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O76081 (RGS20_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 131. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Regulator of G-protein signaling 20

Short name=RGS20
Alternative name(s):
Gz-selective GTPase-activating protein
Short name=G(z)GAP
Short name=Gz-GAP
Regulator of G-protein signaling Z1
Regulator of Gz-selective protein signaling 1
Gene names
Name:RGS20
Synonyms:RGSZ1, ZGAP1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length388 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds selectively to G(z)-alpha and G(alpha)-i2 subunits, accelerates their GTPase activity and regulates their signaling activities. The G(z)-alpha activity is inhibited by the phosphorylation and palmitoylation of the G-protein. Negatively regulates mu-opioid receptor-mediated activation of the G-proteins By similarity. Ref.7

Subunit structure

Forms a complex with G(alpha)z/i2 subunits and mu-opioid receptors; the formation of this complex results in mu-opioid receptor desensitization. Interacts with OPRM1 By similarity.

Subcellular location

Membrane; Lipid-anchor. Nucleus. Cytoplasm. Note: Shuttles between the cytoplasm/cell membrane and the nucleus By similarity. Anchored to the membrane through palmitoylation By similarity.

Tissue specificity

Isoform 5 is expressed in brain at high levels in the caudate nucleus and temporal lobe.

Post-translational modification

Fatty acylated. Heavily palmitoylated in the cysteine string motif By similarity.

N- and O-glycosylated in synapsomal membranes By similarity.

Serine phosphorylated in synapsomal membranes By similarity.

Sumoylated with SUMO1 and SUMO2 in synaptosomes. The sumoylated forms act as a scaffold for sequestering mu-opioid receptor-activated G(alpha) subunits By similarity.

Sequence similarities

Contains 1 RGS domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Hoxa1P090223EBI-1052678,EBI-3957603From a different organism.
MTNR1AP480396EBI-1052678,EBI-1188238
MTNR1BP492862EBI-1052678,EBI-1188341

Alternative products

This entry describes 6 isoforms produced by alternative splicing. [Align] [Select]
Isoform 6 (identifier: O76081-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 1 (identifier: O76081-2)

The sequence of this isoform differs from the canonical sequence as follows:
     56-170: Missing.
Isoform 2 (identifier: O76081-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-208: Missing.
     209-220: CFCWCCCCSCSC → MKETSGLFLISS
Isoform 3 (identifier: O76081-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-236: Missing.
     237-247: ELRADLPTWEE → MKETSGLFLIS
Isoform 4 (identifier: O76081-5)

The sequence of this isoform differs from the canonical sequence as follows:
     3-55: Missing.
     56-170: Missing.
Isoform 5 (identifier: O76081-6)

The sequence of this isoform differs from the canonical sequence as follows:
     1-55: MPQLSQDNQE...GDLRAVPDIK → MRTADGGEPAGASSPAGRVDGGL
     56-170: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 388388Regulator of G-protein signaling 20
PRO_0000204233

Regions

Domain262 – 378117RGS
Compositional bias208 – 22013Poly-Cys

Natural variations

Alternative sequence1 – 236236Missing in isoform 3.
VSP_005698
Alternative sequence1 – 208208Missing in isoform 2.
VSP_005697
Alternative sequence1 – 5555MPQLS…VPDIK → MRTADGGEPAGASSPAGRVD GGL in isoform 5.
VSP_005694
Alternative sequence3 – 5553Missing in isoform 4.
VSP_005695
Alternative sequence56 – 170115Missing in isoform 1, isoform 4 and isoform 5.
VSP_005696
Alternative sequence209 – 22012CFCWC…CSCSC → MKETSGLFLISS in isoform 2.
VSP_005699
Alternative sequence237 – 24711ELRADLPTWEE → MKETSGLFLIS in isoform 3.
VSP_005700

Sequences

Sequence LengthMass (Da)Tools
Isoform 6 [UniParc].

Last modified October 18, 2001. Version 4.
Checksum: F44796D271F1765F

FASTA38843,692
        10         20         30         40         50         60 
MPQLSQDNQE CLQKHFSRPS IWTQFLPLFR AQRYNTDIHQ ITENEGDLRA VPDIKSFPPA 

        70         80         90        100        110        120 
QLPDSPAAPK LFGLLSSPLS SLARFFSHLL RRPPPEAPRR RLDFSPLLPA LPAARLSRGH 

       130        140        150        160        170        180 
EELPGRLSLL LGAALALPGR PSGGRPLRPP HPVAKPREED ATAGQSSPMP QMGSERMEMR 

       190        200        210        220        230        240 
KRQMPAAQDT PGAAPGQPGA GSRGSNACCF CWCCCCSCSC LTVRNQEDQR PTIASHELRA 

       250        260        270        280        290        300 
DLPTWEESPA PTLEEVNAWA QSFDKLMVTP AGRNAFREFL RTEFSEENML FWMACEELKK 

       310        320        330        340        350        360 
EANKNIIEEK ARIIYEDYIS ILSPKEVSLD SRVREVINRN MVEPSQHIFD DAQLQIYTLM 

       370        380 
HRDSYPRFMN SAVYKDLLQS LSEKSIEA 

« Hide

Isoform 1 [UniParc].

Checksum: 8BF212DBAF70A97E
Show »

FASTA27331,486
Isoform 2 [UniParc].

Checksum: A2F8633711953F26
Show »

FASTA18021,010
Isoform 3 [UniParc].

