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O76074

- PDE5A_HUMAN

UniProt

O76074 - PDE5A_HUMAN

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Protein
cGMP-specific 3',5'-cyclic phosphodiesterase
Gene
PDE5A, PDE5
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase catalyzes the specific hydrolysis of cGMP to 5'-GMP.

Catalytic activityi

Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate.

Cofactori

Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.

Enzyme regulationi

Sildenafil (Viagra) is a highly selective and potent inhibitor of PDE5A and is effective in the treatment of penile erectile dysfunction. Also inhibited by zaprinast.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei613 – 6131Proton donor By similarity
Metal bindingi617 – 6171Divalent metal cation 1
Metal bindingi653 – 6531Divalent metal cation 1
Metal bindingi654 – 6541Divalent metal cation 1
Metal bindingi654 – 6541Divalent metal cation 2
Metal bindingi764 – 7641Divalent metal cation 1
Binding sitei817 – 8171cGMP

GO - Molecular functioni

  1. 3',5'-cyclic-GMP phosphodiesterase activity Source: UniProtKB-EC
  2. 3',5'-cyclic-nucleotide phosphodiesterase activity Source: UniProtKB
  3. cGMP binding Source: UniProtKB
  4. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. blood coagulation Source: Reactome
  2. cGMP catabolic process Source: UniProtKB
  3. negative regulation of T cell proliferation Source: Ensembl
  4. negative regulation of cardiac muscle contraction Source: Ensembl
  5. positive regulation of MAP kinase activity Source: Ensembl
  6. positive regulation of cardiac muscle hypertrophy Source: Ensembl
  7. positive regulation of oocyte development Source: Ensembl
  8. relaxation of cardiac muscle Source: Ensembl
  9. signal transduction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

cGMP, cGMP-binding, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi3.1.4.35. 2681.
ReactomeiREACT_23767. cGMP effects.
UniPathwayiUPA00763; UER00748.

Names & Taxonomyi

Protein namesi
Recommended name:
cGMP-specific 3',5'-cyclic phosphodiesterase (EC:3.1.4.35)
Alternative name(s):
cGMP-binding cGMP-specific phosphodiesterase
Short name:
CGB-PDE
Gene namesi
Name:PDE5A
Synonyms:PDE5
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 4

Organism-specific databases

HGNCiHGNC:8784. PDE5A.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: Reactome
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA33132.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 875875cGMP-specific 3',5'-cyclic phosphodiesterase
PRO_0000198823Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei102 – 1021Phosphoserine Reviewed prediction

Post-translational modificationi

Phosphorylation is regulated by binding of cGMP to the two allosteric sites By similarity. Phosphorylation by PRKG1 leads to its activation.

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO76074.
PaxDbiO76074.
PRIDEiO76074.

PTM databases

PhosphoSiteiO76074.

Expressioni

Tissue specificityi

Expressed in aortic smooth muscle cells, heart, placenta, skeletal muscle and pancreas and, to a much lesser extent, in brain, liver and lung.

Gene expression databases

ArrayExpressiO76074.
BgeeiO76074.
CleanExiHS_PDE5A.
GenevestigatoriO76074.

Organism-specific databases

HPAiHPA004729.
HPA012873.

Interactioni

Protein-protein interaction databases

BioGridi114205. 1 interaction.
DIPiDIP-46287N.
STRINGi9606.ENSP00000347046.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi103 – 1064
Beta strandi115 – 1173
Turni118 – 1203
Beta strandi121 – 1244
Helixi148 – 16013
Helixi165 – 17713
Beta strandi182 – 19211
Beta strandi198 – 2058
Beta strandi208 – 2103
Beta strandi213 – 2186
Beta strandi221 – 2244
Helixi228 – 2369
Beta strandi240 – 2434
Helixi245 – 2473
Helixi254 – 2596
Beta strandi266 – 2727
Beta strandi278 – 28710
Helixi299 – 34042
Turni342 – 3454
Helixi346 – 36217
Beta strandi365 – 3728
Beta strandi374 – 3763
Beta strandi379 – 3879
Turni388 – 3903
Beta strandi391 – 3933
Helixi400 – 4034
Helixi407 – 4093
Helixi410 – 4189
Beta strandi422 – 4254
Turni427 – 4293
Beta strandi431 – 4333
Beta strandi451 – 4577
Beta strandi461 – 47111
Turni476 – 4783
Helixi486 – 50621
Helixi535 – 54511
Helixi551 – 5544
Turni555 – 5573
Helixi568 – 58114
Helixi584 – 5874
Helixi592 – 60413
Turni606 – 6083
Beta strandi610 – 6145
Helixi615 – 63016
Turni631 – 6333
Helixi635 – 6373
Helixi640 – 65213
Turni653 – 6564
Helixi662 – 6676
Helixi671 – 6755
Beta strandi677 – 6793
Helixi680 – 69415
Turni700 – 7034
Helixi706 – 72217
Helixi725 – 74016
Beta strandi746 – 7483
Helixi749 – 76416
Helixi766 – 7694
Helixi772 – 79625
Helixi803 – 8053
Helixi807 – 8126
Helixi813 – 82311
Helixi825 – 83511
Helixi837 – 8393
Helixi840 – 85718

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1RKPX-ray2.05A535-860[»]
1T9RX-ray2.10A531-875[»]
1T9SX-ray2.00A/B534-858[»]
1TBFX-ray1.30A534-858[»]
1UDTX-ray2.30A537-860[»]
1UDUX-ray2.83A/B537-860[»]
1UHOX-ray2.50A537-860[»]
1XOZX-ray1.37A534-875[»]
1XP0X-ray1.79A534-875[»]
2CHMX-ray1.60A534-656[»]
A682-858[»]
2H40X-ray1.85A535-860[»]
2H42X-ray2.30A/B/C535-860[»]
2H44X-ray1.80A535-860[»]
2XSSX-ray2.50A/B346-509[»]
3B2RX-ray2.07A/B535-860[»]
3BJCX-ray2.00A1-875[»]
3HC8X-ray1.79A536-858[»]
3HDZX-ray1.80A536-657[»]
A682-858[»]
3JWQX-ray2.87A/B/C/D535-786[»]
A/B/C/D827-860[»]
3JWRX-ray2.99A/B535-786[»]
A/B706-860[»]
3LFVX-ray2.80A/B98-518[»]
3MF0X-ray3.10A/B89-518[»]
3SHYX-ray2.65A535-860[»]
3SHZX-ray2.45A535-860[»]
3SIEX-ray1.93A/B535-860[»]
3TGEX-ray1.96A534-656[»]
A682-858[»]
3TGGX-ray1.91A534-858[»]
3TSEX-ray2.14A535-860[»]
3TSFX-ray2.44A535-860[»]
4G2WX-ray2.28A535-860[»]
4G2YX-ray2.40A535-860[»]
4I9ZX-ray2.08A535-860[»]
4IA0X-ray2.17A535-860[»]
ProteinModelPortaliO76074.
SMRiO76074. Positions 98-860.

Miscellaneous databases

EvolutionaryTraceiO76074.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini164 – 314151GAF 1
Add
BLAST
Domaini346 – 503158GAF 2
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni588 – 853266Catalytic By similarity
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi10 – 2415Poly-Gln
Add
BLAST

Domaini

Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain which contains two homologous allosteric cGMP-binding regions, A and B.

Sequence similaritiesi

Contains 2 GAF domains.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG270709.
HOVERGENiHBG101207.
InParanoidiO76074.
KOiK13762.
OMAiREHDANK.
OrthoDBiEOG7RRF69.
PhylomeDBiO76074.
TreeFamiTF316499.

