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O76071 (CIAO1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 129. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable cytosolic iron-sulfur protein assembly protein CIAO1
Alternative name(s):
WD repeat-containing protein 39
Gene names
Name:CIAO1
Synonyms:WDR39
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length339 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Key component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into extramitochondrial Fe/S proteins. Seems to specifically modulate the transactivation activity of WT1. As part of the mitotic spindle-associated MMXD complex it may play a role in chromosome segregation. Ref.8 Ref.9

Subunit structure

Component of the CIA complex. Component of the MMXD complex, which includes CIAO1, ERCC2, FAM96B, MMS19 and SLC25A5. Interacts with WT1. Interacts with FAM96A. Ref.1 Ref.9 Ref.11 Ref.13 Ref.14

Miscellaneous

'Ciao' means 'bridge' in Chinese.

Sequence similarities

Belongs to the WD repeat CIA1 family.

Contains 7 WD repeats.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 339339Probable cytosolic iron-sulfur protein assembly protein CIAO1 HAMAP-Rule MF_03037
PRO_0000051389

Regions

Repeat14 – 5340WD 1 HAMAP-Rule MF_03037
Repeat59 – 9840WD 2 HAMAP-Rule MF_03037
Repeat103 – 14240WD 3 HAMAP-Rule MF_03037
Repeat148 – 18740WD 4 HAMAP-Rule MF_03037
Repeat192 – 23140WD 5 HAMAP-Rule MF_03037
Repeat250 – 28940WD 6 HAMAP-Rule MF_03037
Repeat301 – 33939WD 7 HAMAP-Rule MF_03037

Experimental info

Sequence conflict2431C → G in BAD96977. Ref.5

Secondary structure

................................................................. 339
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
O76071 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: 63A8D8257A204FC8

FASTA33937,840
        10         20         30         40         50         60 
MKDSLVLLGR VPAHPDSRCW FLAWNPAGTL LASCGGDRRI RIWGTEGDSW ICKSVLSEGH 

        70         80         90        100        110        120 
QRTVRKVAWS PCGNYLASAS FDATTCIWKK NQDDFECVTT LEGHENEVKS VAWAPSGNLL 

       130        140        150        160        170        180 
ATCSRDKSVW VWEVDEEDEY ECVSVLNSHT QDVKHVVWHP SQELLASASY DDTVKLYREE 

       190        200        210        220        230        240 
EDDWVCCATL EGHESTVWSL AFDPSGQRLA SCSDDRTVRI WRQYLPGNEQ GVACSGSDPS 

       250        260        270        280        290        300 
WKCICTLSGF HSRTIYDIAW CQLTGALATA CGDDAIRVFQ EDPNSDPQQP TFSLTAHLHQ 

