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Reviewed, UniProtKB/Swiss-Prot O76856 (CATD_DICDI)

Last modified November 3, 2009. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Cathepsin D
    EC=3.4.23.5
Alternative name(s):
    Ddp44
Gene names
Name: ctsD
Synonyms: CatD
ORF Names: DDB_G0279411
OrganismDictyostelium discoideum (Slime mold) [Complete proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

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Protein attributes

Sequence length383 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Protease that may act during cell growth and/or development.

Catalytic activity

Specificity similar to, but narrower than, that of pepsin A. Does not cleave the 4-Gln-|-His-5 bond in B chain of insulin.

Subunit structure

Monomer. Ref.1

Subcellular location

Lysosome. Secreted. Ref.1

Developmental stage

Expressed in both vegetative and differentiating cells, with a higher level in vegetative cells. Ref.1

Post-translational modification

N-glycosylated on 2 out of the 3 potential sites. Glycans contain sulfated Mannose. Ref.1

Sequence similarities

Belongs to the peptidase A1 family.

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Ontologies

Keywords
   Cellular componentLysosome
Secreted
   DomainSignal
   Molecular functionAspartyl protease
Hydrolase
Protease
   PTMDisulfide bond
Glycoprotein
Zymogen
   Technical termComplete proteome
Direct protein sequencing
Gene Ontology (GO)
   Biological processproteolysis

Inferred from electronic annotation. Source: InterPro

   Cellular componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

lysosome

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionaspartic-type endopeptidase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818 Potential
Propeptide19 – 4830
PRO_0000327788
Chain49 – 383335Cathepsin D
PRO_5000147291

Sites

Active site811 By similarity
Active site2681 By similarity

Amino acid modifications

Glycosylation1181N-linked (GlcNAc...) Potential
Glycosylation2381N-linked (GlcNAc...) Potential
Glycosylation3101N-linked (GlcNAc...) Potential
Disulfide bond94 ↔ 101 By similarity
Disulfide bond259 ↔ 263 By similarity
Disulfide bond302 ↔ 339 By similarity

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Sequences

Sequence LengthMass (Da)Tools
O76856-1 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: CC8DE423AEA1A280

FASTA38341,121
        10         20         30         40         50         60 
MKLLILTLFL ATIVLAQALT VPLNFHQASR ESRRRVPQKW SNRLSALNAG TTIPISDFED 

        70         80         90        100        110        120 
AQYYGAITIG TPGQAFKVVF DTGSSNLWIP SKKCPITVVA CDLHNKYNSG ASSTYVANGT 

       130        140        150        160        170        180 
DFTIQYGSGA MSGFVSQDSV TVGSLTVKDQ LFAEATAEPG IAFDFAKFDG ILGLAFQSIS 

       190        200        210        220        230        240 
VNSIPPVFYN MLSQGLVSST LFSFWLSRTP GANGGELSFG SIDNTKYTGD ITYVPLTNET 

       250        260        270        280        290        300 
YWEFVMDDFA IDGQSAGFCG TTCHAICDSG TSLIAGPMAD ITALNEKLGA VILNGEGVFS 

       310        320        330        340        350        360 
DCSVINTLPN VTITVAGREF VLTPKEYVLE VTEFGKTECL SGFMGIELNM GNFWILGDVF 

       370        380 
ISAYYTVFDF GNKQVGFATA IQG

« Hide

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References

« Hide 'large scale' references
[1]"Characterization of Dictyostelium discoideum cathepsin D. Molecular cloning, gene disruption, endo-lysosomal localization and sugar modifications."
Journet A., Chapel A., Jehan S., Adessi C., Freeze H., Klein G., Garin J.
J. Cell Sci. 112:3833-3843(1999) [PubMed: 10523518] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], PROTEIN SEQUENCE OF 49-56; 77-92; 103-116; 269-278; 325-336 AND 370-383, SUBUNIT, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, GLYCOSYLATION.
Strain: AX2.
[2]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed: 15875012] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
+Additional computationally mapped references.

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Cross-references

Sequence databases

Y16962 mRNA. Translation: CAA76563.1.
AJ243946 Genomic DNA. Translation: CAB57223.1.
AAFI02000031 Genomic DNA. Translation: EAL67644.1.
RefSeqXP_641645.1.

3D structure databases

HSSPHSSP built from PDB template 4CMS based on UniProtKB P00794.
ModBaseSearch...

Protein-protein interaction databases

STRINGO76856.

Protein family/group databases

MEROPSA01.020.

Genome annotation databases

GeneID3392787.
GenomeReviewsGene locus ctsD in contig CM000152_GR.
KEGGddi:DDB_0215012.

Organism-specific databases

dictyBaseDDB_G0279411. ctsD.

Phylogenomic databases

OMAINEICER.

Enzyme and pathway databases

BRENDA3.4.23.5. 424.

Family and domain databases

InterProIPR001461. Peptidase_A1.
IPR001969. Peptidase_aspartic_AS.
IPR009007. Peptidase_aspartic_catalytic.
[Graphical view]
Gene3DG3DSA:2.40.70.10. Pept_Aspartc_cat. 1 hit.
PANTHERPTHR13683. Peptidase_A1. 1 hit.
PfamPF00026. Asp. 1 hit.
[Graphical view]
PRINTSPR00792. PEPSIN.
PROSITEPS00141. ASP_PROTEASE. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameCATD_DICDI
AccessionPrimary (citable) accession number: O76856
Secondary accession number(s): Q54WS2
Entry history
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: November 1, 1998
Last modified: November 3, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

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Relevant documents

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents