O76094 (SRP72_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Signal recognition particle subunit SRP72
Short name=SRP72

Alternative name(s):
Signal recognition particle 72 kDa protein

Gene names

Name:

SRP72

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	671 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Signal-recognition-particle assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum membrane. Binds the 7S RNA only in presence of SRP68. This ribonucleoprotein complex might interact directly with the docking protein in the ER membrane and possibly participate in the elongation arrest function.
Subunit structure	Signal recognition particle consists of a 7S RNA molecule of 300 nucleotides and six protein subunits: SRP72, SRP68, SRP54, SRP19, SRP14 and SRP9.
Subcellular location	Cytoplasm. Endoplasmic reticulum Ref.11.
Domain	The C-terminus is essential for the interaction with the SRP68/7S RNA complex By similarity.
Involvement in disease	Bone marrow failure, familial (BMFF) [MIM:614675]: An autosomal dominant disease characterized by aplastic anemia and myelodysplasia resulting from bone marrow failure. Aplastic anemia is a form of anemia in which the bone marrow fails to produce adequate numbers of peripheral blood elements. Myelodysplasia is a clonal hematopoietic stem cell disorder in which immature cells in the bone marrow become malformed and dysfunctional. Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.11
Sequence similarities	Belongs to the SRP72 family. Contains 5 TPR repeats.
Sequence caution	The sequence CAD97950.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence CAD97950.1 differs from that shown. Reason: Frameshift at position 565.

Ontologies

Keywords
Cellular component	Cytoplasm Endoplasmic reticulum Signal recognition particle
Coding sequence diversity	Alternative splicing
Disease	Disease mutation
Domain	Repeat TPR repeat
Molecular function	Ribonucleoprotein
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	SRP-dependent cotranslational protein targeting to membrane Traceable author statement. Source: Reactome response to drug Inferred from direct assay PubMed 18089836. Source: UniProtKB translation Traceable author statement. Source: Reactome
Cellular_component	cytosol Traceable author statement. Source: Reactome endoplasmic reticulum Inferred from direct assay Ref.11. Source: UniProtKB nucleolus Inferred from direct assay. Source: HPA plasma membrane Inferred from direct assay. Source: HPA signal recognition particle, endoplasmic reticulum targeting Inferred from direct assay PubMed 18089836. Source: UniProtKB
Molecular_function	7S RNA binding Inferred from electronic annotation. Source: InterPro signal recognition particle binding Inferred from physical interaction PubMed 17254600. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O76094-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O76094-2)

The sequence of this isoform differs from the canonical sequence as follows:
     215-275: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.6
	Chain	2 – 671	670	Signal recognition particle subunit SRP72		PRO_0000135234
Regions
	Repeat	11 – 44	34	TPR 1
	Repeat	109 – 142	34	TPR 2
	Repeat	226 – 259	34	TPR 3
	Repeat	406 – 439	34	TPR 4
	Repeat	447 – 480	34	TPR 5
	Compositional bias	552 – 561	10	Poly-Lys
	Compositional bias	662 – 665	4	Poly-Lys
Amino acid modifications
	Modified residue	2	1	N-acetylalanine Ref.6
	Modified residue	571	1	Phosphothreonine Ref.7
	Modified residue	618	1	Phosphothreonine Ref.8 Ref.9
Natural variations
	Alternative sequence	215 – 275	61	Missing in isoform 2.		VSP_045576
	Natural variant	207	1	R → H in BMFF; affects protein localization to ER. Ref.11		VAR_068522
Experimental info
	Sequence conflict	23	1	Q → R in CAD97950. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: CC0BA17AC52E3613	FASTA	671	74,606
	10 20 30 40 50 60 MASGGSGGVS VPALWSEVNR YGQNGDFTRA LKTVNKILQI NKDDVTALHC KVVCLIQNGS 70 80 90 100 110 120 FKEALNVINT HTKVLANNSL SFEKAYCEYR LNRIENALKT IESANQQTDK LKELYGQVLY 130 140 150 160 170 180 RLERYDECLA VYRDLVRNSQ DDYDEERKTN LSAVVAAQSN WEKVVPENLG LQEGTHELCY 190 200 210 220 230 240 NTACALIGQG QLNQAMKILQ KAEDLCRRSL SEDTDGTEED PQAELAIIHG QMAYILQLQG 250 260 270 280 290 300 RTEEALQLYN QIIKLKPTDV GLLAVIANNI ITINKDQNVF DSKKKVKLTN AEGVEFKLSK 310 320 330 340 350 360 KQLQAIEFNK ALLAMYTNQA EQCRKISASL QSQSPEHLLP VLIQAAQLCR EKQHTKAIEL 370 380 390 400 410 420 LQEFSDQHPE NAAEIKLTMA QLKISQGNIS KACLILRSIE ELKHKPGMVS ALVTMYSHEE 430 440 450 460 470 480 DIDSAIEVFT QAIQWYQNHQ PKSPAHLSLI REAANFKLKY GRKKEAISDL QQLWKQNPKD 490 500 510 520 530 540 IHTLAQLISA YSLVDPEKAK ALSKHLPSSD SMSLKVDVEA LENSAGATYI RKKGGKVTGD 550 560 570 580 590 600 SQPKEQGQGD LKKKKKKKKG KLPKNYDPKV TPDPERWLPM RERSYYRGRK KGKKKDQIGK 610 620 630 640 650 660 GTQGATAGAS SELDASKTVS SPPTSPRPGS AATVSASTSN IIPPRHQKPA GAPATKKKQQ 670 QKKKKGGKGG W
	Isoform 2 [UniParc]. Checksum: 6351C7451A38ECEC	FASTA	610	67,879
	10 20 30 40 50 60 MASGGSGGVS VPALWSEVNR YGQNGDFTRA LKTVNKILQI NKDDVTALHC KVVCLIQNGS 70 80 90 100 110 120 FKEALNVINT HTKVLANNSL SFEKAYCEYR LNRIENALKT IESANQQTDK LKELYGQVLY 130 140 150 160 170 180 RLERYDECLA VYRDLVRNSQ DDYDEERKTN LSAVVAAQSN WEKVVPENLG LQEGTHELCY 190 200 210 220 230 240 NTACALIGQG QLNQAMKILQ KAEDLCRRSL SEDTDQNVFD SKKKVKLTNA EGVEFKLSKK 250 260 270 280 290 300 QLQAIEFNKA LLAMYTNQAE QCRKISASLQ SQSPEHLLPV LIQAAQLCRE KQHTKAIELL 310 320 330 340 350 360 QEFSDQHPEN AAEIKLTMAQ LKISQGNISK ACLILRSIEE LKHKPGMVSA LVTMYSHEED 370 380 390 400 410 420 IDSAIEVFTQ AIQWYQNHQP KSPAHLSLIR EAANFKLKYG RKKEAISDLQ QLWKQNPKDI 430 440 450 460 470 480 HTLAQLISAY SLVDPEKAKA LSKHLPSSDS MSLKVDVEAL ENSAGATYIR KKGGKVTGDS 490 500 510 520 530 540 QPKEQGQGDL KKKKKKKKGK LPKNYDPKVT PDPERWLPMR ERSYYRGRKK GKKKDQIGKG 550 560 570 580 590 600 TQGATAGASS ELDASKTVSS PPTSPRPGSA ATVSASTSNI IPPRHQKPAG APATKKKQQQ 610 KKKKGGKGGW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Protein SRP72 sequence of human signal recognition particle." Gowda K., Zwieb C. Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Sequence of human signal recognition particle (SRP) 72." Utz P.J., Hottelet M., Miller I.J., Anderson P. Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Esophageal carcinoma.
[4]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	Bienvenut W.V., Waridel P., Quadroni M. Submitted (MAR-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-20; 63-84; 113-121; 148-163; 242-275; 288-297; 302-324; 326-350; 357-376; 465-475; 480-498; 516-531 AND 601-617, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-571, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-618, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[9]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-618, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[10]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]	"Exome sequencing identifies autosomal-dominant SRP72 mutations associated with familial aplasia and myelodysplasia." Kirwan M., Walne A.J., Plagnol V., Velangi M., Ho A., Hossain U., Vulliamy T., Dokal I. Am. J. Hum. Genet. 90:888-892(2012) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT BMFF HIS-207, SUBCELLULAR LOCATION, CHARACTERIZATION OF VARIANT BMFF HIS-207.
+	Additional computationally mapped references.

