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Protein

ATP-dependent RNA helicase glh-4

Gene

glh-4

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable ATP-binding RNA helicase.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri570 – 58718CCHC-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri593 – 61018CCHC-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri616 – 63318CCHC-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri639 – 65618CCHC-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri665 – 68218CCHC-type 5PROSITE-ProRule annotationAdd
BLAST
Nucleotide bindingi780 – 7878ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent RNA helicase activity Source: GO_Central
  • JUN kinase binding Source: WormBase
  • RNA binding Source: UniProtKB-KW
  • RNA helicase activity Source: WormBase
  • zinc ion binding Source: InterPro

GO - Biological processi

  • gamete generation Source: WormBase
  • mRNA splicing, via spliceosome Source: GO_Central
  • regulation of cell proliferation Source: WormBase
  • regulation of gene expression Source: GO_Central
  • RNA secondary structure unwinding Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, RNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent RNA helicase glh-4 (EC:3.6.4.13)
Alternative name(s):
Germline helicase 4
Gene namesi
Name:glh-4
ORF Names:T12F5.3
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome I

Organism-specific databases

WormBaseiT12F5.3; CE29052; WBGene00001601; glh-4.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: WormBase
  • P granule Source: WormBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11561156ATP-dependent RNA helicase glh-4PRO_0000055092Add
BLAST

Proteomic databases

EPDiO76743.
PaxDbiO76743.
PRIDEiO76743.

PTM databases

iPTMnetiO76743.

Expressioni

Developmental stagei

During germline proliferation.

Gene expression databases

BgeeiWBGene00001601.

Interactioni

Subunit structurei

Interacts (via C-terminus) with kgb-1.1 Publication

GO - Molecular functioni

  • JUN kinase binding Source: WormBase

Protein-protein interaction databases

IntActiO76743. 1 interaction.
STRINGi6239.T12F5.3.2.

Structurei

3D structure databases

ProteinModelPortaliO76743.
SMRiO76743. Positions 749-1139.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini767 – 951185Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini986 – 1139154Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi736 – 76429Q motifAdd
BLAST
Motifi897 – 9004DEAD box

Sequence similaritiesi

Contains 5 CCHC-type zinc fingers.PROSITE-ProRule annotation
Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri570 – 58718CCHC-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri593 – 61018CCHC-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri616 – 63318CCHC-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri639 – 65618CCHC-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri665 – 68218CCHC-type 5PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG0335. Eukaryota.
ENOG410XNTI. LUCA.
GeneTreeiENSGT00770000120531.
InParanoidiO76743.
OrthoDBiEOG091G0LZK.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
4.10.60.10. 4 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
IPR001878. Znf_CCHC.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
PF00098. zf-CCHC. 4 hits.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00343. ZnF_C2HC. 5 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF57756. SSF57756. 2 hits.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
PS50158. ZF_CCHC. 5 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O76743-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFSDDGWGA EAEVKVAEDV PEKNVPPPVE PPRAPQSTAI KTEPERNSDE
60 70 80 90 100
PSAGFGIDPI TTSKTFGSQT TPKTEFGGAP SLSGFGGNAA AAAANKTSFD
110 120 130 140 150
QQGNGFGGAA KHGFGGVGGA PSSFGANVIP VNKPSLGHKT TGFGGEPKHV
160 170 180 190 200
SGGAFSSANN FDQQDKGFGG AASSGFGNGS MLTNQKVGLE NQTTGVGASE
210 220 230 240 250
VPTSKPSSFG QQPAVVSGFG GAAKMGFGGS SSSGFGGQKA GATESSGFPT
260 270 280 290 300
KETSTSQPGF GGDSSTGFGS GLKAGFGGHG AGAAENSGLP TETTGFGGKL
310 320 330 340 350
PSAGFGASSS NESAFGQQSK GFGGATKNGF GGDSSSSFGS GSKAGFGGTS
360 370 380 390 400
SSGIGGQKPG ATESSGFPTK ETSTSGGTFG SGFGGKPTST GFGAPSTTDS
410 420 430 440 450
SSGQQTAGFG GASKPGFGGD SSTGFGSGLK AGFGGHRAST AENSGLPTET
460 470 480 490 500
TGFGGKLPPA GFGASSSNES AFGQQSKGFG GATKNGFGGD SSNSFGKRDS
510 520 530 540 550
GFGGPQDQGF GDTDAPSKSG LGSFNTGGGA VKSAFGAAGF GSSSNFGNGN
560 570 580 590 600
TFGEPSDNQR GNWDGGERPR GCHNCGEEGH ISKECDKPKV PRFPCRNCEQ
610 620 630 640 650
LGHFASDCDQ PRVPRGPCRN CGIEGHFAVD CDQPKVPRGP CRNCGQEGHF
660 670 680 690 700
AKDCQNERVR MEPTEPCRRC AEEGHWGYEC PTRPKDLQGN FLESYDFVFT
710 720 730 740 750
PDDKMFEDAV NNDDKIDFDQ KVVASTGKVE IPDMASFDGF KILPQDLHDN
760 770 780 790 800
LKRMKMNRPT PIQRASFFPI MHGNDVVACA HTGSGKTLAF LIPFVIKLME
810 820 830 840 850
EFEKDRDVTD EKPSPRLLIV APTRELVNQT FTTARQLTYE TGLKCGLAFG
860 870 880 890 900
GYSRNANVQH LRSFSQLNIL VATMGRLQDF VNAGEVSLSK MKYIVLDEAD
910 920 930 940 950
RMVDSNDFGE EVSKIIGSPG ERTQQTVLFS ASFSEDLQSD DLPKFVKEGY
960 970 980 990 1000
TMLQVDKFGT ANEKIDQKIL PVPRTEKRDA IYKLLGIDEN TVTLLPDAPI
1010 1020 1030 1040 1050
EKQKTLIFVN SVKFCDTLAA LISSAGVSTI SMHSYQNQEQ RDRTLDDFRR
1060 1070 1080 1090 1100
GKYQCMVASN VCARGLNIAG LDHVVNYDMP DKNGFDEYVN RIGRTGRAGF
1110 1120 1130 1140 1150
TGTSTAFVDV ENDTDIIPCL VSILNEAKKE VPEWLTEGAG HQEEGGDDWN

