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Protein

Transcription factor E2F2

Gene

E2f2

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcriptional repressor that binds to E2f sites and represses E2f-regulated target genes. Binding to E2f sites requires transcription factor Dp. Acts synergistically with Rbf2 to antagonize E2f1-mediated transcriptional activation. Component of the DREAM complex, a multiprotein complex that can both act as a transcription activator or repressor depending on the context. The DREAM complex is required for recruiting E2f2 at differentiation-specific promoters and for stabilizing E2f2-Rbf complexes during S phase. During development, the complex represses transcription of developmentally controlled E2f target genes. During oogenesis, plays a role in restricting DNA synthesis to sites of chorion gene amplification in late stage ovarian follicle cells. Plays an inhibitory role in ionizing radiation (IR)-induced p53-independent apoptosis. May be involved in cell cycle exit by temporarily limiting CycE-dependent activation of E2f-regulated transcription.10 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi72 – 137Sequence analysisCuratedAdd BLAST66

GO - Molecular functioni

  • DNA binding transcription factor activity Source: InterPro
  • protein heterodimerization activity Source: UniProtKB
  • RNA polymerase II regulatory region DNA binding Source: FlyBase
  • transcription factor binding Source: FlyBase

GO - Biological processi

  • DNA endoreduplication Source: FlyBase
  • endomitotic cell cycle Source: FlyBase
  • G1/S transition of mitotic cell cycle Source: FlyBase
  • imaginal disc development Source: FlyBase
  • negative regulation of apoptotic process Source: UniProtKB
  • negative regulation of DNA replication Source: FlyBase
  • negative regulation of G1/S transition of mitotic cell cycle Source: FlyBase
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • negative regulation of transcription by RNA polymerase II Source: FlyBase
  • oogenesis Source: FlyBase
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionDNA-binding, Repressor
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-DME-1538133 G0 and Early G1
R-DME-2173796 SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription
R-DME-69231 Cyclin D associated events in G1
SignaLinkiO77051

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor E2F2
Short name:
dE2F21 Publication
Alternative name(s):
E2F transcription factor 2Imported
E2F-like transcription factor E2F2
Gene namesi
Name:E2f2
ORF Names:CG1071
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0024371 E2f2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Flies show reduced viability, with between 20 to 35% surviving until adult stages and both male and female flies showing significantly reduced fertility (PubMed:11511545). E2f1 and E2f2 double mutants die at the pupal stage during development compared with E2f1 mutants that die earlier at the larval stage, indicating that E2f1 and E2f2 functionally antagonize each other in vivo. According to PubMed:11748144, flies are viable, with males that are fully fertile and females that are partially sterile. p53 and E2f2 double mutants exhibit substantially more apoptosis at 20 and 24 hours after exposure to IR as compared to p53 single mutants, indicating that E2f2 limits IR-induced p53-independent apoptosis.4 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004204341 – 370Transcription factor E2F2Add BLAST370

Proteomic databases

PaxDbiO77051
PRIDEiO77051

Expressioni

Tissue specificityi

Ubiquitously expressed in eye disk.1 Publication

Developmental stagei

Expressed throughout embryonic development, with highest levels detected in cycling cells including in mitotically active cells during germ-band extended stages, as well as proliferating cells of the CNS and endoreduplicating cells of tissues such as the midgut at later embryonic stages. Maternal mRNA detected in syncytial stage embryos and disappears by the cellular blastoderm stage. A single transcript of 1.4 kb is detected at the highest level in 4 to 8 hour embryos, and at a relatively high level in 0 to 2 hour embryos. Lower levels of the transcript are detected in unfertilized eggs, larvae, pupae and adult.2 Publications

Gene expression databases

BgeeiFBgn0024371
ExpressionAtlasiO77051 differential
GenevisibleiO77051 DM

Interactioni

Subunit structurei

Forms a heterodimer with Dp. Interacts with Rbf/Rbf1 and Rbf2. Component of the DREAM complex, which is at least composed of Myb, Caf1, mip40, mip120, mip130, E2f2, Dp, Rbf, Rbf2, lin-52, Rpd3 and l3mbt.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
mip130Q9W5424EBI-93258,EBI-75943

GO - Molecular functioni

  • protein heterodimerization activity Source: UniProtKB
  • transcription factor binding Source: FlyBase

Protein-protein interaction databases

BioGridi61336, 77 interactors
DIPiDIP-18289N
IntActiO77051, 68 interactors
STRINGi7227.FBpp0081029

Structurei

3D structure databases

ProteinModelPortaliO77051
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni137 – 226DimerizationSequence analysisCuratedAdd BLAST90

Sequence similaritiesi

Belongs to the E2F/DP family.Sequence analysis

Phylogenomic databases

eggNOGiKOG2577 Eukaryota
ENOG410XNYI LUCA
HOGENOMiHOG000004796
InParanoidiO77051
KOiK04682
OMAiICTTQLR
OrthoDBiEOG091G09TP
PhylomeDBiO77051

Family and domain databases

Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR015633 E2F
IPR037241 E2F-DP_heterodim
IPR003316 E2F_WHTH_DNA-bd_dom
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR12081 PTHR12081, 1 hit
PfamiView protein in Pfam
PF02319 E2F_TDP, 1 hit
SMARTiView protein in SMART
SM01372 E2F_TDP, 1 hit
SUPFAMiSSF144074 SSF144074, 1 hit
SSF46785 SSF46785, 1 hit

Sequencei

Sequence statusi: Complete.

O77051-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYKRKTASIV KRDSSAAGTT SSAMMMKVDS AETSVRSQSY ESTPVSMDTS
60 70 80 90 100
PDPPTPIKSP SNSQSQSQPG QQRSVGSLVL LTQKFVDLVK ANEGSIDLKA
110 120 130 140 150
ATKILDVQKR RIYDITNVLE GIGLIDKGRH CSLVRWRGGG FNNAKDQENY
160 170 180 190 200
DLARSRTNHL KMLEDDLDRQ LEYAQRNLRY VMQDPSNRSY AYVTRDDLLD
210 220 230 240 250
IFGDDSVFTI PNYDEEVDIK RNHYELAVSL DNGSAIDIRL VTNQGKSTTN
260 270 280 290 300
PHDVDGFFDY HRLDTPSPST SSHSSEDGNA PACAGNVITD EHGYSCNPGM
310 320 330 340 350
KDEMKLLENE LTAKIIFQNY LSGHSLRRFY PDDPNLENPP LLQLNPPQED
360 370
FNFALKSDEG ICELFDVQCS
Length:370
Mass (Da):41,395
Last modified:November 1, 1998 - v1
Checksum:i20BE683AED914842
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB016824 mRNA Translation: BAA33742.1
AA202711 mRNA No translation available.
AE014134 Genomic DNA Translation: AAF53965.1
AY069118 mRNA Translation: AAL39263.1
PIRiJE0342
RefSeqiNP_001286115.1, NM_001299186.1
NP_477355.1, NM_058007.5
UniGeneiDm.2078

Genome annotation databases

EnsemblMetazoaiFBtr0081501; FBpp0081029; FBgn0024371
FBtr0346451; FBpp0312101; FBgn0024371
GeneIDi35381
KEGGidme:Dmel_CG1071
UCSCiCG1071-RA d. melanogaster

Similar proteinsi

Entry informationi

Entry nameiE2F2_DROME
AccessioniPrimary (citable) accession number: O77051
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2012
Last sequence update: November 1, 1998
Last modified: April 25, 2018
This is version 164 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health