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Protein

Thymidylate synthase

Gene

Ts

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.

Pathwayi: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei56dUMPBy similarity1
Active sitei201NucleophileBy similarity1
Binding sitei2265,10-methylenetetrahydrofolateBy similarity1
Binding sitei234dUMPBy similarity1
Binding sitei3205,10-methylenetetrahydrofolate; via carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi181 – 182dUMP; shared with dimeric partnerBy similarity2
Nucleotide bindingi223 – 226dUMPBy similarity4
Nucleotide bindingi264 – 266dUMPBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processNucleotide biosynthesis

Enzyme and pathway databases

ReactomeiR-DME-499943 Interconversion of nucleotide di- and triphosphates
UniPathwayiUPA00575

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthase (EC:2.1.1.45)
Short name:
TS
Short name:
TSase
Gene namesi
Name:Ts
ORF Names:CG3181
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0024920 Ts

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001409051 – 321Thymidylate synthaseAdd BLAST321

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei24Phosphoserine1 Publication1
Modified residuei26Phosphoserine1 Publication1
Modified residuei39Phosphotyrosine1 Publication1
Modified residuei208Phosphotyrosine1 Publication1
Modified residuei210Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO76511
PRIDEiO76511

PTM databases

iPTMnetiO76511

Expressioni

Gene expression databases

BgeeiFBgn0024920
ExpressionAtlasiO76511 differential
GenevisibleiO76511 DM

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi59719, 1 interactor
DIPiDIP-20012N
IntActiO76511, 2 interactors
STRINGi7227.FBpp0077363

Structurei

3D structure databases

ProteinModelPortaliO76511
SMRiO76511
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidylate synthase family.Curated

Phylogenomic databases

eggNOGiKOG0673 Eukaryota
COG0207 LUCA
InParanoidiO76511
KOiK00560
OMAiKQYLDLC
OrthoDBiEOG091G0CV0
PhylomeDBiO76511

Family and domain databases

CDDicd00351 TS_Pyrimidine_HMase, 1 hit
Gene3Di3.30.572.10, 1 hit
HAMAPiMF_00008 Thymidy_synth_bact, 1 hit
InterProiView protein in InterPro
IPR023451 Thymidate_synth/dCMP_Mease
IPR036926 Thymidate_synth/dCMP_Mease_sf
IPR000398 Thymidylate_synthase
IPR020940 Thymidylate_synthase_AS
PfamiView protein in Pfam
PF00303 Thymidylat_synt, 1 hit
PRINTSiPR00108 THYMDSNTHASE
SUPFAMiSSF55831 SSF55831, 1 hit
TIGRFAMsiTIGR03284 thym_sym, 1 hit
PROSITEiView protein in PROSITE
PS00091 THYMIDYLATE_SYNTHASE, 1 hit

Sequencei

Sequence statusi: Complete.

O76511-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVLTPTKDGP DQESMPLPAD NGESPSKQQA PVNRDEMHYL DLLRHIIANG
60 70 80 90 100
EQRMDRTEVG TLSVFGSQMR FDMRNSFPLL TTKRVFFRAV AEELLWFVAG
110 120 130 140 150
KTDAKLLQAK NVHIWDGNSS REFLDKMGFT GRAVGDLGPV YGFQWRHFGA
160 170 180 190 200
QYGTCDDDYS GKGIDQLRQV IDTIRNNPSD RRIIMSAWNP LDIPKMALPP
210 220 230 240 250
CHCLAQFYVS EKRGELSCQL YQRSADMGLG VPFNIASYAL LTHMIAHVTG
260 270 280 290 300
LKPGDFVHTM GDTHVYLNHV EPLKEQLERT PRPFPKLIIK RQVQDIEDFR
310 320
FEDFQIVDYN PHPKIQMDMA V
Length:321
Mass (Da):36,657
Last modified:May 4, 2001 - v2
Checksum:iDCBC920153990CFE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti193I → M in AAC27622 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF073994 Genomic DNA Translation: AAC27622.1
AE014134 Genomic DNA Translation: AAF51176.1
RefSeqiNP_001285570.1, NM_001298641.1
NP_477367.1, NM_058019.4
UniGeneiDm.30804

Genome annotation databases

EnsemblMetazoaiFBtr0077679; FBpp0077363; FBgn0024920
FBtr0343784; FBpp0310349; FBgn0024920
GeneIDi33499
KEGGidme:Dmel_CG3181

Similar proteinsi

Entry informationi

Entry nameiTYSY_DROME
AccessioniPrimary (citable) accession number: O76511
Secondary accession number(s): Q9VQJ3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 4, 2001
Last modified: March 28, 2018
This is version 135 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health