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Protein

Alpha-amylase-related protein

Gene

Amyrel

Organism
Drosophila sechellia (Fruit fly)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.By similarity

Cofactori

Protein has several cofactor binding sites:
  • Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity
  • chlorideBy similarityNote: Binds 1 Cl- ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi117CalciumBy similarity1
Metal bindingi168Calcium; via carbonyl oxygenBy similarity1
Metal bindingi177CalciumBy similarity1
Binding sitei205ChlorideBy similarity1
Active sitei207NucleophileBy similarity1
Metal bindingi211Calcium; via carbonyl oxygenBy similarity1
Active sitei244Proton donorBy similarity1
Binding sitei307ChlorideBy similarity1
Sitei309Transition state stabilizerBy similarity1
Binding sitei342ChlorideBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism
LigandCalcium, Chloride, Metal-binding

Protein family/group databases

CAZyiGH13 Glycoside Hydrolase Family 13

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylase-related protein (EC:3.2.1.1By similarity)
Gene namesi
Name:Amyrel
ORF Names:GM20043
OrganismiDrosophila sechellia (Fruit fly)
Taxonomic identifieri7238 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000001292 Componenti: Unassembled WGS sequence

Organism-specific databases

FlyBaseiFBgn0025060 Dsec\Amyrel

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19By similarityAdd BLAST19
ChainiPRO_000000138620 – 493Alpha-amylase-related proteinAdd BLAST474

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei20Pyrrolidone carboxylic acidBy similarity1
Disulfide bondi47 ↔ 103By similarity
Disulfide bondi156 ↔ 170By similarity
Disulfide bondi375 ↔ 381By similarity
Disulfide bondi417 ↔ 440Sequence analysis
Disulfide bondi447 ↔ 459By similarity

Keywords - PTMi

Disulfide bond, Pyrrolidone carboxylic acid

Proteomic databases

PRIDEiO76261

Interactioni

Subunit structurei

Monomer.By similarity

Structurei

3D structure databases

ProteinModelPortaliO76261
SMRiO76261
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

KOiK01176
OMAiQSWGTDW
OrthoDBiEOG091G07V6
PhylomeDBiO76261

Family and domain databases

Gene3Di2.60.40.1180, 1 hit
InterProiView protein in InterPro
IPR006048 A-amylase/branching_C
IPR031319 A-amylase_C
IPR006046 Alpha_amylase
IPR006047 Glyco_hydro_13_cat_dom
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
PfamiView protein in Pfam
PF00128 Alpha-amylase, 1 hit
PF02806 Alpha-amylase_C, 1 hit
PRINTSiPR00110 ALPHAAMYLASE
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SM00632 Aamy_C, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O76261-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFKFALTLTL CLAGSLSLAQ HNPHWWGNRN TIVHLFEWKW SDIAQECESF
60 70 80 90 100
LGPRGFAGVQ VSPVNENIIS AGRPWWERYQ PISYKLTTRS GNEEEFGDMV
110 120 130 140 150
RRCNDVGVRI YVDVLLNHMS GDFDGVAVGT AGTEAEPRKK SFPGVPYTAQ
160 170 180 190 200
DFHPTCEITD WNDRFQVQQC ELVGLKDLDQ SSDWVRSKLI EFLDHLIELG
210 220 230 240 250
VAGFRVDAAK HMASEDLEYI YSSLSNLNID HGFPHNSRPF IFQEVIDHGH
260 270 280 290 300
ETVSRDEYKD LGAVTEFRFS EEIGNAFRGN NALKWLQSWG TGWGFLPSGQ
310 320 330 340 350
ALTFVDNHDN QRDAGAVLSY KSPRQYKMAT AFHLAYPYGI SRVMSSFAFD
360 370 380 390 400
DHDTPPPQDA QERIISPEFD EDGACVNGWI CEHRWRQIYA MVGFKNAVRD
410 420 430 440 450
TEITGWWDNG DNQIAFCRGN KGFLAINNNL YDLSQDLNTC LPAGTYCDVI
460 470 480 490
SGSLIDGSCT GKSVTVNENG FGYIHIGSDD FDGVLALHVD AKV
Length:493
Mass (Da):55,461
Last modified:November 1, 1998 - v1
Checksum:i5E02E633FFB270EB
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti143P → T in strain: Seychelles-1 and Seychelles-15. 1 Publication1
Natural varianti152F → S in strain: Seychelles-12. 1 Publication1
Natural varianti293W → L in strain: Seychelles-7 and Seychelles-8. 1 Publication1
Natural varianti423F → S in strain: Seychelles-8 and Seychelles-10. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF039558 Genomic DNA Translation: AAC39093.1
EU018418 Genomic DNA Translation: ABW69388.1
EU018419 Genomic DNA Translation: ABW69389.1
EU018420 Genomic DNA Translation: ABW69390.1
EU018421 Genomic DNA Translation: ABW69391.1
EU018422 Genomic DNA Translation: ABW69392.1
EU018423 Genomic DNA Translation: ABW69393.1
EU018424 Genomic DNA Translation: ABW69394.1
EU018425 Genomic DNA Translation: ABW69395.1
EU018426 Genomic DNA Translation: ABW69396.1
EU018427 Genomic DNA Translation: ABW69397.1
EU018428 Genomic DNA Translation: ABW69398.1
EU018429 Genomic DNA Translation: ABW69399.1
EU018430 Genomic DNA Translation: ABW69400.1
EU018431 Genomic DNA Translation: ABW69401.1
EU018432 Genomic DNA Translation: ABW69402.1
CH480816 Genomic DNA Translation: EDW48174.1
RefSeqiXP_002034161.1, XM_002034125.1

Genome annotation databases

EnsemblMetazoaiFBtr0203028; FBpp0201520; FBgn0025060
GeneIDi6609476
KEGGidse:Dsec_GM20043

Similar proteinsi

Entry informationi

Entry nameiAMYR_DROSE
AccessioniPrimary (citable) accession number: O76261
Secondary accession number(s): B4HT73
, B6C8H3, B6C8H4, B6C8H9, B6C8I0, B6C8I2, B6C8I4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: November 1, 1998
Last modified: May 23, 2018
This is version 108 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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