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O76071

- CIAO1_HUMAN

UniProt

O76071 - CIAO1_HUMAN

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Protein
Probable cytosolic iron-sulfur protein assembly protein CIAO1
Gene
CIAO1, WDR39
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Key component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into extramitochondrial Fe/S proteins. Seems to specifically modulate the transactivation activity of WT1. As part of the mitotic spindle-associated MMXD complex it may play a role in chromosome segregation.2 Publications

GO - Biological processi

  1. chromosome segregation Source: UniProtKB-KW
  2. iron-sulfur cluster assembly Source: UniProtKB
  3. positive regulation of cell proliferation Source: ProtInc
  4. regulation of transcription from RNA polymerase II promoter Source: ProtInc
  5. small molecule metabolic process Source: Reactome
Complete GO annotation...

Keywords - Biological processi

Chromosome partition

Enzyme and pathway databases

ReactomeiREACT_160176. Cytosolic iron-sulfur cluster assembly.
SignaLinkiO76071.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable cytosolic iron-sulfur protein assembly protein CIAO1
Alternative name(s):
WD repeat-containing protein 39
Gene namesi
Name:CIAO1
Synonyms:WDR39
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 2

Organism-specific databases

HGNCiHGNC:14280. CIAO1.

Subcellular locationi

GO - Cellular componenti

  1. CIA complex Source: UniProtKB
  2. MMXD complex Source: UniProtKB
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA162382269.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 339339Probable cytosolic iron-sulfur protein assembly protein CIAO1UniRule annotation
PRO_0000051389Add
BLAST

Proteomic databases

MaxQBiO76071.
PaxDbiO76071.
PeptideAtlasiO76071.
PRIDEiO76071.

PTM databases

PhosphoSiteiO76071.

Expressioni

Gene expression databases

BgeeiO76071.
CleanExiHS_CIAO1.
GenevestigatoriO76071.

Organism-specific databases

HPAiHPA017882.

Interactioni

Subunit structurei

Component of the CIA complex. Component of the MMXD complex, which includes CIAO1, ERCC2, FAM96B, MMS19 and SLC25A5. Interacts with WT1. Interacts with FAM96A.5 Publications

Protein-protein interaction databases

BioGridi114791. 20 interactions.
IntActiO76071. 9 interactions.
MINTiMINT-1416717.
STRINGi9606.ENSP00000418287.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi5 – 117
Beta strandi19 – 246
Beta strandi31 – 355
Beta strandi40 – 467
Beta strandi49 – 568
Beta strandi64 – 696
Beta strandi73 – 808
Beta strandi85 – 906
Beta strandi95 – 1017
Beta strandi108 – 1136
Beta strandi117 – 1248
Beta strandi129 – 1346
Beta strandi140 – 1467
Beta strandi153 – 1586
Beta strandi160 – 1634
Beta strandi165 – 1695
Beta strandi174 – 1807
Beta strandi183 – 1908
Beta strandi197 – 2026
Beta strandi206 – 2138
Beta strandi218 – 2247
Beta strandi240 – 2478
Beta strandi255 – 2606
Turni262 – 2643
Beta strandi267 – 2715
Beta strandi276 – 2816
Beta strandi292 – 2987
Beta strandi301 – 3044
Beta strandi306 – 3116
Beta strandi313 – 3153
Beta strandi318 – 3236
Beta strandi328 – 3336

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3FM0X-ray1.70A1-339[»]
ProteinModelPortaliO76071.
SMRiO76071. Positions 4-335.

Miscellaneous databases

EvolutionaryTraceiO76071.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati14 – 5340WD 1UniRule annotation
Add
BLAST
Repeati59 – 9840WD 2UniRule annotation
Add
BLAST
Repeati103 – 14240WD 3UniRule annotation
Add
BLAST
Repeati148 – 18740WD 4UniRule annotation
Add
BLAST
Repeati192 – 23140WD 5UniRule annotation
Add
BLAST
Repeati250 – 28940WD 6UniRule annotation
Add
BLAST
Repeati301 – 33939WD 7UniRule annotation
Add
BLAST

Sequence similaritiesi

Belongs to the WD repeat CIA1 family.
Contains 7 WD repeats.

