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O76081

- RGS20_HUMAN

UniProt

O76081 - RGS20_HUMAN

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Protein

Regulator of G-protein signaling 20

Gene

RGS20

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds selectively to G(z)-alpha and G(alpha)-i2 subunits, accelerates their GTPase activity and regulates their signaling activities. The G(z)-alpha activity is inhibited by the phosphorylation and palmitoylation of the G-protein. Negatively regulates mu-opioid receptor-mediated activation of the G-proteins (By similarity).By similarity

GO - Molecular functioni

  1. GTPase activator activity Source: RefGenome

GO - Biological processi

  1. positive regulation of GTPase activity Source: GOC
  2. regulation of G-protein coupled receptor protein signaling pathway Source: ProtInc
  3. termination of G-protein coupled receptor signaling pathway Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Signal transduction inhibitor

Enzyme and pathway databases

ReactomeiREACT_19231. G alpha (i) signalling events.
REACT_19333. G alpha (z) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Regulator of G-protein signaling 20
Short name:
RGS20
Alternative name(s):
Gz-selective GTPase-activating protein
Short name:
G(z)GAP
Short name:
Gz-GAP
Regulator of G-protein signaling Z1
Regulator of Gz-selective protein signaling 1
Gene namesi
Name:RGS20
Synonyms:RGSZ1, ZGAP1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 8

Organism-specific databases

HGNCiHGNC:14600. RGS20.

Subcellular locationi

Membrane; Lipid-anchor. Nucleus. Cytoplasm
Note: Shuttles between the cytoplasm/cell membrane and the nucleus. Anchored to the membrane through palmitoylation.By similarity

GO - Cellular componenti

  1. cytoplasm Source: RefGenome
  2. nucleus Source: UniProtKB-KW
  3. plasma membrane Source: RefGenome
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA34373.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 388388Regulator of G-protein signaling 20PRO_0000204233Add
BLAST

Post-translational modificationi

Fatty acylated. Heavily palmitoylated in the cysteine string motif (By similarity).By similarity
N- and O-glycosylated in synapsomal membranes.By similarity
Serine phosphorylated in synapsomal membranes.By similarity
Sumoylated with SUMO1 and SUMO2 in synaptosomes. The sumoylated forms act as a scaffold for sequestering mu-opioid receptor-activated G(alpha) subunits (By similarity).By similarity

Keywords - PTMi

Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiO76081.
PaxDbiO76081.
PRIDEiO76081.

PTM databases

PhosphoSiteiO76081.

Expressioni

Tissue specificityi

Isoform 5 is expressed in brain at high levels in the caudate nucleus and temporal lobe.

Gene expression databases

BgeeiO76081.
CleanExiHS_RGS20.
ExpressionAtlasiO76081. baseline and differential.
GenevestigatoriO76081.

Interactioni

Subunit structurei

Forms a complex with G(alpha)z/i2 subunits and mu-opioid receptors; the formation of this complex results in mu-opioid receptor desensitization. Interacts with OPRM1 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Hoxa1P090223EBI-1052678,EBI-3957603From a different organism.
MTNR1AP480396EBI-1052678,EBI-1188238
MTNR1BP492862EBI-1052678,EBI-1188341

Protein-protein interaction databases

BioGridi114161. 22 interactions.
IntActiO76081. 8 interactions.
MINTiMINT-3002244.
STRINGi9606.ENSP00000297313.

Structurei

3D structure databases

ProteinModelPortaliO76081.
SMRiO76081. Positions 251-378.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini262 – 378117RGSPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi208 – 22013Poly-CysAdd
BLAST

Sequence similaritiesi

Contains 1 RGS domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG258376.
GeneTreeiENSGT00760000118903.
HOGENOMiHOG000233513.
HOVERGENiHBG013233.
InParanoidiO76081.
KOiK16449.
OMAiRFMNSAV.
OrthoDBiEOG7SN8DQ.
PhylomeDBiO76081.
TreeFamiTF315837.

Family and domain databases

Gene3Di1.10.196.10. 1 hit.
InterProiIPR024066. Regulat_G_prot_signal_dom1.
IPR016137. Regulat_G_prot_signal_superfam.
IPR000342. RGS_dom.
[Graphical view]
PfamiPF00615. RGS. 1 hit.
[Graphical view]
PRINTSiPR01301. RGSPROTEIN.
SMARTiSM00315. RGS. 1 hit.
[Graphical view]
SUPFAMiSSF48097. SSF48097. 1 hit.
PROSITEiPS50132. RGS. 1 hit.
[Graphical view]

Sequences (6)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative splicing. Align

