Skip Header

 
Contribute Send feedback
Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot O76463 (NFT1_CAEEL)

Last modified June 16, 2009. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein namesRecommended name:
    Nitrilase and fragile histidine triad fusion protein NitFhit
Including the following 2 domains:
    1- Recommended name:
            Bis(5'-adenosyl)-triphosphatase
              EC=3.6.1.29
        Alternative name(s):
            Diadenosine 5',5'''-P1,P3-triphosphate hydrolase
            Dinucleosidetriphosphatase
            AP3A hydrolase
              Short name=AP3Aase
    2- Recommended name:
            Nitrilase homolog
              EC=3.5.-.-
Gene names
Name: nft-1
ORF Names: Y56A3A.13
OrganismCaenorhabditis elegans [Complete proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Hide | Top

Protein attributes

Sequence length440 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

Hide | Top

General annotation (Comments)

Function

Cleaves A-5'-PPP-5'A to yield AMP and ADP. Ref.3

Catalytic activity

P(1)-P(3)-bis(5'-adenosyl) triphosphate + H2O = ADP + AMP. Ref.3

Cofactor

Manganese By similarity. UniProtKB O76464

Subunit structure

Homotetramer. Ref.3

Developmental stage

Expressed in adult. Ref.1

Sequence similarities

In the N-terminal section; belongs to the UPF0012 family.

Contains 1 CN hydrolase domain.

Contains 1 HIT domain.

Hide | Top

Binary interactions

With

Entry

#Exp.

IntAct

Notes

dyb-1Q9Y0481EBI-322796,EBI-322698

Hide | Top

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 440440Nitrilase and fragile histidine triad fusion protein NitFhit
PRO_0000109791

Regions

Domain15 – 286272CN hydrolase Ref.3
Domain297 – 405109HIT
Region378 – 39518Binding to substrate; phosphate linker By similarity
Motif390 – 3945Histidine triad motif By similarity UniProtKB P49789

Sites

Active site541 Probable Ref.1
Active site1271 Probable Ref.1
Active site1691 Probable Ref.1
Active site3921Tele-AMP-histidine intermediate By similarity UniProtKB P49789

Secondary structure

1 ........................................................................ 440
Helix Strand Turn

Details...

Hide | Top

Sequences

Sequence LengthMass (Da)Tools
O76463-1 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: 34EED223CEF92975

FASTA44049,936
        10         20         30         40         50         60 
MLSTVFRRTM ATGRHFIAVC QMTSDNDLEK NFQAAKNMIE RAGEKKCEMV FLPECFDFIG 

        70         80         90        100        110        120 
LNKNEQIDLA MATDCEYMEK YRELARKHNI WLSLGGLHHK DPSDAAHPWN THLIIDSDGV 

       130        140        150        160        170        180 
TRAEYNKLHL FDLEIPGKVR LMESEFSKAG TEMIPPVDTP IGRLGLSICY DVRFPELSLW 

       190        200        210        220        230        240 
NRKRGAQLLS FPSAFTLNTG LAHWETLLRA RAIENQCYVV AAAQTGAHNP KRQSYGHSMV 

       250        260        270        280        290        300 
VDPWGAVVAQ CSERVDMCFA EIDLSYVDTL REMQPVFSHR RSDLYTLHIN EKSSETGGLK 

       310        320        330        340        350        360 
FARFNIPADH IFYSTPHSFV FVNLKPVTDG HVLVSPKRVV PRLTDLTDAE TADLFIVAKK 

       370        380        390        400        410        420 
VQAMLEKHHN VTSTTICVQD GKDAGQTVPH VHIHILPRRA GDFGDNEIYQ KLASHDKEPE 

       430        440 
RKPRSNEQMA EEAVVYRNLM

« Hide

Hide | Top

References

« Hide 'large scale' references
[1]"Nitrilase and Fhit homologs are encoded as fusion proteins in Drosophila melanogaster and Caenorhabditis elegans."
Pekarsky Y., Campiglio M., Siprashvili Z., Druck T., Sedkov Y., Tillib S., Draganescu A., Wermuth P., Rothman J.H., Huebner K., Buchberg A.M., Mazo A., Brenner C., Croce C.M.
Proc. Natl. Acad. Sci. U.S.A. 95:8744-8749(1998) [PubMed: 9671749] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
[2]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Bristol N2.
[3]"Crystal structure of the worm NitFhit Rosetta stone protein reveals a Nit tetramer binding two Fhit dimers."
Pace H.C., Hodawadekar S.C., Draganescu A., Huang J., Bieganowski P., Pekarsky Y., Croce C.M., Brenner C.
Curr. Biol. 10:907-917(2000) [PubMed: 10959838] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS), PROTEIN SEQUENCE OF N-TERMINUS, ACTIVE SITES, FUNCTION, CATALYTIC ACTIVITY, SUBUNIT.

Hide | Top

Cross-references

Sequence databases

AF069986 mRNA. Translation: AAC39136.1.
AL132860 Genomic DNA. Translation: CAB60517.1.
PIRT43198.
RefSeqNP_499556.1.
UniGeneCel.19640

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1EMSX-ray2.80A/B1-440[»]
ModBaseSearch...

Protein-protein interaction databases

DIPDIP:26945N.
IntActO76463. 1 interaction.

Genome annotation databases

EnsemblY56A3A.13. Caenorhabditis elegans. [Contig view]
GeneID176628.
KEGGcel:Y56A3A.13.
NMPDRfig|6239.3.peg.11788.

Organism-specific databases

WormBaseWBGene00003594. nft-1.
WormPepY56A3A.13. CE22580. [WorfDB]

Phylogenomic databases

OMAO76463. ETQCFVV.

Enzyme and pathway databases

BRENDA3.6.1.29. 672.

Gene expression databases

ArrayExpressO76463.

Family and domain databases

InterProIPR011151. His_triad_motif.
IPR019808. Histidine_triad_CS.
IPR001310. Histidine_triad_HIT.
IPR003010. Ntlse/CNhydtse.
IPR001110. UPF0012_CS.
[Graphical view]
Gene3DG3DSA:3.30.428.10. His_triad_motif. 1 hit.
G3DSA:3.60.110.10. Ntlse/CNhydtse. 1 hit.
PfamPF00795. CN_hydrolase. 1 hit.
PF01230. HIT. 1 hit.
[Graphical view]
PROSITEPS50263. CN_HYDROLASE. 1 hit.
PS00892. HIT_1. 1 hit.
PS51084. HIT_2. 1 hit.
PS01227. UPF0012. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio893366.

Hide | Top

Entry information

Entry nameNFT1_CAEEL
AccessionPrimary (citable) accession number: O76463
Entry history
Integrated into UniProtKB/Swiss-Prot: September 13, 2004
Last sequence update: November 1, 1998
Last modified: June 16, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectCaenorhabditis annotation project

Hide | Top

Relevant documents

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormPep

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Uncharacterized protein families (UPF)

List of uncharacterized protein family (UPF) entries

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents