O76081 (RGS20_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Regulator of G-protein signaling 20
Short name=RGS20

Alternative name(s):
Gz-selective GTPase-activating protein
Short name=G(z)GAP
Short name=Gz-GAP
Regulator of G-protein signaling Z1
Regulator of Gz-selective protein signaling 1

Gene names

Name:	RGS20
Synonyms:	RGSZ1, ZGAP1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	388 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds selectively to G(z)-alpha and G(alpha)-i2 subunits, accelerates their GTPase activity and regulates their signaling activities. The G(z)-alpha activity is inhibited by the phosphorylation and palmitoylation of the G-protein. Negatively regulates mu-opioid receptor-mediated activation of the G-proteins By similarity. Ref.7
Subunit structure	Forms a complex with G(alpha)z/i2 subunits and mu-opioid receptors; the formation of this complex results in mu-opioid receptor desensitization. Interacts with OPRM1 By similarity.
Subcellular location	Membrane; Lipid-anchor. Nucleus. Cytoplasm. Note: Shuttles between the cytoplasm/cell membrane and the nucleus By similarity. Anchored to the membrane through palmitoylation By similarity.
Tissue specificity	Isoform 5 is expressed in brain at high levels in the caudate nucleus and temporal lobe.
Post-translational modification	Fatty acylated. Heavily palmitoylated in the cysteine string motif By similarity. N- and O-glycosylated in synapsomal membranes By similarity. Serine phosphorylated in synapsomal membranes By similarity. Sumoylated with SUMO1 and SUMO2 in synaptosomes. The sumoylated forms act as a scaffold for sequestering mu-opioid receptor-activated G(alpha) subunits By similarity.
Sequence similarities	Contains 1 RGS domain.

Ontologies

Keywords
Cellular component	Cytoplasm Membrane Nucleus
Coding sequence diversity	Alternative splicing
Molecular function	Signal transduction inhibitor
PTM	Glycoprotein Lipoprotein Palmitate Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of G-protein coupled receptor protein signaling pathway Traceable author statement Ref.6. Source: ProtInc termination of G-protein coupled receptor signaling pathway Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from Biological aspect of Ancestor. Source: RefGenome nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from Biological aspect of Ancestor. Source: RefGenome
Molecular_function	GTPase activator activity Inferred from Biological aspect of Ancestor. Source: RefGenome
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Hoxa1	P09022	3	EBI-1052678,EBI-3957603	From a different organism.

Alternative products

This entry describes 6 isoforms produced by alternative splicing. [Align] [Select]

Isoform 6 (identifier: O76081-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 1 (identifier: O76081-2)

The sequence of this isoform differs from the canonical sequence as follows:
     56-170: Missing.

Isoform 2 (identifier: O76081-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-208: Missing.
     209-220: CFCWCCCCSCSC → MKETSGLFLISS

Isoform 3 (identifier: O76081-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-236: Missing.
     237-247: ELRADLPTWEE → MKETSGLFLIS

Isoform 4 (identifier: O76081-5)

The sequence of this isoform differs from the canonical sequence as follows:
     3-55: Missing.
     56-170: Missing.

Isoform 5 (identifier: O76081-6)

The sequence of this isoform differs from the canonical sequence as follows:
     1-55: MPQLSQDNQE...GDLRAVPDIK → MRTADGGEPAGASSPAGRVDGGL
     56-170: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 388	388	Regulator of G-protein signaling 20		PRO_0000204233
Regions
	Domain	262 – 378	117	RGS
	Compositional bias	208 – 220	13	Poly-Cys
Natural variations
	Alternative sequence	1 – 236	236	Missing in isoform 3.		VSP_005698
	Alternative sequence	1 – 208	208	Missing in isoform 2.		VSP_005697
	Alternative sequence	1 – 55	55	MPQLS…VPDIK → MRTADGGEPAGASSPAGRVD GGL in isoform 5.		VSP_005694
	Alternative sequence	3 – 55	53	Missing in isoform 4.		VSP_005695
	Alternative sequence	56 – 170	115	Missing in isoform 1, isoform 4 and isoform 5.		VSP_005696
	Alternative sequence	209 – 220	12	CFCWC…CSCSC → MKETSGLFLISS in isoform 2.		VSP_005699
	Alternative sequence	237 – 247	11	ELRADLPTWEE → MKETSGLFLIS in isoform 3.		VSP_005700

Sequences

	Sequence		Length	Mass (Da)
	Isoform 6 [UniParc]. Last modified October 18, 2001. Version 4. Checksum: F44796D271F1765F	FASTA	388	43,692
	10 20 30 40 50 60 MPQLSQDNQE CLQKHFSRPS IWTQFLPLFR AQRYNTDIHQ ITENEGDLRA VPDIKSFPPA 70 80 90 100 110 120 QLPDSPAAPK LFGLLSSPLS SLARFFSHLL RRPPPEAPRR RLDFSPLLPA LPAARLSRGH 130 140 150 160 170 180 EELPGRLSLL LGAALALPGR PSGGRPLRPP HPVAKPREED ATAGQSSPMP QMGSERMEMR 190 200 210 220 230 240 KRQMPAAQDT PGAAPGQPGA GSRGSNACCF CWCCCCSCSC LTVRNQEDQR PTIASHELRA 250 260 270 280 290 300 DLPTWEESPA PTLEEVNAWA QSFDKLMVTP AGRNAFREFL RTEFSEENML FWMACEELKK 310 320 330 340 350 360 EANKNIIEEK ARIIYEDYIS ILSPKEVSLD SRVREVINRN MVEPSQHIFD DAQLQIYTLM 370 380 HRDSYPRFMN SAVYKDLLQS LSEKSIEA
	Isoform 1 [UniParc]. Checksum: 8BF212DBAF70A97E	FASTA	273	31,486
	10 20 30 40 50 60 MPQLSQDNQE CLQKHFSRPS IWTQFLPLFR AQRYNTDIHQ ITENEGDLRA VPDIKQMGSE 70 80 90 100 110 120 RMEMRKRQMP AAQDTPGAAP GQPGAGSRGS NACCFCWCCC CSCSCLTVRN QEDQRPTIAS 130 140 150 160 170 180 HELRADLPTW EESPAPTLEE VNAWAQSFDK LMVTPAGRNA FREFLRTEFS EENMLFWMAC 190 200 210 220 230 240 EELKKEANKN IIEEKARIIY EDYISILSPK EVSLDSRVRE VINRNMVEPS QHIFDDAQLQ 250 260 270 IYTLMHRDSY PRFMNSAVYK DLLQSLSEKS IEA
	Isoform 2 [UniParc]. Checksum: A2F8633711953F26	FASTA	180	21,010
	10 20 30 40 50 60 MKETSGLFLI SSLTVRNQED QRPTIASHEL RADLPTWEES PAPTLEEVNA WAQSFDKLMV 70 80 90 100 110 120 TPAGRNAFRE FLRTEFSEEN MLFWMACEEL KKEANKNIIE EKARIIYEDY ISILSPKEVS 130 140 150 160 170 180 LDSRVREVIN RNMVEPSQHI FDDAQLQIYT LMHRDSYPRF MNSAVYKDLL QSLSEKSIEA
	Isoform 3 [UniParc]. Checksum: E0554569F8C0CEA7	FASTA	152	17,735
	10 20 30 40 50 60 MKETSGLFLI SSPAPTLEEV NAWAQSFDKL MVTPAGRNAF REFLRTEFSE ENMLFWMACE 70 80 90 100 110 120 ELKKEANKNI IEEKARIIYE DYISILSPKE VSLDSRVREV INRNMVEPSQ HIFDDAQLQI 130 140 150 YTLMHRDSYP RFMNSAVYKD LLQSLSEKSI EA
	Isoform 4 [UniParc]. Checksum: B5EA61FF181DA67C	FASTA	220	25,177
	10 20 30 40 50 60 MPQMGSERME MRKRQMPAAQ DTPGAAPGQP GAGSRGSNAC CFCWCCCCSC SCLTVRNQED 70 80 90 100 110 120 QRPTIASHEL RADLPTWEES PAPTLEEVNA WAQSFDKLMV TPAGRNAFRE FLRTEFSEEN 130 140 150 160 170 180 MLFWMACEEL KKEANKNIIE EKARIIYEDY ISILSPKEVS LDSRVREVIN RNMVEPSQHI 190 200 210 220 FDDAQLQIYT LMHRDSYPRF MNSAVYKDLL QSLSEKSIEA
	Isoform 5 [UniParc]. Checksum: 776410A4D698D622	FASTA	241	27,060
	10 20 30 40 50 60 MRTADGGEPA GASSPAGRVD GGLQMGSERM EMRKRQMPAA QDTPGAAPGQ PGAGSRGSNA 70 80 90 100 110 120 CCFCWCCCCS CSCLTVRNQE DQRPTIASHE LRADLPTWEE SPAPTLEEVN AWAQSFDKLM 130 140 150 160 170 180 VTPAGRNAFR EFLRTEFSEE NMLFWMACEE LKKEANKNII EEKARIIYED YISILSPKEV 190 200 210 220 230 240 SLDSRVREVI NRNMVEPSQH IFDDAQLQIY TLMHRDSYPR FMNSAVYKDL LQSLSEKSIE A

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"RGSZ1, a Gz-selective RGS protein in brain. Structure, membrane association, regulation by Galphaz phosphorylation, and relationship to a Gz GTPase-activating protein subfamily." Wang J., Ducret A., Tu Y., Kozasa T., Aebersold R., Ross E.M. J. Biol. Chem. 273:26014-26025(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 5), CHARACTERIZATION. Tissue: Fetal brain.
[2]	Wang J., Ducret A., Ross E.M. Submitted (APR-2001) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO N-TERMINUS.
[3]	"RGSZ1 and Ret RGS: two of several splice variants from the gene RGS20." Barker S.A., Wang J., Sierra D.A., Ross E.M. Genomics 78:223-229(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2; 3; 4 AND 6). Tissue: Retina.
[4]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Puhl H.L. III, Ikeda S.R., Aronstam R.S. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 5). Tissue: Fetal brain and Skin.
[6]	"RGSZ1, a Gz-selective regulator of G protein signaling whose action is sensitive to the phosphorylation state of Gzalpha." Glick J.L., Meigs T.E., Miron A., Casey P.J. J. Biol. Chem. 273:26008-26013(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 57-388 (ISOFORM 1).
[7]	"Regulator of G protein signaling Z1 (RGSZ1) interacts with Galpha i subunits and regulates Galpha i-mediated cell signaling." Wang Y., Ho G., Zhang J.J., Nieuwenhuijsen B., Edris W., Chanda P.K., Young K.H. J. Biol. Chem. 277:48325-48332(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF060877 mRNA. Translation: AAC62009.2. AF366054 mRNA. Translation: AAK54122.1. AF366055 mRNA. Translation: AAK54123.1. AF366056 mRNA. Translation: AAK54124.1. AF366057 mRNA. Translation: AAK54125.1. AF074979 mRNA. Translation: AAC62013.1. AF493940 mRNA. Translation: AAM12654.1. BC015614 mRNA. Translation: AAH15614.2. BC063490 mRNA. Translation: AAH63490.1. AY046538 mRNA. Translation: AAL03971.1.
IPI	IPI00008802. IPI00220880. IPI00220881. IPI00220882. IPI00220883. IPI00220884.
RefSeq	NP_003693.2. NM_003702.3. NP_733466.1. NM_170587.2.
UniGene	Hs.741492.
3D structure databases
ProteinModelPortal	O76081.
ModBase	Search...
Protein-protein interaction databases
IntAct	O76081. 3 interactions.
MINT	MINT-3002244.
STRING	9606.ENSP00000297313.
PTM databases
PhosphoSite	O76081.
Proteomic databases
PaxDb	O76081.
PRIDE	O76081.
Protocols and materials databases
DNASU	8601.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000276500; ENSP00000276500; ENSG00000147509. ENST00000297313; ENSP00000297313; ENSG00000147509. ENST00000344277; ENSP00000344630; ENSG00000147509.
GeneID	8601.
KEGG	hsa:8601.
UCSC	uc003xrp.3. human. uc003xrq.3. human. uc003xrs.3. human. uc010lye.3. human. uc010lyf.3. human.
Organism-specific databases
CTD	8601.
GeneCards	GC08P054764.
HGNC	HGNC:14600. RGS20.
MIM	607193. gene.
neXtProt	NX_O76081.
PharmGKB	PA34373.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG258376.
HOGENOM	HOG000233513.
HOVERGEN	HBG013233.
InParanoid	O76081.
KO	K16449.
OMA	MPPMGSE.
OrthoDB	EOG4VQ9QF.
PhylomeDB	O76081.
Gene expression databases
ArrayExpress	O76081.
Bgee	O76081.
CleanEx	HS_RGS20.
Genevestigator	O76081.
Family and domain databases
Gene3D	1.10.196.10. 1 hit.
InterPro	IPR000342. Regulat_G_prot_signal. IPR024066. Regulat_G_prot_signal_dom1. IPR016137. Regulat_G_prot_signal_superfam. [Graphical view]
Pfam	PF00615. RGS. 1 hit. [Graphical view]
PRINTS	PR01301. RGSPROTEIN.
SMART	SM00315. RGS. 1 hit. [Graphical view]
SUPFAM	SSF48097. Regulat_G_prot_signal_superfam. 1 hit.
PROSITE	PS50132. RGS. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	8601.
NextBio	32227.
SOURCE	Search...

Entry information

Entry name

RGS20_HUMAN

Accession

Primary (citable) accession number: O76081
Secondary accession number(s): Q96BG9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2001
Last sequence update:	October 18, 2001
Last modified:	May 1, 2013
This is version 121 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.