Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Nitrilase and fragile histidine triad fusion protein NitFhit

Gene

nft-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cleaves A-5'-PPP-5'A to yield AMP and ADP.1 Publication

Catalytic activityi

P1-P3-bis(5'-adenosyl) triphosphate + H2O = ADP + AMP.1 Publication

Cofactori

Mn2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei541 Publication1
Active sitei1271 Publication1
Active sitei1691 Publication1
Active sitei392Tele-AMP-histidine intermediatePROSITE-ProRule annotationBy similarity1

GO - Molecular functioni

  • bis(5'-adenosyl)-triphosphatase activity Source: UniProtKB
  • hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds Source: UniProtKB
  • nucleotide binding Source: UniProtKB-KW

GO - Biological processi

  • nucleobase-containing compound metabolic process Source: UniProtKB

Keywordsi

Molecular functionHydrolase, Multifunctional enzyme
LigandNucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrilase and fragile histidine triad fusion protein NitFhit
Including the following 2 domains:
Bis(5'-adenosyl)-triphosphatase (EC:3.6.1.29)
Alternative name(s):
AP3A hydrolase
Short name:
AP3Aase
Diadenosine 5',5'''-P1,P3-triphosphate hydrolase
Dinucleosidetriphosphatase
Nitrilase homolog (EC:3.5.-.-)
Gene namesi
Name:nft-1Imported
ORF Names:Y56A3A.13
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome III

Organism-specific databases

WormBaseiY56A3A.13 ; CE22580 ; WBGene00003594 ; nft-1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001097911 – 440Nitrilase and fragile histidine triad fusion protein NitFhitAdd BLAST440

Proteomic databases

EPDiO76463
PaxDbiO76463
PeptideAtlasiO76463
PRIDEiO76463

Expressioni

Developmental stagei

Expressed in adult.1 Publication

Gene expression databases

BgeeiWBGene00003594

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

BioGridi41808, 1 interactor
DIPiDIP-26945N
IntActiO76463, 2 interactors
STRINGi6239.Y56A3A.13.2

Structurei

Secondary structure

1440
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi13 – 20Combined sources8
Helixi28 – 44Combined sources17
Beta strandi48 – 52Combined sources5
Helixi63 – 87Combined sources25
Beta strandi91 – 101Combined sources11
Beta strandi104 – 115Combined sources12
Beta strandi121 – 126Combined sources6
Beta strandi132 – 135Combined sources4
Turni136 – 138Combined sources3
Beta strandi139 – 142Combined sources4
Helixi143 – 145Combined sources3
Beta strandi157 – 159Combined sources3
Beta strandi162 – 164Combined sources3
Helixi169 – 173Combined sources5
Helixi175 – 183Combined sources9
Beta strandi187 – 190Combined sources4
Helixi197 – 215Combined sources19
Beta strandi218 – 221Combined sources4
Beta strandi223 – 229Combined sources7
Beta strandi232 – 235Combined sources4
Beta strandi239 – 241Combined sources3
Beta strandi247 – 250Combined sources4
Beta strandi253 – 255Combined sources3
Beta strandi257 – 263Combined sources7
Helixi264 – 273Combined sources10
Helixi276 – 278Combined sources3
Turni282 – 284Combined sources3
Helixi308 – 310Combined sources3
Beta strandi311 – 314Combined sources4
Beta strandi316 – 322Combined sources7
Beta strandi332 – 338Combined sources7
Helixi343 – 345Combined sources3
Helixi348 – 368Combined sources21
Beta strandi372 – 377Combined sources6
Helixi382 – 384Combined sources3
Beta strandi388 – 390Combined sources3
Beta strandi393 – 398Combined sources6
Helixi426 – 437Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1EMSX-ray2.80A/B1-440[»]
ProteinModelPortaliO76463
SMRiO76463
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO76463

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini14 – 264CN hydrolasePROSITE-ProRule annotationAdd BLAST251
Domaini297 – 405HITPROSITE-ProRule annotationAdd BLAST109

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi390 – 394Histidine triad motif5

Sequence similaritiesi

In the N-terminal section; belongs to the UPF0012 family.Sequence analysis

Phylogenomic databases

eggNOGiKOG0807 Eukaryota
KOG3379 Eukaryota
COG0388 LUCA
COG0537 LUCA
GeneTreeiENSGT00550000075099
HOGENOMiHOG000222700
InParanoidiO76463
KOiK11206
OMAiETQCFVV
OrthoDBiEOG091G0IKZ
PhylomeDBiO76463

Family and domain databases

Gene3Di3.30.428.10, 1 hit
3.60.110.10, 1 hit
InterProiView protein in InterPro
IPR003010 C-N_Hydrolase
IPR036526 C-N_Hydrolase_sf
IPR019808 Histidine_triad_CS
IPR001310 Histidine_triad_HIT
IPR011146 HIT-like
IPR036265 HIT-like_sf
IPR001110 UPF0012_CS
PfamiView protein in Pfam
PF00795 CN_hydrolase, 1 hit
PF01230 HIT, 1 hit
SUPFAMiSSF54197 SSF54197, 1 hit
SSF56317 SSF56317, 1 hit
PROSITEiView protein in PROSITE
PS50263 CN_HYDROLASE, 1 hit
PS00892 HIT_1, 1 hit
PS51084 HIT_2, 1 hit
PS01227 UPF0012, 1 hit

Sequencei

Sequence statusi: Complete.

O76463-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSTVFRRTM ATGRHFIAVC QMTSDNDLEK NFQAAKNMIE RAGEKKCEMV
60 70 80 90 100
FLPECFDFIG LNKNEQIDLA MATDCEYMEK YRELARKHNI WLSLGGLHHK
110 120 130 140 150
DPSDAAHPWN THLIIDSDGV TRAEYNKLHL FDLEIPGKVR LMESEFSKAG
160 170 180 190 200
TEMIPPVDTP IGRLGLSICY DVRFPELSLW NRKRGAQLLS FPSAFTLNTG
210 220 230 240 250
LAHWETLLRA RAIENQCYVV AAAQTGAHNP KRQSYGHSMV VDPWGAVVAQ
260 270 280 290 300
CSERVDMCFA EIDLSYVDTL REMQPVFSHR RSDLYTLHIN EKSSETGGLK
310 320 330 340 350
FARFNIPADH IFYSTPHSFV FVNLKPVTDG HVLVSPKRVV PRLTDLTDAE
360 370 380 390 400
TADLFIVAKK VQAMLEKHHN VTSTTICVQD GKDAGQTVPH VHIHILPRRA
410 420 430 440
GDFGDNEIYQ KLASHDKEPE RKPRSNEQMA EEAVVYRNLM
Length:440
Mass (Da):49,936
Last modified:November 1, 1998 - v1
Checksum:i34EED223CEF92975
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF069986 mRNA Translation: AAC39136.1
AL132860 Genomic DNA Translation: CAB60517.1
PIRiT43198
RefSeqiNP_499556.1, NM_067155.6
UniGeneiCel.19640

Genome annotation databases

EnsemblMetazoaiY56A3A.13; Y56A3A.13; WBGene00003594
GeneIDi176628
KEGGicel:CELE_Y56A3A.13
UCSCiY56A3A.13.1 c. elegans

Similar proteinsi

Entry informationi

Entry nameiNFT1_CAEEL
AccessioniPrimary (citable) accession number: O76463
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 13, 2004
Last sequence update: November 1, 1998
Last modified: March 28, 2018
This is version 112 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health