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Protein

A-kinase anchor protein 3

Gene

AKAP3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May function as a regulator of both motility- and head-associated functions such as capacitation and the acrosome reaction.

GO - Molecular functioni

  • protein kinase A binding Source: ProtInc

GO - Biological processi

  • acrosome reaction Source: ProtInc
  • movement of cell or subcellular component Source: ProtInc
  • protein localization Source: Ensembl
  • regulation of protein kinase A signaling Source: GO_Central
  • single fertilization Source: ProtInc
  • transmembrane receptor protein serine/threonine kinase signaling pathway Source: Ensembl
Complete GO annotation...

Enzyme and pathway databases

BioCyciZFISH:ENSG00000111254-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
A-kinase anchor protein 3
Short name:
AKAP-3
Alternative name(s):
A-kinase anchor protein 110 kDa
Short name:
AKAP 110
Cancer/testis antigen 82
Short name:
CT82
Fibrous sheath protein of 95 kDa
Short name:
FSP95
Fibrousheathin I
Fibrousheathin-1
Protein kinase A-anchoring protein 3
Short name:
PRKA3
Sperm oocyte-binding protein
Gene namesi
Name:AKAP3
Synonyms:AKAP110, SOB1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:373. AKAP3.

Subcellular locationi

GO - Cellular componenti

  • acrosomal vesicle Source: UniProtKB-SubCell
  • cytoplasm Source: GO_Central
  • nucleus Source: UniProtKB
  • sperm fibrous sheath Source: GO_Central
  • sperm principal piece Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi131L → P: Abolishes interaction with ROPN1. 1 Publication1

Organism-specific databases

DisGeNETi10566.
OpenTargetsiENSG00000111254.
PharmGKBiPA24667.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000645261 – 853A-kinase anchor protein 3Add BLAST853

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei205PhosphoserineCombined sources1
Modified residuei208PhosphoserineCombined sources1
Modified residuei403PhosphoserineCombined sources1
Modified residuei404PhosphotyrosineCombined sources1
Modified residuei635PhosphoserineCombined sources1
Modified residuei636PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated on tyrosine residues.

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO75969.
PaxDbiO75969.
PeptideAtlasiO75969.
PRIDEiO75969.

PTM databases

iPTMnetiO75969.
PhosphoSitePlusiO75969.

Expressioni

Tissue specificityi

Testis specific; only expressed in spermatids.

Gene expression databases

BgeeiENSG00000111254.
CleanExiHS_AKAP3.
ExpressionAtlasiO75969. baseline and differential.
GenevisibleiO75969. HS.

Organism-specific databases

HPAiHPA039765.

Interactioni

Subunit structurei

Interacts with ROPN1 AND ROPN1L.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
BRAPQ7Z5692EBI-9033101,EBI-349900
PRKAR2AP138612EBI-9033101,EBI-2556122
ROPN1BQ9BZX42EBI-9033101,EBI-9033148

GO - Molecular functioni

  • protein kinase A binding Source: ProtInc

Protein-protein interaction databases

BioGridi115817. 8 interactors.
IntActiO75969. 4 interactors.
STRINGi9606.ENSP00000228850.

Structurei

3D structure databases

ProteinModelPortaliO75969.
SMRiO75969.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni124 – 137PKA-RII subunit binding domainAdd BLAST14

Domaini

RII-binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.

Sequence similaritiesi

Belongs to the AKAP110 family.Curated

Phylogenomic databases

eggNOGiENOG410IEV9. Eukaryota.
ENOG411183Z. LUCA.
GeneTreeiENSGT00420000029845.
HOGENOMiHOG000220883.
HOVERGENiHBG050478.
InParanoidiO75969.
KOiK16520.
OMAiQYHLAQG.
OrthoDBiEOG091G0209.
PhylomeDBiO75969.
TreeFamiTF105403.

