O76206 (RIFK_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative riboflavin kinase
EC=2.7.1.26

Alternative name(s):
ATP:riboflavin 5'-phosphotransferase
Flavokinase

Gene names

Name:	anon-84Ea
ORF Names:	CG2846

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	153 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin-mononucleotide (FMN) By similarity. UniProtKB Q969G6
Catalytic activity	ATP + riboflavin = ADP + FMN. UniProtKB Q969G6
Cofactor	Zinc or magnesium By similarity.
Pathway	Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (ATP route): step 1/1.
Subunit structure	Monomer By similarity. UniProtKB Q969G6
Subcellular location	Cytoplasm By similarity UniProtKB Q969G6.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding FMN Magnesium Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	FMN biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway riboflavin biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW riboflavin kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 153	153	Putative riboflavin kinase		PRO_0000194150
Sites
	Active site	80	1	Nucleophile By similarity
	Metal binding	28	1	Magnesium or zinc By similarity UniProtKB Q969G6
Experimental info
	Sequence conflict	24	1	L → M in AAL28446. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	O76206 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 56FF3B5C043C0D22	FASTA	153	16,986
	10 20 30 40 50 60 MLSQLPLFAG GEIVRGFGRG SKELGIPTAN FPLEVVKSLP ESLPTGAYYG WANVDNGPVH 70 80 90 100 110 120 KMVLSIGWNP FYNNKEKSVE THMLHDFNCD LYGQTLKICI VGYLRPERSF DSLESLIAAI 130 140 150 RGDIEQAKAF LDEADKAKLK EAPFFTEKLC SSK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Repetitive arrays containing a housekeeping gene promoter have altered polytene chromosome morphology in Drosophila." Clark D.V., Sabl J.F., Henikoff S. Chromosoma 107:96-104(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Canton-S.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo and Ovary.
[5]	Carlson J.W., Booth B., Frise E., Park S., Wan K.H., Yu C., Celniker S.E. Submitted (NOV-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF017096 Genomic DNA. Translation: AAC39087.1. AE014297 Genomic DNA. Translation: AAF54164.1. AY071393 mRNA. Translation: AAL49015.1. AY060898 mRNA. Translation: AAL28446.1. BT050515 mRNA. Translation: ACJ13222.1.
RefSeq	NP_649749.2. NM_141492.2.
UniGene	Dm.1095.
3D structure databases
ProteinModelPortal	O76206.
SMR	O76206. Positions 4-145.
ModBase	Search...
Protein-protein interaction databases
STRING	7227.FBpp0081260.
Proteomic databases
PaxDb	O76206.
PRIDE	O76206.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0081763; FBpp0081260; FBgn0014930.
GeneID	40936.
KEGG	dme:Dmel_CG2846.
Organism-specific databases
FlyBase	FBgn0014930. CG2846.
Phylogenomic databases
eggNOG	COG0196.
GeneTree	ENSGT00390000015537.
InParanoid	O76206.
KO	K00861.
OMA	KSMETHV.
OrthoDB	EOG4JWSX2.
PhylomeDB	O76206.
Enzyme and pathway databases
UniPathway	UPA00276; UER00406.
Gene expression databases
Bgee	O76206.
GermOnline	CG2846. Drosophila melanogaster.
Family and domain databases
Gene3D	2.40.30.30. 1 hit.
InterPro	IPR023468. Riboflavin_kinase. IPR015865. Riboflavin_kinase_bac/euk. IPR023465. Riboflavin_kinase_domain. [Graphical view]
PANTHER	PTHR22749. PTHR22749. 1 hit.
Pfam	PF01687. Flavokinase. 1 hit. [Graphical view]
SMART	SM00904. Flavokinase. 1 hit. [Graphical view]
SUPFAM	SSF82114. Riboflavin_kinase. 1 hit.
ProtoNet	Search...
Other
GenomeRNAi	40936.
NextBio	821367.

Entry information

Entry name

RIFK_DROME

Accession

Primary (citable) accession number: O76206
Secondary accession number(s): B6IDS5, Q95S97

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	November 1, 1998
Last modified:	April 3, 2013
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project