O76096 (CYTF_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cystatin-F

Alternative name(s):
Cystatin-7
Cystatin-like metastasis-associated protein
Short name=CMAP
Leukocystatin

Gene names

Name:

CST7

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	145 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Inhibits papain and cathepsin L but with affinities lower than other cystatins. May play a role in immune regulation through inhibition of a unique target in the hematopoietic system.
Subunit structure	Homodimer; disulfide-linked. Ref.10
Subcellular location	Secreted. Cytoplasm Ref.4.
Tissue specificity	Primarily expressed in peripheral blood cells and spleen.
Sequence similarities	Belongs to the cystatin family.
Sequence caution	The sequence AAH15507.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence AAP88827.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence BAA34941.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence BAB11886.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence CAB75498.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Cellular component	Cytoplasm Secreted
Domain	Signal
Molecular function	Protease inhibitor Thiol protease inhibitor
PTM	Disulfide bond Glycoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	immune response Traceable author statement. Source: ProtInc
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cysteine-type endopeptidase inhibitor activity Traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 19

19

Potential

Chain

20 – 145

126

Cystatin-F

PRO_0000006646

Regions

Motif

81 – 85

5

Secondary area of contact

Sites

Site

37

1

Reactive site

Amino acid modifications

Glycosylation

62

1

N-linked (GlcNAc...) Ref.10

Glycosylation

115

1

N-linked (GlcNAc...) Ref.10

Disulfide bond

26

Interchain (with C-63) Ref.10

Disulfide bond

63

Interchain (with C-26) Ref.10

Disulfide bond

99 ↔ 110

Ref.10

Disulfide bond

124 ↔ 144

Ref.10

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

145

Helix Strand Turn

Details...

Beta strand

28 – 34

7

Helix

47 – 63

17

Beta strand

67 – 100

34

Helix

107 – 109

3

Turn

116 – 118

3

Beta strand

121 – 131

11

Helix

132 – 134

3

Sequences

	Sequence		Length	Mass (Da)
	O76096 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: B2BCC4F76857CB0F	FASTA	145	16,454
	10 20 30 40 50 60 MRAAGTLLAF CCLVLSTTGG PSPDTCSQDL NSRVKPGFPK TIKTNDPGVL QAARYSVEKF 70 80 90 100 110 120 NNCTNDMFLF KESRITRALV QIVKGLKYML EVEIGRTTCK KNQHLRLDDC DFQTNHTLKQ 130 140 TLSCYSEVWV VPWLQHFEVP VLRCH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Leukocystatin, a new class II cystatin expressed selectively by hematopoietic cells." Halfon S., Ford J., Foster J., Dowling L., Lucian L., Sterling M., Xu Y., Weiss M., Ikeda M., Liggett D., Helms A., Caux C., Lebecque S., Hannum C., Menon S., McClanahan T., Gorman D., Zurawski G. J. Biol. Chem. 273:16400-16408(1998) [PubMed: 9632704] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Cystatin F is a glycosylated human low molecular weight cysteine proteinase inhibitor." Ni J., Fernandez M.A., Danielsson L., Chillakuru R.A., Zhang J., Grubb A., Su J., Gentz R., Abrahamson M. J. Biol. Chem. 273:24797-24804(1998) [PubMed: 9733783] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Genomic construct and mapping of the gene for CMAP (leukocystatin/cystatin F, CST7) and identification of a proximal novel gene, BSCv (C20orf3)." Morita M., Hara Y., Tamai Y., Arakawa H., Nishimura S. Genomics 67:87-91(2000) [PubMed: 10945474] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Regulated expression and intracellular localization of cystatin F in human U937 cells." Nathanson C.M., Wasselius J., Wallin H., Abrahamson M. Eur. J. Biochem. 269:5502-5511(2002) [PubMed: 12423348] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION. Tissue: Blood.
[5]	"Human homologue of murine CMAP." Morita M., Arakawa H., Yoshiuchi N. Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[6]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[7]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[8]	"The DNA sequence and comparative analysis of human chromosome 20." Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J. Nature 414:865-871(2001) [PubMed: 11780052] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[9]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
[10]	"Structural basis of reduction-dependent activation of human cystatin F." Schuettelkopf A.W., Hamilton G., Watts C., van Aalten D.M.F. J. Biol. Chem. 281:16570-16575(2006) [PubMed: 16601115] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 20-145, SUBUNIT, GLYCOSYLATION AT ASN-62 AND ASN-115, DISULFIDE BONDS.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF031824 mRNA. Translation: AAC39788.1.
AF036342 mRNA. Translation: AAC35747.1.
AB029636 Genomic DNA. Translation: BAB11886.1. Different initiation.
AJ510167 , AJ510168, AJ510169, AJ510170 Genomic DNA. Translation: CAD52872.1.
AB015225 mRNA. Translation: BAA34941.1. Different initiation.
BT009825 mRNA. Translation: AAP88827.1. Different initiation.
CR541860 mRNA. Translation: CAG46658.1.
CR541878 mRNA. Translation: CAG46676.1.
AL035661 Genomic DNA. Translation: CAB75498.2. Different initiation.
BC015507 mRNA. Translation: AAH15507.1. Different initiation.

IPI

IPI00216569.

RefSeq

NP_003641.3. NM_003650.3.

UniGene

Hs.143212.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2CH9	X-ray	2.10	A	20-145	[»]

ProteinModelPortal

O76096.

SMR

O76096. Positions 25-144.

ModBase

Search...

Protein-protein interaction databases

IntAct

O76096. 1 interaction.

MINT

MINT-6179682.

STRING

O76096.

Protein family/group databases

MEROPS

I25.007.

Proteomic databases

PRIDE

O76096.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000376835; ENSP00000366031; ENSG00000077984.
ENST00000480798; ENSP00000420384; ENSG00000077984.

GeneID

8530.

KEGG

hsa:8530.

UCSC

uc002wtx.1. human.

Organism-specific databases

CTD

8530.

GeneCards

GC20P024924.

HGNC

HGNC:2479. CST7.

MIM

603253. gene.

neXtProt

NX_O76096.

PharmGKB

PA26980.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG16003.

GeneTree

ENSGT00600000084460.

HOGENOM

HBG717109.

HOVERGEN

HBG009556.

InParanoid

O76096.

OrthoDB

EOG4KH2WN.

Gene expression databases

ArrayExpress

O76096.

Bgee

O76096.

CleanEx

HS_CST7.

Genevestigator

O76096.

GermOnline

ENSG00000077984. Homo sapiens.

Family and domain databases

InterPro

IPR000010. Prot_inh_cystat.
[Graphical view]

KO

K13903.

Pfam

PF00031. Cystatin. 1 hit.
[Graphical view]

SMART

SM00043. CY. 1 hit.
[Graphical view]

PROSITE

PS00287. CYSTATIN. False negative.
[Graphical view]

ProtoNet

Search...

Other

NextBio

31944.

SOURCE

Search...

Entry information

Entry name

CYTF_HUMAN

Accession

Primary (citable) accession number: O76096
Secondary accession number(s): Q6FH95, Q7Z4J8, Q9UED4

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1998
Last modified:	November 16, 2011
This is version 102 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.