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Protein

Probable cytosolic iron-sulfur protein assembly protein CIAO1

Gene

CIAO1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Key component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into extramitochondrial Fe/S proteins. Seems to specifically modulate the transactivation activity of WT1. As part of the mitotic spindle-associated MMXD complex it may play a role in chromosome segregation.UniRule annotation2 Publications

GO - Biological processi

  • chromosome segregation Source: UniProtKB-KW
  • iron-sulfur cluster assembly Source: UniProtKB
  • positive regulation of cell proliferation Source: ProtInc
  • regulation of transcription from RNA polymerase II promoter Source: ProtInc
  • small molecule metabolic process Source: Reactome
Complete GO annotation...

Keywords - Biological processi

Chromosome partition

Enzyme and pathway databases

ReactomeiREACT_160176. Cytosolic iron-sulfur cluster assembly.
SignaLinkiO76071.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable cytosolic iron-sulfur protein assembly protein CIAO1UniRule annotation
Alternative name(s):
WD repeat-containing protein 39UniRule annotation
Gene namesi
Name:CIAO1UniRule annotation
Synonyms:WDR39
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:14280. CIAO1.

Subcellular locationi

GO - Cellular componenti

  • CIA complex Source: UniProtKB
  • MMXD complex Source: UniProtKB
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA162382269.

Polymorphism and mutation databases

BioMutaiCIAO1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 339339Probable cytosolic iron-sulfur protein assembly protein CIAO1PRO_0000051389Add
BLAST

Proteomic databases

MaxQBiO76071.
PaxDbiO76071.
PeptideAtlasiO76071.
PRIDEiO76071.

PTM databases

PhosphoSiteiO76071.

Expressioni

Gene expression databases

BgeeiO76071.
CleanExiHS_CIAO1.
GenevisibleiO76071. HS.

Organism-specific databases

HPAiHPA017882.

Interactioni

Subunit structurei

Component of the CIA complex. Component of the MMXD complex, which includes CIAO1, ERCC2, FAM96B, MMS19 and SLC25A5. Interacts with WT1. Interacts with FAM96A.UniRule annotation5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
FAM96AQ9H5X16EBI-725145,EBI-752069
FAM96BQ9Y3D07EBI-725145,EBI-744045
MEOX1P502213EBI-725145,EBI-2864512
MMS19Q96T76-85EBI-725145,EBI-10190644
SF1Q156373EBI-725145,EBI-744603

Protein-protein interaction databases

BioGridi114791. 74 interactions.
IntActiO76071. 11 interactions.
MINTiMINT-1416717.
STRINGi9606.ENSP00000418287.

Structurei

Secondary structure

1
339
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi5 – 117Combined sources
Beta strandi19 – 246Combined sources
Beta strandi31 – 355Combined sources
Beta strandi40 – 467Combined sources
Beta strandi49 – 568Combined sources
Beta strandi64 – 696Combined sources
Beta strandi73 – 808Combined sources
Beta strandi85 – 906Combined sources
Beta strandi95 – 1017Combined sources
Beta strandi108 – 1136Combined sources
Beta strandi117 – 1248Combined sources
Beta strandi129 – 1346Combined sources
Beta strandi140 – 1467Combined sources
Beta strandi153 – 1586Combined sources
Beta strandi160 – 1634Combined sources
Beta strandi165 – 1695Combined sources
Beta strandi174 – 1807Combined sources
Beta strandi183 – 1908Combined sources
Beta strandi197 – 2026Combined sources
Beta strandi206 – 2138Combined sources
Beta strandi218 – 2247Combined sources
Beta strandi240 – 2478Combined sources
Beta strandi255 – 2606Combined sources
Turni262 – 2643Combined sources
Beta strandi267 – 2715Combined sources
Beta strandi276 – 2816Combined sources
Beta strandi292 – 2987Combined sources
Beta strandi301 – 3044Combined sources
Beta strandi306 – 3116Combined sources
Beta strandi313 – 3153Combined sources
Beta strandi318 – 3236Combined sources
Beta strandi328 – 3336Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3FM0X-ray1.70A1-339[»]
ProteinModelPortaliO76071.
SMRiO76071. Positions 4-335.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO76071.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati14 – 5340WD 1Add
BLAST
Repeati59 – 9840WD 2Add
BLAST
Repeati103 – 14240WD 3Add
BLAST
Repeati148 – 18740WD 4Add
BLAST
Repeati192 – 23140WD 5Add
BLAST
Repeati250 – 28940WD 6Add
BLAST
Repeati301 – 33939WD 7Add
BLAST

