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Protein

26S protease regulatory subunit 6A

Gene

rpt-5

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins (PubMed:27528192). This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required (By similarity). Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair (By similarity). Belongs to the heterohexameric ring of AAA (ATPases associated with diverse cellular activities) proteins that unfolds ubiquitinated target proteins that are concurrently translocated into a proteolytic chamber and degraded into peptides (By similarity).By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi218 – 225ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-CEL-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-CEL-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-CEL-382556. ABC-family proteins mediated transport.
R-CEL-4641258. Degradation of DVL.
R-CEL-5689603. UCH proteinases.
R-CEL-5689880. Ub-specific processing proteases.
R-CEL-6798695. Neutrophil degranulation.
R-CEL-68949. Orc1 removal from chromatin.
R-CEL-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-CEL-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-CEL-8854050. FBXL7 down-regulates AURKA during mitotic entry and in early mitosis.
R-CEL-8941858. Regulation of RUNX3 expression and activity.
R-CEL-8948751. Regulation of PTEN stability and activity.
R-CEL-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
26S protease regulatory subunit 6ABy similarity
Alternative name(s):
Proteasome regulatory particle ATPase-like protein 5Imported
Gene namesi
Name:rpt-5Imported
ORF Names:F56H1.4Imported
OrganismiCaenorhabditis elegansImported
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome I

Organism-specific databases

WormBaseiF56H1.4; CE17915; WBGene00004505; rpt-5.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

Pathology & Biotechi

Disruption phenotypei

RNAi-mediated knockdown results in larval arrest and up-regulation of the aspartic protease ddi-1, indicative of proteasomal dysfunction. RNAi-mediated knockdown in a ddi-1, png-1, sel-11 or sel-1 mutant background results in reduced or failed expression of the proteasomal subunit rpt-3.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004423351 – 43026S protease regulatory subunit 6ACuratedAdd BLAST430

Proteomic databases

EPDiO76371.
PaxDbiO76371.
PeptideAtlasiO76371.

Expressioni

Gene expression databases

BgeeiWBGene00004505.

Interactioni

Subunit structurei

Component of the 19S proteasome regulatory particle complex. The 26S proteasome consists of a 20S core particle (CP) and two 19S regulatory subunits (RP). The regulatory particle is made of a lid composed of 9 subunits, a base containing 6 ATPases including the PSMC3 homolog rpt-5 and few additional components.By similarity

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

IntActiO76371. 4 interactors.
MINTiMINT-211164.
STRINGi6239.F56H1.4.2.

Structurei

3D structure databases

ProteinModelPortaliO76371.
SMRiO76371.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AAA ATPase family.Curated

Phylogenomic databases

eggNOGiKOG0652. Eukaryota.
COG1222. LUCA.
GeneTreeiENSGT00730000111070.
HOGENOMiHOG000225143.
InParanoidiO76371.
KOiK03065.
OMAiRACAAQS.
OrthoDBiEOG091G07E0.
PhylomeDBiO76371.

Family and domain databases

InterProiView protein in InterPro
IPR005937. 26S_Psome_P45-like.
IPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR027417. P-loop_NTPase.
IPR035254. PSMC3.
PANTHERiPTHR23073:SF7. PTHR23073:SF7. 1 hit.
PfamiView protein in Pfam
PF00004. AAA. 1 hit.
SMARTiView protein in SMART
SM00382. AAA. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01242. 26Sp45. 1 hit.
PROSITEiView protein in PROSITE
PS00674. AAA. 1 hit.

Sequencei

Sequence statusi: Complete.

O76371-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQTPPPKDG KNPEAMEVED AIDEEILKMS TEDLKSRTHL LDNEIRIMRS
60 70 80 90 100
EVQRINHSAT TLKERIKENT ERIKVNKTLP YLVSNVVELL DLEDNTEEEG
110 120 130 140 150
ANVDLDAQKT KCAVIKTSTR ATYFLPVVGL VDPDELKPGD LVGVNKDSYL
160 170 180 190 200
ILEKLPAEYD SRVKAMEVDE RPTEQYSDIG GCDKQIQELI EAVVLPMTHK
210 220 230 240 250
DRFVNLGIHP PKGVLMYGPP GTGKTMMARA VAAQTKSTFL KLAGPQLVQM
260 270 280 290 300
FIGDGAKLVR DAFALAKEKA PAIIFIDELD AIGTKRFDSE KAGDREVQRT
310 320 330 340 350
MLELLNQLDG FQPNDDIKVI AATNRIDVLD PALLRSGRLD RKIELPHPNE
360 370 380 390 400
DARARIMQIH SRKMNVNKDV NFEELARCTD DFNGAQCKAV CVEAGMIALR
410 420 430
RDATEILHED FMDAILEVQA KKKASLNYYA
Length:430
Mass (Da):48,126
Last modified:November 1, 1998 - v1
Checksum:i1B6DAA1E8E40E3EC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX284601 Genomic DNA. Translation: CCD62966.1.
PIRiT33155.
RefSeqiNP_491672.1. NM_059271.3.
UniGeneiCel.136.

Genome annotation databases

EnsemblMetazoaiF56H1.4; F56H1.4; WBGene00004505.
GeneIDi172238.
KEGGicel:CELE_F56H1.4.
UCSCiF56H1.4.2. c. elegans.

Similar proteinsi

Entry informationi

Entry nameiPRS6A_CAEEL
AccessioniPrimary (citable) accession number: O76371
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 22, 2017
Last sequence update: November 1, 1998
Last modified: November 22, 2017
This is version 150 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families