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Protein

Nitrilase and fragile histidine triad fusion protein NitFhit

Gene

nft-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Cleaves A-5'-PPP-5'A to yield AMP and ADP.1 Publication

Catalytic activityi

P(1)-P(3)-bis(5'-adenosyl) triphosphate + H2O = ADP + AMP.1 Publication

Cofactori

Mn2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei541 Publication1
Active sitei1271 Publication1
Active sitei1691 Publication1
Active sitei392Tele-AMP-histidine intermediatePROSITE-ProRule annotationBy similarity1

GO - Molecular functioni

  • bis(5'-adenosyl)-triphosphatase activity Source: UniProtKB
  • hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds Source: UniProtKB
  • nucleotide binding Source: UniProtKB-KW

GO - Biological processi

  • nucleobase-containing compound metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrilase and fragile histidine triad fusion protein NitFhit
Including the following 2 domains:
Bis(5'-adenosyl)-triphosphatase (EC:3.6.1.29)
Alternative name(s):
AP3A hydrolase
Short name:
AP3Aase
Diadenosine 5',5'''-P1,P3-triphosphate hydrolase
Dinucleosidetriphosphatase
Nitrilase homolog (EC:3.5.-.-)
Gene namesi
Name:nft-1Imported
ORF Names:Y56A3A.13
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome III

Organism-specific databases

WormBaseiY56A3A.13; CE22580; WBGene00003594; nft-1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001097911 – 440Nitrilase and fragile histidine triad fusion protein NitFhitAdd BLAST440

Proteomic databases

EPDiO76463.
PaxDbiO76463.
PeptideAtlasiO76463.
PRIDEiO76463.

Expressioni

Developmental stagei

Expressed in adult.1 Publication

Gene expression databases

BgeeiWBGene00003594.

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

BioGridi41808. 1 interactor.
DIPiDIP-26945N.
IntActiO76463. 2 interactors.
MINTiMINT-1054039.
STRINGi6239.Y56A3A.13.2.

Structurei

Secondary structure

1440
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi13 – 20Combined sources8
Helixi28 – 44Combined sources17
Beta strandi48 – 52Combined sources5
Helixi63 – 87Combined sources25
Beta strandi91 – 101Combined sources11
Beta strandi104 – 115Combined sources12
Beta strandi121 – 126Combined sources6
Beta strandi132 – 135Combined sources4
Turni136 – 138Combined sources3
Beta strandi139 – 142Combined sources4
Helixi143 – 145Combined sources3
Beta strandi157 – 159Combined sources3
Beta strandi162 – 164Combined sources3
Helixi169 – 173Combined sources5
Helixi175 – 183Combined sources9
Beta strandi187 – 190Combined sources4
Helixi197 – 215Combined sources19
Beta strandi218 – 221Combined sources4
Beta strandi223 – 229Combined sources7
Beta strandi232 – 235Combined sources4
Beta strandi239 – 241Combined sources3
Beta strandi247 – 250Combined sources4
Beta strandi253 – 255Combined sources3
Beta strandi257 – 263Combined sources7
Helixi264 – 273Combined sources10
Helixi276 – 278Combined sources3
Turni282 – 284Combined sources3
Helixi308 – 310Combined sources3
Beta strandi311 – 314Combined sources4
Beta strandi316 – 322Combined sources7
Beta strandi332 – 338Combined sources7
Helixi343 – 345Combined sources3
Helixi348 – 368Combined sources21
Beta strandi372 – 377Combined sources6
Helixi382 – 384Combined sources3
Beta strandi388 – 390Combined sources3
Beta strandi393 – 398Combined sources6
Helixi426 – 437Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1EMSX-ray2.80A/B1-440[»]
ProteinModelPortaliO76463.
SMRiO76463.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO76463.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini15 – 286CN hydrolasePROSITE-ProRule annotation1 PublicationAdd BLAST272
Domaini297 – 405HITPROSITE-ProRule annotationAdd BLAST109

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi390 – 394Histidine triad motif5

Sequence similaritiesi

In the N-terminal section; belongs to the UPF0012 family.Sequence analysis
Contains 1 CN hydrolase domain.PROSITE-ProRule annotation
Contains 1 HIT domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0807. Eukaryota.
KOG3379. Eukaryota.
COG0388. LUCA.
COG0537. LUCA.
GeneTreeiENSGT00550000075099.
HOGENOMiHOG000222700.
InParanoidiO76463.
OMAiETQCFVV.
OrthoDBiEOG091G0IKZ.
PhylomeDBiO76463.

