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Protein

Amino acid transporter

Gene

Eaat1

Organism
Drosophila melanogaster (Fruit fly)
Status
Unreviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  • glutamate:sodium symporter activity Source: FlyBase
  • L-aspartate transmembrane transporter activity Source: FlyBase
  • L-glutamate transmembrane transporter activity Source: FlyBase
  • sodium:dicarboxylate symporter activity Source: InterPro

GO - Biological processi

  • aspartate transport Source: FlyBase
  • determination of adult lifespan Source: FlyBase
  • larval locomotory behavior Source: FlyBase
  • L-aspartate transmembrane transport Source: GOC
  • L-glutamate transmembrane transport Source: GOC
  • L-glutamate transport Source: FlyBase
  • regulation of excitatory postsynaptic membrane potential involved in skeletal muscle contraction Source: FlyBase
  • synaptic transmission Source: FlyBase
Complete GO annotation...

Keywords - Biological processi

SymportUniRule annotation, Transport

Enzyme and pathway databases

ReactomeiR-DME-352230. Amino acid transport across the plasma membrane.

Names & Taxonomyi

Protein namesi
Recommended name:
Amino acid transporterUniRule annotation
Gene namesi
Name:Eaat1Imported
Synonyms:Dglt-1Imported
ORF Names:CG3747Imported, Dmel_CG3747Imported
OrganismiDrosophila melanogaster (Fruit fly)Imported
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0026439. Eaat1.

Subcellular locationi

  • Membrane UniRule annotation; Multi-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei20 – 3819HelicalUniRule annotationAdd
BLAST
Transmembranei59 – 8325HelicalUniRule annotationAdd
BLAST
Transmembranei95 – 11723HelicalUniRule annotationAdd
BLAST
Transmembranei234 – 25522HelicalUniRule annotationAdd
BLAST
Transmembranei267 – 29327HelicalUniRule annotationAdd
BLAST
Transmembranei372 – 39524HelicalUniRule annotationAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: FlyBase
  • neuron projection Source: FlyBase
  • plasma membrane Source: FlyBase
  • terminal bouton Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Interactioni

Protein-protein interaction databases

STRINGi7227.FBpp0079400.

Family & Domainsi

Sequence similaritiesi

Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. [View classification]UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helixUniRule annotation

Phylogenomic databases

eggNOGiKOG3787. Eukaryota.
COG1301. LUCA.
GeneTreeiENSGT00760000119117.
OMAiITFLRKM.
OrthoDBiEOG7RV9G2.

Family and domain databases

Gene3Di1.10.3860.10. 1 hit.
InterProiIPR001991. Na-dicarboxylate_symporter.
IPR018107. Na-dicarboxylate_symporter_CS.
[Graphical view]
PANTHERiPTHR11958. PTHR11958. 1 hit.
PfamiPF00375. SDF. 1 hit.
[Graphical view]
PRINTSiPR00173. EDTRNSPORT.
SUPFAMiSSF118215. SSF118215. 2 hits.
PROSITEiPS00714. NA_DICARBOXYL_SYMP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O77062-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTRPKQDGGK FKAFMQENVL TMATVIGVFV GGLIGFIIKN STGEWSKREI
60 70 80 90 100
MYISFPGEIF LRMLKCLIVP LLVSSITSAI GGLDLSMSSK IATRAITYYF
110 120 130 140 150
VTTISAVILG ICLVTTLRPG QGAKIVETQT ESIDKASKVL TPDTLMDLVR
160 170 180 190 200
NMFTDNIIQS TMFQHRTEIY ENTSISPAQP MENWEFKSAQ REGSNVLGLV
210 220 230 240 250
MFSVILGTTI GRMREKGQLL QDFFTTLSEA MMTITSWVIW ISPLGVAFLI
260 270 280 290 300
AAKIIEMESI AATIQSLGWY FITVMIGLFL HGFGTIAVIF FLGTRRLPYR
310 320 330 340 350
YIAKLSQVLA TAFGTGSSSA TMPLTIKCLD NMGIDPRVTR FVIPVGATIN
360 370 380 390 400
MDGTALYEAV AALFIAQYRE MSYSFGTIVA VSITATAASI GAAGIPQAGL
410 420 430 440 450
VTMVMVLDTV GLEPKDVSLI IAVDWLLDRF RTTINVMCDA LGTILVNHLS
460 470
KNDLASVDRL NAEPHELLEL GPNGHEMKE
Length:479
Mass (Da):52,359
Last modified:November 1, 1998 - v1
Checksum:i25B5772972750AB8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF001784 mRNA. Translation: AAD09142.1.
AF165999 mRNA. Translation: AAD47829.1.
AE014134 Genomic DNA. Translation: AAF52764.1.
AE014134 Genomic DNA. Translation: AAN10691.1.
AE014134 Genomic DNA. Translation: AAN10692.1.
BT044100 mRNA. Translation: ACH92165.1.
AE014134 Genomic DNA. Translation: ADV37001.1.
AB027469 Genomic DNA. Translation: BAA77814.1.
D86739 mRNA. Translation: BAA78032.2.
RefSeqiNP_001188751.1. NM_001201822.2.
NP_477428.1. NM_058080.4.
NP_723454.1. NM_164853.3.
NP_723455.1. NM_164854.3.
UniGeneiDm.3101.