Web resources

Wikipedia

Signal recognition particle entry

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF077019 mRNA. Translation: AAC27324.1. AF069765 mRNA. Translation: AAC97490.1. BX537991 mRNA. Translation: CAD97950.1. Sequence problems. AC114766 Genomic DNA. No translation available. CH471057 Genomic DNA. Translation: EAX05498.1.
IPI	IPI00215888. IPI00966751.
RefSeq	NP_001254651.1. NM_001267722.1. NP_008878.3. NM_006947.3.
UniGene	Hs.237825.
3D structure databases
ProteinModelPortal	O76094.
ModBase	Search...
Protein-protein interaction databases
IntAct	O76094. 5 interactions.
MINT	MINT-1197309.
STRING	9606.ENSP00000342181.
Protein family/group databases
TCDB	3.A.5.9.1. general secretory pathway (Sec) family.
PTM databases
PhosphoSite	O76094.
Proteomic databases
PaxDb	O76094.
PeptideAtlas	O76094.
PRIDE	O76094.
Protocols and materials databases
DNASU	6731.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000342756; ENSP00000342181; ENSG00000174780. ENST00000510663; ENSP00000424576; ENSG00000174780.
GeneID	6731.
KEGG	hsa:6731.
UCSC	uc003hbv.3. human.
Organism-specific databases
CTD	6731.
GeneCards	GC04P057334.
HGNC	HGNC:11303. SRP72.
HPA	HPA034621.
MIM	602122. gene. 614675. phenotype.
neXtProt	NX_O76094.
PharmGKB	PA36127.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG263236.
HOGENOM	HOG000006745.
HOVERGEN	HBG059920.
InParanoid	O76094.
KO	K03108.
OMA	ATYVRKK.
OrthoDB	EOG42Z4PV.
Enzyme and pathway databases
Reactome	REACT_17015. Metabolism of proteins. REACT_71. Gene Expression.
Gene expression databases
ArrayExpress	O76094.
Bgee	O76094.
CleanEx	HS_SRP72.
Genevestigator	O76094.
GermOnline	ENSG00000174780. Homo sapiens.
Family and domain databases
Gene3D	1.25.40.10. 4 hits.
InterPro	IPR013699. Signal_recog_part_SRP72_RNA-bd. IPR026270. SRP72. IPR001440. TPR-1. IPR013026. TPR-contain_dom. IPR011990. TPR-like_helical. IPR019734. TPR_repeat. [Graphical view]
PANTHER	PTHR14094. PTHR14094. 1 hit.
Pfam	PF08492. SRP72. 1 hit. PF00515. TPR_1. 1 hit. PF13174. TPR_6. 1 hit. [Graphical view]
PIRSF	PIRSF038922. SRP72. 1 hit.
PROSITE	PS50005. TPR. 5 hits. PS50293. TPR_REGION. 3 hits. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	SRP72. human.
GenomeRNAi	6731.
NextBio	26258.
SOURCE	Search...

Entry information

Entry name

SRP72_HUMAN

Accession

Primary (citable) accession number: O76094
Secondary accession number(s): G5E9Z8, Q7Z3C0

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 110 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.