Reviewed, UniProtKB/Swiss-Prot O76924 (ARI2_DROME)

Names and origin

Protein names

Recommended name:
    Protein ariadne-2
      Short name=Ari-2

Gene names

Name:	ari-2
Synonyms:	ari2, TRIAD1
ORF Names:	CG5709

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	509 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Might act as an E3 ubiquitin-protein ligase, or as part of E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates.
Subunit structure	Interacts with UbcD10.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the RBR family. Ariadne subfamily. Contains 1 IBR-type zinc finger. Contains 2 RING-type zinc fingers.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Nucleus
Domain	Coiled coil Repeat Zinc-finger
Ligand	Metal-binding Zinc
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	modification-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	Q9VQY8	1	EBI-150281,EBI-179973
	Q9W2K0	1	EBI-150281,EBI-149290
BG4	Q9V3B4	1	EBI-150281,EBI-104309
EG:25E8.4	O46070	1	EBI-150281,EBI-122171
Ubc84D	P52487	1	EBI-150281,EBI-122135
UbcD10	O96840	1	EBI-150281,EBI-183920

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 509	509	Protein ariadne-2		PRO_0000055757
Regions
	Zinc finger	153 – 202	50	RING-type 1; atypical
	Zinc finger	222 – 284	63	IBR-type
	Zinc finger	311 – 340	30	RING-type 2
	Coiled coil	387 – 410	24	Potential
	Coiled coil	452 – 503	52	Potential
	Compositional bias	2 – 64	63	Asp/Glu-rich (acidic)
	Compositional bias	123 – 128	6	Poly-Ala

Sequences

	Sequence		Length	Mass (Da)
	O76924-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 9FB82E5ACF05E900	FASTA	509	58,627
	10 20 30 40 50 60 MDSDIEMDME SDNDGEYDDD YDYYNTGEDC DVERLDPKRA DPEYFEYECL TVEDIEKLLN 70 80 90 100 110 120 ERVEKLNTIL QITPSLAKVL LLEHQWNNVA VVEKYRQDAN ALLVTARIKP PSVAVTDTAS 130 140 150 160 170 180 TSAAAASAQL LRLGSSGYKT TASATPQYRS QMCPVCASSQ LGDKFYSLAC GHSFCKDCWT 190 200 210 220 230 240 IYFETQIFQG ISTQIGCMAQ MCNVRVPEDL VLTLVTRPVM RDKYQQFAFK DYVKSHPELR 250 260 270 280 290 300 FCPGPNCQII VQSSEISAKR AICKACHTGF CFRCGMDYHA PTDCQVIKKW LTKCADDSET 310 320 330 340 350 360 ANYISAHTKD CPKCHICIEK NGGCNHMQCF NCKHDFCWMC LGDWKTHGSE YYECSRYKDN 370 380 390 400 410 420 PNIANESVHV QAREALKKYL HYYERWENHS KSLKLEQQTI DRLRQRINSK VMNGSGTWID 430 440 450 460 470 480 WQYLFNAAAL LAKCRYTLQY TYPYAYYMEA GSRKNLFEYQ QAQLEAEIEN LSWKIERAET 490 500 TDLGDLENQM DIAEKRRTTL LKDFFPVDA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Ariadne-1: a vital Drosophila gene is required in development and defines a new conserved family of ring-finger proteins." Aguilera M., Oliveros M., Martinez-Padron M., Barbas J.A., Ferrus A. Genetics 155:1231-1244(2000) [PubMed: 10880484] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ010169 mRNA. Translation: CAA09030.1. AE013599 Genomic DNA. Translation: AAF46823.1.
RefSeq	NP_477374.1.
UniGene	Dm.4783
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:17813N.
IntAct	O76924. 31 interactions.
Proteomic databases
PRIDE	O76924.
Genome annotation databases
Ensembl	FBgn0025186. Drosophila melanogaster. [Contig view]
GeneID	37542.
KEGG	dme:Dmel_CG5709.
NMPDR	fig|7227.3.peg.6811.
Organism-specific databases
FlyBase	FBgn0025186. ari-2.
Phylogenomic databases
HOGENOM	O76924.
OMA	O76924. HEACINC.
Enzyme and pathway databases
BioCyc	DMEL-XXX-02:DMEL-XXX-02-005978-MON.
Gene expression databases
ArrayExpress	O76924.
GermOnline	CG5709. Drosophila melanogaster.
Family and domain databases
InterPro	IPR002867. Znf_C6HC. IPR001878. Znf_CCHC. IPR001841. Znf_RING. IPR017907. Znf_RING_CS. [Graphical view]
Pfam	PF01485. IBR. 1 hit. [Graphical view]
SMART	SM00647. IBR. 2 hits. SM00184. RING. 2 hits. SM00343. ZnF_C2HC. 1 hit. [Graphical view]
PROSITE	PS00518. ZF_RING_1. 1 hit. PS50089. ZF_RING_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	804182.

Entry information

Entry name

ARI2_DROME

Accession

Primary (citable) accession number: O76924

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	November 1, 1998
Last modified:	June 16, 2009
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project