Probable cytosolic iron-sulfur protein assembly protein CIAO1

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O76071 (CIAO1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 119. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Probable cytosolic iron-sulfur protein assembly protein CIAO1

Alternative name(s):
WD repeat-containing protein 39

Gene names

Name:	CIAO1
Synonyms:	WDR39

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	339 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Key component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into extramitochondrial Fe/S proteins. Seems to specifically modulate the transactivation activity of WT1. As part of the mitotic spindle-associated MMXD complex it may play a role in chromosome segregation. Ref.8 Ref.9
Subunit structure	Component of the CIA complex. Component of the MMXD complex, which includes CIAO1, ERCC2, FAM96B, MMS19 and SLC25A5. Interacts with WT1. Interacts with FAM96A. Ref.1 Ref.9 Ref.11 Ref.12 Ref.13
Miscellaneous	'Ciao' means 'bridge' in Chinese. HAMAP-Rule MF_03037
Sequence similarities	Belongs to the WD repeat CIA1 family. Contains 7 WD repeats.

Ontologies

Keywords
Biological process	Chromosome partition
Domain	Repeat WD repeat
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	chromosome segregation Inferred from electronic annotation. Source: UniProtKB-KW iron-sulfur cluster assembly Inferred from genetic interaction Ref.8. Source: UniProtKB positive regulation of cell proliferation Traceable author statement Ref.1. Source: ProtInc regulation of transcription from RNA polymerase II promoter Traceable author statement Ref.1. Source: ProtInc
Cellular_component	CIA complex Inferred from direct assay Ref.13 Ref.12. Source: UniProtKB MMXD complex Inferred from direct assay Ref.9. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 339

339

Probable cytosolic iron-sulfur protein assembly protein CIAO1 HAMAP-Rule MF_03037

PRO_0000051389

Regions

Repeat

14 – 53

WD 1 HAMAP-Rule MF_03037

Repeat

59 – 98

WD 2 HAMAP-Rule MF_03037

Repeat

103 – 142

WD 3 HAMAP-Rule MF_03037

Repeat

148 – 187

WD 4 HAMAP-Rule MF_03037

Repeat

192 – 231

WD 5 HAMAP-Rule MF_03037

Repeat

250 – 289

WD 6 HAMAP-Rule MF_03037

Repeat

301 – 339

WD 7 HAMAP-Rule MF_03037

Experimental info

Sequence conflict

243

C → G in BAD96977. Ref.5

Secondary structure

339

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

O76071 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: 63A8D8257A204FC8
Show »

FASTA

339

37,840

        10         20         30         40         50         60 
MKDSLVLLGR VPAHPDSRCW FLAWNPAGTL LASCGGDRRI RIWGTEGDSW ICKSVLSEGH 

        70         80         90        100        110        120 
QRTVRKVAWS PCGNYLASAS FDATTCIWKK NQDDFECVTT LEGHENEVKS VAWAPSGNLL 

       130        140        150        160        170        180 
ATCSRDKSVW VWEVDEEDEY ECVSVLNSHT QDVKHVVWHP SQELLASASY DDTVKLYREE 

       190        200        210        220        230        240 
EDDWVCCATL EGHESTVWSL AFDPSGQRLA SCSDDRTVRI WRQYLPGNEQ GVACSGSDPS 

       250        260        270        280        290        300 
WKCICTLSGF HSRTIYDIAW CQLTGALATA CGDDAIRVFQ EDPNSDPQQP TFSLTAHLHQ 

       310        320        330 
AHSQDVNCVA WNPKEPGLLA SCSDDGEVAF WKYQRPEGL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Ciao 1 is a novel WD40 protein that interacts with the tumor suppressor protein WT1." Johnstone R.W., Wang J., Tommerup N., Vissing H., Roberts T., Shi Y. J. Biol. Chem. 273:10880-10887(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH WT1.
[2]	"CUL4-DDB1 ubiquitin ligase interacts with multiple WD40-repeat proteins and regulates histone methylation." Higa L.A., Wu M., Ye T., Kobayashi R., Sun H., Zhang H. Nat. Cell Biol. 8:1277-1283(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Genome duplications and other features in 12 Mb of DNA sequence from human chromosome 16p and 16q." Loftus B.J., Kim U.-J., Sneddon V.P., Kalush F., Brandon R., Fuhrmann J., Mason T., Crosby M.L., Barnstead M., Cronin L., Mays A.D., Cao Y., Xu R.X., Kang H.-L., Mitchell S., Eichler E.E., Harris P.C., Venter J.C., Adams M.D. Genomics 60:295-308(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Gastric mucosa.
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Blood and Skin.
[7]	"Structural organization, tissue expression, and chromosomal localization of Ciao 1, a functional modulator of the Wilms' tumor suppressor, WT1." Johnstone R.W., Tommerup N., Hansen C., Vissing H., Shi Y. Immunogenetics 49:900-905(1999) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[8]	"Structure of the yeast WD40 domain protein Cia1, a component acting late in iron-sulfur protein biogenesis." Srinivasan V., Netz D.J.A., Webert H., Mascarenhas J., Pierik A.J., Michel H., Lill R. Structure 15:1246-1257(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[9]	"MMXD, a TFIIH-independent XPD-MMS19 protein complex involved in chromosome segregation." Ito S., Tan L.J., Andoh D., Narita T., Seki M., Hirano Y., Narita K., Kuraoka I., Hiraoka Y., Tanaka K. Mol. Cell 39:632-640(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN MMXD COMPLEX.
[10]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]	"The mammalian DUF59 protein Fam96a forms two distinct types of domain-swapped dimer." Chen K.E., Richards A.A., Ariffin J.K., Ross I.L., Sweet M.J., Kellie S., Kobe B., Martin J.L. Acta Crystallogr. D 68:637-648(2012) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH FAM96A.
[12]	"MMS19 assembles iron-sulfur proteins required for DNA metabolism and genomic integrity." Stehling O., Vashisht A.A., Mascarenhas J., Jonsson Z.O., Sharma T., Netz D.J., Pierik A.J., Wohlschlegel J.A., Lill R. Science 337:195-199(2012) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE CIA COMPLEX.
[13]	"MMS19 links cytoplasmic iron-sulfur cluster assembly to DNA metabolism." Gari K., Leon Ortiz A.M., Borel V., Flynn H., Skehel J.M., Boulton S.J. Science 337:243-245(2012) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE CIA COMPLEX.
[14]	"Structure and function of WD40 domain proteins." Xu C., Min J. Protein Cell 2:202-214(2011) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U63810 mRNA. Translation: AAC24948.1.
EF011618 mRNA. Translation: ABK41108.1.
AC004020 Genomic DNA. Translation: AAC23493.1.
CR456802 mRNA. Translation: CAG33083.1.
AK223257 mRNA. Translation: BAD96977.1.
BC001395 mRNA. Translation: AAH01395.1.
BC032812 mRNA. Translation: AAH32812.1.

IPI

IPI00008791.

RefSeq

NP_004795.1. NM_004804.2.

UniGene

Hs.12109.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3FM0	X-ray	1.70	A	1-339	[»]

ProteinModelPortal

O76071.

ModBase

Search...

Protein-protein interaction databases

IntAct

O76071. 7 interactions.

MINT

MINT-1416717.

STRING

9606.ENSP00000418287.

PTM databases

PhosphoSite

O76071.

Proteomic databases

PaxDb

O76071.

PeptideAtlas

O76071.

PRIDE

O76071.

Protocols and materials databases

DNASU

9391.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000488633; ENSP00000418287; ENSG00000144021.

GeneID

9391.

KEGG

hsa:9391.

UCSC

uc002svs.3. human.

Organism-specific databases

CTD

9391.

GeneCards

GC02P096931.

HGNC

HGNC:14280. CIAO1.

HPA

HPA017882.

MIM

604333. gene.

neXtProt

NX_O76071.

PharmGKB

PA162382269.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG2319.

HOGENOM

HOG000208901.

HOVERGEN

HBG056532.

InParanoid

O76071.

OMA

SWKCVCT.

OrthoDB

EOG4MCX0N.

PhylomeDB

O76071.

Gene expression databases

Bgee

O76071.

CleanEx

HS_CIAO1.

Genevestigator

O76071.

GermOnline

ENSG00000144021. Homo sapiens.

Family and domain databases

Gene3D

2.130.10.10. 1 hit.

HAMAP

MF_03037. ciao1.

InterPro

IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]

Pfam

PF00400. WD40. 7 hits.
[Graphical view]

SMART

SM00320. WD40. 7 hits.
[Graphical view]

SUPFAM

SSF50978. WD40_like. 1 hit.

PROSITE

PS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

CIAO1. human.

EvolutionaryTrace

O76071.

GenomeRNAi

9391.

NextBio

35187.

SOURCE

Search...

Entry information

Entry name

CIAO1_HUMAN

Accession

Primary (citable) accession number: O76071
Secondary accession number(s): A0MNN9, Q53FM5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	November 1, 1998
Last modified:	May 1, 2013
This is version 119 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

Human chromosome 2

Human chromosome 2: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents