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Protein

WNT1-inducible-signaling pathway protein 2

Gene

WISP2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play an important role in modulating bone turnover. Promotes the adhesion of osteoblast cells and inhibits the binding of fibrinogen to integrin receptors. In addition, inhibits osteocalcin production.

GO - Molecular functioni

GO - Biological processi

  • cell adhesion Source: GO_Central
  • cell-cell signaling Source: ProtInc
  • negative regulation of cell death Source: GO_Central
  • regulation of cell growth Source: Ensembl
  • signal transduction Source: ProtInc
Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Enzyme and pathway databases

SignaLinkiO76076.

Names & Taxonomyi

Protein namesi
Recommended name:
WNT1-inducible-signaling pathway protein 2
Short name:
WISP-2
Alternative name(s):
CCN family member 5
Connective tissue growth factor-like protein
Short name:
CTGF-L
Connective tissue growth factor-related protein 58
Gene namesi
Name:WISP2
Synonyms:CCN5, CT58, CTGFL
ORF Names:UNQ228/PRO261
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 20

Organism-specific databases

HGNCiHGNC:12770. WISP2.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: UniProtKB
  • extracellular space Source: UniProtKB
  • plasma membrane Source: Ensembl
  • proteinaceous extracellular matrix Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA37373.

Polymorphism and mutation databases

BioMutaiWISP2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 23231 PublicationAdd
BLAST
Chaini24 – 250227WNT1-inducible-signaling pathway protein 2PRO_0000014409Add
BLAST

Proteomic databases

PaxDbiO76076.
PeptideAtlasiO76076.
PRIDEiO76076.

Expressioni

Tissue specificityi

Expressed in primary osteoblasts, fibroblasts, ovary, testes, and heart.

Gene expression databases

BgeeiO76076.
CleanExiHS_WISP2.
GenevisibleiO76076. HS.

Organism-specific databases

HPAiCAB019273.

Interactioni

Protein-protein interaction databases

BioGridi114366. 5 interactions.
IntActiO76076. 4 interactions.
STRINGi9606.ENSP00000190983.

Structurei

3D structure databases

ProteinModelPortaliO76076.
SMRiO76076. Positions 36-133.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini24 – 9471IGFBP N-terminalPROSITE-ProRule annotationAdd
BLAST
Domaini98 – 16467VWFCPROSITE-ProRule annotationAdd
BLAST
Domaini194 – 23845TSP type-1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the CCN family.Curated
Contains 1 IGFBP N-terminal domain.PROSITE-ProRule annotation
Contains 1 TSP type-1 domain.PROSITE-ProRule annotation
Contains 1 VWFC domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG82184.
GeneTreeiENSGT00760000119225.
HOGENOMiHOG000231462.
HOVERGENiHBG000635.
InParanoidiO76076.
OMAiSNQNRFC.
OrthoDBiEOG77T15K.
PhylomeDBiO76076.
TreeFamiTF326070.

Family and domain databases

InterProiIPR009030. Growth_fac_rcpt_N_dom.
IPR000867. IGFBP-like.
IPR017891. Insulin_GF-bd_Cys-rich_CS.
IPR000884. Thrombospondin_1_rpt.
IPR001007. VWF_C.
[Graphical view]
PfamiPF00219. IGFBP. 1 hit.
PF00090. TSP_1. 1 hit.
PF00093. VWC. 1 hit.
[Graphical view]
SMARTiSM00121. IB. 1 hit.
SM00209. TSP1. 1 hit.
SM00214. VWC. 1 hit.
[Graphical view]
SUPFAMiSSF57184. SSF57184. 1 hit.
SSF82895. SSF82895. 1 hit.
PROSITEiPS00222. IGFBP_N_1. 1 hit.
PS51323. IGFBP_N_2. 1 hit.
PS50092. TSP1. 1 hit.
PS01208. VWFC_1. 1 hit.
PS50184. VWFC_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O76076-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRGTPKTHLL AFSLLCLLSK VRTQLCPTPC TCPWPPPRCP LGVPLVLDGC
60 70 80 90 100
GCCRVCARRL GEPCDQLHVC DASQGLVCQP GAGPGGRGAL CLLAEDDSSC
110 120 130 140 150
EVNGRLYREG ETFQPHCSIR CRCEDGGFTC VPLCSEDVRL PSWDCPHPRR
160 170 180 190 200
VEVLGKCCPE WVCGQGGGLG TQPLPAQGPQ FSGLVSSLPP GVPCPEWSTA
210 220 230 240 250
WGPCSTTCGL GMATRVSNQN RFCRLETQRR LCLSRPCPPS RGRSPQNSAF
Length:250
Mass (Da):26,825
Last modified:November 1, 1998 - v1
Checksum:iC499837EF42FEEAC
GO
Isoform 2 (identifier: O76076-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     93-218: LAEDDSSCEV...GLGMATRVSN → CKQDPSFLAL...AWVHHAEHQY
     219-250: Missing.

