A-kinase anchor protein 3 - Homo sapiens (Human)

Names and origin

Protein names

Recommended name:
    A-kinase anchor protein 3
Alternative name(s):
    Protein kinase A-anchoring protein 3
      Short name=PRKA3
    A-kinase anchor protein 110 kDa
      Short name=AKAP 110
    Sperm oocyte-binding protein
    Fibrousheathin-1
    Fibrousheathin I
    Fibrous sheath protein of 95 kDa
      Short name=FSP95
    Cancer/testis antigen 82
      Short name=CT82

Gene names

Name:	AKAP3
Synonyms:	AKAP110, SOB1

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length	853 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	May function as a regulator of both motility- and head-associated functions such as capacitation and the acrosome reaction.
Subunit structure	Interacts with ROPN1 AND ROPN1L. Ref.6
Subcellular location	Cytoplasmic vesicle › secretory vesicle › acrosome By similarity. Note: Ribs of the fibrous sheath in the principal piece of the sperm tail. Dorsal margin of the acrosomal segment.
Tissue specificity	Testis specific; only expressed in spermatids.
Domain	RII-binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.
Post-translational modification	Phosphorylated on tyrosine residues. Ref.7
Sequence similarities	Belongs to the AKAP110 family.

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Ontologies

Keywords
Cellular component	Cytoplasmic vesicle
Coding sequence diversity	Polymorphism
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	acrosome reaction Ref.3 Traceable author statement. Source: ProtInc cell motion Ref.3 Traceable author statement. Source: ProtInc
Cellular component	acrosomal vesicle Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein kinase A binding Ref.3 Traceable author statement. Source: ProtInc
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 853

853

A-kinase anchor protein 3

PRO_0000064526

Regions

Region

124 – 137

14

PKA-RII subunit binding domain

Amino acid modifications

Modified residue

205

1

Phosphoserine Ref.7

Modified residue

208

1

Phosphoserine Ref.7

Modified residue

403

1

Phosphoserine Ref.7

Modified residue

404

1

Phosphotyrosine Ref.7

Modified residue

440

1

Phosphoserine Ref.7

Modified residue

612

1

Phosphoserine Ref.7

Modified residue

635

1

Phosphoserine Ref.7

Modified residue

636

1

Phosphoserine Ref.7

Natural variations

Natural variant

118

1

G → E: dbSNP rs2072355. Ref.2 Ref.4 Ref.5

VAR_055488

Natural variant

500

1

I → T: dbSNP rs12366671. Ref.2

VAR_055489

Natural variant

661

1

I → T: dbSNP rs1990313.

VAR_055490

Natural variant

700

1

S → F: dbSNP rs2041291.

VAR_055491

Natural variant

700

1

S → P: dbSNP rs2041290.

VAR_059112

Natural variant

725

1

S → L: dbSNP rs2072357.

VAR_055492

Natural variant

831

1

R → C in a colorectal cancer sample; somatic mutation. Ref.8

VAR_036428

Experimental info

Mutagenesis

131

1

L → P: Abolishes interaction with ROPN1. Ref.6

Sequence conflict

272

1

E → D in AAD21218. Ref.1

Sequence conflict

282

1

S → G in AAC35854. Ref.2

Sequence conflict

467

1

K → N in AAC35854. Ref.2

Sequence conflict

597

1

F → S AA sequence Ref.2

Sequence conflict

700

1

S → L in AAC35854. Ref.2

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Sequences

Sequence

Length

Mass (Da)

Tools

O75969-1 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: 8374FFF1009BEBEC
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FASTA

853

94,736

        10         20         30         40         50         60 
MSEKVDWLQS QNGVCKVDVY SPGDNQAQDW KMDTSTDPVR VLSWLRRDLE KSTAEFQDVR 

        70         80         90        100        110        120 
FKPGESFGGE TSNSGDPHKG FSVDYYNTTT KGTPERLHFE MTHKEIPCQG PRAQLGNGSS 

       130        140        150        160        170        180 
VDEVSFYANR LTNLVIAMAR KEINEKIDGS ENKCVYQSLY MGNEPTPTKS LSKIASELVN 

       190        200        210        220        230        240 
ETVSACSRNA APDKAPGSGD RVSGSSQSPP NLKYKSTLKI KESTKERQGP DDKPPSKKSF 

       250        260        270        280        290        300 
FYKEVFESRN GDYAREGGRF FPRERKRFRG QERPDDFTAS VSEGIMTYAN SVVSDMMVSI 

       310        320        330        340        350        360 
MKTLKIQVKD TTIATILLKK VLLKHAKEVV SDLIDSFLRN LHSVTGTLMT DTQFVSAVKR 

       370        380        390        400        410        420 
TVFSHGSQKA TDIMDAMLRK LYNVMFAKKV PEHVRKAQDK AESYSLISMK GMGDPKNRNV 

       430        440        450        460        470        480 
NFAMKSETKL REKMYSEPKS EEETCAKTLG EHIIKEGLTL WHKSQQKECK SLGFQHAAFE 

       490        500        510        520        530        540 
APNTQRKPAS DISFEYPEDI GNLSLPPYPP EKPENFMYDS DSWAKDLIVS ALLLIQYHLA 

       550        560        570        580        590        600 
QGGRRDARSF VEAAGTTNFP ANEPPVAPDE SCLKSAPIVG DQEQAEKKDL RSVFFNFIRN 