Isoform 6 (identifier: O76081-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPQLSQDNQE CLQKHFSRPS IWTQFLPLFR AQRYNTDIHQ ITENEGDLRA
60 70 80 90 100
VPDIKSFPPA QLPDSPAAPK LFGLLSSPLS SLARFFSHLL RRPPPEAPRR
110 120 130 140 150
RLDFSPLLPA LPAARLSRGH EELPGRLSLL LGAALALPGR PSGGRPLRPP
160 170 180 190 200
HPVAKPREED ATAGQSSPMP QMGSERMEMR KRQMPAAQDT PGAAPGQPGA
210 220 230 240 250
GSRGSNACCF CWCCCCSCSC LTVRNQEDQR PTIASHELRA DLPTWEESPA
260 270 280 290 300
PTLEEVNAWA QSFDKLMVTP AGRNAFREFL RTEFSEENML FWMACEELKK
310 320 330 340 350
EANKNIIEEK ARIIYEDYIS ILSPKEVSLD SRVREVINRN MVEPSQHIFD
360 370 380
DAQLQIYTLM HRDSYPRFMN SAVYKDLLQS LSEKSIEA
Length:388
Mass (Da):43,692
Last modified:October 18, 2001 - v4
Checksum:iF44796D271F1765F
GO
Isoform 1 (identifier: O76081-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     56-170: Missing.

Show »
Length:273
Mass (Da):31,486
Checksum:i8BF212DBAF70A97E
GO
Isoform 2 (identifier: O76081-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-208: Missing.
     209-220: CFCWCCCCSCSC → MKETSGLFLISS

Show »
Length:180
Mass (Da):21,010
Checksum:iA2F8633711953F26
GO
Isoform 3 (identifier: O76081-4) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-236: Missing.
     237-247: ELRADLPTWEE → MKETSGLFLIS

Show »
Length:152
Mass (Da):17,735
Checksum:iE0554569F8C0CEA7
GO
Isoform 4 (identifier: O76081-5) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     3-55: Missing.
     56-170: Missing.

Show »
Length:220
Mass (Da):25,177
Checksum:iB5EA61FF181DA67C
GO
Isoform 5 (identifier: O76081-6) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-55: MPQLSQDNQE...GDLRAVPDIK → MRTADGGEPAGASSPAGRVDGGL
     56-170: Missing.

Show »
Length:241
Mass (Da):27,060
Checksum:i776410A4D698D622
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 236236Missing in isoform 3. 1 PublicationVSP_005698Add
BLAST
Alternative sequencei1 – 208208Missing in isoform 2. 1 PublicationVSP_005697Add
BLAST
Alternative sequencei1 – 5555MPQLS…VPDIK → MRTADGGEPAGASSPAGRVD GGL in isoform 5. 2 PublicationsVSP_005694Add
BLAST
Alternative sequencei3 – 5553Missing in isoform 4. 2 PublicationsVSP_005695Add
BLAST
Alternative sequencei56 – 170115Missing in isoform 1, isoform 4 and isoform 5. 5 PublicationsVSP_005696Add
BLAST
Alternative sequencei209 – 22012CFCWC…CSCSC → MKETSGLFLISS in isoform 2. 1 PublicationVSP_005699Add
BLAST
Alternative sequencei237 – 24711ELRADLPTWEE → MKETSGLFLIS in isoform 3. 1 PublicationVSP_005700Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF060877 mRNA. Translation: AAC62009.2.
AF366054 mRNA. Translation: AAK54122.1.
AF366055 mRNA. Translation: AAK54123.1.
AF366056 mRNA. Translation: AAK54124.1.
AF366057 mRNA. Translation: AAK54125.1.
AF074979 mRNA. Translation: AAC62013.1.
AF493940 mRNA. Translation: AAM12654.1.
BC015614 mRNA. Translation: AAH15614.2.
BC063490 mRNA. Translation: AAH63490.1.
AY046538 mRNA. Translation: AAL03971.1.
CCDSiCCDS6155.1. [O76081-1]
CCDS6156.1. [O76081-6]
CCDS69482.1. [O76081-2]
RefSeqiNP_001273602.1. NM_001286673.1. [O76081-2]
NP_001273603.1. NM_001286674.1. [O76081-4]
NP_001273604.1. NM_001286675.1. [O76081-3]
NP_003693.2. NM_003702.4. [O76081-6]
NP_733466.1. NM_170587.3. [O76081-1]
UniGeneiHs.368733.

Genome annotation databases

EnsembliENST00000276500; ENSP00000276500; ENSG00000147509. [O76081-6]
ENST00000297313; ENSP00000297313; ENSG00000147509. [O76081-1]
ENST00000344277; ENSP00000344630; ENSG00000147509. [O76081-2]
GeneIDi8601.
KEGGihsa:8601.
UCSCiuc003xrp.3. human. [O76081-1]
uc003xrq.3. human. [O76081-2]
uc010lye.3. human. [O76081-3]
uc010lyf.3. human. [O76081-4]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF060877 mRNA. Translation: AAC62009.2 .
AF366054 mRNA. Translation: AAK54122.1 .
AF366055 mRNA. Translation: AAK54123.1 .
AF366056 mRNA. Translation: AAK54124.1 .
AF366057 mRNA. Translation: AAK54125.1 .
AF074979 mRNA. Translation: AAC62013.1 .
AF493940 mRNA. Translation: AAM12654.1 .
BC015614 mRNA. Translation: AAH15614.2 .
BC063490 mRNA. Translation: AAH63490.1 .
AY046538 mRNA. Translation: AAL03971.1 .
CCDSi CCDS6155.1. [O76081-1 ]
CCDS6156.1. [O76081-6 ]
CCDS69482.1. [O76081-2 ]
RefSeqi NP_001273602.1. NM_001286673.1. [O76081-2 ]
NP_001273603.1. NM_001286674.1. [O76081-4 ]
NP_001273604.1. NM_001286675.1. [O76081-3 ]
NP_003693.2. NM_003702.4. [O76081-6 ]
NP_733466.1. NM_170587.3. [O76081-1 ]
UniGenei Hs.368733.