Sequence similaritiesi

Belongs to the WD repeat CIA1 family.UniRule annotation
Contains 7 WD repeats.UniRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiCOG2319.
GeneTreeiENSGT00800000124097.
HOGENOMiHOG000208901.
HOVERGENiHBG056532.
InParanoidiO76071.
OMAiVWKCVCT.
OrthoDBiEOG7XH6Q1.
PhylomeDBiO76071.
TreeFamiTF318181.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
HAMAPiMF_03037. ciao1.
InterProiIPR028608. CIAO1/Cia1.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 7 hits.
[Graphical view]
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O76071-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKDSLVLLGR VPAHPDSRCW FLAWNPAGTL LASCGGDRRI RIWGTEGDSW
60 70 80 90 100
ICKSVLSEGH QRTVRKVAWS PCGNYLASAS FDATTCIWKK NQDDFECVTT
110 120 130 140 150
LEGHENEVKS VAWAPSGNLL ATCSRDKSVW VWEVDEEDEY ECVSVLNSHT
160 170 180 190 200
QDVKHVVWHP SQELLASASY DDTVKLYREE EDDWVCCATL EGHESTVWSL
210 220 230 240 250
AFDPSGQRLA SCSDDRTVRI WRQYLPGNEQ GVACSGSDPS WKCICTLSGF
260 270 280 290 300
HSRTIYDIAW CQLTGALATA CGDDAIRVFQ EDPNSDPQQP TFSLTAHLHQ
310 320 330
AHSQDVNCVA WNPKEPGLLA SCSDDGEVAF WKYQRPEGL
Length:339
Mass (Da):37,840
Last modified:November 1, 1998 - v1
Checksum:i63A8D8257A204FC8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti243 – 2431C → G in BAD96977 (Ref. 5) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U63810 mRNA. Translation: AAC24948.1.
EF011618 mRNA. Translation: ABK41108.1.
AC004020 Genomic DNA. Translation: AAC23493.1.
CR456802 mRNA. Translation: CAG33083.1.
AK223257 mRNA. Translation: BAD96977.1.
BC001395 mRNA. Translation: AAH01395.1.
BC032812 mRNA. Translation: AAH32812.1.
CCDSiCCDS2019.1.
RefSeqiNP_004795.1. NM_004804.2.
UniGeneiHs.12109.

Genome annotation databases

EnsembliENST00000488633; ENSP00000418287; ENSG00000144021.
GeneIDi9391.
KEGGihsa:9391.
UCSCiuc002svs.3. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U63810 mRNA. Translation: AAC24948.1.
EF011618 mRNA. Translation: ABK41108.1.
AC004020 Genomic DNA. Translation: AAC23493.1.
CR456802 mRNA. Translation: CAG33083.1.
AK223257 mRNA. Translation: BAD96977.1.
BC001395 mRNA. Translation: AAH01395.1.
BC032812 mRNA. Translation: AAH32812.1.
CCDSiCCDS2019.1.
RefSeqiNP_004795.1. NM_004804.2.
UniGeneiHs.12109.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3FM0X-ray1.70A1-339[»]
ProteinModelPortaliO76071.
SMRiO76071. Positions 4-335.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114791. 74 interactions.
IntActiO76071. 11 interactions.
MINTiMINT-1416717.
STRINGi9606.ENSP00000418287.

PTM databases

PhosphoSiteiO76071.

Polymorphism and mutation databases

BioMutaiCIAO1.

Proteomic databases

MaxQBiO76071.
PaxDbiO76071.
PeptideAtlasiO76071.
PRIDEiO76071.

Protocols and materials databases

DNASUi9391.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000488633; ENSP00000418287; ENSG00000144021.
GeneIDi9391.
KEGGihsa:9391.
UCSCiuc002svs.3. human.

Organism-specific databases

CTDi9391.
GeneCardsiGC02P096931.
HGNCiHGNC:14280. CIAO1.
HPAiHPA017882.
MIMi604333. gene.
neXtProtiNX_O76071.
PharmGKBiPA162382269.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG2319.
GeneTreeiENSGT00800000124097.
HOGENOMiHOG000208901.
HOVERGENiHBG056532.
InParanoidiO76071.
OMAiVWKCVCT.
OrthoDBiEOG7XH6Q1.
PhylomeDBiO76071.
TreeFamiTF318181.

Enzyme and pathway databases

ReactomeiREACT_160176. Cytosolic iron-sulfur cluster assembly.
SignaLinkiO76071.

Miscellaneous databases

ChiTaRSiCIAO1. human.
EvolutionaryTraceiO76071.
GeneWikiiCIAO1.
GenomeRNAii9391.
NextBioi35187.
PROiO76071.
SOURCEiSearch...

Gene expression databases

BgeeiO76071.
CleanExiHS_CIAO1.
GenevisibleiO76071. HS.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
HAMAPiMF_03037. ciao1.
InterProiIPR028608. CIAO1/Cia1.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 7 hits.
[Graphical view]
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Ciao 1 is a novel WD40 protein that interacts with the tumor suppressor protein WT1."
    Johnstone R.W., Wang J., Tommerup N., Vissing H., Roberts T., Shi Y.
    J. Biol. Chem. 273:10880-10887(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH WT1.
  2. "CUL4-DDB1 ubiquitin ligase interacts with multiple WD40-repeat proteins and regulates histone methylation."
    Higa L.A., Wu M., Ye T., Kobayashi R., Sun H., Zhang H.
    Nat. Cell Biol. 8:1277-1283(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
    Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Gastric mucosa.
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Blood and Skin.
  7. "Structural organization, tissue expression, and chromosomal localization of Ciao 1, a functional modulator of the Wilms' tumor suppressor, WT1."
    Johnstone R.W., Tommerup N., Hansen C., Vissing H., Shi Y.
    Immunogenetics 49:900-905(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  8. "Structure of the yeast WD40 domain protein Cia1, a component acting late in iron-sulfur protein biogenesis."
    Srinivasan V., Netz D.J.A., Webert H., Mascarenhas J., Pierik A.J., Michel H., Lill R.
    Structure 15:1246-1257(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "MMXD, a TFIIH-independent XPD-MMS19 protein complex involved in chromosome segregation."
    Ito S., Tan L.J., Andoh D., Narita T., Seki M., Hirano Y., Narita K., Kuraoka I., Hiraoka Y., Tanaka K.
    Mol. Cell 39:632-640(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION IN MMXD COMPLEX.
  10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "The mammalian DUF59 protein Fam96a forms two distinct types of domain-swapped dimer."
    Chen K.E., Richards A.A., Ariffin J.K., Ross I.L., Sweet M.J., Kellie S., Kobe B., Martin J.L.
    Acta Crystallogr. D 68:637-648(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH FAM96A.
  12. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. "MMS19 assembles iron-sulfur proteins required for DNA metabolism and genomic integrity."
    Stehling O., Vashisht A.A., Mascarenhas J., Jonsson Z.O., Sharma T., Netz D.J., Pierik A.J., Wohlschlegel J.A., Lill R.
    Science 337:195-199(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE CIA COMPLEX.
  14. "MMS19 links cytoplasmic iron-sulfur cluster assembly to DNA metabolism."
    Gari K., Leon Ortiz A.M., Borel V., Flynn H., Skehel J.M., Boulton S.J.
    Science 337:243-245(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE CIA COMPLEX.
  15. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  16. "Structure and function of WD40 domain proteins."
    Xu C., Min J.
    Protein Cell 2:202-214(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS).

Entry informationi

Entry nameiCIAO1_HUMAN
AccessioniPrimary (citable) accession number: O76071
Secondary accession number(s): A0MNN9, Q53FM5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: November 1, 1998
Last modified: July 22, 2015
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

'Ciao' means 'bridge' in Chinese.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.