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O76071

- CIAO1_HUMAN

UniProt

O76071 - CIAO1_HUMAN

Protein

Probable cytosolic iron-sulfur protein assembly protein CIAO1

Gene

CIAO1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 131 (01 Oct 2014)
      Sequence version 1 (01 Nov 1998)
      Previous versions | rss
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    Functioni

    Key component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into extramitochondrial Fe/S proteins. Seems to specifically modulate the transactivation activity of WT1. As part of the mitotic spindle-associated MMXD complex it may play a role in chromosome segregation.2 PublicationsUniRule annotation

    GO - Biological processi

    1. chromosome segregation Source: UniProtKB-KW
    2. iron-sulfur cluster assembly Source: UniProtKB
    3. positive regulation of cell proliferation Source: ProtInc
    4. regulation of transcription from RNA polymerase II promoter Source: ProtInc
    5. small molecule metabolic process Source: Reactome

    Keywords - Biological processi

    Chromosome partition

    Enzyme and pathway databases

    ReactomeiREACT_160176. Cytosolic iron-sulfur cluster assembly.
    SignaLinkiO76071.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable cytosolic iron-sulfur protein assembly protein CIAO1UniRule annotation
    Alternative name(s):
    WD repeat-containing protein 39UniRule annotation
    Gene namesi
    Name:CIAO1UniRule annotation
    Synonyms:WDR39
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 2

    Organism-specific databases

    HGNCiHGNC:14280. CIAO1.

    Subcellular locationi

    GO - Cellular componenti

    1. CIA complex Source: UniProtKB
    2. MMXD complex Source: UniProtKB

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA162382269.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 339339Probable cytosolic iron-sulfur protein assembly protein CIAO1PRO_0000051389Add
    BLAST

    Proteomic databases

    MaxQBiO76071.
    PaxDbiO76071.
    PeptideAtlasiO76071.
    PRIDEiO76071.

    PTM databases

    PhosphoSiteiO76071.

    Expressioni

    Gene expression databases

    BgeeiO76071.
    CleanExiHS_CIAO1.
    GenevestigatoriO76071.

    Organism-specific databases

    HPAiHPA017882.

    Interactioni

    Subunit structurei

    Component of the CIA complex. Component of the MMXD complex, which includes CIAO1, ERCC2, FAM96B, MMS19 and SLC25A5. Interacts with WT1. Interacts with FAM96A.5 PublicationsUniRule annotation

    Protein-protein interaction databases

    BioGridi114791. 20 interactions.
    IntActiO76071. 9 interactions.
    MINTiMINT-1416717.
    STRINGi9606.ENSP00000418287.

    Structurei

    Secondary structure

    1
    339
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi5 – 117
    Beta strandi19 – 246
    Beta strandi31 – 355
    Beta strandi40 – 467
    Beta strandi49 – 568
    Beta strandi64 – 696
    Beta strandi73 – 808
    Beta strandi85 – 906
    Beta strandi95 – 1017
    Beta strandi108 – 1136
    Beta strandi117 – 1248
    Beta strandi129 – 1346
    Beta strandi140 – 1467
    Beta strandi153 – 1586
    Beta strandi160 – 1634
    Beta strandi165 – 1695
    Beta strandi174 – 1807
    Beta strandi183 – 1908
    Beta strandi197 – 2026
    Beta strandi206 – 2138
    Beta strandi218 – 2247
    Beta strandi240 – 2478
    Beta strandi255 – 2606
    Turni262 – 2643
    Beta strandi267 – 2715
    Beta strandi276 – 2816
    Beta strandi292 – 2987
    Beta strandi301 – 3044
    Beta strandi306 – 3116
    Beta strandi313 – 3153
    Beta strandi318 – 3236
    Beta strandi328 – 3336

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3FM0X-ray1.70A1-339[»]
    ProteinModelPortaliO76071.
    SMRiO76071. Positions 4-335.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO76071.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati14 – 5340WD 1Add
    BLAST
    Repeati59 – 9840WD 2Add
    BLAST
    Repeati103 – 14240WD 3Add
    BLAST
    Repeati148 – 18740WD 4Add
    BLAST
    Repeati192 – 23140WD 5Add
    BLAST
    Repeati250 – 28940WD 6Add
    BLAST
    Repeati301 – 33939WD 7Add
    BLAST

    Sequence similaritiesi

    Belongs to the WD repeat CIA1 family.UniRule annotation
    Contains 7 WD repeats.UniRule annotation

    Keywords - Domaini

    Repeat, WD repeat

    Phylogenomic databases

    eggNOGiCOG2319.
    HOGENOMiHOG000208901.
    HOVERGENiHBG056532.
    InParanoidiO76071.
    OMAiVWKCVCT.
    OrthoDBiEOG7XH6Q1.
    PhylomeDBiO76071.
    TreeFamiTF318181.

