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O76081

- RGS20_HUMAN

UniProt

O76081 - RGS20_HUMAN

Protein

Regulator of G-protein signaling 20

Gene

RGS20

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 133 (01 Oct 2014)
      Sequence version 4 (18 Oct 2001)
      Previous versions | rss
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    Functioni

    Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds selectively to G(z)-alpha and G(alpha)-i2 subunits, accelerates their GTPase activity and regulates their signaling activities. The G(z)-alpha activity is inhibited by the phosphorylation and palmitoylation of the G-protein. Negatively regulates mu-opioid receptor-mediated activation of the G-proteins By similarity.By similarity

    GO - Molecular functioni

    1. GTPase activator activity Source: RefGenome
    2. protein binding Source: IntAct

    GO - Biological processi

    1. positive regulation of GTPase activity Source: GOC
    2. regulation of G-protein coupled receptor protein signaling pathway Source: ProtInc
    3. termination of G-protein coupled receptor signaling pathway Source: InterPro

    Keywords - Molecular functioni

    Signal transduction inhibitor

    Enzyme and pathway databases

    ReactomeiREACT_19231. G alpha (i) signalling events.
    REACT_19333. G alpha (z) signalling events.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Regulator of G-protein signaling 20
    Short name:
    RGS20
    Alternative name(s):
    Gz-selective GTPase-activating protein
    Short name:
    G(z)GAP
    Short name:
    Gz-GAP
    Regulator of G-protein signaling Z1
    Regulator of Gz-selective protein signaling 1
    Gene namesi
    Name:RGS20
    Synonyms:RGSZ1, ZGAP1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 8

    Organism-specific databases

    HGNCiHGNC:14600. RGS20.

    Subcellular locationi

    Membrane; Lipid-anchor. Nucleus. Cytoplasm
    Note: Shuttles between the cytoplasm/cell membrane and the nucleus. Anchored to the membrane through palmitoylation.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: RefGenome
    2. nucleus Source: UniProtKB-SubCell
    3. plasma membrane Source: RefGenome

    Keywords - Cellular componenti

    Cytoplasm, Membrane, Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA34373.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 388388Regulator of G-protein signaling 20PRO_0000204233Add
    BLAST

    Post-translational modificationi

    Fatty acylated. Heavily palmitoylated in the cysteine string motif By similarity.By similarity
    N- and O-glycosylated in synapsomal membranes.By similarity
    Serine phosphorylated in synapsomal membranes.By similarity
    Sumoylated with SUMO1 and SUMO2 in synaptosomes. The sumoylated forms act as a scaffold for sequestering mu-opioid receptor-activated G(alpha) subunits By similarity.By similarity

    Keywords - PTMi

    Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein, Ubl conjugation

    Proteomic databases

    MaxQBiO76081.
    PaxDbiO76081.
    PRIDEiO76081.

    PTM databases

    PhosphoSiteiO76081.

    Expressioni

    Tissue specificityi

    Isoform 5 is expressed in brain at high levels in the caudate nucleus and temporal lobe.

    Gene expression databases

    ArrayExpressiO76081.
    BgeeiO76081.
    CleanExiHS_RGS20.
    GenevestigatoriO76081.

    Interactioni

    Subunit structurei

    Forms a complex with G(alpha)z/i2 subunits and mu-opioid receptors; the formation of this complex results in mu-opioid receptor desensitization. Interacts with OPRM1 By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Hoxa1P090223EBI-1052678,EBI-3957603From a different organism.
    MTNR1AP480396EBI-1052678,EBI-1188238
    MTNR1BP492862EBI-1052678,EBI-1188341

    Protein-protein interaction databases

    BioGridi114161. 21 interactions.
    IntActiO76081. 8 interactions.
    MINTiMINT-3002244.
    STRINGi9606.ENSP00000297313.

    Structurei

    3D structure databases

    ProteinModelPortaliO76081.
    SMRiO76081. Positions 251-378.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini262 – 378117RGSPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi208 – 22013Poly-CysAdd
    BLAST

    Sequence similaritiesi

    Contains 1 RGS domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG258376.
    HOGENOMiHOG000233513.
    HOVERGENiHBG013233.
    InParanoidiO76081.
    KOiK16449.
    OMAiRFMNSAV.
    OrthoDBiEOG7SN8DQ.
    PhylomeDBiO76081.
    TreeFamiTF315837.

    Family and domain databases

    Gene3Di1.10.196.10. 1 hit.
    InterProiIPR024066. Regulat_G_prot_signal_dom1.
    IPR016137. Regulat_G_prot_signal_superfam.
    IPR000342. RGS_dom.
    [Graphical view]
    PfamiPF00615. RGS. 1 hit.
    [Graphical view]
    PRINTSiPR01301. RGSPROTEIN.
    SMARTiSM00315. RGS. 1 hit.
    [Graphical view]
    SUPFAMiSSF48097. SSF48097. 1 hit.
    PROSITEiPS50132. RGS. 1 hit.
    [Graphical view]

    Sequences (6)i

    Sequence statusi: Complete.

    This entry describes 6 isoformsi produced by alternative splicing. Align

    Isoform 6 (identifier: O76081-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MPQLSQDNQE CLQKHFSRPS IWTQFLPLFR AQRYNTDIHQ ITENEGDLRA    50
    VPDIKSFPPA QLPDSPAAPK LFGLLSSPLS SLARFFSHLL RRPPPEAPRR 100
    RLDFSPLLPA LPAARLSRGH EELPGRLSLL LGAALALPGR PSGGRPLRPP 150
    HPVAKPREED ATAGQSSPMP QMGSERMEMR KRQMPAAQDT PGAAPGQPGA 200
    GSRGSNACCF CWCCCCSCSC LTVRNQEDQR PTIASHELRA DLPTWEESPA 250
    PTLEEVNAWA QSFDKLMVTP AGRNAFREFL RTEFSEENML FWMACEELKK 300
    EANKNIIEEK ARIIYEDYIS ILSPKEVSLD SRVREVINRN MVEPSQHIFD 350
    DAQLQIYTLM HRDSYPRFMN SAVYKDLLQS LSEKSIEA 388
    Length:388
    Mass (Da):43,692
    Last modified:October 18, 2001 - v4
    Checksum:iF44796D271F1765F
    GO
    Isoform 1 (identifier: O76081-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         56-170: Missing.

    Show »
    Length:273
    Mass (Da):31,486
    Checksum:i8BF212DBAF70A97E
    GO
    Isoform 2 (identifier: O76081-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-208: Missing.
         209-220: CFCWCCCCSCSC → MKETSGLFLISS

    Show »
    Length:180
    Mass (Da):21,010
    Checksum:iA2F8633711953F26
    GO
    Isoform 3 (identifier: O76081-4) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-236: Missing.
         237-247: ELRADLPTWEE → MKETSGLFLIS

    Show »
    Length:152
    Mass (Da):17,735
    Checksum:iE0554569F8C0CEA7
    GO
    Isoform 4 (identifier: O76081-5) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         3-55: Missing.
         56-170: Missing.

    Show »
    Length:220
    Mass (Da):25,177
    Checksum:iB5EA61FF181DA67C
    GO
    Isoform 5 (identifier: O76081-6) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-55: MPQLSQDNQE...GDLRAVPDIK → MRTADGGEPAGASSPAGRVDGGL
         56-170: Missing.

    Show »
    Length:241
    Mass (Da):27,060
    Checksum:i776410A4D698D622
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 236236Missing in isoform 3. 1 PublicationVSP_005698Add
    BLAST
    Alternative sequencei1 – 208208Missing in isoform 2. 1 PublicationVSP_005697Add
    BLAST
    Alternative sequencei1 – 5555MPQLS…VPDIK → MRTADGGEPAGASSPAGRVD GGL in isoform 5. 2 PublicationsVSP_005694Add
    BLAST
    Alternative sequencei3 – 5553Missing in isoform 4. 2 PublicationsVSP_005695Add
    BLAST
    Alternative sequencei56 – 170115Missing in isoform 1, isoform 4 and isoform 5. 5 PublicationsVSP_005696Add
    BLAST
    Alternative sequencei209 – 22012CFCWC…CSCSC → MKETSGLFLISS in isoform 2. 1 PublicationVSP_005699Add
    BLAST
    Alternative sequencei237 – 24711ELRADLPTWEE → MKETSGLFLIS in isoform 3. 1 PublicationVSP_005700Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF060877 mRNA. Translation: AAC62009.2.
    AF366054 mRNA. Translation: AAK54122.1.
    AF366055 mRNA. Translation: AAK54123.1.
    AF366056 mRNA. Translation: AAK54124.1.
    AF366057 mRNA. Translation: AAK54125.1.
    AF074979 mRNA. Translation: AAC62013.1.
    AF493940 mRNA. Translation: AAM12654.1.
    BC015614 mRNA. Translation: AAH15614.2.
    BC063490 mRNA. Translation: AAH63490.1.
    AY046538 mRNA. Translation: AAL03971.1.
    CCDSiCCDS6155.1. [O76081-1]
    CCDS6156.1. [O76081-6]
    CCDS69482.1. [O76081-2]
    RefSeqiNP_001273602.1. NM_001286673.1. [O76081-2]
    NP_001273603.1. NM_001286674.1. [O76081-4]
    NP_001273604.1. NM_001286675.1. [O76081-3]
    NP_003693.2. NM_003702.4. [O76081-6]
    NP_733466.1. NM_170587.3. [O76081-1]
    UniGeneiHs.368733.

    Genome annotation databases

    EnsembliENST00000276500; ENSP00000276500; ENSG00000147509. [O76081-6]
    ENST00000297313; ENSP00000297313; ENSG00000147509. [O76081-1]
    ENST00000344277; ENSP00000344630; ENSG00000147509. [O76081-2]
    GeneIDi8601.
    KEGGihsa:8601.
    UCSCiuc003xrp.3. human. [O76081-1]
    uc003xrq.3. human. [O76081-2]
    uc010lye.3. human. [O76081-3]
    uc010lyf.3. human. [O76081-4]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF060877 mRNA. Translation: AAC62009.2 .
    AF366054 mRNA. Translation: AAK54122.1 .
    AF366055 mRNA. Translation: AAK54123.1 .
    AF366056 mRNA. Translation: AAK54124.1 .
    AF366057 mRNA. Translation: AAK54125.1 .
    AF074979 mRNA. Translation: AAC62013.1 .
    AF493940 mRNA. Translation: AAM12654.1 .
    BC015614 mRNA. Translation: AAH15614.2 .
    BC063490 mRNA. Translation: AAH63490.1 .
    AY046538 mRNA. Translation: AAL03971.1 .
    CCDSi CCDS6155.1. [O76081-1 ]
    CCDS6156.1. [O76081-6 ]
    CCDS69482.1. [O76081-2 ]
    RefSeqi NP_001273602.1. NM_001286673.1. [O76081-2 ]
    NP_001273603.1. NM_001286674.1. [O76081-4 ]
    NP_001273604.1. NM_001286675.1. [O76081-3 ]
    NP_003693.2. NM_003702.4. [O76081-6 ]
    NP_733466.1. NM_170587.3. [O76081-1 ]
    UniGenei Hs.368733.

    3D structure databases

    ProteinModelPortali O76081.
    SMRi O76081. Positions 251-378.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 114161. 21 interactions.
    IntActi O76081. 8 interactions.
    MINTi MINT-3002244.
    STRINGi 9606.ENSP00000297313.

    PTM databases

    PhosphoSitei O76081.

    Proteomic databases

    MaxQBi O76081.
    PaxDbi O76081.
    PRIDEi O76081.

    Protocols and materials databases

    DNASUi 8601.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000276500 ; ENSP00000276500 ; ENSG00000147509 . [O76081-6 ]
    ENST00000297313 ; ENSP00000297313 ; ENSG00000147509 . [O76081-1 ]
    ENST00000344277 ; ENSP00000344630 ; ENSG00000147509 . [O76081-2 ]
    GeneIDi 8601.
    KEGGi hsa:8601.
    UCSCi uc003xrp.3. human. [O76081-1 ]
    uc003xrq.3. human. [O76081-2 ]
    uc010lye.3. human. [O76081-3 ]
    uc010lyf.3. human. [O76081-4 ]

    Organism-specific databases

    CTDi 8601.
    GeneCardsi GC08P054764.
    HGNCi HGNC:14600. RGS20.
    MIMi 607193. gene.
    neXtProti NX_O76081.
    PharmGKBi PA34373.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG258376.
    HOGENOMi HOG000233513.
    HOVERGENi HBG013233.
    InParanoidi O76081.
    KOi K16449.
    OMAi RFMNSAV.
    OrthoDBi EOG7SN8DQ.
    PhylomeDBi O76081.
    TreeFami TF315837.

    Enzyme and pathway databases

    Reactomei REACT_19231. G alpha (i) signalling events.
    REACT_19333. G alpha (z) signalling events.

    Miscellaneous databases

    GeneWikii RGS20.
    GenomeRNAii 8601.
    NextBioi 32227.
    PROi O76081.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O76081.
    Bgeei O76081.
    CleanExi HS_RGS20.
    Genevestigatori O76081.

    Family and domain databases

    Gene3Di 1.10.196.10. 1 hit.
    InterProi IPR024066. Regulat_G_prot_signal_dom1.
    IPR016137. Regulat_G_prot_signal_superfam.
    IPR000342. RGS_dom.
    [Graphical view ]
    Pfami PF00615. RGS. 1 hit.
    [Graphical view ]
    PRINTSi PR01301. RGSPROTEIN.
    SMARTi SM00315. RGS. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48097. SSF48097. 1 hit.
    PROSITEi PS50132. RGS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "RGSZ1, a Gz-selective RGS protein in brain. Structure, membrane association, regulation by Galphaz phosphorylation, and relationship to a Gz GTPase-activating protein subfamily."
      Wang J., Ducret A., Tu Y., Kozasa T., Aebersold R., Ross E.M.
      J. Biol. Chem. 273:26014-26025(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 5), CHARACTERIZATION.
      Tissue: Fetal brain.
    2. Wang J., Ducret A., Ross E.M.
      Submitted (APR-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION TO N-TERMINUS.
    3. "RGSZ1 and Ret RGS: two of several splice variants from the gene RGS20."
      Barker S.A., Wang J., Sierra D.A., Ross E.M.
      Genomics 78:223-229(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2; 3; 4 AND 6).
      Tissue: Retina.
    4. "cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
      Puhl H.L. III, Ikeda S.R., Aronstam R.S.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 5).
      Tissue: Fetal brain and Skin.
    6. "RGSZ1, a Gz-selective regulator of G protein signaling whose action is sensitive to the phosphorylation state of Gzalpha."
      Glick J.L., Meigs T.E., Miron A., Casey P.J.
      J. Biol. Chem. 273:26008-26013(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 57-388 (ISOFORM 1).
    7. "Regulator of G protein signaling Z1 (RGSZ1) interacts with Galpha i subunits and regulates Galpha i-mediated cell signaling."
      Wang Y., Ho G., Zhang J.J., Nieuwenhuijsen B., Edris W., Chanda P.K., Young K.H.
      J. Biol. Chem. 277:48325-48332(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiRGS20_HUMAN
    AccessioniPrimary (citable) accession number: O76081
    Secondary accession number(s): Q96BG9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 20, 2001
    Last sequence update: October 18, 2001
    Last modified: October 1, 2014
    This is version 133 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 8
      Human chromosome 8: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3