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Protein

Nitrilase and fragile histidine triad fusion protein NitFhit

Gene

nft-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Cleaves A-5'-PPP-5'A to yield AMP and ADP.1 Publication

Catalytic activityi

P(1)-P(3)-bis(5'-adenosyl) triphosphate + H2O = ADP + AMP.1 Publication

Cofactori

Mn2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei54 – 5411 Publication
Active sitei127 – 12711 Publication
Active sitei169 – 16911 Publication
Active sitei392 – 3921Tele-AMP-histidine intermediatePROSITE-ProRule annotationBy similarity

GO - Molecular functioni

  • bis(5'-adenosyl)-triphosphatase activity Source: UniProtKB
  • hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds Source: UniProtKB
  • nucleotide binding Source: UniProtKB-KW

GO - Biological processi

  • nucleobase-containing compound metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrilase and fragile histidine triad fusion protein NitFhit
Including the following 2 domains:
Bis(5'-adenosyl)-triphosphatase (EC:3.6.1.29)
Alternative name(s):
AP3A hydrolase
Short name:
AP3Aase
Diadenosine 5',5'''-P1,P3-triphosphate hydrolase
Dinucleosidetriphosphatase
Nitrilase homolog (EC:3.5.-.-)
Gene namesi
Name:nft-1Imported
ORF Names:Y56A3A.13
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome III

Organism-specific databases

WormBaseiY56A3A.13; CE22580; WBGene00003594; nft-1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 440440Nitrilase and fragile histidine triad fusion protein NitFhitPRO_0000109791Add
BLAST

Proteomic databases

EPDiO76463.
PaxDbiO76463.

Expressioni

Developmental stagei

Expressed in adult.1 Publication

Gene expression databases

BgeeiWBGene00003594.

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

BioGridi41808. 1 interaction.
DIPiDIP-26945N.
IntActiO76463. 2 interactions.
MINTiMINT-1054039.
STRINGi6239.Y56A3A.13.2.

Structurei

Secondary structure

1
440
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi13 – 208Combined sources
Helixi28 – 4417Combined sources
Beta strandi48 – 525Combined sources
Helixi63 – 8725Combined sources
Beta strandi91 – 10111Combined sources
Beta strandi104 – 11512Combined sources
Beta strandi121 – 1266Combined sources
Beta strandi132 – 1354Combined sources
Turni136 – 1383Combined sources
Beta strandi139 – 1424Combined sources
Helixi143 – 1453Combined sources
Beta strandi157 – 1593Combined sources
Beta strandi162 – 1643Combined sources
Helixi169 – 1735Combined sources
Helixi175 – 1839Combined sources
Beta strandi187 – 1904Combined sources
Helixi197 – 21519Combined sources
Beta strandi218 – 2214Combined sources
Beta strandi223 – 2297Combined sources
Beta strandi232 – 2354Combined sources
Beta strandi239 – 2413Combined sources
Beta strandi247 – 2504Combined sources
Beta strandi253 – 2553Combined sources
Beta strandi257 – 2637Combined sources
Helixi264 – 27310Combined sources
Helixi276 – 2783Combined sources
Turni282 – 2843Combined sources
Helixi308 – 3103Combined sources
Beta strandi311 – 3144Combined sources
Beta strandi316 – 3227Combined sources
Beta strandi332 – 3387Combined sources
Helixi343 – 3453Combined sources
Helixi348 – 36821Combined sources
Beta strandi372 – 3776Combined sources
Helixi382 – 3843Combined sources
Beta strandi388 – 3903Combined sources
Beta strandi393 – 3986Combined sources
Helixi426 – 43712Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1EMSX-ray2.80A/B1-440[»]
ProteinModelPortaliO76463.
SMRiO76463. Positions 10-440.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO76463.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini15 – 286272CN hydrolasePROSITE-ProRule annotation1 PublicationAdd
BLAST
Domaini297 – 405109HITPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi390 – 3945Histidine triad motif

Sequence similaritiesi

In the N-terminal section; belongs to the UPF0012 family.Sequence analysis
Contains 1 CN hydrolase domain.PROSITE-ProRule annotation
Contains 1 HIT domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0807. Eukaryota.
KOG3379. Eukaryota.
COG0388. LUCA.
COG0537. LUCA.
GeneTreeiENSGT00550000075099.
HOGENOMiHOG000222700.
InParanoidiO76463.
KOiK01522.
OMAiETQCFVV.
OrthoDBiEOG091G0IKZ.
PhylomeDBiO76463.

