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Protein

Alpha-amylase-related protein

Gene

Amyrel

Organism
Drosophila orena (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.By similarity

Cofactori

Protein has several cofactor binding sites:
  • Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity
  • chlorideBy similarityNote: Binds 1 Cl- ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi117 – 1171CalciumBy similarity
Metal bindingi168 – 1681Calcium; via carbonyl oxygenBy similarity
Metal bindingi177 – 1771CalciumBy similarity
Binding sitei205 – 2051ChlorideBy similarity
Active sitei207 – 2071NucleophileBy similarity
Metal bindingi211 – 2111Calcium; via carbonyl oxygenBy similarity
Active sitei244 – 2441Proton donorBy similarity
Binding sitei307 – 3071ChlorideBy similarity
Sitei309 – 3091Transition state stabilizerBy similarity
Binding sitei342 – 3421ChlorideBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

Calcium, Chloride, Metal-binding

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylase-related protein (EC:3.2.1.1By similarity)
Gene namesi
Name:Amyrel
OrganismiDrosophila orena (Fruit fly)
Taxonomic identifieri7233 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora

Organism-specific databases

FlyBaseiFBgn0021266. Dore\Amyrel.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919By similarityAdd
BLAST
Chaini20 – 493474Alpha-amylase-related proteinPRO_0000001383Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei20 – 201Pyrrolidone carboxylic acidBy similarity
Disulfide bondi47 ↔ 103By similarity
Disulfide bondi156 ↔ 170By similarity
Disulfide bondi375 ↔ 381By similarity
Disulfide bondi417 ↔ 440Sequence analysis
Disulfide bondi447 ↔ 459By similarity

Keywords - PTMi

Disulfide bond, Pyrrolidone carboxylic acid

Interactioni

Subunit structurei

Monomer.By similarity

Structurei

3D structure databases

ProteinModelPortaliO77015.
SMRiO77015. Positions 20-493.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006048. A-amylase/branching_C.
IPR031319. A-amylase_C.
IPR006046. Alpha_amylase.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
[Graphical view]
PRINTSiPR00110. ALPHAAMYLASE.
SMARTiSM00642. Aamy. 1 hit.
SM00632. Aamy_C. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O77015-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFKLAFTLTL CLAGSLSLAQ HNPHWWGNRN TIVHLFEWKW LDIAQECENF
60 70 80 90 100
LGPQGFAGVQ VSPVNENIIS AGRPWWERYQ PISYKLTTRS GNEEEFGDMV
110 120 130 140 150
RRCNDVGVRI YVDVLLNHMS GDFDGVAVGT AGTEAEPRKK SFPGVPYTAQ
160 170 180 190 200
DFHPTCEITD WNDRFQVQQC ELVGLKDLNQ SSDWVRSKLI EFLDHLIELG
210 220 230 240 250
VAGFRVDAAK HMASEDLEFI YSSLSNLNIA HGFPHNSRPF IFQEVIDHGH
260 270 280 290 300
ETVSRDEYKD LGAVTEFRFS EEIGNAFRGN NALKWLQSWG TGWGFLPSGQ
310 320 330 340 350
ALTFVDNHDN QRDAGAVLSY KSPKPYKMAT AFHLAYPYGI SRVMSSFAFD
360 370 380 390 400
DHDTPPPQDA QERIISPEFD EDGACVNGWI CEHRWRQIYA MVGFKNAVRD
410 420 430 440 450
TEITGWWDNG DSQISFCRGN KGFLALNNNL YDLSQDLNTC LPAGTYCDVI
460 470 480 490
SGSLIDGSCT GKSVTVNEQG YGYIHIGSDD FDGVLALHVD AKV
Length:493
Mass (Da):55,385
Last modified:May 1, 2000 - v2
Checksum:i87EE41838B3F15B0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U96158 Genomic DNA. Translation: AAC39108.2.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U96158 Genomic DNA. Translation: AAC39108.2.

3D structure databases

ProteinModelPortaliO77015.
SMRiO77015. Positions 20-493.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

FlyBaseiFBgn0021266. Dore\Amyrel.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006048. A-amylase/branching_C.
IPR031319. A-amylase_C.
IPR006046. Alpha_amylase.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
[Graphical view]
PRINTSiPR00110. ALPHAAMYLASE.
SMARTiSM00642. Aamy. 1 hit.
SM00632. Aamy_C. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Da Lage J.-L.
    Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiAMYR_DROOR
AccessioniPrimary (citable) accession number: O77015
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: May 1, 2000
Last modified: May 11, 2016
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.