direct assay Ref.1. Source: WormBase positive regulation of growth rate Inferred from mutant phenotype. Source: WormBase positive regulation of locomotion Inferred from mutant phenotype. Source: WormBase positive regulation of multicellular organism growth Inferred from mutant phenotype. Source: WormBase regulation of nematode larval development Inferred from mutant phenotype. Source: WormBase
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW trans-Golgi network Inferred from direct assay. Source: WormBase
Molecular function	protein-tyrosine sulfotransferase activity Inferred from direct assay Ref.1. Source: WormBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 380	380	Protein-tyrosine sulfotransferase A		PRO_0000189831
Regions
	Topological domain	1 – 6	6	Cytoplasmic Potential
	Transmembrane	7 – 27	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	28 – 380	353	Lumenal Potential
Amino acid modifications
	Glycosylation	66	1	N-linked (GlcNAc...) Ref.3

Sequences

	Sequence		Length	Mass (Da)
	O77081 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: FF709BF00F1EDC95	FASTA	380	43,314
	10 20 30 40 50 60 MRKNRELLLV LFLVVFILFY FITARTADDP YYSNHREKFN GAAADDGDES LPFHQLTSVR 70 80 90 100 110 120 SDDGYNRTSP FIFIGGVPRS GTTLMRAMLD AHPEVRCGEE TRVIPRILNL RSQWKKSEKE 130 140 150 160 170 180 WNRLQQAGVT GEVINNAISS FIMEIMVGHG DRAPRLCNKD PFTMKSAVYL KELFPNAKYL 190 200 210 220 230 240 LMIRDGRATV NSIISRKVTI TGFDLNDFRQ CMTKWNAAIQ IMVDQCESVG EKNCLKVYYE 250 260 270 280 290 300 QLVLHPEAQM RRITEFLDIP WDDKVLHHEQ LIGKDISLSN VERSSDQVVK PVNLDALIKW 310 320 330 340 350 360 VGTIPEDVVA DMDSVAPMLR RLGYDPNANP PNYGKPDELV AKKTEDVHKN GAEWYKKAVQ 370 380 VVNDPGRVDK PIVDNEVSKL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and expression of human and mouse tyrosylprotein sulfotransferase-2 and a tyrosylprotein sulfotransferase homologue in Caenorhabditis elegans." Ouyang Y.-B., Moore K.L. J. Biol. Chem. 273:24770-24774(1998) [PubMed: 9733778] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Bristol N2.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[3]	"Proteomics reveals N-linked glycoprotein diversity in Caenorhabditis elegans and suggests an atypical translocation mechanism for integral membrane proteins." Kaji H., Kamiie J., Kawakami H., Kido K., Yamauchi Y., Shinkawa T., Taoka M., Takahashi N., Isobe T. Mol. Cell. Proteomics 6:2100-2109(2007) [PubMed: 17761667] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-66, MASS SPECTROMETRY. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF049709 mRNA. Translation: AAC36062.1. AL132904 Genomic DNA. Translation: CAC35844.1.
PIR	T42755.
RefSeq	NP_499646.3. NM_067245.2.
UniGene	Cel.19647.
3D structure databases
ProteinModelPortal	O77081.
ModBase	Search...
Proteomic databases
PRIDE	O77081.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	Y111B2A.15; Y111B2A.15; Y111B2A.15.
GeneID	176684.
KEGG	cel:Y111B2A.15.
NMPDR	fig|6239.3.peg.11902.
Organism-specific databases
CTD	176684.
WormBase	Y111B2A.15; CE26632; WBGene00013737; tpst-1.
Phylogenomic databases
eggNOG	meNOG05430.
GeneTree	EMGT00050000006091.
HOGENOM	HBG382344.
InParanoid	O77081.
OMA	VLECRAV.
PhylomeDB	O77081.
Gene expression databases
ArrayExpress	O77081.
Family and domain databases
InterPro	IPR000863. Sulfotransferase_dom. [Graphical view]
KO	K01021.
Pfam	PF00685. Sulfotransfer_1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	893596.

Entry information

Entry name

TPSTA_CAEEL

Accession

Primary (citable) accession number: O77081
Secondary accession number(s): Q9NEW9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1998
Last modified:	November 16, 2011
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project