O76942 (PTP1_ENCCU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Polar tube protein 1

Alternative name(s):
Major polar tube protein
Short name=Major PTP

Gene names

Name:	PTP1
Ordered Locus Names:	ECU06_0250

Organism

Encephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite) [Reference proteome]

Taxonomic identifier

284813 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Microsporidia › Apansporoblastina › Unikaryonidae › Encephalitozoon › Encephalitozoon cuniculi

Protein attributes

Sequence length	395 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved with PTP2 and PTP3 in the formation of the polar tube through which the infectious agent is passed on to the host cell. Accounts for at least 70 percent of the mass of the polar tube.
Subunit structure	Interacts with PTP2 and PTP3. Ref.4
Subcellular location	Spore polar tube.
Developmental stage	Found in spores. Expression is high during polar tube formation.

Ontologies

Keywords
Biological process	Sporulation
Domain	Repeat Signal
PTM	Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	sporulation resulting in formation of a cellular spore Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	polar tube Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Ref.1
	Chain	23 – 395	373	Polar tube protein 1		PRO_0000022181
Regions
	Repeat	179 – 204	26	1
	Repeat	205 – 230	26	2
	Repeat	231 – 256	26	3
	Repeat	257 – 282	26	4
	Region	179 – 282	104	4 X 26 AA approximate tandem repeats
Amino acid modifications
	Glycosylation	86	1	N-linked (GlcNAc...) Potential
	Glycosylation	173	1	N-linked (GlcNAc...) Potential
	Glycosylation	311	1	N-linked (GlcNAc...) Potential
Natural variations
	Natural variant	209 – 234	26	Missing in strain: CDC:V282.
	Natural variant	210	1	I → V in strain: m104.

Sequences

	Sequence		Length	Mass (Da)
	O76942 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 8FB498209F5EA9B9	FASTA	395	39,609
	10 20 30 40 50 60 MKGISKILSA SIALMKLENV YSATALCSNA YGLTPGQQGM AQQPSYVLIP STPGTIANCA 70 80 90 100 110 120 SGSQDTYSPS PAAPTSPVTP GKTSENETSP SAPAEDVGTC KIAVLKHCDA PGTTSGTTPG 130 140 150 160 170 180 SGPCETPEQQ QPLSVISTTP AVPVTVESAQ SPSVVPVVPV VAHHQAVPGY YNNGTSGIPG 190 200 210 220 230 240 QQQILSGTLP PGATLCQGQA MPSTPGQQQI LSGTLPPGVT LCQGQATPST PGQQQVLSGT 250 260 270 280 290 300 LPPGVTLCQG QATPSTPGQQ QVLSGTLLPG ATLCQDQGMP GTSGVPGQQG QSSGQCCAPQ 310 320 330 340 350 360 IPNPVMPPSM NISGNGYPSS TAYSPNLGSL GSCVDIQKTG GTSCEQKPEK SATQYAMEAC 370 380 390 ATPTPTVIIG NSEYLVGPGM YNAINSPCNT AVQCC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"On proteins of the microsporidian invasive apparatus: complete sequence of a polar tube protein of Encephalitozoon cuniculi." Delbac F., Peyret P., Metenier G., David D., Danchin A., Vivares C.P. Mol. Microbiol. 29:825-834(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 23-42 AND 351-365, CHARACTERIZATION.
[2]	"Genome sequence and gene compaction of the eukaryote parasite Encephalitozoon cuniculi." Katinka M.D., Duprat S., Cornillot E., Metenier G., Thomarat F., Prensier G., Barbe V., Peyretaillade E., Brottier P., Wincker P., Delbac F., El Alaoui H., Peyret P., Saurin W., Gouy M., Weissenbach J., Vivares C.P. Nature 414:450-453(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GB-M1.
[3]	"Genotyping Encephalitozoon cuniculi by multilocus analyses of genes with repetitive sequences." Xiao L., Li L., Visvesvara G.S., Moura H., Didier E.S., Lal A.A. J. Clin. Microbiol. 39:2248-2253(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 12-349. Strain: CDC:V282, CDC:V385 and m104.
[4]	"The microsporidian polar tube: evidence for a third polar tube protein (PTP3) in Encephalitozoon cuniculi." Peuvel I., Peyret P., Metenier G., Vivares C.P., Delbac F. Mol. Biochem. Parasitol. 122:69-80(2002) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PTP2 AND PTP3.
[5]	"Proteomic analysis of the eukaryotic parasite Encephalitozoon cuniculi (microsporidia): a reference map for proteins expressed in late sporogonial stages." Brosson D., Kuhn L., Delbac F., Garin J., Vivares C.P., Texier C. Proteomics 6:3625-3635(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], DEVELOPMENTAL STAGE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ005666 Genomic DNA. Translation: CAA06662.1. AL590446 Genomic DNA. Translation: CAD25385.1. AF310677 Genomic DNA. Translation: AAK62814.1. AF310678 Genomic DNA. Translation: AAK62815.1. AF310679 Genomic DNA. Translation: AAK62816.1.
RefSeq	NP_585781.1. NM_001041403.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	859204.
KEGG	ecu:ECU06_0250.
Phylogenomic databases
eggNOG	NOG12793.
HOGENOM	HOG000115778.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

PTP1_ENCCU

Accession

Primary (citable) accession number: O76942
Secondary accession number(s): Q964G9, Q964H0, Q964H1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1998
Last modified:	May 1, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program