Checksum: E0554569F8C0CEA7
Show »

FASTA15217,735
Isoform 4 [UniParc].

Checksum: B5EA61FF181DA67C
Show »

FASTA22025,177
Isoform 5 [UniParc].

Checksum: 776410A4D698D622
Show »

FASTA24127,060

References

« Hide 'large scale' references
[1]"RGSZ1, a Gz-selective RGS protein in brain. Structure, membrane association, regulation by Galphaz phosphorylation, and relationship to a Gz GTPase-activating protein subfamily."
Wang J., Ducret A., Tu Y., Kozasa T., Aebersold R., Ross E.M.
J. Biol. Chem. 273:26014-26025(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 5), CHARACTERIZATION.
Tissue: Fetal brain.
[2]Wang J., Ducret A., Ross E.M.
Submitted (APR-2001) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION TO N-TERMINUS.
[3]"RGSZ1 and Ret RGS: two of several splice variants from the gene RGS20."
Barker S.A., Wang J., Sierra D.A., Ross E.M.
Genomics 78:223-229(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2; 3; 4 AND 6).
Tissue: Retina.
[4]"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
Puhl H.L. III, Ikeda S.R., Aronstam R.S.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 5).
Tissue: Fetal brain and Skin.
[6]"RGSZ1, a Gz-selective regulator of G protein signaling whose action is sensitive to the phosphorylation state of Gzalpha."
Glick J.L., Meigs T.E., Miron A., Casey P.J.
J. Biol. Chem. 273:26008-26013(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 57-388 (ISOFORM 1).
[7]"Regulator of G protein signaling Z1 (RGSZ1) interacts with Galpha i subunits and regulates Galpha i-mediated cell signaling."
Wang Y., Ho G., Zhang J.J., Nieuwenhuijsen B., Edris W., Chanda P.K., Young K.H.
J. Biol. Chem. 277:48325-48332(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF060877 mRNA. Translation: AAC62009.2.
AF366054 mRNA. Translation: AAK54122.1.
AF366055 mRNA. Translation: AAK54123.1.
AF366056 mRNA. Translation: AAK54124.1.
AF366057 mRNA. Translation: AAK54125.1.
AF074979 mRNA. Translation: AAC62013.1.
AF493940 mRNA. Translation: AAM12654.1.
BC015614 mRNA. Translation: AAH15614.2.
BC063490 mRNA. Translation: AAH63490.1.
AY046538 mRNA. Translation: AAL03971.1.
CCDSCCDS6155.1. [O76081-1]
CCDS6156.1. [O76081-6]
CCDS69482.1. [O76081-2]
RefSeqNP_001273602.1. NM_001286673.1. [O76081-2]
NP_001273603.1. NM_001286674.1. [O76081-4]
NP_001273604.1. NM_001286675.1. [O76081-3]
NP_003693.2. NM_003702.4. [O76081-6]
NP_733466.1. NM_170587.3. [O76081-1]
UniGeneHs.368733.

3D structure databases

ProteinModelPortalO76081.
SMRO76081. Positions 251-378.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid114161. 20 interactions.
IntActO76081. 8 interactions.
MINTMINT-3002244.
STRING9606.ENSP00000297313.

PTM databases

PhosphoSiteO76081.

Proteomic databases

MaxQBO76081.
PaxDbO76081.
PRIDEO76081.

Protocols and materials databases

DNASU8601.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000276500; ENSP00000276500; ENSG00000147509. [O76081-6]
ENST00000297313; ENSP00000297313; ENSG00000147509. [O76081-1]
ENST00000344277; ENSP00000344630; ENSG00000147509. [O76081-2]
GeneID8601.
KEGGhsa:8601.
UCSCuc003xrp.3. human. [O76081-1]
uc003xrq.3. human. [O76081-2]
uc010lye.3. human. [O76081-3]
uc010lyf.3. human. [O76081-4]

Organism-specific databases

CTD8601.
GeneCardsGC08P054764.
HGNCHGNC:14600. RGS20.
MIM607193. gene.
neXtProtNX_O76081.
PharmGKBPA34373.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG258376.
HOGENOMHOG000233513.
HOVERGENHBG013233.
InParanoidO76081.
KOK16449.
OMARFMNSAV.
OrthoDBEOG7SN8DQ.
PhylomeDBO76081.
TreeFamTF315837.

Gene expression databases

ArrayExpressO76081.
BgeeO76081.
CleanExHS_RGS20.
GenevestigatorO76081.

Family and domain databases

Gene3D1.10.196.10. 1 hit.
InterProIPR024066. Regulat_G_prot_signal_dom1.
IPR016137. Regulat_G_prot_signal_superfam.
IPR000342. RGS_dom.
[Graphical view]
PfamPF00615. RGS. 1 hit.
[Graphical view]
PRINTSPR01301. RGSPROTEIN.
SMARTSM00315. RGS. 1 hit.
[Graphical view]
SUPFAMSSF48097. SSF48097. 1 hit.
PROSITEPS50132. RGS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiRGS20.
GenomeRNAi8601.
NextBio32227.
PROO76081.
SOURCESearch...

Entry information

Entry nameRGS20_HUMAN
AccessionPrimary (citable) accession number: O76081
Secondary accession number(s): Q96BG9
Entry history
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: October 18, 2001
Last modified: July 9, 2014
This is version 131 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 8

Human chromosome 8: entries, gene names and cross-references to MIM