EQEQEW
Length:1,156
Mass (Da):120,641
Last modified:August 16, 2004 - v2
Checksum:i698829D125F81064
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti136 – 1361L → P in AAC28387 (PubMed:10851135).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF079508 mRNA. Translation: AAC28387.1.
FO081191 Genomic DNA. Translation: CCD69784.1.
PIRiT32759.
T43326.
RefSeqiNP_491207.3. NM_058806.4.
UniGeneiCel.5693.

Genome annotation databases

EnsemblMetazoaiT12F5.3.1; T12F5.3.1; WBGene00001601.
T12F5.3.2; T12F5.3.2; WBGene00001601.
GeneIDi171941.
KEGGicel:CELE_T12F5.3.
UCSCiT12F5.3.1. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF079508 mRNA. Translation: AAC28387.1.
FO081191 Genomic DNA. Translation: CCD69784.1.
PIRiT32759.
T43326.
RefSeqiNP_491207.3. NM_058806.4.
UniGeneiCel.5693.

3D structure databases

ProteinModelPortaliO76743.
SMRiO76743. Positions 749-1139.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO76743. 1 interaction.
STRINGi6239.T12F5.3.2.

PTM databases

iPTMnetiO76743.

Proteomic databases

EPDiO76743.
PaxDbiO76743.
PRIDEiO76743.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiT12F5.3.1; T12F5.3.1; WBGene00001601.
T12F5.3.2; T12F5.3.2; WBGene00001601.
GeneIDi171941.
KEGGicel:CELE_T12F5.3.
UCSCiT12F5.3.1. c. elegans.

Organism-specific databases

CTDi171941.
WormBaseiT12F5.3; CE29052; WBGene00001601; glh-4.

Phylogenomic databases

eggNOGiKOG0335. Eukaryota.
ENOG410XNTI. LUCA.
GeneTreeiENSGT00770000120531.
InParanoidiO76743.
OrthoDBiEOG091G0LZK.

Miscellaneous databases

PROiO76743.

Gene expression databases

BgeeiWBGene00001601.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
4.10.60.10. 4 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
IPR001878. Znf_CCHC.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
PF00098. zf-CCHC. 4 hits.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00343. ZnF_C2HC. 5 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF57756. SSF57756. 2 hits.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
PS50158. ZF_CCHC. 5 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGLH4_CAEEL
AccessioniPrimary (citable) accession number: O76743
Secondary accession number(s): O44758
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: August 16, 2004
Last modified: September 7, 2016
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.