Family and domain databases

InterProiIPR020799. AKAP_110.
IPR018292. AKAP_110_C.
IPR018459. RII_binding_1.
IPR008382. SPHK1-interactor_AKAP_110.
[Graphical view]
PANTHERiPTHR10226. PTHR10226. 1 hit.
PfamiPF05716. AKAP_110. 1 hit.
PF10522. RII_binding_1. 1 hit.
[Graphical view]
SMARTiSM00807. AKAP_110. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O75969-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEKVDWLQS QNGVCKVDVY SPGDNQAQDW KMDTSTDPVR VLSWLRRDLE
60 70 80 90 100
KSTAEFQDVR FKPGESFGGE TSNSGDPHKG FSVDYYNTTT KGTPERLHFE
110 120 130 140 150
MTHKEIPCQG PRAQLGNGSS VDEVSFYANR LTNLVIAMAR KEINEKIDGS
160 170 180 190 200
ENKCVYQSLY MGNEPTPTKS LSKIASELVN ETVSACSRNA APDKAPGSGD
210 220 230 240 250
RVSGSSQSPP NLKYKSTLKI KESTKERQGP DDKPPSKKSF FYKEVFESRN
260 270 280 290 300
GDYAREGGRF FPRERKRFRG QERPDDFTAS VSEGIMTYAN SVVSDMMVSI
310 320 330 340 350
MKTLKIQVKD TTIATILLKK VLLKHAKEVV SDLIDSFLRN LHSVTGTLMT
360 370 380 390 400
DTQFVSAVKR TVFSHGSQKA TDIMDAMLRK LYNVMFAKKV PEHVRKAQDK
410 420 430 440 450
AESYSLISMK GMGDPKNRNV NFAMKSETKL REKMYSEPKS EEETCAKTLG
460 470 480 490 500
EHIIKEGLTL WHKTQQKECK SLGFQHAAFE APNTQRKPAS DISFEYPEDI
510 520 530 540 550
GNLSLPPYPP EKPENFMYDS DSWAEDLIVS ALLLIQYHLA QGGRRDARSF
560 570 580 590 600
VEAAGTTNFP ANEPPVAPDE SCLKSAPIVG DQEQAEKKDL RSVFFNFIRN
610 620 630 640 650
LLSETIFKRD QSPEPKVPEQ PVKEDRKLCE RPLASSPPRL YEDDETPGAL
660 670 680 690 700
SGLTKMAVSQ IDGHMSGQMV EHLMNSVMKL CVIIAKSCDA SLAELGDDKS
710 720 730 740 750
GDASRLTSAF PDSLYECLPA KGTGSAEAVL QNAYQAIHNE MRGTSGQPPE
760 770 780 790 800
GCAAPTVIVS NHNLTDTVQN KQLQAVLQWV AASELNVPIL YFAGDDEGIQ
810 820 830 840 850
EKLLQLSAAA VDKGCSVGEV LQSVLRYEKE RQLNEAVGNV TPLQLLDWLM

VNL
Length:853
Mass (Da):94,751
Last modified:December 15, 2009 - v2
Checksum:iCFDEA26922B5A86E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti272E → D in AAD21218 (PubMed:10334916).Curated1
Sequence conflicti282S → G in AAC35854 (PubMed:10529264).Curated1
Sequence conflicti402E → V in AAC35854 (PubMed:10529264).Curated1
Sequence conflicti467K → N in AAC35854 (PubMed:10529264).Curated1
Sequence conflicti597F → S AA sequence (PubMed:10529264).Curated1
Sequence conflicti700S → L in AAC35854 (PubMed:10529264).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_055488118G → E.4 PublicationsCorresponds to variant rs2072355dbSNPEnsembl.1
Natural variantiVAR_060730464T → S.3 PublicationsCorresponds to variant rs11063266dbSNPEnsembl.1
Natural variantiVAR_055489500I → T.1 PublicationCorresponds to variant rs12366671dbSNPEnsembl.1
Natural variantiVAR_061000525E → K.3 PublicationsCorresponds to variant rs1990312dbSNPEnsembl.1
Natural variantiVAR_055490661I → T.Corresponds to variant rs1990313dbSNPEnsembl.1
Natural variantiVAR_055491700S → F.Corresponds to variant rs2041291dbSNPEnsembl.1
Natural variantiVAR_059112700S → P.Corresponds to variant rs2041290dbSNPEnsembl.1
Natural variantiVAR_055492725S → L.Corresponds to variant rs2072357dbSNPEnsembl.1
Natural variantiVAR_036428831R → C in a colorectal cancer sample; somatic mutation. 1 PublicationCorresponds to variant rs143517596dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U85715 mRNA. Translation: AAD21218.1.
AF087003 mRNA. Translation: AAC35854.1.
AF093408 mRNA. Translation: AAC63371.1.
AK292451 mRNA. Translation: BAF85140.1.
AC005832 Genomic DNA. No translation available.
BC047535 mRNA. Translation: AAH47535.1.
CCDSiCCDS8531.1.
RefSeqiNP_001265238.1. NM_001278309.1.
NP_006413.3. NM_006422.3.
XP_005253721.1. XM_005253664.2.
XP_011519210.1. XM_011520908.1.
XP_011519211.1. XM_011520909.1.
XP_011519212.1. XM_011520910.2.
UniGeneiHs.98397.