    Family and domain databases

    Gene3Di2.130.10.10. 1 hit.
    HAMAPiMF_03037. ciao1.
    InterProiIPR028608. CIAO1/Cia1.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    [Graphical view]
    PfamiPF00400. WD40. 7 hits.
    [Graphical view]
    SMARTiSM00320. WD40. 7 hits.
    [Graphical view]
    SUPFAMiSSF50978. SSF50978. 1 hit.
    PROSITEiPS00678. WD_REPEATS_1. 1 hit.
    PS50082. WD_REPEATS_2. 6 hits.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O76071-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKDSLVLLGR VPAHPDSRCW FLAWNPAGTL LASCGGDRRI RIWGTEGDSW    50
    ICKSVLSEGH QRTVRKVAWS PCGNYLASAS FDATTCIWKK NQDDFECVTT 100
    LEGHENEVKS VAWAPSGNLL ATCSRDKSVW VWEVDEEDEY ECVSVLNSHT 150
    QDVKHVVWHP SQELLASASY DDTVKLYREE EDDWVCCATL EGHESTVWSL 200
    AFDPSGQRLA SCSDDRTVRI WRQYLPGNEQ GVACSGSDPS WKCICTLSGF 250
    HSRTIYDIAW CQLTGALATA CGDDAIRVFQ EDPNSDPQQP TFSLTAHLHQ 300
    AHSQDVNCVA WNPKEPGLLA SCSDDGEVAF WKYQRPEGL 339
    Length:339
    Mass (Da):37,840
    Last modified:November 1, 1998 - v1
    Checksum:i63A8D8257A204FC8
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti243 – 2431C → G in BAD96977. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U63810 mRNA. Translation: AAC24948.1.
    EF011618 mRNA. Translation: ABK41108.1.
    AC004020 Genomic DNA. Translation: AAC23493.1.
    CR456802 mRNA. Translation: CAG33083.1.
    AK223257 mRNA. Translation: BAD96977.1.
    BC001395 mRNA. Translation: AAH01395.1.
    BC032812 mRNA. Translation: AAH32812.1.
    CCDSiCCDS2019.1.
    RefSeqiNP_004795.1. NM_004804.2.
    UniGeneiHs.12109.

    Genome annotation databases

    EnsembliENST00000488633; ENSP00000418287; ENSG00000144021.
    GeneIDi9391.
    KEGGihsa:9391.
    UCSCiuc002svs.3. human.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U63810 mRNA. Translation: AAC24948.1 .
    EF011618 mRNA. Translation: ABK41108.1 .
    AC004020 Genomic DNA. Translation: AAC23493.1 .
    CR456802 mRNA. Translation: CAG33083.1 .
    AK223257 mRNA. Translation: BAD96977.1 .
    BC001395 mRNA. Translation: AAH01395.1 .
    BC032812 mRNA. Translation: AAH32812.1 .
    CCDSi CCDS2019.1.
    RefSeqi NP_004795.1. NM_004804.2.
    UniGenei Hs.12109.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3FM0 X-ray 1.70 A 1-339 [» ]
    ProteinModelPortali O76071.
    SMRi O76071. Positions 4-335.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 114791. 20 interactions.
    IntActi O76071. 9 interactions.
    MINTi MINT-1416717.
    STRINGi 9606.ENSP00000418287.

    PTM databases

    PhosphoSitei O76071.

    Proteomic databases

    MaxQBi O76071.
    PaxDbi O76071.
    PeptideAtlasi O76071.
    PRIDEi O76071.

    Protocols and materials databases

    DNASUi 9391.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000488633 ; ENSP00000418287 ; ENSG00000144021 .
    GeneIDi 9391.
    KEGGi hsa:9391.
    UCSCi uc002svs.3. human.

