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Protein

Thymidylate synthase

Gene

Ts

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.

Pathwayi: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei56dUMPBy similarity1
Active sitei201NucleophileBy similarity1
Binding sitei2265,10-methylenetetrahydrofolateBy similarity1
Binding sitei234dUMPBy similarity1
Binding sitei3205,10-methylenetetrahydrofolate; via carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi181 – 182dUMP; shared with dimeric partnerBy similarity2
Nucleotide bindingi223 – 226dUMPBy similarity4
Nucleotide bindingi264 – 266dUMPBy similarity3

GO - Molecular functioni

GO - Biological processi

  • dTMP biosynthetic process Source: InterPro
  • dTTP biosynthetic process Source: UniProtKB-UniPathway
  • neurogenesis Source: FlyBase
  • sensory perception of pain Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Nucleotide biosynthesis

Enzyme and pathway databases

ReactomeiR-DME-113510. E2F mediated regulation of DNA replication.
R-DME-500753. Pyrimidine biosynthesis.
R-DME-69205. G1/S-Specific Transcription.
UniPathwayiUPA00575.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthase (EC:2.1.1.45)
Short name:
TS
Short name:
TSase
Gene namesi
Name:Ts
ORF Names:CG3181
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0024920. Ts.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001409051 – 321Thymidylate synthaseAdd BLAST321

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei24Phosphoserine1 Publication1
Modified residuei26Phosphoserine1 Publication1
Modified residuei39Phosphotyrosine1 Publication1
Modified residuei208Phosphotyrosine1 Publication1
Modified residuei210Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO76511.
PRIDEiO76511.

PTM databases

iPTMnetiO76511.

Expressioni

Gene expression databases

BgeeiFBgn0024920.
ExpressionAtlasiO76511. baseline.
GenevisibleiO76511. DM.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi59719. 1 interactor.
DIPiDIP-20012N.
IntActiO76511. 1 interactor.
MINTiMINT-326941.
STRINGi7227.FBpp0077363.

Structurei

3D structure databases

ProteinModelPortaliO76511.
SMRiO76511.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidylate synthase family.Curated

Phylogenomic databases

eggNOGiKOG0673. Eukaryota.
COG0207. LUCA.
InParanoidiO76511.
KOiK00560.
OMAiIVYELLW.
OrthoDBiEOG091G0CV0.
PhylomeDBiO76511.

Family and domain databases

CDDicd00351. TS_Pyrimidine_HMase. 1 hit.
Gene3Di3.30.572.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact. 1 hit.
InterProiIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamiPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 1 hit.
PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O76511-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVLTPTKDGP DQESMPLPAD NGESPSKQQA PVNRDEMHYL DLLRHIIANG
60 70 80 90 100
EQRMDRTEVG TLSVFGSQMR FDMRNSFPLL TTKRVFFRAV AEELLWFVAG
110 120 130 140 150
KTDAKLLQAK NVHIWDGNSS REFLDKMGFT GRAVGDLGPV YGFQWRHFGA
160 170 180 190 200
QYGTCDDDYS GKGIDQLRQV IDTIRNNPSD RRIIMSAWNP LDIPKMALPP
210 220 230 240 250
CHCLAQFYVS EKRGELSCQL YQRSADMGLG VPFNIASYAL LTHMIAHVTG
260 270 280 290 300
LKPGDFVHTM GDTHVYLNHV EPLKEQLERT PRPFPKLIIK RQVQDIEDFR
310 320
FEDFQIVDYN PHPKIQMDMA V
Length:321
Mass (Da):36,657
Last modified:May 4, 2001 - v2
Checksum:iDCBC920153990CFE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti193I → M in AAC27622 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF073994 Genomic DNA. Translation: AAC27622.1.
AE014134 Genomic DNA. Translation: AAF51176.1.
RefSeqiNP_001285570.1. NM_001298641.1.
NP_477367.1. NM_058019.4.
UniGeneiDm.30804.

Genome annotation databases

EnsemblMetazoaiFBtr0077679; FBpp0077363; FBgn0024920.
FBtr0343784; FBpp0310349; FBgn0024920.
GeneIDi33499.
KEGGidme:Dmel_CG3181.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF073994 Genomic DNA. Translation: AAC27622.1.
AE014134 Genomic DNA. Translation: AAF51176.1.
RefSeqiNP_001285570.1. NM_001298641.1.
NP_477367.1. NM_058019.4.
UniGeneiDm.30804.

3D structure databases

ProteinModelPortaliO76511.
SMRiO76511.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi59719. 1 interactor.
DIPiDIP-20012N.
IntActiO76511. 1 interactor.
MINTiMINT-326941.
STRINGi7227.FBpp0077363.

PTM databases

iPTMnetiO76511.

Proteomic databases

PaxDbiO76511.
PRIDEiO76511.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0077679; FBpp0077363; FBgn0024920.
FBtr0343784; FBpp0310349; FBgn0024920.
GeneIDi33499.
KEGGidme:Dmel_CG3181.

Organism-specific databases

CTDi33499.
FlyBaseiFBgn0024920. Ts.

Phylogenomic databases

eggNOGiKOG0673. Eukaryota.
COG0207. LUCA.
InParanoidiO76511.
KOiK00560.
OMAiIVYELLW.
OrthoDBiEOG091G0CV0.
PhylomeDBiO76511.

Enzyme and pathway databases

UniPathwayiUPA00575.
ReactomeiR-DME-113510. E2F mediated regulation of DNA replication.
R-DME-500753. Pyrimidine biosynthesis.
R-DME-69205. G1/S-Specific Transcription.

Miscellaneous databases

GenomeRNAii33499.
PROiO76511.

Gene expression databases

BgeeiFBgn0024920.
ExpressionAtlasiO76511. baseline.
GenevisibleiO76511. DM.

Family and domain databases

CDDicd00351. TS_Pyrimidine_HMase. 1 hit.
Gene3Di3.30.572.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact. 1 hit.
InterProiIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamiPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 1 hit.
PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTYSY_DROME
AccessioniPrimary (citable) accession number: O76511
Secondary accession number(s): Q9VQJ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 4, 2001
Last modified: November 30, 2016
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.