Genome annotation databases

EnsembliENST00000228850; ENSP00000228850; ENSG00000111254.
ENST00000545990; ENSP00000440994; ENSG00000111254.
GeneIDi10566.
KEGGihsa:10566.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U85715 mRNA. Translation: AAD21218.1.
AF087003 mRNA. Translation: AAC35854.1.
AF093408 mRNA. Translation: AAC63371.1.
AK292451 mRNA. Translation: BAF85140.1.
AC005832 Genomic DNA. No translation available.
BC047535 mRNA. Translation: AAH47535.1.
CCDSiCCDS8531.1.
RefSeqiNP_001265238.1. NM_001278309.1.
NP_006413.3. NM_006422.3.
XP_005253721.1. XM_005253664.2.
XP_011519210.1. XM_011520908.1.
XP_011519211.1. XM_011520909.1.
XP_011519212.1. XM_011520910.2.
UniGeneiHs.98397.

3D structure databases

ProteinModelPortaliO75969.
SMRiO75969.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115817. 8 interactors.
IntActiO75969. 4 interactors.
STRINGi9606.ENSP00000228850.

PTM databases

iPTMnetiO75969.
PhosphoSitePlusiO75969.

Proteomic databases

EPDiO75969.
PaxDbiO75969.
PeptideAtlasiO75969.
PRIDEiO75969.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000228850; ENSP00000228850; ENSG00000111254.
ENST00000545990; ENSP00000440994; ENSG00000111254.
GeneIDi10566.
KEGGihsa:10566.

Organism-specific databases

CTDi10566.
DisGeNETi10566.
GeneCardsiAKAP3.
H-InvDBHIX0026339.
HGNCiHGNC:373. AKAP3.
HPAiHPA039765.
MIMi604689. gene.
neXtProtiNX_O75969.
OpenTargetsiENSG00000111254.
PharmGKBiPA24667.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IEV9. Eukaryota.
ENOG411183Z. LUCA.
GeneTreeiENSGT00420000029845.
HOGENOMiHOG000220883.
HOVERGENiHBG050478.
InParanoidiO75969.
KOiK16520.
OMAiQYHLAQG.
OrthoDBiEOG091G0209.
PhylomeDBiO75969.
TreeFamiTF105403.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000111254-MONOMER.

Miscellaneous databases

ChiTaRSiAKAP3. human.
GeneWikiiAKAP3.
GenomeRNAii10566.
PROiO75969.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000111254.
CleanExiHS_AKAP3.
ExpressionAtlasiO75969. baseline and differential.
GenevisibleiO75969. HS.

Family and domain databases

InterProiIPR020799. AKAP_110.
IPR018292. AKAP_110_C.
IPR018459. RII_binding_1.
IPR008382. SPHK1-interactor_AKAP_110.
[Graphical view]
PANTHERiPTHR10226. PTHR10226. 1 hit.
PfamiPF05716. AKAP_110. 1 hit.
PF10522. RII_binding_1. 1 hit.
[Graphical view]
SMARTiSM00807. AKAP_110. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAKAP3_HUMAN
AccessioniPrimary (citable) accession number: O75969
Secondary accession number(s): O75945, Q86X01, Q9UM61
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: December 15, 2009
Last modified: November 2, 2016
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.