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiCOG2319.
HOGENOMiHOG000208901.
HOVERGENiHBG056532.
InParanoidiO76071.
OMAiVWKCVCT.
OrthoDBiEOG7XH6Q1.
PhylomeDBiO76071.
TreeFamiTF318181.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
HAMAPiMF_03037. ciao1.
InterProiIPR028608. CIAO1/Cia1.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 7 hits.
[Graphical view]
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O76071-1 [UniParc]FASTAAdd to Basket

« Hide

MKDSLVLLGR VPAHPDSRCW FLAWNPAGTL LASCGGDRRI RIWGTEGDSW    50
ICKSVLSEGH QRTVRKVAWS PCGNYLASAS FDATTCIWKK NQDDFECVTT 100
LEGHENEVKS VAWAPSGNLL ATCSRDKSVW VWEVDEEDEY ECVSVLNSHT 150
QDVKHVVWHP SQELLASASY DDTVKLYREE EDDWVCCATL EGHESTVWSL 200
AFDPSGQRLA SCSDDRTVRI WRQYLPGNEQ GVACSGSDPS WKCICTLSGF 250
HSRTIYDIAW CQLTGALATA CGDDAIRVFQ EDPNSDPQQP TFSLTAHLHQ 300
AHSQDVNCVA WNPKEPGLLA SCSDDGEVAF WKYQRPEGL 339
Length:339
Mass (Da):37,840
Last modified:November 1, 1998 - v1
Checksum:i63A8D8257A204FC8
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti243 – 2431C → G in BAD96977. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U63810 mRNA. Translation: AAC24948.1.
EF011618 mRNA. Translation: ABK41108.1.
AC004020 Genomic DNA. Translation: AAC23493.1.
CR456802 mRNA. Translation: CAG33083.1.
AK223257 mRNA. Translation: BAD96977.1.
BC001395 mRNA. Translation: AAH01395.1.
BC032812 mRNA. Translation: AAH32812.1.
CCDSiCCDS2019.1.
RefSeqiNP_004795.1. NM_004804.2.
UniGeneiHs.12109.

Genome annotation databases

EnsembliENST00000488633; ENSP00000418287; ENSG00000144021.
GeneIDi9391.
KEGGihsa:9391.
UCSCiuc002svs.3. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U63810 mRNA. Translation: AAC24948.1 .
EF011618 mRNA. Translation: ABK41108.1 .
AC004020 Genomic DNA. Translation: AAC23493.1 .
CR456802 mRNA. Translation: CAG33083.1 .
AK223257 mRNA. Translation: BAD96977.1 .
BC001395 mRNA. Translation: AAH01395.1 .
BC032812 mRNA. Translation: AAH32812.1 .
CCDSi CCDS2019.1.
RefSeqi NP_004795.1. NM_004804.2.
UniGenei Hs.12109.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3FM0 X-ray 1.70 A 1-339 [» ]
ProteinModelPortali O76071.
SMRi O76071. Positions 4-335.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 114791. 20 interactions.
IntActi O76071. 9 interactions.
MINTi MINT-1416717.
STRINGi 9606.ENSP00000418287.

PTM databases

PhosphoSitei O76071.

Proteomic databases

MaxQBi O76071.
PaxDbi O76071.
PeptideAtlasi O76071.
PRIDEi O76071.

Protocols and materials databases

DNASUi 9391.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000488633 ; ENSP00000418287 ; ENSG00000144021 .
GeneIDi 9391.
KEGGi hsa:9391.
UCSCi uc002svs.3. human.

Organism-specific databases

CTDi 9391.
GeneCardsi GC02P096931.
HGNCi HGNC:14280. CIAO1.
HPAi HPA017882.
MIMi 604333. gene.
neXtProti NX_O76071.
PharmGKBi PA162382269.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG2319.
HOGENOMi HOG000208901.
HOVERGENi HBG056532.
InParanoidi O76071.
OMAi VWKCVCT.
OrthoDBi EOG7XH6Q1.
PhylomeDBi O76071.
TreeFami TF318181.

