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O76932

- PP4C_DROME

UniProt

O76932 - PP4C_DROME

Protein

Serine/threonine-protein phosphatase 4 catalytic subunit

Gene

Pp4-19C

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 108 (01 Oct 2014)
      Sequence version 1 (01 Nov 1998)
      Previous versions | rss
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    Functioni

    Protein phosphatase that regulates many processes such as microtubule organization at centrosomes. The probable PP4 complex Pp4-19C-PPP4R2r-flfl (PPP4C-PPP4R2-PPP4R3) is required to prevent caspase-induced cell death (in vitro).2 Publications

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Cofactori

    Binds 2 manganese ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi54 – 541Manganese 1By similarity
    Metal bindingi56 – 561Manganese 1By similarity
    Metal bindingi82 – 821Manganese 1By similarity
    Metal bindingi82 – 821Manganese 2By similarity
    Metal bindingi114 – 1141Manganese 2By similarity
    Active sitei115 – 1151Proton donorBy similarity
    Metal bindingi164 – 1641Manganese 2By similarity
    Metal bindingi238 – 2381Manganese 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. protein serine/threonine phosphatase activity Source: FlyBase

    GO - Biological processi

    1. microtubule-based process Source: FlyBase
    2. mitotic cell cycle Source: FlyBase
    3. neurogenesis Source: FlyBase
    4. protein dephosphorylation Source: FlyBase
    5. regulation of mitotic cell cycle Source: FlyBase

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Ligandi

    Manganese, Metal-binding

    Enzyme and pathway databases

    SignaLinkiO76932.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein phosphatase 4 catalytic subunit (EC:3.1.3.16)
    Short name:
    PP4C
    Gene namesi
    Name:Pp4-19C
    Synonyms:pp4
    ORF Names:CG32505
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome X

    Organism-specific databases

    FlyBaseiFBgn0023177. Pp4-19C.

    Subcellular locationi

    Cytoplasm 1 Publication. Nucleus 1 Publication. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome 1 Publication

    GO - Cellular componenti

    1. centrosome Source: FlyBase
    2. cytoplasm Source: FlyBase
    3. nucleus Source: FlyBase

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 307307Serine/threonine-protein phosphatase 4 catalytic subunitPRO_0000353208Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei307 – 3071Leucine methyl esterBy similarity

    Post-translational modificationi

    Reversibly methyl esterified on Leu-307 by leucine carboxyl methyltransferase 1 (LCMT1) and protein phosphatase methylesterase 1 (PPME1). Carboxyl methylation influences the affinity of the catalytic subunit for the different regulatory subunits, thereby modulating the PP2A holoenzyme's substrate specificity, enzyme activity and cellular localization By similarity.By similarity

    Keywords - PTMi

    Methylation

    Proteomic databases

    PaxDbiO76932.
    PRIDEiO76932.

    Expressioni

    Gene expression databases

    BgeeiO76932.

    Interactioni

    Subunit structurei

    Serine/threonine-protein phosphatase 4 (PP4) occurs in different assemblies of the catalytic and one or more regulatory subunits By similarity. Probably part of a PP4 PPP4C-PPP4R2-PPP4R3 complex containing Pp4-19C, PPP4R2r and flfl.By similarity

    Protein-protein interaction databases

    BioGridi69445. 10 interactions.
    IntActiO76932. 5 interactions.
    MINTiMINT-1643247.
    STRINGi7227.FBpp0077017.

    Structurei

    3D structure databases

    ProteinModelPortaliO76932.
    SMRiO76932. Positions 6-291.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0639.
    GeneTreeiENSGT00550000074618.
    InParanoidiO76932.
    KOiK15423.
    OMAiVFNHRND.
    OrthoDBiEOG74N5H2.
    PhylomeDBiO76932.

