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Protein

Endoglucanase

Gene

NtEG

Organism
Nasutitermes takasagoensis
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi225 – 2251Calcium; via carbonyl oxygenCombined sources
Metal bindingi228 – 2281CalciumCombined sources
Metal bindingi229 – 2291CalciumCombined sources
Metal bindingi269 – 2691Calcium; via carbonyl oxygenCombined sources

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

GlycosidaseUniRule annotationImported, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradationUniRule annotation, Polysaccharide degradation

Keywords - Ligandi

CalciumCombined sources, Metal-bindingCombined sources

Protein family/group databases

CAZyiGH9. Glycoside Hydrolase Family 9.

Names & Taxonomyi

Protein namesi
Recommended name:
EndoglucanaseUniRule annotation (EC:3.2.1.4UniRule annotation)
Gene namesi
Name:NtEGImported
OrganismiNasutitermes takasagoensisImported
Taxonomic identifieri62960 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraOrthopteroideaDictyopteraIsopteraTermitidaeNasutitermitinaeNasutitermes

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1616UniRule annotationAdd
BLAST
Chaini17 – 448432EndoglucanaseUniRule annotationPRO_5006776384Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi380 ↔ 387Combined sources

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1KS8X-ray1.40A17-448[»]
1KSCX-ray1.55A17-448[»]
1KSDX-ray1.60A17-448[»]
SMRiO77044. Positions 17-448.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 9 (cellulase E) family.UniRule annotationSAAS annotation

Keywords - Domaini

SignalUniRule annotation

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR001701. Glyco_hydro_9.
IPR033126. Glyco_hydro_9_Asp/Glu_AS.
[Graphical view]
PfamiPF00759. Glyco_hydro_9. 1 hit.
[Graphical view]
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiPS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O77044-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVFLCLLSA LALCQAAYDY KQVLRDSLLF YEAQRSGRLP ADQKVTWRKD
60 70 80 90 100
SALNDQGDQG QDLTGGYFDA GDFVKFGFPM AYTATVLAWG LIDFEAGYSS
110 120 130 140 150
AGALDDGRKA VKWATDYFIK AHTSQNEFYG QVGQGDADHA FWGRPEDMTM
160 170 180 190 200
ARPAYKIDTS RPGSDLAGET AAALAAASIV FRNVDGTYSN NLLTHARQLF
210 220 230 240 250
DFANNYRGKY SDSITDARNF YASADYRDEL VWAAAWLYRA TNDNTYLNTA
260 270 280 290 300
ESLYDEFGLQ NWGGGLNWDS KVSGVQVLLA KLTNKQAYKD TVQSYVNYLI
310 320 330 340 350
NNQQKTPKGL LYIDMWGTLR HAANAAFIML EAAELGLSAS SYRQFAQTQI
360 370 380 390 400
DYALGDGGRS FVCGFGSNPP TRPHHRSSSC PPAPATCDWN TFNSPDPNYH
410 420 430 440
VLSGALVGGP DQNDNYVDDR SDYVHNEVAT DYNAGFQSAL AALVALGY
Length:448
Mass (Da):49,405
Last modified:November 1, 1998 - v1
Checksum:iFF08DEBCF6806DE1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB013272 mRNA. Translation: BAA33708.1.
AB019146 Genomic DNA. Translation: BAA76619.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB013272 mRNA. Translation: BAA33708.1.
AB019146 Genomic DNA. Translation: BAA76619.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1KS8X-ray1.40A17-448[»]
1KSCX-ray1.55A17-448[»]
1KSDX-ray1.60A17-448[»]
SMRiO77044. Positions 17-448.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH9. Glycoside Hydrolase Family 9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR001701. Glyco_hydro_9.
IPR033126. Glyco_hydro_9_Asp/Glu_AS.
[Graphical view]
PfamiPF00759. Glyco_hydro_9. 1 hit.
[Graphical view]
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiPS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Metazoan cellulase genes from termites: intron/exon structures and sites of expression."
    Tokuda G., Lo N., Watanabe H., Slaytor M., Matsumoto T., Noda H.
    Biochim. Biophys. Acta 1447:146-159(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
  2. "Structure of an endoglucanase from termite, Nasutitermes takasagoensis."
    Khademi S., Guarino L.A., Watanabe H., Tokuda G., Meyer E.F.
    Acta Crystallogr. D 58:653-659(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.40 ANGSTROMS) OF 17-448 IN COMPLEX WITH CALCIUM, DISULFIDE BONDS.

Entry informationi

Entry nameiO77044_9NEOP
AccessioniPrimary (citable) accession number: O77044
Entry historyi
Integrated into UniProtKB/TrEMBL: November 1, 1998
Last sequence update: November 1, 1998
Last modified: May 11, 2016
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.