Reviewed, UniProtKB/Swiss-Prot O76577 (PSDE_CAEEL)

Names and origin

Protein names

Recommended name:
    26S proteasome non-ATPase regulatory subunit 14
    EC=3.1.2.15
Alternative name(s):
    26S proteasome regulatory subunit rpn11

Gene names

Name:	rpn-11
ORF Names:	K07D4.3

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	312 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Metalloprotease component of the 26S proteasome that specifically cleaves 'Lys-63'-linked polyubiquitin chains. The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. The function of the 'Lys-63'-specific deubiquitination of the proteasome is unclear By similarity.
Catalytic activity	Ubiquitin C-terminal thioester + H2O = ubiquitin + a thiol.
Subunit structure	Component of the 19S regulatory cap of the 26S proteasome By similarity.
Sequence similarities	Belongs to the peptidase M67A family. PSMD14 subfamily. Contains 1 MPN (JAB/Mov34) domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Proteasome
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	determination of adult lifespan Inferred from mutant phenotype. Source: WormBase embryonic development ending in birth or egg hatching Inferred from mutant phenotype. Source: WormBase growth Inferred from mutant phenotype. Source: WormBase locomotion Inferred from mutant phenotype. Source: WormBase modification-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW nematode larval development Inferred from mutant phenotype. Source: WormBase
Cellular component	proteasome complex Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metallopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct ubiquitin thiolesterase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
rpn-8	O61792	2	EBI-324401,EBI-324407

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 312	312	26S proteasome non-ATPase regulatory subunit 14		PRO_0000213954
Regions
	Motif	115 – 128	14	JAMM motif
Sites
	Metal binding	115	1	Zinc; catalytic By similarity
	Metal binding	117	1	Zinc; catalytic By similarity
	Metal binding	128	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	O76577-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 3EEA4CFF998D69A1	FASTA	312	34,596
	10 20 30 40 50 60 MERFLRLGGL GGNLGTFGAN PQDSNQVDTS ETVYISSLAL LKMLKHGRAG VPMEVMGLML 70 80 90 100 110 120 GEFVDDYTVN VIDVFAMPQS GTGVSVEAVD PVFQAKMLDM LKQTGRPEMV VGWYHSHPGF 130 140 150 160 170 180 GCWLSGVDIN TQQSFEALSD RAVAVVVDPI QSVKGKVVID AFRTINPQSM ALNQEPRQTT 190 200 210 220 230 240 SNLGHLQKPS IQALIHGLNR HYYSIPIAYR THDLEQKMLL NLNKLSWMDA VSVENYSKCG 250 260 270 280 290 300 EQNKEHLKAM LKLAKNYKKA LEDEKNMTDQ ELAIKNVGKM DPKRHIADEV SKMLNDNIVQ 310 SLAGMMATTS LQ

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF077534 Genomic DNA. Translation: AAC26287.1.
PIR	T33344.
RefSeq	NP_494712.1.
UniGene	Cel.4967
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	O76577. 9 interactions.
STRING	O76577.
Protein family/group databases
MEROPS	M67.001.
Genome annotation databases
Ensembl	K07D4.3.1; K07D4.3.1; K07D4.3; Caenorhabditis elegans. [Genome view] K07D4.3.2; K07D4.3.2; K07D4.3; Caenorhabditis elegans. [Genome view]
GeneID	173744.
KEGG	cel:K07D4.3.
UCSC	K07D4.3.1. c. elegans.
Organism-specific databases
CTD	173744.
WormBase	WBGene00004467. rpn-11.
WormPep	K07D4.3. CE19527. [WorfDB]
Phylogenomic databases
OMA	QDPKKHL.
Gene expression databases
ArrayExpress	O76577.
Family and domain databases
InterPro	IPR000555. Mov34_MPN_PAD1. [Graphical view]
Pfam	PF01398. Mov34. 1 hit. [Graphical view]
ProDom	PD363422. Mov34-1. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00232. JAB_MPN. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	880901.

Entry information

Entry name

PSDE_CAEEL

Accession

Primary (citable) accession number: O76577

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 31, 2004
Last sequence update:	November 1, 1998
Last modified:	November 3, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project