Note: No experimental confirmation available.
Show »
Length:218
Mass (Da):22,577
Checksum:i2A8A79681111542B
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti59 – 591R → Q.
Corresponds to variant rs33932543 [ dbSNP | Ensembl ].
VAR_049566

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei93 – 218126LAEDD…TRVSN → CKQDPSFLALSLPCPLVSPA QNGARPGDPARPPVGWAWPP GCPTRTASADWRPSAACACP GPAHPPGVAVHKTVPSRAGL GMGTRCPPSPAGGPVPGPWA DGRWSVPRPLAAGNTLAWVH HAEHQY in isoform 2. 1 PublicationVSP_056298Add
BLAST
Alternative sequencei219 – 25032Missing in isoform 2. 1 PublicationVSP_056299Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF083500 mRNA. Translation: AAC70350.1.
AF074604 mRNA. Translation: AAC26794.1.
AF100780 mRNA. Translation: AAC96322.1.
AY358915 mRNA. Translation: AAQ89274.1.
AK313853 mRNA. Translation: BAG36581.1.
AL139352 Genomic DNA. Translation: CAB94788.1.
CH471077 Genomic DNA. Translation: EAW75902.1.
CH471077 Genomic DNA. Translation: EAW75903.1.
BC017782 mRNA. Translation: AAH17782.1.
BC058074 mRNA. Translation: AAH58074.1.
BC064379 mRNA. Translation: AAH64379.1.
CCDSiCCDS13336.1. [O76076-1]
RefSeqiNP_003872.1. NM_003881.2. [O76076-1]
XP_005260660.1. XM_005260603.3. [O76076-1]
UniGeneiHs.592145.

Genome annotation databases

EnsembliENST00000190983; ENSP00000190983; ENSG00000064205. [O76076-1]
ENST00000372865; ENSP00000361956; ENSG00000064205. [O76076-2]
ENST00000372868; ENSP00000361959; ENSG00000064205. [O76076-1]
GeneIDi8839.
KEGGihsa:8839.
UCSCiuc002xmp.3. human. [O76076-1]
uc002xmq.3. human.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF083500 mRNA. Translation: AAC70350.1.
AF074604 mRNA. Translation: AAC26794.1.
AF100780 mRNA. Translation: AAC96322.1.
AY358915 mRNA. Translation: AAQ89274.1.
AK313853 mRNA. Translation: BAG36581.1.
AL139352 Genomic DNA. Translation: CAB94788.1.
CH471077 Genomic DNA. Translation: EAW75902.1.
CH471077 Genomic DNA. Translation: EAW75903.1.
BC017782 mRNA. Translation: AAH17782.1.
BC058074 mRNA. Translation: AAH58074.1.
BC064379 mRNA. Translation: AAH64379.1.
CCDSiCCDS13336.1. [O76076-1]
RefSeqiNP_003872.1. NM_003881.2. [O76076-1]
XP_005260660.1. XM_005260603.3. [O76076-1]
UniGeneiHs.592145.

3D structure databases

ProteinModelPortaliO76076.
SMRiO76076. Positions 36-133.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114366. 5 interactions.
IntActiO76076. 4 interactions.
STRINGi9606.ENSP00000190983.

Polymorphism and mutation databases

BioMutaiWISP2.

Proteomic databases

PaxDbiO76076.
PeptideAtlasiO76076.
PRIDEiO76076.