Enzyme and pathway databases

Reactomei REACT_160176. Cytosolic iron-sulfur cluster assembly.
SignaLinki O76071.

Miscellaneous databases

ChiTaRSi CIAO1. human.
EvolutionaryTracei O76071.
GeneWikii CIAO1.
GenomeRNAii 9391.
NextBioi 35187.
PROi O76071.
SOURCEi Search...

Gene expression databases

Bgeei O76071.
CleanExi HS_CIAO1.
Genevestigatori O76071.

Family and domain databases

Gene3Di 2.130.10.10. 1 hit.
HAMAPi MF_03037. ciao1.
InterProi IPR028608. CIAO1/Cia1.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view ]
Pfami PF00400. WD40. 7 hits.
[Graphical view ]
SMARTi SM00320. WD40. 7 hits.
[Graphical view ]
SUPFAMi SSF50978. SSF50978. 1 hit.
PROSITEi PS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Ciao 1 is a novel WD40 protein that interacts with the tumor suppressor protein WT1."
    Johnstone R.W., Wang J., Tommerup N., Vissing H., Roberts T., Shi Y.
    J. Biol. Chem. 273:10880-10887(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH WT1.
  2. "CUL4-DDB1 ubiquitin ligase interacts with multiple WD40-repeat proteins and regulates histone methylation."
    Higa L.A., Wu M., Ye T., Kobayashi R., Sun H., Zhang H.
    Nat. Cell Biol. 8:1277-1283(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
    Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Gastric mucosa.
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Blood and Skin.
  7. "Structural organization, tissue expression, and chromosomal localization of Ciao 1, a functional modulator of the Wilms' tumor suppressor, WT1."
    Johnstone R.W., Tommerup N., Hansen C., Vissing H., Shi Y.
    Immunogenetics 49:900-905(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  8. "Structure of the yeast WD40 domain protein Cia1, a component acting late in iron-sulfur protein biogenesis."
    Srinivasan V., Netz D.J.A., Webert H., Mascarenhas J., Pierik A.J., Michel H., Lill R.
    Structure 15:1246-1257(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "MMXD, a TFIIH-independent XPD-MMS19 protein complex involved in chromosome segregation."
    Ito S., Tan L.J., Andoh D., Narita T., Seki M., Hirano Y., Narita K., Kuraoka I., Hiraoka Y., Tanaka K.
    Mol. Cell 39:632-640(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION IN MMXD COMPLEX.
  10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "The mammalian DUF59 protein Fam96a forms two distinct types of domain-swapped dimer."
    Chen K.E., Richards A.A., Ariffin J.K., Ross I.L., Sweet M.J., Kellie S., Kobe B., Martin J.L.
    Acta Crystallogr. D 68:637-648(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH FAM96A.
  12. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. "MMS19 assembles iron-sulfur proteins required for DNA metabolism and genomic integrity."
    Stehling O., Vashisht A.A., Mascarenhas J., Jonsson Z.O., Sharma T., Netz D.J., Pierik A.J., Wohlschlegel J.A., Lill R.
    Science 337:195-199(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE CIA COMPLEX.
  14. "MMS19 links cytoplasmic iron-sulfur cluster assembly to DNA metabolism."
    Gari K., Leon Ortiz A.M., Borel V., Flynn H., Skehel J.M., Boulton S.J.
    Science 337:243-245(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE CIA COMPLEX.
  15. "Structure and function of WD40 domain proteins."
    Xu C., Min J.
    Protein Cell 2:202-214(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS).

Entry informationi

Entry nameiCIAO1_HUMAN
AccessioniPrimary (citable) accession number: O76071
Secondary accession number(s): A0MNN9, Q53FM5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: November 1, 1998
Last modified: September 3, 2014
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

'Ciao' means 'bridge' in Chinese.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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