Genome annotation databases

EnsemblMetazoaiFBtr0079800; FBpp0079400; FBgn0026439.
FBtr0079801; FBpp0079401; FBgn0026439.
FBtr0079802; FBpp0079402; FBgn0026439.
FBtr0303876; FBpp0292879; FBgn0026439.
GeneIDi34251.
KEGGidme:Dmel_CG3747.
UCSCiCG3747-RA. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF001784 mRNA. Translation: AAD09142.1.
AF165999 mRNA. Translation: AAD47829.1.
AE014134 Genomic DNA. Translation: AAF52764.1.
AE014134 Genomic DNA. Translation: AAN10691.1.
AE014134 Genomic DNA. Translation: AAN10692.1.
BT044100 mRNA. Translation: ACH92165.1.
AE014134 Genomic DNA. Translation: ADV37001.1.
AB027469 Genomic DNA. Translation: BAA77814.1.
D86739 mRNA. Translation: BAA78032.2.
RefSeqiNP_001188751.1. NM_001201822.2.
NP_477428.1. NM_058080.4.
NP_723454.1. NM_164853.3.
NP_723455.1. NM_164854.3.
UniGeneiDm.3101.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7227.FBpp0079400.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0079800; FBpp0079400; FBgn0026439.
FBtr0079801; FBpp0079401; FBgn0026439.
FBtr0079802; FBpp0079402; FBgn0026439.
FBtr0303876; FBpp0292879; FBgn0026439.
GeneIDi34251.
KEGGidme:Dmel_CG3747.
UCSCiCG3747-RA. d. melanogaster.

Organism-specific databases

CTDi34251.
FlyBaseiFBgn0026439. Eaat1.

Phylogenomic databases

eggNOGiKOG3787. Eukaryota.
COG1301. LUCA.
GeneTreeiENSGT00760000119117.
OMAiITFLRKM.
OrthoDBiEOG7RV9G2.

Enzyme and pathway databases

ReactomeiR-DME-352230. Amino acid transport across the plasma membrane.

Miscellaneous databases

GenomeRNAii34251.

Family and domain databases

Gene3Di1.10.3860.10. 1 hit.
InterProiIPR001991. Na-dicarboxylate_symporter.
IPR018107. Na-dicarboxylate_symporter_CS.
[Graphical view]
PANTHERiPTHR11958. PTHR11958. 1 hit.
PfamiPF00375. SDF. 1 hit.
[Graphical view]
PRINTSiPR00173. EDTRNSPORT.
SUPFAMiSSF118215. SSF118215. 2 hits.
PROSITEiPS00714. NA_DICARBOXYL_SYMP_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular Cloning of the cDNA for Drosophila Glutamate Transporter."
    Kawano T., Takuwa K., Nakajima T.
    Submitted (JUL-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: Canton S.Imported.
    Tissue: Whole bodyImported.
  2. "Identification and characterization of a cDNA encoding a neuronal glutamate transporter from Drosophila melanogaster."
    Seal R.P., Daniels G.M., Wolfgang W.J., Forte M.A., Amara S.G.
    Recept. Channels 6:51-64(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
  3. "Identification and structural characterization of two genes encoding glutamate transporter homologues differently expressed in the nervous system of Drosophila melanogaster."
    Besson M.T., Soustelle L., Birman S.
    FEBS Lett. 443:97-104(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
  4. Seal R.P., Daniels G.M., Amara S.G.
    Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
  5. "Genomic DNA for a Drosophila glutamate transporter."
    Kawano T.
    Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
  6. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.H., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Gabor G.L., Abril J.F., Agbayani A., An H.J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., WoodageT, Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BerkeleyImported.
  7. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BerkeleyImported.
  8. Cited for: GENOME REANNOTATION.
    Strain: BerkeleyImported.
  9. "The transposable elements of the Drosophila melanogaster euchromatin: a genomics perspective."
    Kaminker J.S., Bergman C.M., Kronmiller B., Carlson J., Svirskas R., Patel S., Frise E., Wheeler D.A., Lewis S.E., Rubin G.M., Ashburner M., Celniker S.E.
    Genome Biol. 3:RESEARCH0084.1-RESEARCH0084.20(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BerkeleyImported.
  10. "Heterochromatic sequences in a Drosophila whole-genome shotgun assembly."
    Hoskins R.A., Smith C.D., Carlson J.W., Carvalho A.B., Halpern A., Kaminker J.S., Kennedy C., Mungall C.J., Sullivan B.A., Sutton G.G., Yasuhara J.C., Wakimoto B.T., Myers E.W., Celniker S.E., Rubin G.M., Karpen G.H.
    Genome Biol. 3:RESEARCH0085-RESEARCH0085(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BerkeleyImported.
  11. "Combined evidence annotation of transposable elements in genome sequences."
    Quesneville H., Bergman C.M., Andrieu O., Autard D., Nouaud D., Ashburner M., Anxolabehere D.
    PLoS Comput. Biol. 1:166-175(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BerkeleyImported.
  12. Celniker S., Carlson J., Wan K., Frise E., Hoskins R., Park S., Svirskas R., Rubin G.
    Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
  13. "Drosophila melanogaster release 4 sequence."
    Berkeley Drosophila Genome Project
    Celniker S., Carlson J., Wan K., Pfeiffer B., Frise E., George R., Hoskins R., Stapleton M., Pacleb J., Park S., Svirskas R., Smith E., Yu C., Rubin G.
    Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
  14. "The Release 5.1 annotation of Drosophila melanogaster heterochromatin."
    Smith C.D., Shu S., Mungall C.J., Karpen G.H.
    Science 316:1586-1591(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BerkeleyImported.
  15. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BerkeleyImported.
  16. Carlson J., Booth B., Frise E., Park S., Wan K., Yu C., Celniker S.
    Submitted (SEP-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
  17. Millard Andrew
    Submitted (OCT-2015) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.

Entry informationi

Entry nameiO77062_DROME
AccessioniPrimary (citable) accession number: O77062
Entry historyi
Integrated into UniProtKB/TrEMBL: November 1, 1998
Last sequence update: November 1, 1998
Last modified: June 8, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Proteomics identificationCombined sources, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.