O75962 (TRIO_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Triple functional domain protein
EC=2.7.11.1

Alternative name(s):
PTPRF-interacting protein

Gene names

Name:

TRIO

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	3097 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Promotes the exchange of GDP by GTP. Together with leukocyte antigen-related (LAR) protein, it could play a role in coordinating cell-matrix and cytoskeletal rearrangements necessary for cell migration and cell growth.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subunit structure	Interacts to form a complex with leukocyte antigen related protein.
Subcellular location	Cytoplasm By similarity.
Tissue specificity	Highly expressed in heart, skeletal muscle, brain, pancreas, placenta, liver, kidney and lung.
Domain	The N-terminal DBL/GEF domain specifically catalyzes nucleotide exchange for RAC1, leading to the activation of Jun kinase and the production of membrane ruffles. The second DBL/GEF domain is an exchange factor for rhoa and induces the formation of stress fibers.
Post-translational modification	Phosphorylated on serine residue(s).
Sequence similarities	Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Contains 1 CRAL-TRIO domain. Contains 2 DH (DBL-homology) domains. Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 2 PH domains. Contains 1 protein kinase domain. Contains 2 SH3 domains. Contains 4 spectrin repeats.
Sequence caution	The sequence AAC34245.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence AAC34245.1 differs from that shown. Reason: Frameshift at position 2301. The sequence AAC43042.1 differs from that shown. Reason: Erroneous initiation. Isoform 2: The sequence AAC34245.1 differs from that shown. Reason: Frameshift at position 2301.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Immunoglobulin domain Repeat SH3 domain
Ligand	ATP-binding Nucleotide-binding
Molecular function	Guanine-nucleotide releasing factor Kinase Serine/threonine-protein kinase Transferase
PTM	Disulfide bond Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	apoptotic signaling pathway Traceable author statement. Source: Reactome axon guidance Traceable author statement. Source: Reactome neurotrophin TRK receptor signaling pathway Traceable author statement. Source: Reactome regulation of Rho protein signal transduction Inferred from electronic annotation. Source: InterPro regulation of small GTPase mediated signal transduction Traceable author statement. Source: Reactome small GTPase mediated signal transduction Traceable author statement. Source: Reactome transmembrane receptor protein tyrosine phosphatase signaling pathway Traceable author statement Ref.5. Source: ProtInc
Cellular_component	cytosol Traceable author statement. Source: Reactome
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW Rho guanyl-nucleotide exchange factor activity Inferred from electronic annotation. Source: InterPro guanyl-nucleotide exchange factor activity Traceable author statement Ref.5. Source: ProtInc phospholipid binding Inferred from electronic annotation. Source: InterPro protein serine/threonine kinase activity Traceable author statement Ref.5. Source: ProtInc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
DISC1	Q9NRI5	3	EBI-718519,EBI-529989

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O75962-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O75962-2)

The sequence of this isoform differs from the canonical sequence as follows:
     2368-2368: G → E
     2369-2544: Missing.

Isoform 3 (identifier: O75962-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-2501: Missing.
     2502-2544: FPGDSDSLQR...QSVQSTQSNG → MLPSQAQGLL...LARNTFLKAC

Note: No experimental confirmation available.

Isoform 4 (identifier: O75962-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-59: Missing.

Note: No experimental confirmation available.

Isoform 5 (identifier: O75962-5)

The sequence of this isoform differs from the canonical sequence as follows:
     2545-2563: SESSSSSNISTMLVTHDYT → VSASGGPRPPAPLPLSRQL
     2564-3097: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 3097

3097

Triple functional domain protein

PRO_0000080978

Regions

Domain

65 – 210

146

CRAL-TRIO

Repeat

311 – 418

108

Spectrin 1

Repeat

538 – 644

107

Spectrin 2

Repeat

878 – 984

107

Spectrin 3

Repeat

1109 – 1216

108

Spectrin 4

Domain

1292 – 1467

176

DH 1

Domain

1480 – 1591

112

PH 1

Domain

1656 – 1712

57

SH3 1

Domain

1969 – 2145

177

DH 2

Domain

2157 – 2271

115

PH 2

Domain

2551 – 2616

66

SH3 2

Domain

2685 – 2775

91

Ig-like C2-type

Domain

2796 – 3052

257

Protein kinase

Nucleotide binding

2802 – 2810

9

ATP By similarity

Compositional bias

715 – 718

4

Poly-Gln

Compositional bias

1845 – 1850

6

Poly-Ser

Compositional bias

2292 – 2312

21

Poly-Gly

Compositional bias

2545 – 2551

7

Poly-Ser

Sites

Active site

2915

1

By similarity

Binding site

2825

1

ATP By similarity

Amino acid modifications

Modified residue

2282

1

Phosphoserine Ref.10

Modified residue

2455

1

Phosphoserine Ref.10 Ref.12

Modified residue

2459

1

Phosphoserine Ref.12

Disulfide bond

2696 ↔ 2759

Potential

Natural variations

Alternative sequence

1 – 2501

2501

Missing in isoform 3.

VSP_023306

Alternative sequence

1 – 59

59

Missing in isoform 4.

VSP_037860

Alternative sequence

2368

1

G → E in isoform 2.

VSP_004467

Alternative sequence

2369 – 2544

176

Missing in isoform 2.

VSP_004468

Alternative sequence

2502 – 2544

43

FPGDS…TQSNG → MLPSQAQGLLWWVFPLFPAS SLSYPPVSYRADGLARNTFL KAC in isoform 3.

VSP_023307

Alternative sequence

2545 – 2563

19

SESSS…THDYT → VSASGGPRPPAPLPLSRQL in isoform 5.

VSP_037861

Alternative sequence

2564 – 3097

534

Missing in isoform 5.

VSP_037862

Natural variant

291

1

S → T. Ref.15
Corresponds to variant rs55772118 [ dbSNP | Ensembl ].

VAR_041899

Natural variant

348

1

D → E.
Corresponds to variant rs16903367 [ dbSNP | Ensembl ].

VAR_059802

Natural variant

1613

1

A → T.
Corresponds to variant rs16903474 [ dbSNP | Ensembl ].

VAR_059803

Natural variant

1644

1

T → M. Ref.15
Corresponds to variant rs55687522 [ dbSNP | Ensembl ].

VAR_041900

Natural variant

1690

1

H → R. Ref.15
Corresponds to variant rs56292586 [ dbSNP | