Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

O76290

- O76290_TRYBB

UniProt

O76290 - O76290_TRYBB

Protein
Submitted name:

Pteridine reductase

Gene

PTR1

Organism
Trypanosoma brucei brucei
Status
Unreviewed - Annotation score: 1 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 76 (01 Oct 2014)
      Sequence version 1 (01 Nov 1998)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei14 – 141Methotrexate
    Binding sitei14 – 141SubstrateImported
    Active sitei161 – 1611Proton donor/acceptorImported
    Active sitei174 – 1741Proton donor/acceptorImported
    Binding sitei174 – 1741Methotrexate
    Binding sitei174 – 1741NADPImported
    Binding sitei178 – 1781NADPImported
    Binding sitei217 – 2171Methotrexate
    Binding sitei221 – 2211Methotrexate

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi14 – 152NADPImported
    Nucleotide bindingi35 – 373NADPImported
    Nucleotide bindingi62 – 643NADPImported
    Nucleotide bindingi93 – 953NADPImported
    Nucleotide bindingi204 – 2085NADPImported

    GO - Molecular functioni

    1. nucleotide binding Source: UniProtKB-KW
    2. oxidoreductase activity Source: InterPro

    Keywords - Ligandi

    NADPImported, Nucleotide-bindingImported

    Names & Taxonomyi

    Protein namesi
    Submitted name:
    Pteridine reductaseImported
    Gene namesi
    Name:PTR1Imported
    OrganismiTrypanosoma brucei bruceiImported
    Taxonomic identifieri5702 [NCBI]
    Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeTrypanosoma

    Interactioni

    Protein-protein interaction databases

    STRINGi5691.Tb927.8.2210.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2C7VX-ray2.20A/B/C/D1-268[»]
    2VZ0X-ray1.90A/B/C/D1-268[»]
    2WD7X-ray1.90A/B/C/D1-268[»]
    2WD8X-ray2.10A/B/C/D1-268[»]
    2X9GX-ray1.10A/B/C/D1-268[»]
    2X9NX-ray1.15A/B/C/D1-268[»]
    2X9VX-ray1.30A/B/C/D1-268[»]
    2YHUX-ray2.01A/B/C/D1-268[»]
    3BMCX-ray2.60A/B/C/D1-268[»]
    3BMNX-ray1.98A/B/C/D1-268[»]
    3BMOX-ray1.60A/B/C/D1-268[»]
    3BMQX-ray1.70A/B/C/D1-268[»]
    3GN1X-ray2.00A/B/C/D1-268[»]
    3GN2X-ray1.60A/B/C/D1-268[»]
    3MCVX-ray1.70A/B/C/D1-268[»]
    ProteinModelPortaliO76290.
    SMRiO76290. Positions 2-268.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO76290.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni95 – 973Methotrexate binding

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR002198. DH_sc/Rdtase_SDR.
    IPR002347. Glc/ribitol_DH.
    IPR016040. NAD(P)-bd_dom.
    IPR014058. Pteridine_reductase.
    IPR020904. Sc_DH/Rdtase_CS.
    [Graphical view]
    PRINTSiPR00081. GDHRDH.
    PR00080. SDRFAMILY.
    TIGRFAMsiTIGR02685. pter_reduc_Leis. 1 hit.
    PROSITEiPS00061. ADH_SHORT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O76290-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEAPAAVVTG AAKRIGRAIA VKLHQTGYRV VIHYHNSAEA AVSLADELNK    50
    ERSNTAVVCQ ADLTNSNVLP ASCEEIINSC FRAFGRCDVL VNNASAFYPT 100
    PLVQGDHEDN SNGKTVETQV AELIGTNAIA PFLLTMSFAQ RQKGTNPNCT 150
    SSNLSIVNLC DAMVDQPCMA FSLYNMGKHA LVGLTQSAAL ELAPYGIRVN 200
    GVAPGVSLLP VAMGEEEKDK WRRKVPLGRR EASAEQIADA VIFLVSGSAQ 250
    YITGSIIKVD GGLSLVHA 268
    Length:268
    Mass (Da):28,470
    Last modified:November 1, 1998 - v1
    Checksum:i346C8257BDC9DD3D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF049903 mRNA. Translation: AAC23562.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF049903 mRNA. Translation: AAC23562.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2C7V X-ray 2.20 A/B/C/D 1-268 [» ]
    2VZ0 X-ray 1.90 A/B/C/D 1-268 [» ]
    2WD7 X-ray 1.90 A/B/C/D 1-268 [» ]
    2WD8 X-ray 2.10 A/B/C/D 1-268 [» ]
    2X9G X-ray 1.10 A/B/C/D 1-268 [» ]
    2X9N X-ray 1.15 A/B/C/D 1-268 [» ]
    2X9V X-ray 1.30 A/B/C/D 1-268 [» ]
    2YHU X-ray 2.01 A/B/C/D 1-268 [» ]
    3BMC X-ray 2.60 A/B/C/D 1-268 [» ]
    3BMN X-ray 1.98 A/B/C/D 1-268 [» ]
    3BMO X-ray 1.60 A/B/C/D 1-268 [» ]
    3BMQ X-ray 1.70 A/B/C/D 1-268 [» ]
    3GN1 X-ray 2.00 A/B/C/D 1-268 [» ]
    3GN2 X-ray 1.60 A/B/C/D 1-268 [» ]
    3MCV X-ray 1.70 A/B/C/D 1-268 [» ]
    ProteinModelPortali O76290.
    SMRi O76290. Positions 2-268.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 5691.Tb927.8.2210.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei O76290.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    InterProi IPR002198. DH_sc/Rdtase_SDR.
    IPR002347. Glc/ribitol_DH.
    IPR016040. NAD(P)-bd_dom.
    IPR014058. Pteridine_reductase.
    IPR020904. Sc_DH/Rdtase_CS.
    [Graphical view ]
    PRINTSi PR00081. GDHRDH.
    PR00080. SDRFAMILY.
    TIGRFAMsi TIGR02685. pter_reduc_Leis. 1 hit.
    PROSITEi PS00061. ADH_SHORT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Detke S.
      Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
    2. "Structure and reactivity of Trypanosoma brucei pteridine reductase: inhibition by the archetypal antifolate methotrexate."
      Dawson A., Gibellini F., Sienkiewicz N., Tulloch L.B., Fyfe P.K., McLuskey K., Fairlamb A.H., Hunter W.N.
      Mol. Microbiol. 61:1457-1468(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS) IN COMPLEX WITH NADP, ACTIVE SITE.
    3. "One scaffold, three binding modes: novel and selective pteridine reductase 1 inhibitors derived from fragment hits discovered by virtual screening."
      Mpamhanga C.P., Spinks D., Tulloch L.B., Shanks E.J., Robinson D.A., Collie I.T., Fairlamb A.H., Wyatt P.G., Frearson J.A., Hunter W.N., Gilbert I.H., Brenk R.
      J. Med. Chem. 52:4454-4465(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.60 ANGSTROMS) IN COMPLEX WITH NADP.
    4. "High-resolution structures of Trypanosoma brucei pteridine reductase ligand complexes inform on the placement of new molecular entities in the active site of a potential drug target."
      Dawson A., Tulloch L.B., Barrack K.L., Hunter W.N.
      Acta Crystallogr. D 66:1334-1340(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.10 ANGSTROMS) IN COMPLEX WITH NADP.
    5. "Development and validation of a cytochrome c-coupled assay for pteridine reductase 1 and dihydrofolate reductase."
      Shanks E.J., Ong H.B., Robinson D.A., Thompson S., Sienkiewicz N., Fairlamb A.H., Frearson J.A.
      Anal. Biochem. 396:194-203(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) IN COMPLEX WITH NADP.
    6. "Structure-based design of pteridine reductase inhibitors targeting African sleeping sickness and the leishmaniases."
      Tulloch L.B., Martini V.P., Iulek J., Huggan J.K., Lee J.H., Gibson C.L., Smith T.K., Suckling C.J., Hunter W.N.
      J. Med. Chem. 53:221-229(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.60 ANGSTROMS) IN COMPLEX WITH NADP.
    7. "Structural Studies of Thiadiazole Derivatives that Inhibit Trypanosoma Brucei Growth."
      Nerini E., Dawson A., Hannaert V., Michels P.A., Hunter W.N., Costi M.P.
      Submitted (MAY-2011) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (2.01 ANGSTROMS) IN COMPLEX WITH NADP.

    Entry informationi

    Entry nameiO76290_TRYBB
    AccessioniPrimary (citable) accession number: O76290
    Entry historyi
    Integrated into UniProtKB/TrEMBL: November 1, 1998
    Last sequence update: November 1, 1998
    Last modified: October 1, 2014
    This is version 76 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported

    External Data

    Dasty 3