Family and domain databases

Gene3Di3.30.428.10. 1 hit.
3.60.110.10. 1 hit.
InterProiIPR003010. C-N_Hydrolase.
IPR019808. Histidine_triad_CS.
IPR001310. Histidine_triad_HIT.
IPR011146. HIT-like.
IPR001110. UPF0012_CS.
[Graphical view]
PfamiPF00795. CN_hydrolase. 1 hit.
PF01230. HIT. 1 hit.
[Graphical view]
SUPFAMiSSF54197. SSF54197. 1 hit.
SSF56317. SSF56317. 1 hit.
PROSITEiPS50263. CN_HYDROLASE. 1 hit.
PS00892. HIT_1. 1 hit.
PS51084. HIT_2. 1 hit.
PS01227. UPF0012. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O76463-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSTVFRRTM ATGRHFIAVC QMTSDNDLEK NFQAAKNMIE RAGEKKCEMV
60 70 80 90 100
FLPECFDFIG LNKNEQIDLA MATDCEYMEK YRELARKHNI WLSLGGLHHK
110 120 130 140 150
DPSDAAHPWN THLIIDSDGV TRAEYNKLHL FDLEIPGKVR LMESEFSKAG
160 170 180 190 200
TEMIPPVDTP IGRLGLSICY DVRFPELSLW NRKRGAQLLS FPSAFTLNTG
210 220 230 240 250
LAHWETLLRA RAIENQCYVV AAAQTGAHNP KRQSYGHSMV VDPWGAVVAQ
260 270 280 290 300
CSERVDMCFA EIDLSYVDTL REMQPVFSHR RSDLYTLHIN EKSSETGGLK
310 320 330 340 350
FARFNIPADH IFYSTPHSFV FVNLKPVTDG HVLVSPKRVV PRLTDLTDAE
360 370 380 390 400
TADLFIVAKK VQAMLEKHHN VTSTTICVQD GKDAGQTVPH VHIHILPRRA
410 420 430 440
GDFGDNEIYQ KLASHDKEPE RKPRSNEQMA EEAVVYRNLM
Length:440
Mass (Da):49,936
Last modified:November 1, 1998 - v1
Checksum:i34EED223CEF92975
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF069986 mRNA. Translation: AAC39136.1.
AL132860 Genomic DNA. Translation: CAB60517.1.
PIRiT43198.
RefSeqiNP_499556.1. NM_067155.6.
UniGeneiCel.19640.

Genome annotation databases

EnsemblMetazoaiY56A3A.13; Y56A3A.13; WBGene00003594.
GeneIDi176628.
KEGGicel:CELE_Y56A3A.13.
UCSCiY56A3A.13.1. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF069986 mRNA. Translation: AAC39136.1.
AL132860 Genomic DNA. Translation: CAB60517.1.
PIRiT43198.
RefSeqiNP_499556.1. NM_067155.6.
UniGeneiCel.19640.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1EMSX-ray2.80A/B1-440[»]
ProteinModelPortaliO76463.
SMRiO76463. Positions 10-440.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi41808. 1 interaction.
DIPiDIP-26945N.
IntActiO76463. 2 interactions.
MINTiMINT-1054039.
STRINGi6239.Y56A3A.13.2.

Proteomic databases

EPDiO76463.
PaxDbiO76463.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiY56A3A.13; Y56A3A.13; WBGene00003594.
GeneIDi176628.
KEGGicel:CELE_Y56A3A.13.
UCSCiY56A3A.13.1. c. elegans.

Organism-specific databases

CTDi176628.
WormBaseiY56A3A.13; CE22580; WBGene00003594; nft-1.

Phylogenomic databases

eggNOGiKOG0807. Eukaryota.
KOG3379. Eukaryota.
COG0388. LUCA.
COG0537. LUCA.
GeneTreeiENSGT00550000075099.
HOGENOMiHOG000222700.
InParanoidiO76463.
KOiK01522.
OMAiETQCFVV.
OrthoDBiEOG091G0IKZ.
PhylomeDBiO76463.

Miscellaneous databases

EvolutionaryTraceiO76463.
PROiO76463.

Gene expression databases

BgeeiWBGene00003594.

Family and domain databases

Gene3Di3.30.428.10. 1 hit.
3.60.110.10. 1 hit.
InterProiIPR003010. C-N_Hydrolase.
IPR019808. Histidine_triad_CS.
IPR001310. Histidine_triad_HIT.
IPR011146. HIT-like.
IPR001110. UPF0012_CS.
[Graphical view]
PfamiPF00795. CN_hydrolase. 1 hit.
PF01230. HIT. 1 hit.
[Graphical view]
SUPFAMiSSF54197. SSF54197. 1 hit.
SSF56317. SSF56317. 1 hit.
PROSITEiPS50263. CN_HYDROLASE. 1 hit.
PS00892. HIT_1. 1 hit.
PS51084. HIT_2. 1 hit.
PS01227. UPF0012. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNFT1_CAEEL
AccessioniPrimary (citable) accession number: O76463
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2004
Last sequence update: November 1, 1998
Last modified: September 7, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Multifunctional enzyme, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.