O76095 (JTB_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein JTB

Alternative name(s):
Jumping translocation breakpoint protein
Prostate androgen-regulated protein
Short name=PAR protein

Gene names

Name:	JTB
ORF Names:	HSPC222

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	146 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for normal cytokinesis during mitosis. Plays a role in the regulation of cell proliferation. May be a component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Increases AURKB activity. Inhibits apoptosis induced by TGFB1 By similarity. Overexpression induces swelling of mitochondria and reduces mitochondrial membrane potential By similarity. Ref.12
Subunit structure	Interacts with AURKA, AURKB, BIRC5 and INCENP. May be a component of the CPC at least composed of BIRC5/survivin, CDCA8/borealin, INCENP and AURKB/Aurora-B. Ref.12
Subcellular location	Membrane; Single-pass type I membrane protein Potential. Mitochondrion By similarity. Cytoplasm. Cytoplasm › cytoskeleton › centrosome. Cytoplasm › cytoskeleton › spindle. Note: Detected at the centrosome and along spindle fibers during prophase and metaphase. Detected at the midbody during telophase. Ref.1 Ref.12
Tissue specificity	Ubiquitous. Expressed in all normal human tissues studied but overexpressed or underexpressed in many of their malignant counterparts. Ref.1 Ref.2 Ref.11 Ref.12
Induction	Protein levels increase during the S phase of the cell cycle, are highest during G2 and mitosis, and decrease to low levels at G1. Levels are lowest at the transition from G1 to S phase. Ref.12
Sequence similarities	Belongs to the JTB family.

Ontologies

Keywords
Biological process	Apoptosis Cell cycle Cell division Mitosis
Cellular component	Cytoplasm Cytoskeleton Membrane Mitochondrion
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Signal Transmembrane Transmembrane helix
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW cell cycle cytokinesis Inferred from mutant phenotype Ref.12. Source: UniProtKB mitosis Inferred from mutant phenotype Ref.12. Source: UniProtKB negative regulation of apoptotic process Inferred from electronic annotation. Source: Compara positive regulation of protein kinase activity Inferred from mutant phenotype Ref.12. Source: UniProtKB regulation of cell proliferation Inferred from electronic annotation. Source: Compara
Cellular_component	cytoplasm Inferred from direct assay Ref.12. Source: UniProtKB integral to plasma membrane Traceable author statement Ref.1. Source: ProtInc microtubule organizing center Inferred from electronic annotation. Source: UniProtKB-SubCell midbody Inferred from direct assay Ref.12. Source: UniProtKB mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell spindle Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	protein kinase binding Inferred from direct assay Ref.12. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O76095-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O76095-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-38: MLAGAGRPGLPQGRHLCWLLCAFTLKLCQAEAPVQEEK → MAWGWHLSF

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 30

30

Potential

Chain

31 – 146

116

Protein JTB

PRO_0000021535

Regions

Topological domain

31 – 105

75

Extracellular Potential

Transmembrane

106 – 126

21

Helical; Potential

Topological domain

127 – 146

20

Cytoplasmic Potential

Natural variations

Alternative sequence

1 – 38

38

MLAGA…VQEEK → MAWGWHLSF in isoform 2.

VSP_041400

Natural variant

16

1

L → F.
Corresponds to variant rs34686244 [ dbSNP | Ensembl ].

VAR_033994

Secondary structure

1

.

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.

.

.

.

.

.

.

.

.

.

.

.

.

.

146

Helix Strand Turn

Details...

