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Protein

Alpha-amylase-related protein

Gene

Amyrel

Organism
Drosophila willistoni (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.By similarity

Cofactori

Protein has several cofactor binding sites:
  • Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity
  • chlorideBy similarityNote: Binds 1 Cl- ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi116 – 1161CalciumBy similarity
Metal bindingi167 – 1671Calcium; via carbonyl oxygenBy similarity
Metal bindingi176 – 1761CalciumBy similarity
Binding sitei204 – 2041ChlorideBy similarity
Active sitei206 – 2061NucleophileBy similarity
Metal bindingi210 – 2101Calcium; via carbonyl oxygenBy similarity
Active sitei243 – 2431Proton donorBy similarity
Binding sitei306 – 3061ChlorideBy similarity
Sitei308 – 3081Transition state stabilizerBy similarity
Binding sitei341 – 3411ChlorideBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

Calcium, Chloride, Metal-binding

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylase-related protein (EC:3.2.1.1By similarity)
Gene namesi
Name:Amyrel
OrganismiDrosophila willistoni (Fruit fly)
Taxonomic identifieri7260 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora

Organism-specific databases

FlyBaseiFBgn0025016. Dwil\Amyrel.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818By similarityAdd
BLAST
Chaini19 – 492474Alpha-amylase-related proteinPRO_0000001393Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei19 – 191Pyrrolidone carboxylic acidBy similarity
Disulfide bondi46 ↔ 102By similarity
Disulfide bondi155 ↔ 169By similarity
Disulfide bondi374 ↔ 380By similarity
Disulfide bondi416 ↔ 439Sequence analysis
Disulfide bondi446 ↔ 458By similarity

Keywords - PTMi

Disulfide bond, Pyrrolidone carboxylic acid

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi7260.FBpp0239873.

Structurei

3D structure databases

ProteinModelPortaliO76263.
SMRiO76263. Positions 19-492.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2212. Eukaryota.
COG0366. LUCA.
OrthoDBiEOG091G07V6.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006048. A-amylase/branching_C.
IPR031319. A-amylase_C.
IPR006046. Alpha_amylase.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
[Graphical view]
PRINTSiPR00110. ALPHAAMYLASE.
SMARTiSM00642. Aamy. 1 hit.
SM00632. Aamy_C. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O76263-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLSLSVLLC LGLALTLAQH NPHWWGNRNT IVHLFEWKWT DIADKCERFL
60 70 80 90 100
GPRGYAGVQV SPANENIISE GRPWWERYQP ISYKLVTRSG NELEFASMVK
110 120 130 140 150
RCNDVGVRIY VDVLLNHMSA DFEGLATGTG GSVAEPAKKS FPSVPYTADD
160 170 180 190 200
FHETCEITDW NDRFQVQQCE LVGLKDLNQN RDWVRTKLIE FLDHLIELGV
210 220 230 240 250
AGFRVDAAKH MASEDLSFIY SSVRDLNINH GFPNNARPFI YQEVIDHGHE
260 270 280 290 300
TVTREEYNEL GAVTEFRFSE EIGKAFRGNN ALKWLQSWGT DWGFLSSGQA
310 320 330 340 350
LTFVDNHDNQ RDSGDVLNYK SPKQYKMATA FHLAYPYGIS RVMSSFGFDD
360 370 380 390 400
RDQAPPQDAQ EQLISPEFDA DGGCTNGWIC EHRWRQIYNM VEFKNTVRDT
410 420 430 440 450
ELTNWWDNGD NQIAFCRGSK GFIAINNNLY NLSETLQTCL PAGEYCDVIS
460 470 480 490
GSLVDGACTG KSVSVDGNGY GYIHIGTEDF DGVLAIHTDA KL
Length:492
Mass (Da):55,641
Last modified:November 1, 1998 - v1
Checksum:iDFC711A762D8715D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF039560 Genomic DNA. Translation: AAC39095.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF039560 Genomic DNA. Translation: AAC39095.1.

3D structure databases

ProteinModelPortaliO76263.
SMRiO76263. Positions 19-492.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7260.FBpp0239873.

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

FlyBaseiFBgn0025016. Dwil\Amyrel.

Phylogenomic databases

eggNOGiKOG2212. Eukaryota.
COG0366. LUCA.
OrthoDBiEOG091G07V6.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006048. A-amylase/branching_C.
IPR031319. A-amylase_C.
IPR006046. Alpha_amylase.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
[Graphical view]
PRINTSiPR00110. ALPHAAMYLASE.
SMARTiSM00642. Aamy. 1 hit.
SM00632. Aamy_C. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAMYR_DROWI
AccessioniPrimary (citable) accession number: O76263
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: November 1, 1998
Last modified: September 7, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.