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O76290 (O76290_TRYBB) Unreviewed, UniProtKB/TrEMBL

Last modified April 16, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein names
Gene names
Name:PTR1 EMBL AAC23562.1
OrganismTrypanosoma brucei brucei EMBL AAC23562.1
Taxonomic identifier5702 [NCBI]
Taxonomic lineageEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeTrypanosoma

Protein attributes

Sequence length268 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Nucleotide binding14 – 152NADP PDB 2C7V PDB 2VZ0 PDB 2WD7 PDB 2WD8 PDB 2YHU PDB 3BMC PDB 3BMN PDB 3BMO PDB 3BMQ PDB 2X9G PDB 2X9N PDB 2X9V PDB 3GN1 PDB 3MCV PDB 3GN2
Nucleotide binding35 – 373NADP PDB 2C7V PDB 2VZ0 PDB 2WD7 PDB 2WD8 PDB 2YHU PDB 3BMC PDB 3BMN PDB 3BMO PDB 3BMQ PDB 2X9G PDB 2X9N PDB 2X9V PDB 3GN1 PDB 3MCV PDB 3GN2
Nucleotide binding62 – 643NADP PDB 2C7V PDB 2VZ0 PDB 2WD7 PDB 2WD8 PDB 2YHU PDB 3BMC PDB 3BMN PDB 3BMO PDB 3BMQ PDB 2X9G PDB 2X9N PDB 2X9V PDB 3GN1 PDB 3MCV PDB 3GN2
Nucleotide binding93 – 953NADP PDB 2C7V PDB 2VZ0 PDB 2WD7 PDB 2WD8 PDB 2YHU PDB 3BMC PDB 3BMN PDB 3BMO PDB 3BMQ PDB 2X9G PDB 2X9N PDB 2X9V PDB 3GN1 PDB 3MCV PDB 3GN2
Nucleotide binding204 – 2085NADP PDB 2C7V PDB 2VZ0 PDB 2WD7 PDB 2WD8 PDB 2YHU PDB 3BMC PDB 3BMN PDB 3BMO PDB 3BMQ PDB 2X9G PDB 2X9N PDB 2X9V PDB 3GN1 PDB 3MCV PDB 3GN2
Region95 – 973Methotrexate binding

Sites

Active site1611Proton donor/acceptor
Active site1741Proton donor/acceptor
Binding site141Acetate 1; via amide nitrogen PDB 2X9N PDB 3GN1 PDB 3GN2
Binding site141Acetate 2; via amide nitrogen PDB 3BMO
Binding site141Chloride; via amide nitrogen PDB 2WD8
Binding site141Methotrexate
Binding site141Substrate
Binding site341Acetate 3 PDB 2YHU
Binding site341Acetate 4 PDB 3MCV
Binding site581Acetate 5; via amide nitrogen PDB 3BMO PDB 3BMQ PDB 2X9V PDB 3GN1
Binding site1011Acetate 6; via carbonyl oxygen PDB 2YHU
Binding site1691Acetate 7; via amide nitrogen PDB 3MCV
Binding site1741Methotrexate
Binding site1741NADP PDB 2WD8 PDB 2YHU PDB 3BMC PDB 3BMN PDB 3GN1 PDB 3MCV PDB 3GN2
Binding site1781NADP PDB 2C7V PDB 2VZ0 PDB 2WD7 PDB 2WD8 PDB 2YHU PDB 3BMC PDB 3BMN PDB 3BMO PDB 3BMQ PDB 2X9G PDB 2X9N PDB 2X9V PDB 3GN1 PDB 3MCV PDB 3GN2
Binding site1861Acetate 2 PDB 3BMO
Binding site1861Acetate 8 PDB 3BMN PDB 3BMO PDB 2X9V
Binding site2151Acetate 10; via amide nitrogen PDB 3BMQ PDB 2X9V PDB 3MCV
Binding site2151Acetate 12 PDB 3BMO
Binding site2151Acetate 9; via amide nitrogen PDB 3BMQ PDB 2X9G PDB 3MCV
Binding site2171Methotrexate
Binding site2211Methotrexate
Binding site2221Acetate 12 PDB 3BMO
Binding site2311Acetate 13 PDB 3MCV
Binding site2581Acetate 10 PDB 2X9V
Binding site2581Acetate 14 PDB 3BMN PDB 3BMO PDB 2X9V
Binding site2581Acetate 8 PDB 3BMO PDB 2X9V
Binding site2671Acetate 13 PDB 3MCV

Sequences

Sequence LengthMass (Da)Tools
O76290 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: 346C8257BDC9DD3D

FASTA26828,470
        10         20         30         40         50         60 
MEAPAAVVTG AAKRIGRAIA VKLHQTGYRV VIHYHNSAEA AVSLADELNK ERSNTAVVCQ 

        70         80         90        100        110        120 
ADLTNSNVLP ASCEEIINSC FRAFGRCDVL VNNASAFYPT PLVQGDHEDN SNGKTVETQV 

       130        140        150        160        170        180 
AELIGTNAIA PFLLTMSFAQ RQKGTNPNCT SSNLSIVNLC DAMVDQPCMA FSLYNMGKHA 

       190        200        210        220        230        240 
LVGLTQSAAL ELAPYGIRVN GVAPGVSLLP VAMGEEEKDK WRRKVPLGRR EASAEQIADA 

       250        260 
VIFLVSGSAQ YITGSIIKVD GGLSLVHA 

« Hide

References

[1]Detke S.
Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
[2]"Structure and reactivity of Trypanosoma brucei pteridine reductase: inhibition by the archetypal antifolate methotrexate."
Dawson A., Gibellini F., Sienkiewicz N., Tulloch L.B., Fyfe P.K., McLuskey K., Fairlamb A.H., Hunter W.N.
Mol. Microbiol. 61:1457-1468(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS) IN COMPLEX WITH METHOTREXATE AND NADP.
[3]"One scaffold, three binding modes: novel and selective pteridine reductase 1 inhibitors derived from fragment hits discovered by virtual screening."
Mpamhanga C.P., Spinks D., Tulloch L.B., Shanks E.J., Robinson D.A., Collie I.T., Fairlamb A.H., Wyatt P.G., Frearson J.A., Hunter W.N., Gilbert I.H., Brenk R.
J. Med. Chem. 52:4454-4465(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.60 ANGSTROMS) IN COMPLEX WITH ACETATE; CHLORIDE AND NADP.
[4]"High-resolution structures of Trypanosoma brucei pteridine reductase ligand complexes inform on the placement of new molecular entities in the active site of a potential drug target."
Dawson A., Tulloch L.B., Barrack K.L., Hunter W.N.
Acta Crystallogr. D 66:1334-1340(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.10 ANGSTROMS) IN COMPLEX WITH ACETATE AND NADP.
[5]"Development and validation of a cytochrome c-coupled assay for pteridine reductase 1 and dihydrofolate reductase."
Shanks E.J., Ong H.B., Robinson D.A., Thompson S., Sienkiewicz N., Fairlamb A.H., Frearson J.A.
Anal. Biochem. 396:194-203(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) IN COMPLEX WITH NADP.
[6]"Structure-based design of pteridine reductase inhibitors targeting African sleeping sickness and the leishmaniases."
Tulloch L.B., Martini V.P., Iulek J., Huggan J.K., Lee J.H., Gibson C.L., Smith T.K., Suckling C.J., Hunter W.N.
J. Med. Chem. 53:221-229(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.60 ANGSTROMS) IN COMPLEX WITH ACETATE AND NADP.
[7]"Structural Studies of Thiadiazole Derivatives that Inhibit Trypanosoma Brucei Growth."
Nerini E., Dawson A., Hannaert V., Michels P.A., Hunter W.N., Costi M.P.
Submitted (MAY-2011) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (2.01 ANGSTROMS) IN COMPLEX WITH ACETATE AND NADP.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF049903 mRNA. Translation: AAC23562.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2C7VX-ray2.20A/B/C/D1-268[»]
2VZ0X-ray1.90A/B/C/D1-268[»]
2WD7X-ray1.90A/B/C/D1-268[»]
2WD8X-ray2.10A/B/C/D1-268[»]
2X9GX-ray1.10A/B/C/D1-268[»]
2X9NX-ray1.15A/B/C/D1-268[»]
2X9VX-ray1.30A/B/C/D1-268[»]
2YHUX-ray2.01A/B/C/D1-268[»]
3BMCX-ray2.60A/B/C/D1-268[»]
3BMNX-ray1.98A/B/C/D1-268[»]
3BMOX-ray1.60A/B/C/D1-268[»]
3BMQX-ray1.70A/B/C/D1-268[»]
3GN1X-ray2.00A/B/C/D1-268[»]
3GN2X-ray1.60A/B/C/D1-268[»]
3MCVX-ray1.70A/B/C/D1-268[»]
ProteinModelPortalO76290.
SMRO76290. Positions 2-268.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING5691.Tb927.8.2210.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.50.720. 1 hit.
InterProIPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR014058. Pteridine_reductase.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]
PRINTSPR00081. GDHRDH.
PR00080. SDRFAMILY.
TIGRFAMsTIGR02685. pter_reduc_Leis. 1 hit.
PROSITEPS00061. ADH_SHORT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceO76290.

Entry information

Entry nameO76290_TRYBB
AccessionPrimary (citable) accession number: O76290
Entry history
Integrated into UniProtKB/TrEMBL: November 1, 1998
Last sequence update: November 1, 1998
Last modified: April 16, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)