3D structure databases

ProteinModelPortali O76081.
SMRi O76081. Positions 251-378.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 114161. 22 interactions.
IntActi O76081. 8 interactions.
MINTi MINT-3002244.
STRINGi 9606.ENSP00000297313.

PTM databases

PhosphoSitei O76081.

Proteomic databases

MaxQBi O76081.
PaxDbi O76081.
PRIDEi O76081.

Protocols and materials databases

DNASUi 8601.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000276500 ; ENSP00000276500 ; ENSG00000147509 . [O76081-6 ]
ENST00000297313 ; ENSP00000297313 ; ENSG00000147509 . [O76081-1 ]
ENST00000344277 ; ENSP00000344630 ; ENSG00000147509 . [O76081-2 ]
GeneIDi 8601.
KEGGi hsa:8601.
UCSCi uc003xrp.3. human. [O76081-1 ]
uc003xrq.3. human. [O76081-2 ]
uc010lye.3. human. [O76081-3 ]
uc010lyf.3. human. [O76081-4 ]

Organism-specific databases

CTDi 8601.
GeneCardsi GC08P054764.
HGNCi HGNC:14600. RGS20.
MIMi 607193. gene.
neXtProti NX_O76081.
PharmGKBi PA34373.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG258376.
GeneTreei ENSGT00760000118903.
HOGENOMi HOG000233513.
HOVERGENi HBG013233.
InParanoidi O76081.
KOi K16449.
OMAi RFMNSAV.
OrthoDBi EOG7SN8DQ.
PhylomeDBi O76081.
TreeFami TF315837.

Enzyme and pathway databases

Reactomei REACT_19231. G alpha (i) signalling events.
REACT_19333. G alpha (z) signalling events.

Miscellaneous databases

GeneWikii RGS20.
GenomeRNAii 8601.
NextBioi 32227.
PROi O76081.
SOURCEi Search...

Gene expression databases

Bgeei O76081.
CleanExi HS_RGS20.
ExpressionAtlasi O76081. baseline and differential.
Genevestigatori O76081.

Family and domain databases

Gene3Di 1.10.196.10. 1 hit.
InterProi IPR024066. Regulat_G_prot_signal_dom1.
IPR016137. Regulat_G_prot_signal_superfam.
IPR000342. RGS_dom.
[Graphical view ]
Pfami PF00615. RGS. 1 hit.
[Graphical view ]
PRINTSi PR01301. RGSPROTEIN.
SMARTi SM00315. RGS. 1 hit.
[Graphical view ]
SUPFAMi SSF48097. SSF48097. 1 hit.
PROSITEi PS50132. RGS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "RGSZ1, a Gz-selective RGS protein in brain. Structure, membrane association, regulation by Galphaz phosphorylation, and relationship to a Gz GTPase-activating protein subfamily."
    Wang J., Ducret A., Tu Y., Kozasa T., Aebersold R., Ross E.M.
    J. Biol. Chem. 273:26014-26025(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 5), CHARACTERIZATION.
    Tissue: Fetal brain.
  2. Wang J., Ducret A., Ross E.M.
    Submitted (APR-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO N-TERMINUS.
  3. "RGSZ1 and Ret RGS: two of several splice variants from the gene RGS20."
    Barker S.A., Wang J., Sierra D.A., Ross E.M.
    Genomics 78:223-229(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2; 3; 4 AND 6).
    Tissue: Retina.
  4. "cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
    Puhl H.L. III, Ikeda S.R., Aronstam R.S.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 5).
    Tissue: Fetal brain and Skin.
  6. "RGSZ1, a Gz-selective regulator of G protein signaling whose action is sensitive to the phosphorylation state of Gzalpha."
    Glick J.L., Meigs T.E., Miron A., Casey P.J.
    J. Biol. Chem. 273:26008-26013(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 57-388 (ISOFORM 1).
  7. "Regulator of G protein signaling Z1 (RGSZ1) interacts with Galpha i subunits and regulates Galpha i-mediated cell signaling."
    Wang Y., Ho G., Zhang J.J., Nieuwenhuijsen B., Edris W., Chanda P.K., Young K.H.
    J. Biol. Chem. 277:48325-48332(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiRGS20_HUMAN
AccessioniPrimary (citable) accession number: O76081
Secondary accession number(s): Q96BG9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: October 18, 2001
Last modified: October 29, 2014
This is version 134 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3