       310        320        330 
AHSQDVNCVA WNPKEPGLLA SCSDDGEVAF WKYQRPEGL 

« Hide

References

« Hide 'large scale' references
[1]"Ciao 1 is a novel WD40 protein that interacts with the tumor suppressor protein WT1."
Johnstone R.W., Wang J., Tommerup N., Vissing H., Roberts T., Shi Y.
J. Biol. Chem. 273:10880-10887(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH WT1.
[2]"CUL4-DDB1 ubiquitin ligase interacts with multiple WD40-repeat proteins and regulates histone methylation."
Higa L.A., Wu M., Ye T., Kobayashi R., Sun H., Zhang H.
Nat. Cell Biol. 8:1277-1283(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Genome duplications and other features in 12 Mb of DNA sequence from human chromosome 16p and 16q."
Loftus B.J., Kim U.-J., Sneddon V.P., Kalush F., Brandon R., Fuhrmann J., Mason T., Crosby M.L., Barnstead M., Cronin L., Mays A.D., Cao Y., Xu R.X., Kang H.-L., Mitchell S., Eichler E.E., Harris P.C., Venter J.C., Adams M.D.
Genomics 60:295-308(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Gastric mucosa.
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Blood and Skin.
[7]"Structural organization, tissue expression, and chromosomal localization of Ciao 1, a functional modulator of the Wilms' tumor suppressor, WT1."
Johnstone R.W., Tommerup N., Hansen C., Vissing H., Shi Y.
Immunogenetics 49:900-905(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[8]"Structure of the yeast WD40 domain protein Cia1, a component acting late in iron-sulfur protein biogenesis."
Srinivasan V., Netz D.J.A., Webert H., Mascarenhas J., Pierik A.J., Michel H., Lill R.
Structure 15:1246-1257(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[9]"MMXD, a TFIIH-independent XPD-MMS19 protein complex involved in chromosome segregation."
Ito S., Tan L.J., Andoh D., Narita T., Seki M., Hirano Y., Narita K., Kuraoka I., Hiraoka Y., Tanaka K.
Mol. Cell 39:632-640(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, IDENTIFICATION IN MMXD COMPLEX.
[10]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"The mammalian DUF59 protein Fam96a forms two distinct types of domain-swapped dimer."
Chen K.E., Richards A.A., Ariffin J.K., Ross I.L., Sweet M.J., Kellie S., Kobe B., Martin J.L.
Acta Crystallogr. D 68:637-648(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH FAM96A.
[12]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[13]"MMS19 assembles iron-sulfur proteins required for DNA metabolism and genomic integrity."
Stehling O., Vashisht A.A., Mascarenhas J., Jonsson Z.O., Sharma T., Netz D.J., Pierik A.J., Wohlschlegel J.A., Lill R.
Science 337:195-199(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE CIA COMPLEX.
[14]"MMS19 links cytoplasmic iron-sulfur cluster assembly to DNA metabolism."
Gari K., Leon Ortiz A.M., Borel V., Flynn H., Skehel J.M., Boulton S.J.
Science 337:243-245(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE CIA COMPLEX.
[15]"Structure and function of WD40 domain proteins."
Xu C., Min J.
Protein Cell 2:202-214(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U63810 mRNA. Translation: AAC24948.1.
EF011618 mRNA. Translation: ABK41108.1.
AC004020 Genomic DNA. Translation: AAC23493.1.
CR456802 mRNA. Translation: CAG33083.1.
AK223257 mRNA. Translation: BAD96977.1.
BC001395 mRNA. Translation: AAH01395.1.
BC032812 mRNA. Translation: AAH32812.1.
CCDSCCDS2019.1.
RefSeqNP_004795.1. NM_004804.2.
UniGeneHs.12109.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3FM0X-ray1.70A1-339[»]
ProteinModelPortalO76071.
SMRO76071. Positions 4-335.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid114791. 19 interactions.
IntActO76071. 9 interactions.
MINTMINT-1416717.
STRING9606.ENSP00000418287.

PTM databases

PhosphoSiteO76071.

Proteomic databases

MaxQBO76071.
PaxDbO76071.
PeptideAtlasO76071.
PRIDEO76071.

Protocols and materials databases

DNASU9391.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000488633; ENSP00000418287; ENSG00000144021.
GeneID9391.
KEGGhsa:9391.
UCSCuc002svs.3. human.

Organism-specific databases

CTD9391.
GeneCardsGC02P096931.
HGNCHGNC:14280. CIAO1.
HPAHPA017882.
MIM604333. gene.
neXtProtNX_O76071.
PharmGKBPA162382269.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG2319.
HOGENOMHOG000208901.
HOVERGENHBG056532.
InParanoidO76071.
OMAVWKCVCT.
OrthoDBEOG7XH6Q1.
PhylomeDBO76071.
TreeFamTF318181.

Enzyme and pathway databases

ReactomeREACT_111217. Metabolism.
SignaLinkO76071.

Gene expression databases

BgeeO76071.
CleanExHS_CIAO1.
GenevestigatorO76071.

Family and domain databases

Gene3D2.130.10.10. 1 hit.
HAMAPMF_03037. ciao1.
InterProIPR028608. CIAO1/Cia1.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamPF00400. WD40. 7 hits.
[Graphical view]
SMARTSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMSSF50978. SSF50978. 1 hit.
PROSITEPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSCIAO1. human.
EvolutionaryTraceO76071.
GeneWikiCIAO1.
GenomeRNAi9391.
NextBio35187.
PROO76071.
SOURCESearch...

Entry information

Entry nameCIAO1_HUMAN
AccessionPrimary (citable) accession number: O76071
Secondary accession number(s): A0MNN9, Q53FM5
Entry history
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: November 1, 1998
Last modified: July 9, 2014
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 2

Human chromosome 2: entries, gene names and cross-references to MIM