Family and domain databases

Gene3Di3.30.428.10. 1 hit.
3.60.110.10. 1 hit.
InterProiIPR003010. C-N_Hydrolase.
IPR019808. Histidine_triad_CS.
IPR001310. Histidine_triad_HIT.
IPR011146. HIT-like.
IPR001110. UPF0012_CS.
[Graphical view]
PfamiPF00795. CN_hydrolase. 1 hit.
PF01230. HIT. 1 hit.
[Graphical view]
SUPFAMiSSF54197. SSF54197. 1 hit.
SSF56317. SSF56317. 1 hit.
PROSITEiPS50263. CN_HYDROLASE. 1 hit.
PS00892. HIT_1. 1 hit.
PS51084. HIT_2. 1 hit.
PS01227. UPF0012. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O76463-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSTVFRRTM ATGRHFIAVC QMTSDNDLEK NFQAAKNMIE RAGEKKCEMV
60 70 80 90 100
FLPECFDFIG LNKNEQIDLA MATDCEYMEK YRELARKHNI WLSLGGLHHK
110 120 130 140 150
DPSDAAHPWN THLIIDSDGV TRAEYNKLHL FDLEIPGKVR LMESEFSKAG
160 170 180 190 200
TEMIPPVDTP IGRLGLSICY DVRFPELSLW NRKRGAQLLS FPSAFTLNTG
210 220 230 240 250
LAHWETLLRA RAIENQCYVV AAAQTGAHNP KRQSYGHSMV VDPWGAVVAQ
260 270 280 290 300
CSERVDMCFA EIDLSYVDTL REMQPVFSHR RSDLYTLHIN EKSSETGGLK
310 320 330 340 350
FARFNIPADH IFYSTPHSFV FVNLKPVTDG HVLVSPKRVV PRLTDLTDAE
360 370 380 390 400
TADLFIVAKK VQAMLEKHHN VTSTTICVQD GKDAGQTVPH VHIHILPRRA
410 420 430 440
GDFGDNEIYQ KLASHDKEPE RKPRSNEQMA EEAVVYRNLM
Length:440
Mass (Da):49,936
Last modified:November 1, 1998 - v1
Checksum:i34EED223CEF92975
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF069986 mRNA. Translation: AAC39136.1.
AL132860 Genomic DNA. Translation: CAB60517.1.
PIRiT43198.
RefSeqiNP_499556.1. NM_067155.6.
UniGeneiCel.19640.

Genome annotation databases

EnsemblMetazoaiY56A3A.13; Y56A3A.13; WBGene00003594.
GeneIDi176628.
KEGGicel:CELE_Y56A3A.13.
UCSCiY56A3A.13.1. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF069986 mRNA. Translation: AAC39136.1.
AL132860 Genomic DNA. Translation: CAB60517.1.
PIRiT43198.
RefSeqiNP_499556.1. NM_067155.6.
UniGeneiCel.19640.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1EMSX-ray2.80A/B1-440[»]
ProteinModelPortaliO76463.
SMRiO76463.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi41808. 1 interactor.
DIPiDIP-26945N.
IntActiO76463. 2 interactors.
MINTiMINT-1054039.
STRINGi6239.Y56A3A.13.2.

Proteomic databases

EPDiO76463.
PaxDbiO76463.
PeptideAtlasiO76463.
PRIDEiO76463.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiY56A3A.13; Y56A3A.13; WBGene00003594.
GeneIDi176628.
KEGGicel:CELE_Y56A3A.13.
UCSCiY56A3A.13.1. c. elegans.

Organism-specific databases

CTDi176628.
WormBaseiY56A3A.13; CE22580; WBGene00003594; nft-1.

Phylogenomic databases

eggNOGiKOG0807. Eukaryota.
KOG3379. Eukaryota.
COG0388. LUCA.
COG0537. LUCA.
GeneTreeiENSGT00550000075099.
HOGENOMiHOG000222700.
InParanoidiO76463.
OMAiETQCFVV.
OrthoDBiEOG091G0IKZ.
PhylomeDBiO76463.

Miscellaneous databases

EvolutionaryTraceiO76463.
PROiO76463.

Gene expression databases

BgeeiWBGene00003594.

Family and domain databases

Gene3Di3.30.428.10. 1 hit.
3.60.110.10. 1 hit.
InterProiIPR003010. C-N_Hydrolase.
IPR019808. Histidine_triad_CS.
IPR001310. Histidine_triad_HIT.
IPR011146. HIT-like.
IPR001110. UPF0012_CS.
[Graphical view]
PfamiPF00795. CN_hydrolase. 1 hit.
PF01230. HIT. 1 hit.
[Graphical view]
SUPFAMiSSF54197. SSF54197. 1 hit.
SSF56317. SSF56317. 1 hit.
PROSITEiPS50263. CN_HYDROLASE. 1 hit.
PS00892. HIT_1. 1 hit.
PS51084. HIT_2. 1 hit.
PS01227. UPF0012. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNFT1_CAEEL
AccessioniPrimary (citable) accession number: O76463
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2004
Last sequence update: November 1, 1998
Last modified: November 2, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Multifunctional enzyme, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.