    Family and domain databases

    Gene3Di3.60.21.10. 1 hit.
    InterProiIPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view]
    PfamiPF00149. Metallophos. 1 hit.
    [Graphical view]
    PRINTSiPR00114. STPHPHTASE.
    SMARTiSM00156. PP2Ac. 1 hit.
    [Graphical view]
    SUPFAMiSSF56300. SSF56300. 1 hit.
    PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O76932-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDYSDLDRQ IEQLKRCEII KENEVKALCA KAREILVEEG NVQRVDSPVT    50
    VCGDIHGQFY DLKELFKVGG DVPEKNYLFM GDFVDRGYYS VETFLLLLAL 100
    KVRYPDRITL IRGNHESRQI TQVYGFYDEC LRKYGSTAVW RYCTEIFDYL 150
    SLSAIIDGKI FCVHGGLSPS IQYLDQIRSI DRKQEVPHDG PMCDLLWSDP 200
    EDQTGWGVSP RGAGYLFGSD VVSQFNRTND IDMICRAHQL VMEGFKWHFN 250
    ETVLTVWSAP NYCYRCGNVA AILELNEYLH RDFVIFEAAP QESRGIPSKK 300
    PQADYFL 307
    Length:307
    Mass (Da):35,341
    Last modified:November 1, 1998 - v1
    Checksum:i514FCCB93A345E2E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y14213 Genomic DNA. Translation: CAA74606.1.
    AE014298 Genomic DNA. Translation: AAF50905.1.
    AE014298 Genomic DNA. Translation: AAN09547.1.
    AY113503 mRNA. Translation: AAM29508.1.
    RefSeqiNP_524803.1. NM_080064.2.
    NP_728342.1. NM_167703.3.
    UniGeneiDm.1809.

    Genome annotation databases

    EnsemblMetazoaiFBtr0077324; FBpp0077016; FBgn0023177.
    FBtr0077325; FBpp0077017; FBgn0023177.
    GeneIDi45031.
    KEGGidme:Dmel_CG32505.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y14213 Genomic DNA. Translation: CAA74606.1 .
    AE014298 Genomic DNA. Translation: AAF50905.1 .
    AE014298 Genomic DNA. Translation: AAN09547.1 .
    AY113503 mRNA. Translation: AAM29508.1 .
    RefSeqi NP_524803.1. NM_080064.2.
    NP_728342.1. NM_167703.3.
    UniGenei Dm.1809.

    3D structure databases

    ProteinModelPortali O76932.
    SMRi O76932. Positions 6-291.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 69445. 10 interactions.
    IntActi O76932. 5 interactions.
    MINTi MINT-1643247.
    STRINGi 7227.FBpp0077017.

    Proteomic databases

    PaxDbi O76932.
    PRIDEi O76932.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0077324 ; FBpp0077016 ; FBgn0023177 .
    FBtr0077325 ; FBpp0077017 ; FBgn0023177 .
    GeneIDi 45031.
    KEGGi dme:Dmel_CG32505.

    Organism-specific databases

    CTDi 45031.
    FlyBasei FBgn0023177. Pp4-19C.

    Phylogenomic databases

    eggNOGi COG0639.
    GeneTreei ENSGT00550000074618.
    InParanoidi O76932.
    KOi K15423.
    OMAi VFNHRND.
    OrthoDBi EOG74N5H2.
    PhylomeDBi O76932.

    Enzyme and pathway databases

    SignaLinki O76932.

    Miscellaneous databases

    GenomeRNAii 45031.
    NextBioi 837889.
    PROi O76932.

    Gene expression databases

    Bgeei O76932.

    Family and domain databases

    Gene3Di 3.60.21.10. 1 hit.
    InterProi IPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view ]
    Pfami PF00149. Metallophos. 1 hit.
    [Graphical view ]
    PRINTSi PR00114. STPHPHTASE.
    SMARTi SM00156. PP2Ac. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56300. SSF56300. 1 hit.
    PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Protein phosphatase 4 is an essential enzyme required for organisation of microtubules at centrosomes in Drosophila embryos."
      Helps N.R., Brewis N.D., Lineruth K., Davis T., Kaiser K., Cohen P.T.W.
      J. Cell Sci. 111:1331-1340(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
      Strain: Oregon-R.
    2. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley.
    3. Cited for: GENOME REANNOTATION.
      Strain: Berkeley.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Berkeley.
      Tissue: Embryo.
    5. "Depletion of protein phosphatase 4 in human cells reveals essential roles in centrosome maturation, cell migration and the regulation of Rho GTPases."
      Martin-Granados C., Philp A., Oxenham S.K., Prescott A.R., Cohen P.T.W.
      Int. J. Biochem. Cell Biol. 40:2315-2332(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROBABLE COMPONENT OF A COMPLEX WITH PPP4R2R AND FLFL, FUNCTION.

    Entry informationi

    Entry nameiPP4C_DROME
    AccessioniPrimary (citable) accession number: O76932
    Secondary accession number(s): Q9VR98
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 4, 2008
    Last sequence update: November 1, 1998
    Last modified: October 1, 2014
    This is version 108 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3