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Protein

Regulator of G-protein signaling 20

Gene

RGS20

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds selectively to G(z)-alpha and G(alpha)-i2 subunits, accelerates their GTPase activity and regulates their signaling activities. The G(z)-alpha activity is inhibited by the phosphorylation and palmitoylation of the G-protein. Negatively regulates mu-opioid receptor-mediated activation of the G-proteins (By similarity).By similarity

GO - Molecular functioni

  • GTPase activator activity Source: ProtInc

GO - Biological processi

  • positive regulation of GTPase activity Source: GOC
  • regulation of G-protein coupled receptor protein signaling pathway Source: ProtInc
  • termination of G-protein coupled receptor signaling pathway Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Signal transduction inhibitor

Enzyme and pathway databases

ReactomeiREACT_19231. G alpha (i) signalling events.
REACT_19333. G alpha (z) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Regulator of G-protein signaling 20
Short name:
RGS20
Alternative name(s):
Gz-selective GTPase-activating protein
Short name:
G(z)GAP
Short name:
Gz-GAP
Regulator of G-protein signaling Z1
Regulator of Gz-selective protein signaling 1
Gene namesi
Name:RGS20
Synonyms:RGSZ1, ZGAP1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 8

Organism-specific databases

HGNCiHGNC:14600. RGS20.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: CACAO
  • nucleus Source: UniProtKB-SubCell
  • plasma membrane Source: GO_Central
  • trans-Golgi network Source: CACAO
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA34373.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 388388Regulator of G-protein signaling 20PRO_0000204233Add
BLAST

Post-translational modificationi

Fatty acylated. Heavily palmitoylated in the cysteine string motif (By similarity).By similarity
N- and O-glycosylated in synapsomal membranes.By similarity
Serine phosphorylated in synapsomal membranes.By similarity
Sumoylated with SUMO1 and SUMO2 in synaptosomes. The sumoylated forms act as a scaffold for sequestering mu-opioid receptor-activated G(alpha) subunits (By similarity).By similarity

Keywords - PTMi

Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiO76081.
PaxDbiO76081.
PRIDEiO76081.

PTM databases

PhosphoSiteiO76081.

Expressioni

Tissue specificityi

Isoform 5 is expressed in brain at high levels in the caudate nucleus and temporal lobe.

Gene expression databases

BgeeiO76081.
CleanExiHS_RGS20.
ExpressionAtlasiO76081. baseline and differential.
GenevisibleiO76081. HS.

Interactioni

Subunit structurei

Forms a complex with G(alpha)z/i2 subunits and mu-opioid receptors; the formation of this complex results in mu-opioid receptor desensitization. Interacts with OPRM1 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Q9NWL93EBI-1052678,EBI-10315054
AQP1P299723EBI-10178530,EBI-745213
CATSPER1Q8NEC54EBI-1052678,EBI-744545
CRCT1Q9UGL93EBI-10178530,EBI-713677
CREB5Q02930-33EBI-10178530,EBI-10192698
FASLGP480233EBI-10178530,EBI-495538
GLRX3O760033EBI-10178530,EBI-374781
GNAI2P048993EBI-10178530,EBI-353997
HOXA1P496393EBI-10178530,EBI-740785
Hoxa1P090223EBI-1052678,EBI-3957603From a different organism.
HSPD1P108093EBI-1052678,EBI-352528
INSI3WAC93EBI-10178530,EBI-10178524
KLHL38Q2WGJ63EBI-10178530,EBI-6426443
KRTAP4-2Q9BYR53EBI-1052678,EBI-10172511
KRTAP5-6Q6L8G93EBI-10178530,EBI-10250562
KRTAP5-9P263713EBI-10178530,EBI-3958099
LCE1BQ5T7P33EBI-1052678,EBI-10245913
LCE2AQ5TA793EBI-10178530,EBI-10246607
LCE2DQ5TA823EBI-1052678,EBI-10246750
LCE3EQ5T5B03EBI-10178530,EBI-10245456
LCE4AQ5TA783EBI-10178530,EBI-10246358
MAPKBP1O603363EBI-1052678,EBI-947402
MTNR1AP480396EBI-1052678,EBI-1188238
MTNR1BP492862EBI-1052678,EBI-1188341
OTX1P322424EBI-1052678,EBI-740446
PTGER3Q147X83EBI-10178530,EBI-10234038
PTPMT1Q8WUK03EBI-10178530,EBI-7199479
PVRL3Q9NQS33EBI-1052678,EBI-2826725

Protein-protein interaction databases

BioGridi114161. 44 interactions.
IntActiO76081. 31 interactions.
MINTiMINT-3002244.
STRINGi9606.ENSP00000297313.

Structurei

3D structure databases

ProteinModelPortaliO76081.
SMRiO76081. Positions 251-378.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini262 – 378117RGSPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi208 – 22013Poly-CysAdd
BLAST

Sequence similaritiesi

Contains 1 RGS domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG258376.
GeneTreeiENSGT00760000118903.
HOGENOMiHOG000233513.
HOVERGENiHBG013233.
InParanoidiO76081.
KOiK16449.
OMAiRQMPAAQ.
OrthoDBiEOG7SN8DQ.
PhylomeDBiO76081.
TreeFamiTF315837.

Family and domain databases

Gene3Di1.10.196.10. 1 hit.
InterProiIPR016137. RGS.
IPR024066. RGS_subdom1.
[Graphical view]
PfamiPF00615. RGS. 1 hit.
[Graphical view]
PRINTSiPR01301. RGSPROTEIN.
SMARTiSM00315. RGS. 1 hit.
[Graphical view]
SUPFAMiSSF48097. SSF48097. 1 hit.
PROSITEiPS50132. RGS. 1 hit.
[Graphical view]

Sequences (6)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 6 (identifier: O76081-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPQLSQDNQE CLQKHFSRPS IWTQFLPLFR AQRYNTDIHQ ITENEGDLRA
60 70 80 90 100
VPDIKSFPPA QLPDSPAAPK LFGLLSSPLS SLARFFSHLL RRPPPEAPRR
110 120 130 140 150
RLDFSPLLPA LPAARLSRGH EELPGRLSLL LGAALALPGR PSGGRPLRPP
160 170 180 190 200
HPVAKPREED ATAGQSSPMP QMGSERMEMR KRQMPAAQDT PGAAPGQPGA
210 220 230 240 250
GSRGSNACCF CWCCCCSCSC LTVRNQEDQR PTIASHELRA DLPTWEESPA
260 270 280 290 300
PTLEEVNAWA QSFDKLMVTP AGRNAFREFL RTEFSEENML FWMACEELKK
310 320 330 340 350
EANKNIIEEK ARIIYEDYIS ILSPKEVSLD SRVREVINRN MVEPSQHIFD
360 370 380
DAQLQIYTLM HRDSYPRFMN SAVYKDLLQS LSEKSIEA
Length:388
Mass (Da):43,692
Last modified:October 18, 2001 - v4
Checksum:iF44796D271F1765F
GO
Isoform 1 (identifier: O76081-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     56-170: Missing.

Show »
Length:273
Mass (Da):31,486
Checksum:i8BF212DBAF70A97E
GO
Isoform 2 (identifier: O76081-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-208: Missing.
     209-220: CFCWCCCCSCSC → MKETSGLFLISS

Show »
Length:180
Mass (Da):21,010
Checksum:iA2F8633711953F26
GO
Isoform 3 (identifier: O76081-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-236: Missing.
     237-247: ELRADLPTWEE → MKETSGLFLIS

Show »
Length:152
Mass (Da):17,735
Checksum:iE0554569F8C0CEA7
GO
Isoform 4 (identifier: O76081-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     3-55: Missing.
     56-170: Missing.

Show »
Length:220
Mass (Da):25,177
Checksum:iB5EA61FF181DA67C
GO
Isoform 5 (identifier: O76081-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-55: MPQLSQDNQE...GDLRAVPDIK → MRTADGGEPAGASSPAGRVDGGL
     56-170: Missing.

Show »
Length:241
Mass (Da):27,060
Checksum:i776410A4D698D622
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 236236Missing in isoform 3. 1 PublicationVSP_005698Add
BLAST
Alternative sequencei1 – 208208Missing in isoform 2. 1 PublicationVSP_005697Add
BLAST
Alternative sequencei1 – 5555MPQLS…VPDIK → MRTADGGEPAGASSPAGRVD GGL in isoform 5. 2 PublicationsVSP_005694Add
BLAST
Alternative sequencei3 – 5553Missing in isoform 4. 2 PublicationsVSP_005695Add
BLAST
Alternative sequencei56 – 170115Missing in isoform 1, isoform 4 and isoform 5. 5 PublicationsVSP_005696Add
BLAST
Alternative sequencei209 – 22012CFCWC…CSCSC → MKETSGLFLISS in isoform 2. 1 PublicationVSP_005699Add
BLAST
Alternative sequencei237 – 24711ELRADLPTWEE → MKETSGLFLIS in isoform 3. 1 PublicationVSP_005700Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF060877 mRNA. Translation: AAC62009.2.
AF366054 mRNA. Translation: AAK54122.1.
AF366055 mRNA. Translation: AAK54123.1.
AF366056 mRNA. Translation: AAK54124.1.
AF366057 mRNA. Translation: AAK54125.1.
AF074979 mRNA. Translation: AAC62013.1.
AF493940 mRNA. Translation: AAM12654.1.
BC015614 mRNA. Translation: AAH15614.2.
BC063490 mRNA. Translation: AAH63490.1.
AY046538 mRNA. Translation: AAL03971.1.
CCDSiCCDS6155.1. [O76081-1]
CCDS6156.1. [O76081-6]
CCDS69482.1. [O76081-2]
RefSeqiNP_001273602.1. NM_001286673.1. [O76081-2]
NP_001273603.1. NM_001286674.1. [O76081-4]
NP_001273604.1. NM_001286675.1. [O76081-3]
NP_003693.2. NM_003702.4. [O76081-6]
NP_733466.1. NM_170587.3. [O76081-1]
UniGeneiHs.368733.

Genome annotation databases

EnsembliENST00000276500; ENSP00000276500; ENSG00000147509. [O76081-6]
ENST00000297313; ENSP00000297313; ENSG00000147509. [O76081-1]
ENST00000344277; ENSP00000344630; ENSG00000147509. [O76081-2]
GeneIDi8601.
KEGGihsa:8601.
UCSCiuc003xrp.3. human. [O76081-1]
uc003xrq.3. human. [O76081-2]
uc010lye.3. human. [O76081-3]
uc010lyf.3. human. [O76081-4]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF060877 mRNA. Translation: AAC62009.2.
AF366054 mRNA. Translation: AAK54122.1.
AF366055 mRNA. Translation: AAK54123.1.
AF366056 mRNA. Translation: AAK54124.1.
AF366057 mRNA. Translation: AAK54125.1.
AF074979 mRNA. Translation: AAC62013.1.
AF493940 mRNA. Translation: AAM12654.1.
BC015614 mRNA. Translation: AAH15614.2.
BC063490 mRNA. Translation: AAH63490.1.
AY046538 mRNA. Translation: AAL03971.1.
CCDSiCCDS6155.1. [O76081-1]
CCDS6156.1. [O76081-6]
CCDS69482.1. [O76081-2]
RefSeqiNP_001273602.1. NM_001286673.1. [O76081-2]
NP_001273603.1. NM_001286674.1. [O76081-4]
NP_001273604.1. NM_001286675.1. [O76081-3]
NP_003693.2. NM_003702.4. [O76081-6]
NP_733466.1. NM_170587.3. [O76081-1]
UniGeneiHs.368733.

3D structure databases

ProteinModelPortaliO76081.
SMRiO76081. Positions 251-378.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114161. 44 interactions.
IntActiO76081. 31 interactions.
MINTiMINT-3002244.
STRINGi9606.ENSP00000297313.

PTM databases

PhosphoSiteiO76081.

Proteomic databases

MaxQBiO76081.
PaxDbiO76081.
PRIDEiO76081.

Protocols and materials databases

DNASUi8601.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000276500; ENSP00000276500; ENSG00000147509. [O76081-6]
ENST00000297313; ENSP00000297313; ENSG00000147509. [O76081-1]
ENST00000344277; ENSP00000344630; ENSG00000147509. [O76081-2]
GeneIDi8601.
KEGGihsa:8601.
UCSCiuc003xrp.3. human. [O76081-1]
uc003xrq.3. human. [O76081-2]
uc010lye.3. human. [O76081-3]
uc010lyf.3. human. [O76081-4]

Organism-specific databases

CTDi8601.
GeneCardsiGC08P054764.
HGNCiHGNC:14600. RGS20.
MIMi607193. gene.
neXtProtiNX_O76081.
PharmGKBiPA34373.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG258376.
GeneTreeiENSGT00760000118903.
HOGENOMiHOG000233513.
HOVERGENiHBG013233.
InParanoidiO76081.
KOiK16449.
OMAiRQMPAAQ.
OrthoDBiEOG7SN8DQ.
PhylomeDBiO76081.
TreeFamiTF315837.

Enzyme and pathway databases

ReactomeiREACT_19231. G alpha (i) signalling events.
REACT_19333. G alpha (z) signalling events.

Miscellaneous databases

ChiTaRSiRGS20. human.
GeneWikiiRGS20.
GenomeRNAii8601.
NextBioi32227.
PROiO76081.
SOURCEiSearch...

Gene expression databases

BgeeiO76081.
CleanExiHS_RGS20.
ExpressionAtlasiO76081. baseline and differential.
GenevisibleiO76081. HS.

Family and domain databases

Gene3Di1.10.196.10. 1 hit.
InterProiIPR016137. RGS.
IPR024066. RGS_subdom1.
[Graphical view]
PfamiPF00615. RGS. 1 hit.
[Graphical view]
PRINTSiPR01301. RGSPROTEIN.
SMARTiSM00315. RGS. 1 hit.
[Graphical view]
SUPFAMiSSF48097. SSF48097. 1 hit.
PROSITEiPS50132. RGS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "RGSZ1, a Gz-selective RGS protein in brain. Structure, membrane association, regulation by Galphaz phosphorylation, and relationship to a Gz GTPase-activating protein subfamily."
    Wang J., Ducret A., Tu Y., Kozasa T., Aebersold R., Ross E.M.
    J. Biol. Chem. 273:26014-26025(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 5), CHARACTERIZATION.
    Tissue: Fetal brain.
  2. Wang J., Ducret A., Ross E.M.
    Submitted (APR-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO N-TERMINUS.
  3. "RGSZ1 and Ret RGS: two of several splice variants from the gene RGS20."
    Barker S.A., Wang J., Sierra D.A., Ross E.M.
    Genomics 78:223-229(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2; 3; 4 AND 6).
    Tissue: Retina.
  4. "cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
    Puhl H.L. III, Ikeda S.R., Aronstam R.S.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 5).
    Tissue: Fetal brain and Skin.
  6. "RGSZ1, a Gz-selective regulator of G protein signaling whose action is sensitive to the phosphorylation state of Gzalpha."
    Glick J.L., Meigs T.E., Miron A., Casey P.J.
    J. Biol. Chem. 273:26008-26013(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 57-388 (ISOFORM 1).
  7. "Regulator of G protein signaling Z1 (RGSZ1) interacts with Galpha i subunits and regulates Galpha i-mediated cell signaling."
    Wang Y., Ho G., Zhang J.J., Nieuwenhuijsen B., Edris W., Chanda P.K., Young K.H.
    J. Biol. Chem. 277:48325-48332(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiRGS20_HUMAN
AccessioniPrimary (citable) accession number: O76081
Secondary accession number(s): Q96BG9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: October 18, 2001
Last modified: June 24, 2015
This is version 140 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.