    Organism-specific databases

    CTDi 9391.
    GeneCardsi GC02P096931.
    HGNCi HGNC:14280. CIAO1.
    HPAi HPA017882.
    MIMi 604333. gene.
    neXtProti NX_O76071.
    PharmGKBi PA162382269.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG2319.
    HOGENOMi HOG000208901.
    HOVERGENi HBG056532.
    InParanoidi O76071.
    OMAi VWKCVCT.
    OrthoDBi EOG7XH6Q1.
    PhylomeDBi O76071.
    TreeFami TF318181.

    Enzyme and pathway databases

    Reactomei REACT_160176. Cytosolic iron-sulfur cluster assembly.
    SignaLinki O76071.

    Miscellaneous databases

    ChiTaRSi CIAO1. human.
    EvolutionaryTracei O76071.
    GeneWikii CIAO1.
    GenomeRNAii 9391.
    NextBioi 35187.
    PROi O76071.
    SOURCEi Search...

    Gene expression databases

    Bgeei O76071.
    CleanExi HS_CIAO1.
    Genevestigatori O76071.

    Family and domain databases

    Gene3Di 2.130.10.10. 1 hit.
    HAMAPi MF_03037. ciao1.
    InterProi IPR028608. CIAO1/Cia1.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    [Graphical view ]
    Pfami PF00400. WD40. 7 hits.
    [Graphical view ]
    SMARTi SM00320. WD40. 7 hits.
    [Graphical view ]
    SUPFAMi SSF50978. SSF50978. 1 hit.
    PROSITEi PS00678. WD_REPEATS_1. 1 hit.
    PS50082. WD_REPEATS_2. 6 hits.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Ciao 1 is a novel WD40 protein that interacts with the tumor suppressor protein WT1."
      Johnstone R.W., Wang J., Tommerup N., Vissing H., Roberts T., Shi Y.
      J. Biol. Chem. 273:10880-10887(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH WT1.
    2. "CUL4-DDB1 ubiquitin ligase interacts with multiple WD40-repeat proteins and regulates histone methylation."
      Higa L.A., Wu M., Ye T., Kobayashi R., Sun H., Zhang H.
      Nat. Cell Biol. 8:1277-1283(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
      Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
      Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    5. Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
      Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Gastric mucosa.
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Blood and Skin.
    7. "Structural organization, tissue expression, and chromosomal localization of Ciao 1, a functional modulator of the Wilms' tumor suppressor, WT1."
      Johnstone R.W., Tommerup N., Hansen C., Vissing H., Shi Y.
      Immunogenetics 49:900-905(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    8. "Structure of the yeast WD40 domain protein Cia1, a component acting late in iron-sulfur protein biogenesis."
      Srinivasan V., Netz D.J.A., Webert H., Mascarenhas J., Pierik A.J., Michel H., Lill R.
      Structure 15:1246-1257(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    9. "MMXD, a TFIIH-independent XPD-MMS19 protein complex involved in chromosome segregation."
      Ito S., Tan L.J., Andoh D., Narita T., Seki M., Hirano Y., Narita K., Kuraoka I., Hiraoka Y., Tanaka K.
      Mol. Cell 39:632-640(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, IDENTIFICATION IN MMXD COMPLEX.
    10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "The mammalian DUF59 protein Fam96a forms two distinct types of domain-swapped dimer."
      Chen K.E., Richards A.A., Ariffin J.K., Ross I.L., Sweet M.J., Kellie S., Kobe B., Martin J.L.
      Acta Crystallogr. D 68:637-648(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH FAM96A.
    12. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    13. "MMS19 assembles iron-sulfur proteins required for DNA metabolism and genomic integrity."
      Stehling O., Vashisht A.A., Mascarenhas J., Jonsson Z.O., Sharma T., Netz D.J., Pierik A.J., Wohlschlegel J.A., Lill R.
      Science 337:195-199(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE CIA COMPLEX.
    14. "MMS19 links cytoplasmic iron-sulfur cluster assembly to DNA metabolism."
      Gari K., Leon Ortiz A.M., Borel V., Flynn H., Skehel J.M., Boulton S.J.
      Science 337:243-245(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE CIA COMPLEX.
    15. "Structure and function of WD40 domain proteins."
      Xu C., Min J.
      Protein Cell 2:202-214(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS).

    Entry informationi

    Entry nameiCIAO1_HUMAN
    AccessioniPrimary (citable) accession number: O76071
    Secondary accession number(s): A0MNN9, Q53FM5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 11, 2001
    Last sequence update: November 1, 1998
    Last modified: October 1, 2014
    This is version 131 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Miscellaneous

    'Ciao' means 'bridge' in Chinese.

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 2
      Human chromosome 2: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3