       610        620        630        640        650        660 
LLSETIFKRD QSPEPKVPEQ PVKEDRKLCE RPLASSPPRL YEDDETPGAL SGLTKMAVSQ 

       670        680        690        700        710        720 
IDGHMSGQMV EHLMNSVMKL CVIIAKSCDA SLAELGDDKS GDASRLTSAF PDSLYECLPA 

       730        740        750        760        770        780 
KGTGSAEAVL QNAYQAIHNE MRGTSGQPPE GCAAPTVIVS NHNLTDTVQN KQLQAVLQWV 

       790        800        810        820        830        840 
AASELNVPIL YFAGDDEGIQ EKLLQLSAAA VDKGCSVGEV LQSVLRYEKE RQLNEAVGNV 

       850 
TPLQLLDWLM VNL

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of SOB1, a new testis-specific cDNA encoding a human sperm protein probably involved in oocyte recognition." Lefevre A., Duquenne C., Rousseau-Merck M.-F., Rogier E., Finaz C. Biochem. Biophys. Res. Commun. 259:60-66(1999) [PubMed: 10334916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Tissue: Testis.
[2]	"FSP95, a testis-specific 95-kilodalton fibrous sheath antigen that undergoes tyrosine phosphorylation in capacitated human spermatozoa." Mandal A., Naaby-Hansen S., Wolkowicz M.J., Klotz K., Shetty J., Retief J.D., Coonrod S.A., Kinter M., Sherman N., Cesar F., Flickinger C.J., Herr J.C. Biol. Reprod. 61:1184-1197(1999) [PubMed: 10529264] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, VARIANTS GLU-118 AND THR-500. Tissue: Testis.
[3]	"Isolation and molecular characterization of AKAP110, a novel, sperm-specific protein kinase A-anchoring protein." Vijayaraghavan S., Liberty G.A., Mohan J., Winfrey V.P., Olson G.E., Carr D.W. Mol. Endocrinol. 13:705-717(1999) [PubMed: 10319321] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Testis.
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT GLU-118. Tissue: Testis.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT GLU-118. Tissue: Testis.
[6]	"Identification of sperm-specific proteins that interact with A-kinase anchoring proteins in a manner similar to the type II regulatory subunit of PKA." Carr D.W., Fujita A., Stentz C.L., Liberty G.A., Olson G.E., Narumiya S. J. Biol. Chem. 276:17332-17338(2001) [PubMed: 11278869] [Abstract] Cited for: INTERACTION WITH ROPN1 AND ROPN1L, MUTAGENESIS OF LEU-131.
[7]	"Phosphoproteome analysis of capacitated human sperm. Evidence of tyrosine phosphorylation of a kinase-anchoring protein 3 and valosin-containing protein/p97 during capacitation." Ficarro S., Chertihin O., Westbrook V.A., White F., Jayes F., Kalab P., Marto J.A., Shabanowitz J., Herr J.C., Hunt D.F., Visconti P.E. J. Biol. Chem. 278:11579-11589(2003) [PubMed: 12509440] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-205; SER-208; SER-403; TYR-404; SER-440; SER-612; SER-635 AND SER-636, MASS SPECTROMETRY. Tissue: Sperm.
[8]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. Velculescu V.E. Science 314:268-274(2006) [PubMed: 16959974] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] CYS-831.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	U85715 mRNA. Translation: AAD21218.1. AF087003 mRNA. Translation: AAC35854.1. AF093408 mRNA. Translation: AAC63371.1. AK292451 mRNA. Translation: BAF85140.1. BC047535 mRNA. Translation: AAH47535.1.
IPI	IPI00290854.
RefSeq	NP_006413.2.
UniGene	Hs.98397
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	O75969.
PTM databases
PhosphoSite	O75969.
Proteomic databases
PRIDE	O75969.
Genome annotation databases
Ensembl	ENST00000228850; ENSP00000228850; ENSG00000111254; Homo sapiens. [Genome view]
GeneID	10566.
KEGG	hsa:10566.
UCSC	uc001qnb.2. human.
Organism-specific databases
CTD	10566.
GeneCards	GC12M004594.
H-InvDB	HIX0026339.
HGNC	HGNC:373. AKAP3.
MIM	604689. gene.
PharmGKB	PA24667.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	O75969.
HOVERGEN	O75969.
Gene expression databases
ArrayExpress	O75969.
Bgee	O75969.
CleanEx	HS_AKAP3.
Genevestigator	O75969.
GermOnline	ENSG00000111254. Homo sapiens.
Family and domain databases
InterPro	IPR020799. AKAP_110. IPR018292. AKAP_110_C. IPR018459. RII_binding_1. IPR008382. SPHK1-interactor_AKAP_110. [Graphical view]
PANTHER	PTHR10226. AKAP_110. 1 hit.
Pfam	PF05716. AKAP_110. 1 hit. PF10522. RII_binding_1. 1 hit. [Graphical view]
SMART	SM00807. AKAP_110. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	40101.
SOURCE	Search...

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Entry information

Entry name

AKAP3_HUMAN

Accession

Primary (citable) accession number: O75969
Secondary accession number(s): O75945, Q86X01, Q9UM61

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	November 1, 1998
Last modified:	November 24, 2009
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

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List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Natural variations

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents