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Protein

Serine/threonine-protein phosphatase 4 catalytic subunit

Gene

Pp4-19C

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Protein phosphatase that regulates many processes such as microtubule organization at centrosomes. The probable PP4 complex Pp4-19C-PPP4R2r-flfl (PPP4C-PPP4R2-PPP4R3) is required to prevent caspase-induced cell death (in vitro).2 Publications

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi54Manganese 1By similarity1
Metal bindingi56Manganese 1By similarity1
Metal bindingi82Manganese 1By similarity1
Metal bindingi82Manganese 2By similarity1
Metal bindingi114Manganese 2By similarity1
Active sitei115Proton donorBy similarity1
Metal bindingi164Manganese 2By similarity1
Metal bindingi238Manganese 2By similarity1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • protein serine/threonine phosphatase activity Source: FlyBase

GO - Biological processi

  • microtubule-based process Source: FlyBase
  • mitotic cell cycle Source: FlyBase
  • negative regulation of smoothened signaling pathway Source: FlyBase
  • protein dephosphorylation Source: FlyBase
  • regulation of mitotic cell cycle Source: FlyBase

Keywordsi

Molecular functionHydrolase, Protein phosphatase
LigandManganese, Metal-binding

Enzyme and pathway databases

SignaLinkiO76932

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase 4 catalytic subunit (EC:3.1.3.16)
Short name:
PP4C
Gene namesi
Name:Pp4-19C
Synonyms:pp4
ORF Names:CG32505
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0023177 Pp4-19C

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003532081 – 307Serine/threonine-protein phosphatase 4 catalytic subunitAdd BLAST307

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei307Leucine methyl esterBy similarity1

Post-translational modificationi

Reversibly methyl esterified on Leu-307 by leucine carboxyl methyltransferase 1 (LCMT1) and protein phosphatase methylesterase 1 (PPME1). Carboxyl methylation influences the affinity of the catalytic subunit for the different regulatory subunits, thereby modulating the PP2A holoenzyme's substrate specificity, enzyme activity and cellular localization (By similarity).By similarity

Keywords - PTMi

Methylation

Proteomic databases

PaxDbiO76932
PRIDEiO76932

Expressioni

Gene expression databases

BgeeiFBgn0023177
ExpressionAtlasiO76932 differential
GenevisibleiO76932 DM

Interactioni

Subunit structurei

Serine/threonine-protein phosphatase 4 (PP4) occurs in different assemblies of the catalytic and one or more regulatory subunits (By similarity). Probably part of a PP4 PPP4C-PPP4R2-PPP4R3 complex containing Pp4-19C, PPP4R2r and flfl.By similarity

Protein-protein interaction databases

BioGridi69445, 17 interactors
IntActiO76932, 10 interactors
STRINGi7227.FBpp0077016

Structurei

3D structure databases

ProteinModelPortaliO76932
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0371 Eukaryota
COG0639 LUCA
InParanoidiO76932
KOiK15423
OMAiPDRMTLI
OrthoDBiEOG091G0B6S
PhylomeDBiO76932

Family and domain databases

Gene3Di3.60.21.10, 1 hit
InterProiView protein in InterPro
IPR004843 Calcineurin-like_PHP_ApaH
IPR029052 Metallo-depent_PP-like
IPR006186 Ser/Thr-sp_prot-phosphatase
PfamiView protein in Pfam
PF00149 Metallophos, 1 hit
PRINTSiPR00114 STPHPHTASE
SMARTiView protein in SMART
SM00156 PP2Ac, 1 hit
PROSITEiView protein in PROSITE
PS00125 SER_THR_PHOSPHATASE, 1 hit

Sequencei

Sequence statusi: Complete.

O76932-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDYSDLDRQ IEQLKRCEII KENEVKALCA KAREILVEEG NVQRVDSPVT
60 70 80 90 100
VCGDIHGQFY DLKELFKVGG DVPEKNYLFM GDFVDRGYYS VETFLLLLAL
110 120 130 140 150
KVRYPDRITL IRGNHESRQI TQVYGFYDEC LRKYGSTAVW RYCTEIFDYL
160 170 180 190 200
SLSAIIDGKI FCVHGGLSPS IQYLDQIRSI DRKQEVPHDG PMCDLLWSDP
210 220 230 240 250
EDQTGWGVSP RGAGYLFGSD VVSQFNRTND IDMICRAHQL VMEGFKWHFN
260 270 280 290 300
ETVLTVWSAP NYCYRCGNVA AILELNEYLH RDFVIFEAAP QESRGIPSKK

PQADYFL
Length:307
Mass (Da):35,341
Last modified:November 1, 1998 - v1
Checksum:i514FCCB93A345E2E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y14213 Genomic DNA Translation: CAA74606.1
AE014298 Genomic DNA Translation: AAF50905.1
AE014298 Genomic DNA Translation: AAN09547.1
AY113503 mRNA Translation: AAM29508.1
RefSeqiNP_001285489.1, NM_001298560.1
NP_001285490.1, NM_001298561.1
NP_001303570.1, NM_001316641.1
NP_524803.1, NM_080064.3
NP_728342.1, NM_167703.4
UniGeneiDm.1809

Genome annotation databases

EnsemblMetazoaiFBtr0077324; FBpp0077016; FBgn0023177
FBtr0077325; FBpp0077017; FBgn0023177
FBtr0345017; FBpp0311268; FBgn0023177
FBtr0346620; FBpp0312200; FBgn0023177
FBtr0445386; FBpp0401522; FBgn0023177
GeneIDi45031
KEGGidme:Dmel_CG32505

Similar proteinsi

Entry informationi

Entry nameiPP4C_DROME
AccessioniPrimary (citable) accession number: O76932
Secondary accession number(s): Q9VR98
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 4, 2008
Last sequence update: November 1, 1998
Last modified: March 28, 2018
This is version 136 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health