O76076 (WISP2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
WNT1-inducible-signaling pathway protein 2
Short name=WISP-2

Alternative name(s):
CCN family member 5
Connective tissue growth factor-like protein
Short name=CTGF-L
Connective tissue growth factor-related protein 58

Gene names

Name:	WISP2
Synonyms:	CCN5, CT58, CTGFL
ORF Names:	UNQ228/PRO261

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	250 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play an important role in modulating bone turnover. Promotes the adhesion of osteoblast cells and inhibits the binding of fibrinogen to integrin receptors. In addition, inhibits osteocalcin production.
Subcellular location	Secreted Probable.
Tissue specificity	Expressed in primary osteoblasts, fibroblasts, ovary, testes, and heart.
Sequence similarities	Belongs to the CCN family. Contains 1 IGFBP N-terminal domain. Contains 1 TSP type-1 domain. Contains 1 VWFC domain.

Ontologies

Keywords
Biological process	Cell adhesion
Cellular component	Secreted
Coding sequence diversity	Polymorphism
Domain	Signal
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cell adhesion Inferred from electronic annotation. Source: UniProtKB-KW cell-cell signaling Traceable author statement Ref.3. Source: ProtInc regulation of cell growth Inferred from electronic annotation. Source: Compara signal transduction Traceable author statement Ref.3. Source: ProtInc
Cellular_component	extracellular space Inferred from direct assay Ref.1. Source: UniProtKB plasma membrane Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Ref.9
	Chain	24 – 250	227	WNT1-inducible-signaling pathway protein 2		PRO_0000014409
Regions
	Domain	24 – 94	71	IGFBP N-terminal
	Domain	98 – 164	67	VWFC
	Domain	194 – 238	45	TSP type-1
Natural variations
	Natural variant	59	1	R → Q. Corresponds to variant rs33932543 [ dbSNP | Ensembl ].		VAR_049566

Sequences

	Sequence		Length	Mass (Da)
	O76076 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: C499837EF42FEEAC	FASTA	250	26,825
	10 20 30 40 50 60 MRGTPKTHLL AFSLLCLLSK VRTQLCPTPC TCPWPPPRCP LGVPLVLDGC GCCRVCARRL 70 80 90 100 110 120 GEPCDQLHVC DASQGLVCQP GAGPGGRGAL CLLAEDDSSC EVNGRLYREG ETFQPHCSIR 130 140 150 160 170 180 CRCEDGGFTC VPLCSEDVRL PSWDCPHPRR VEVLGKCCPE WVCGQGGGLG TQPLPAQGPQ 190 200 210 220 230 240 FSGLVSSLPP GVPCPEWSTA WGPCSTTCGL GMATRVSNQN RFCRLETQRR LCLSRPCPPS 250 RGRSPQNSAF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and cloning of a connective tissue growth factor-like cDNA from human osteoblasts encoding a novel regulator of osteoblast functions." Kumar S., Hand A.T., Connor J.R., Dodds R.A., Ryan P.J., Trill J.J., Fisher S.M., Nuttall M.E., Lipshutz D.B., Zou C., Hwang S.M., Votta B.J., James I.E., Rieman D.J., Gowen M., Lee J.C. J. Biol. Chem. 274:17123-17131(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"CT58, a new member of the connective tissue growth factor family, interacts with the breast cancer associated mucin MUC1." Rowles J., Gendler S. Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"WISP genes are members of the connective tissue growth factor family that are up-regulated in wnt-1-transformed cells and aberrantly expressed in human colon tumors." Pennica D., Swanson T.A., Welsh J.W., Roy M.A., Lawrence D.A., Lee J., Brush J., Taneyhill L.A., Deuel B., Lew M., Watanabe C., Cohen R.L., Melham M.F., Finley G.G., Quirke P., Goddard A.D., Hillan K.J., Gurney A.L., Botstein D., Levine A.J. Proc. Natl. Acad. Sci. U.S.A. 95:14717-14722(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Lung.
[4]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Skeletal muscle.
[6]	"The DNA sequence and comparative analysis of human chromosome 20." Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J. Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung and Ovary.
[9]	"Signal peptide prediction based on analysis of experimentally verified cleavage sites." Zhang Z., Henzel W.J. Protein Sci. 13:2819-2824(2004) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 24-38.
+	Additional computationally mapped references.

Web resources

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF083500 mRNA. Translation: AAC70350.1. AF074604 mRNA. Translation: AAC26794.1. AF100780 mRNA. Translation: AAC96322.1. AY358915 mRNA. Translation: AAQ89274.1. AK313853 mRNA. Translation: BAG36581.1. AL139352 Genomic DNA. Translation: CAB94788.1. CH471077 Genomic DNA. Translation: EAW75902.1. CH471077 Genomic DNA. Translation: EAW75903.1. BC017782 mRNA. Translation: AAH17782.1. BC064379 mRNA. Translation: AAH64379.1.
IPI	IPI00022052.
RefSeq	NP_003872.1. NM_003881.2.
UniGene	Hs.592145.
3D structure databases
ProteinModelPortal	O76076.
ModBase	Search...
Protein-protein interaction databases
IntAct	O76076. 2 interactions.
STRING	9606.ENSP00000190983.
Proteomic databases
PaxDb	O76076.
PeptideAtlas	O76076.
PRIDE	O76076.
Protocols and materials databases
DNASU	8839.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000190983; ENSP00000190983; ENSG00000064205. ENST00000372868; ENSP00000361959; ENSG00000064205.
GeneID	8839.
KEGG	hsa:8839.
UCSC	uc002xmp.3. human.
Organism-specific databases
CTD	8839.
GeneCards	GC20P043343.
HGNC	HGNC:12770. WISP2.
HPA	CAB019273.
MIM	603399. gene.
neXtProt	NX_O76076.
PharmGKB	PA37373.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG82184.
HOGENOM	HOG000231462.
HOVERGEN	HBG000635.
InParanoid	O76076.
OMA	PHCRIRC.
OrthoDB	EOG4XPQGQ.
PhylomeDB	O76076.
Gene expression databases
ArrayExpress	O76076.
Bgee	O76076.
CleanEx	HS_WISP2.
Genevestigator	O76076.
GermOnline	ENSG00000064205. Homo sapiens.
Family and domain databases
InterPro	IPR000867. IGFBP-like. IPR017891. Insulin_GF-bd_Cys-rich_CS. IPR000884. Thrombospondin_1_rpt. IPR001007. VWF_C. [Graphical view]
Pfam	PF00219. IGFBP. 1 hit. PF00090. TSP_1. 1 hit. PF00093. VWC. 1 hit. [Graphical view]
SMART	SM00121. IB. 1 hit. SM00209. TSP1. 1 hit. SM00214. VWC. 1 hit. [Graphical view]
SUPFAM	SSF82895. TSP1. 1 hit.
PROSITE	PS00222. IGFBP_N_1. 1 hit. PS51323. IGFBP_N_2. 1 hit. PS50092. TSP1. 1 hit. PS01208. VWFC_1. 1 hit. PS50184. VWFC_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	8839.
NextBio	33180.
SOURCE	Search...

Entry information

Entry name

WISP2_HUMAN

Accession

Primary (citable) accession number: O76076
Secondary accession number(s): B2R9N4, E1P612

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 19, 2002
Last sequence update:	November 1, 1998
Last modified:	May 1, 2013
This is version 109 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.