Protocols and materials databases

DNASUi8839.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000190983; ENSP00000190983; ENSG00000064205. [O76076-1]
ENST00000372865; ENSP00000361956; ENSG00000064205. [O76076-2]
ENST00000372868; ENSP00000361959; ENSG00000064205. [O76076-1]
GeneIDi8839.
KEGGihsa:8839.
UCSCiuc002xmp.3. human. [O76076-1]
uc002xmq.3. human.

Organism-specific databases

CTDi8839.
GeneCardsiGC20P043343.
HGNCiHGNC:12770. WISP2.
HPAiCAB019273.
MIMi603399. gene.
neXtProtiNX_O76076.
PharmGKBiPA37373.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG82184.
GeneTreeiENSGT00760000119225.
HOGENOMiHOG000231462.
HOVERGENiHBG000635.
InParanoidiO76076.
OMAiSNQNRFC.
OrthoDBiEOG77T15K.
PhylomeDBiO76076.
TreeFamiTF326070.

Enzyme and pathway databases

SignaLinkiO76076.

Miscellaneous databases

GeneWikiiWNT1-inducible-signaling_pathway_protein_2.
GenomeRNAii8839.
NextBioi33180.
PROiO76076.
SOURCEiSearch...

Gene expression databases

BgeeiO76076.
CleanExiHS_WISP2.
GenevisibleiO76076. HS.

Family and domain databases

InterProiIPR009030. Growth_fac_rcpt_N_dom.
IPR000867. IGFBP-like.
IPR017891. Insulin_GF-bd_Cys-rich_CS.
IPR000884. Thrombospondin_1_rpt.
IPR001007. VWF_C.
[Graphical view]
PfamiPF00219. IGFBP. 1 hit.
PF00090. TSP_1. 1 hit.
PF00093. VWC. 1 hit.
[Graphical view]
SMARTiSM00121. IB. 1 hit.
SM00209. TSP1. 1 hit.
SM00214. VWC. 1 hit.
[Graphical view]
SUPFAMiSSF57184. SSF57184. 1 hit.
SSF82895. SSF82895. 1 hit.
PROSITEiPS00222. IGFBP_N_1. 1 hit.
PS51323. IGFBP_N_2. 1 hit.
PS50092. TSP1. 1 hit.
PS01208. VWFC_1. 1 hit.
PS50184. VWFC_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and cloning of a connective tissue growth factor-like cDNA from human osteoblasts encoding a novel regulator of osteoblast functions."
    Kumar S., Hand A.T., Connor J.R., Dodds R.A., Ryan P.J., Trill J.J., Fisher S.M., Nuttall M.E., Lipshutz D.B., Zou C., Hwang S.M., Votta B.J., James I.E., Rieman D.J., Gowen M., Lee J.C.
    J. Biol. Chem. 274:17123-17131(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "CT58, a new member of the connective tissue growth factor family, interacts with the breast cancer associated mucin MUC1."
    Rowles J., Gendler S.
    Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  3. "WISP genes are members of the connective tissue growth factor family that are up-regulated in wnt-1-transformed cells and aberrantly expressed in human colon tumors."
    Pennica D., Swanson T.A., Welsh J.W., Roy M.A., Lawrence D.A., Lee J., Brush J., Taneyhill L.A., Deuel B., Lew M., Watanabe C., Cohen R.L., Melham M.F., Finley G.G., Quirke P., Goddard A.D., Hillan K.J., Gurney A.L., Botstein D., Levine A.J.
    Proc. Natl. Acad. Sci. U.S.A. 95:14717-14722(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Lung.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  5. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Skeletal muscle.
  6. "The DNA sequence and comparative analysis of human chromosome 20."
    Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E.
    , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.
    Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  8. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Brain, Lung and Ovary.
  9. "Signal peptide prediction based on analysis of experimentally verified cleavage sites."
    Zhang Z., Henzel W.J.
    Protein Sci. 13:2819-2824(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 24-38.

Entry informationi

Entry nameiWISP2_HUMAN
AccessioniPrimary (citable) accession number: O76076
Secondary accession number(s): B2R9N4, E1P612, Q6PEG3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 19, 2002
Last sequence update: November 1, 1998
Last modified: June 24, 2015
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.