Turn

49 – 51

3

Beta strand

54 – 62

9

Helix

65 – 70

6

Helix

72 – 74

3

Turn

75 – 77

3

Beta strand

79 – 85

7

Turn

86 – 89

4

Beta strand

90 – 95

6

Helix

98 – 101

4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: CA7080543591C319	FASTA	146	16,358
	10 20 30 40 50 60 MLAGAGRPGL PQGRHLCWLL CAFTLKLCQA EAPVQEEKLS ASTSNLPCWL VEEFVVAEEC 70 80 90 100 110 120 SPCSNFRAKT TPECGPTGYV EKITCSSSKR NEFKSCRSAL MEQRLFWKFE GAVVCVALIF 130 140 ACLVIIRQRQ LDRKALEKVR KQIESI
	Isoform 2 [UniParc]. Checksum: 39BB62469FA5541B	FASTA	117	13,356
	10 20 30 40 50 60 MAWGWHLSFL SASTSNLPCW LVEEFVVAEE CSPCSNFRAK TTPECGPTGY VEKITCSSSK 70 80 90 100 110 RNEFKSCRSA LMEQRLFWKF EGAVVCVALI FACLVIIRQR QLDRKALEKV RKQIESI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"JTB: a novel membrane protein gene at 1q21 rearranged in a jumping translocation." Hatakeyama S., Osawa M., Omine M., Ishikawa F. Oncogene 18:2085-2090(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[2]	"PAR, a novel androgen regulated gene, ubiquitously expressed in normal and malignant cells." Platica O., Chen S., Ivan E., Lopingco M.C., Holland J.F., Platica M. Int. J. Oncol. 16:1055-1061(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY. Tissue: Mammary cancer and Prostatic carcinoma.
[3]	Mei G., Yu W., Gibbs R.A. Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[4]	"Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells." Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G., Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W., Tao J., Huang Q.-H., Zhou J., Hu G.-X. , Gu J., Chen S.-J., Chen Z. Genome Res. 10:1546-1560(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Umbilical cord blood.
[5]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[6]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[7]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Tongue.
[8]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[9]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[10]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Cervix, Lung, Skin and Uterus.
[11]	"Characterization of Jumping translocation breakpoint (JTB) gene product isolated as a TGF-beta1-inducible clone involved in regulation of mitochondrial function, cell growth and cell death." Kanome T., Itoh N., Ishikawa F., Mori K., Kim-Kaneyama J.R., Nose K., Shibanuma M. Oncogene 26:5991-6001(2007) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[12]	"PAR, a protein involved in the cell cycle, is functionally related to chromosomal passenger proteins." Platica M., Ionescu A., Ivan E., Holland J.F., Mandeli J., Platica O. Int. J. Oncol. 38:777-785(2011) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH AURKA; AURKB AND BIRC5, SUBUNIT, INDUCTION, TISSUE SPECIFICITY.
[13]	"The structure of the extracellular domain of the jumping translocation breakpoint protein reveals a variation of the midkine fold." Rousseau F., Pan B., Fairbrother W.J., Bazan J.F., Lingel A. J. Mol. Biol. 415:22-28(2012) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR OF 47-104.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AB016492 Genomic DNA. Translation: BAA33735.1.
AB016488 mRNA. Translation: BAA33731.1.
AB016493 Genomic DNA. Translation: BAA33736.1.
AF115850 mRNA. Translation: AAD09822.2.
AF131797 mRNA. Translation: AAD20045.1.
AF151056 mRNA. Translation: AAF36142.1.
BT007285 mRNA. Translation: AAP35949.1.
CR456985 mRNA. Translation: CAG33266.1.
AK312106 mRNA. Translation: BAG35042.1.
AL358472 Genomic DNA. Translation: CAI14028.1.
AL358472 Genomic DNA. Translation: CAI14029.1.
AL358472 Genomic DNA. Translation: CAI14030.1.
CH471121 Genomic DNA. Translation: EAW53241.1.
CH471121 Genomic DNA. Translation: EAW53242.1.
CH471121 Genomic DNA. Translation: EAW53244.1.
BC000499 mRNA. Translation: AAH00499.1.
BC000996 mRNA. Translation: AAH00996.1.
BC001363 mRNA. Translation: AAH01363.1.
BC001667 mRNA. Translation: AAH01667.1.
BC004239 mRNA. Translation: AAH04239.1.
BC019277 mRNA. Translation: AAH19277.1.

IPI

IPI00000912.
IPI00008833.

RefSeq

NP_006685.1. NM_006694.3.

UniGene

Hs.6396.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2KJX	NMR	-	A	47-104	[»]

ProteinModelPortal

O76095.

ModBase

Search...

Protein-protein interaction databases

IntAct

O76095. 5 interactions.

STRING

9606.ENSP00000271843.

PTM databases

PhosphoSite

O76095.

Proteomic databases

PaxDb

O76095.

PRIDE

O76095.

Protocols and materials databases

DNASU

10899.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000271843; ENSP00000271843; ENSG00000143543.
ENST00000356648; ENSP00000349069; ENSG00000143543.
ENST00000368589; ENSP00000357578; ENSG00000143543.
ENST00000428469; ENSP00000395250; ENSG00000143543.

GeneID

10899.

KEGG

hsa:10899.

UCSC

uc001fds.3. human.

Organism-specific databases

CTD

10899.

GeneCards

GC01M153946.

HGNC

HGNC:6201. JTB.

HPA

HPA006514.

MIM

604671. gene.

neXtProt

NX_O76095.

PharmGKB

PA30003.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG296098.

HOGENOM

HOG000294204.

HOVERGEN

HBG000952.

InParanoid

O76095.

OMA

TPECGST.

OrthoDB

EOG49S67M.

PhylomeDB

O76095.

Gene expression databases

Bgee

O76095.

CleanEx

HS_JTB.

Genevestigator

O76095.

GermOnline

ENSG00000143543. Homo sapiens.

Family and domain databases

InterPro

IPR008657. JTB.
[Graphical view]

PANTHER

PTHR13041. PTHR13041. 1 hit.

Pfam

PF05439. JTB. 1 hit.
[Graphical view]

ProDom

PD151382. JTB. 1 hit.
[Graphical view] [Entries sharing at least one domain]

ProtoNet

Search...

Other

EvolutionaryTrace

O76095.

GenomeRNAi

10899.

NextBio

41391.

SOURCE

Search...

Entry information

Entry name

JTB_HUMAN

Accession

Primary (citable) accession number: O76095
Secondary accession number(s): O95442, Q6IB19, Q9P0Q4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	November 1, 1998
Last modified:	May 1, 2013
This is version 102 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.