O76242 (GLBN_CERLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Neural hemoglobin Short name=NrHb
Organism	Cerebratulus lacteus (Milky ribbon worm) (Micrura lactea)
Taxonomic identifier	6221 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Nemertea › Anopla › Heteronemertea › Cerebratulidae › Cerebratulus

Protein attributes

Sequence length	110 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as an oxygen store capable of sustaining neuronal activity in an anoxic environment for 5 to 30 min.
Subunit structure	Homotetramer. Self-associates in the deoxy state. Seems to dissociate upon oxygenation.
Sequence similarities	Belongs to the globin family.

Ontologies

Keywords
Biological process	Oxygen transport Transport
Ligand	Heme Iron Metal-binding
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Molecular function	heme binding Inferred from electronic annotation. Source: InterPro oxygen binding Inferred from electronic annotation. Source: InterPro oxygen transporter activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.1

Chain

2 – 110

109

Neural hemoglobin

PRO_0000052499

Sites

Metal binding

70

1

Iron (heme proximal ligand) By similarity

Secondary structure

1

.

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.

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.

.

.

.

110

Helix Strand Turn

Details...

Helix

4 – 18

15

Helix

20 – 25

6

Turn

27 – 30

4

Helix

33 – 38

6

Helix

40 – 58

19

Helix

63 – 71

9

Turn

72 – 74

3

Helix

77 – 93

17

Helix

100 – 107

8

Sequences

	Sequence		Length	Mass (Da)
	O76242 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 0F30C4420773F244	FASTA	110	11,530
	10 20 30 40 50 60 MVNWAAVVDD FYQELFKAHP EYQNKFGFKG VALGSLKGNA AYKTQAGKTV DYINAAIGGS 70 80 90 100 110 ADAAGLASRH KGRNVGSAEF HNAKACLAKA CSAHGAPDLG HAIDDILSHL

References

[1]	"The mini-hemoglobins in neural and body wall tissue of the nemertean worm, Cerebratulus lacteus." Vandergon T.L., Riggs C.K., Gorr T.A., Colacino J.M., Riggs A.F. J. Biol. Chem. 273:16998-17011(1998) [PubMed: 9642264] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-44, CHARACTERIZATION. Tissue: Brain.
[2]	"Crystallization and preliminary X-ray analysis of neural haemoglobin from the nemertean worm Cerebratulus lacteus." Pesce A., Nardini M., Dewilde S., Ascenzi P., Riggs A.F., Yamauchi K., Geuens E., Moens L., Bolognesi M. Acta Crystallogr. D 57:1897-1899(2001) [PubMed: 11717510] [Abstract] Cited for: CRYSTALLIZATION.
[3]	"The 109 residue nerve tissue minihemoglobin from Cerebratulus lacteus highlights striking structural plasticity of the alpha-helical globin fold." Pesce A., Nardini M., Dewilde S., Geuens E., Yamauchi K., Ascenzi P., Riggs A.F., Moens L., Bolognesi M. Structure 10:725-735(2002) [PubMed: 12015154] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF033001 Genomic DNA. Translation: AAC39125.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1KR7	X-ray	1.50	A	1-110	[»]
1V07	X-ray	1.70	A	2-109	[»]
2VYY	X-ray	1.60	A	1-110	[»]
2VYZ	X-ray	1.80	A	1-110	[»]
2XKG	X-ray	1.60	A	1-110	[»]
2XKH	X-ray	2.31	A	1-110	[»]
2XKI	X-ray	1.30	A	1-110	[»]

ProteinModelPortal

O76242.

SMR

O76242. Positions 1-110.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR009050. Globin-like.
IPR012292. Globin_dom.
IPR000971. Globin_subset.
[Graphical view]

Gene3D

G3DSA:1.10.490.10. Globin_related. 1 hit.

Pfam

PF00042. Globin. 1 hit.
[Graphical view]

SUPFAM

SSF46458. Globin_like. 1 hit.

PROSITE

PS01033. GLOBIN. False negative.
[Graphical view]

ProtoNet

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Entry information

Entry name

GLBN_CERLA

Accession

Primary (citable) accession number: O76242

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 10, 2002
Last sequence update:	January 23, 2007
Last modified:	October 19, 2011
This is version 62 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)