Family and domain databases

Gene3Di1.10.1300.10. 1 hit.
3.30.450.40. 2 hits.
InterProiIPR003018. GAF.
IPR029016. GAF_dom_like.
IPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view]
PfamiPF01590. GAF. 2 hits.
PF00233. PDEase_I. 1 hit.
[Graphical view]
PRINTSiPR00387. PDIESTERASE1.
SMARTiSM00065. GAF. 2 hits.
SM00471. HDc. 1 hit.
[Graphical view]
SUPFAMiSSF55781. SSF55781. 2 hits.
PROSITEiPS00126. PDEASE_I. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform PDE5A1 (identifier: O76074-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MERAGPSFGQ QRQQQQPQQQ KQQQRDQDSV EAWLDDHWDF TFSYFVRKAT    50
REMVNAWFAE RVHTIPVCKE GIRGHTESCS CPLQQSPRAD NSAPGTPTRK 100
ISASEFDRPL RPIVVKDSEG TVSFLSDSEK KEQMPLTPPR FDHDEGDQCS 150
RLLELVKDIS SHLDVTALCH KIFLHIHGLI SADRYSLFLV CEDSSNDKFL 200
ISRLFDVAEG STLEEVSNNC IRLEWNKGIV GHVAALGEPL NIKDAYEDPR 250
FNAEVDQITG YKTQSILCMP IKNHREEVVG VAQAINKKSG NGGTFTEKDE 300
KDFAAYLAFC GIVLHNAQLY ETSLLENKRN QVLLDLASLI FEEQQSLEVI 350
LKKIAATIIS FMQVQKCTIF IVDEDCSDSF SSVFHMECEE LEKSSDTLTR 400
EHDANKINYM YAQYVKNTME PLNIPDVSKD KRFPWTTENT GNVNQQCIRS 450
LLCTPIKNGK KNKVIGVCQL VNKMEENTGK VKPFNRNDEQ FLEAFVIFCG 500
LGIQNTQMYE AVERAMAKQM VTLEVLSYHA SAAEEETREL QSLAAAVVPS 550
AQTLKITDFS FSDFELSDLE TALCTIRMFT DLNLVQNFQM KHEVLCRWIL 600
SVKKNYRKNV AYHNWRHAFN TAQCMFAALK AGKIQNKLTD LEILALLIAA 650
LSHDLDHRGV NNSYIQRSEH PLAQLYCHSI MEHHHFDQCL MILNSPGNQI 700
LSGLSIEEYK TTLKIIKQAI LATDLALYIK RRGEFFELIR KNQFNLEDPH 750
QKELFLAMLM TACDLSAITK PWPIQQRIAE LVATEFFDQG DRERKELNIE 800
PTDLMNREKK NKIPSMQVGF IDAICLQLYE ALTHVSEDCF PLLDGCRKNR 850
QKWQALAEQQ EKMLINGESG QAKRN 875
Length:875
Mass (Da):99,985
Last modified:January 11, 2011 - v2
Checksum:i9E30C6C182F13388
GO
Isoform PDE5A2 (identifier: O76074-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-49: MERAGPSFGQQRQQQQPQQQKQQQRDQDSVEAWLDDHWDFTFSYFVRKA → MLPFGDK

Show »
Length:833
Mass (Da):94,805
Checksum:i41D0B4B1BAB57D6D
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti93 – 931A → V.5 Publications
Corresponds to variant rs3733526 [ dbSNP | Ensembl ].
VAR_027775
Natural varianti181 – 1811S → A.
Corresponds to variant rs17051276 [ dbSNP | Ensembl ].
VAR_027776

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 4949MERAG…FVRKA → MLPFGDK in isoform PDE5A2.
VSP_004591Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti159 – 1591I → V in AAP31235. 1 Publication
Sequence conflicti310 – 3101C → G in AAP31235. 1 Publication
Sequence conflicti381 – 3811S → F in AAP31235. 1 Publication
Sequence conflicti406 – 4061K → R in AAP31235. 1 Publication
Sequence conflicti642 – 6421E → G in BAA81667. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF043731 mRNA. Translation: AAC63967.1.
AF043732 mRNA. Translation: AAC63968.1.
AB001635 Genomic DNA. Translation: BAA33372.2.
D89094 mRNA. Translation: BAA28945.1.
AJ004865 mRNA. Translation: CAA06170.1.
AB015656 mRNA. Translation: BAA81667.1.
AY264918 mRNA. Translation: AAP21809.1.
AK290189 mRNA. Translation: BAF82878.1.
AC093752 Genomic DNA. No translation available.
AC080089 Genomic DNA. No translation available.
BC126233 mRNA. Translation: AAI26234.1.
AY266363 mRNA. Translation: AAP31235.1.
CCDSiCCDS34055.1. [O76074-2]
CCDS3713.1. [O76074-1]
PIRiJW0106.
RefSeqiNP_001074.2. NM_001083.3. [O76074-1]
NP_236914.2. NM_033430.2. [O76074-2]
NP_246273.2. NM_033437.3.
UniGeneiHs.647971.

Genome annotation databases

EnsembliENST00000264805; ENSP00000264805; ENSG00000138735. [O76074-2]
ENST00000354960; ENSP00000347046; ENSG00000138735. [O76074-1]
GeneIDi8654.
KEGGihsa:8654.
UCSCiuc003idf.3. human. [O76074-2]
uc003idg.3. human. [O76074-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF043731 mRNA. Translation: AAC63967.1 .
AF043732 mRNA. Translation: AAC63968.1 .
AB001635 Genomic DNA. Translation: BAA33372.2 .
D89094 mRNA. Translation: BAA28945.1 .
AJ004865 mRNA. Translation: CAA06170.1 .
AB015656 mRNA. Translation: BAA81667.1 .
AY264918 mRNA. Translation: AAP21809.1 .
AK290189 mRNA. Translation: BAF82878.1 .
AC093752 Genomic DNA. No translation available.
AC080089 Genomic DNA. No translation available.
BC126233 mRNA. Translation: AAI26234.1 .
AY266363 mRNA. Translation: AAP31235.1 .
CCDSi CCDS34055.1. [O76074-2 ]
CCDS3713.1. [O76074-1 ]
PIRi JW0106.
RefSeqi NP_001074.2. NM_001083.3. [O76074-1 ]
NP_236914.2. NM_033430.2. [O76074-2 ]
NP_246273.2. NM_033437.3.
UniGenei Hs.647971.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1RKP X-ray 2.05 A 535-860 [» ]
1T9R X-ray 2.10 A 531-875 [» ]
1T9S X-ray 2.00 A/B 534-858 [» ]
1TBF X-ray 1.30 A 534-858 [» ]
1UDT X-ray 2.30 A 537-860 [» ]
1UDU X-ray 2.83 A/B 537-860 [» ]
1UHO X-ray 2.50 A 537-860 [» ]
1XOZ X-ray 1.37 A 534-875 [» ]
1XP0 X-ray 1.79 A 534-875 [» ]
2CHM X-ray 1.60 A 534-656 [» ]
A 682-858 [» ]
2H40 X-ray 1.85 A 535-860 [» ]
2H42 X-ray 2.30 A/B/C 535-860 [» ]
2H44 X-ray 1.80 A 535-860 [» ]
2XSS X-ray 2.50 A/B 346-509 [» ]
3B2R X-ray 2.07 A/B 535-860 [» ]
3BJC X-ray 2.00 A 1-875 [» ]
3HC8 X-ray 1.79 A 536-858 [» ]
3HDZ X-ray 1.80 A 536-657 [» ]
A 682-858 [» ]
3JWQ X-ray 2.87 A/B/C/D 535-786 [» ]
A/B/C/D 827-860 [» ]
3JWR X-ray 2.99 A/B 535-786 [» ]
A/B 706-860 [» ]
3LFV X-ray 2.80 A/B 98-518 [» ]
3MF0 X-ray 3.10 A/B 89-518 [» ]
3SHY X-ray 2.65 A 535-860 [» ]
3SHZ X-ray 2.45 A 535-860 [» ]
3SIE X-ray 1.93 A/B 535-860 [» ]
3TGE X-ray 1.96 A 534-656 [» ]
A 682-858 [» ]
3TGG X-ray 1.91 A 534-858 [» ]
3TSE X-ray 2.14 A 535-860 [» ]
3TSF X-ray 2.44 A 535-860 [» ]
4G2W X-ray 2.28 A 535-860 [» ]
4G2Y X-ray 2.40 A 535-860 [» ]
4I9Z X-ray 2.08 A 535-860 [» ]
4IA0 X-ray 2.17 A 535-860 [» ]
ProteinModelPortali O76074.
SMRi O76074. Positions 98-860.
ModBasei Search...

Protein-protein interaction databases

BioGridi 114205. 1 interaction.
DIPi DIP-46287N.
STRINGi 9606.ENSP00000347046.

Chemistry

BindingDBi O76074.
ChEMBLi CHEMBL2111340.
DrugBanki DB00975. Dipyridamole.
DB00806. Pentoxifylline.
DB00203. Sildenafil.
DB00820. Tadalafil.
DB00277. Theophylline.
DB00862. Vardenafil.
GuidetoPHARMACOLOGYi 1304.

PTM databases

PhosphoSitei O76074.

Proteomic databases

MaxQBi O76074.
PaxDbi O76074.
PRIDEi O76074.

Protocols and materials databases

DNASUi 8654.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000264805 ; ENSP00000264805 ; ENSG00000138735 . [O76074-2 ]
ENST00000354960 ; ENSP00000347046 ; ENSG00000138735 . [O76074-1 ]
GeneIDi 8654.
KEGGi hsa:8654.
UCSCi uc003idf.3. human. [O76074-2 ]
uc003idg.3. human. [O76074-1 ]

Organism-specific databases

CTDi 8654.
GeneCardsi GC04M120415.
HGNCi HGNC:8784. PDE5A.
HPAi HPA004729.
HPA012873.
MIMi 603310. gene.
neXtProti NX_O76074.
PharmGKBi PA33132.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG270709.
HOVERGENi HBG101207.
InParanoidi O76074.
KOi K13762.
OMAi REHDANK.
OrthoDBi EOG7RRF69.
PhylomeDBi O76074.
TreeFami TF316499.

Enzyme and pathway databases

UniPathwayi UPA00763 ; UER00748 .
BRENDAi 3.1.4.35. 2681.
Reactomei REACT_23767. cGMP effects.

Miscellaneous databases

ChiTaRSi PDE5A. human.
EvolutionaryTracei O76074.
GeneWikii CGMP-specific_phosphodiesterase_type_5.
GenomeRNAii 8654.
NextBioi 32453.
PROi O76074.
SOURCEi Search...

Gene expression databases

ArrayExpressi O76074.
Bgeei O76074.
CleanExi HS_PDE5A.
Genevestigatori O76074.

Family and domain databases

Gene3Di 1.10.1300.10. 1 hit.
3.30.450.40. 2 hits.
InterProi IPR003018. GAF.
IPR029016. GAF_dom_like.
IPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view ]
Pfami PF01590. GAF. 2 hits.
PF00233. PDEase_I. 1 hit.
[Graphical view ]
PRINTSi PR00387. PDIESTERASE1.
SMARTi SM00065. GAF. 2 hits.
SM00471. HDc. 1 hit.
[Graphical view ]
SUPFAMi SSF55781. SSF55781. 2 hits.
PROSITEi PS00126. PDEASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and characterization of cDNAs encoding PDE5A, a human cGMP-binding, cGMP-specific 3',5'-cyclic nucleotide phosphodiesterase."
    Loughney K., Hill T.R., Florio V.A., Uher L., Rosman G.J., Wolda S.L., Jones B.A., Howard M.L., McAllister-Lucas L.M., Sonnenburg W.K., Francis S.H., Corbin J.D., Beavo J.A., Ferguson K.
    Gene 216:139-147(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS PDE5A1 AND PDE5A2), VARIANT VAL-93.
  2. "Expression, structure and chromosomal localization of the human cGMP-binding cGMP-specific phosphodiesterase PDE5A gene."
    Yanaka N., Kotera J., Ohtsuka A., Akatsuka H., Imai Y., Michibata H., Fujishige K., Kawai E., Takebayashi S., Okumura K., Omori K.
    Eur. J. Biochem. 255:391-399(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM PDE5A1), VARIANT VAL-93.
    Tissue: Lung and Placenta.
  3. "Molecular cloning and expression of human cGMP-binding cGMP-specific phosphodiesterase."
    Stacey P., Rulten S., Dapling A., Phillips S.C.
    Biochem. Biophys. Res. Commun. 247:249-254(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM PDE5A1), VARIANT VAL-93.
    Tissue: Prostate and Skeletal muscle.
  4. "Molecular cloning and characterization of human cGMP-specific phosphodiesterase 5A2 cDNA."
    Kotera J., Imai Y., Omori K.
    Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM PDE5A2), VARIANT VAL-93.
    Tissue: Lung.
  5. "PDE5A splice variants in T84 cells."
    Sopory S., Visweswariah S.S.
    Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM PDE5A2).
    Tissue: Colon carcinoma.
  6. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM PDE5A1), VARIANT VAL-93.
    Tissue: Thalamus.
  7. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
    Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
    , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
    Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  8. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM PDE5A1).
  9. "Multiple cyclic nucleotide phosphodiesterases in human trabecular meshwork cells."
    Zhou L., Thompson W.J., Potter D.E.
    Invest. Ophthalmol. Vis. Sci. 40:1745-1752(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 157-411.
    Tissue: Trabecular meshwork.
  10. "Regulation of cGMP-specific phosphodiesterase (PDE5) phosphorylation in smooth muscle cells."
    Rybalkin S.D., Rybalkina I.G., Feil R., Hofmann F., Beavo J.A.
    J. Biol. Chem. 277:3310-3317(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION BY PRKG1.
  11. "Structure of the catalytic domain of human phosphodiesterase 5 with bound drug molecules."
    Sung B.-J., Hwang K.Y., Jeon Y.H., Lee J.I., Heo Y.-S., Kim J.H., Moon J., Yoon J.M., Hyun Y.-L., Kim E., Eum S.J., Park S.-Y., Lee J.-O., Lee T.G., Ro S., Cho J.M.
    Nature 425:98-102(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 537-860 IN COMPLEX WITH THE INHIBITORS SILDENAFIL; TADALAFIL AND VARDENAFIL, METAL-BINDING.

Entry informationi

Entry nameiPDE5A_HUMAN
AccessioniPrimary (citable) accession number: O76074
Secondary accession number(s): A0AV69
, A8K2C4, O75026, O75887, Q86UI0, Q86V66, Q9Y6Z6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: January 11, 2011
Last modified: September 3, 2014
This is version 144 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  7. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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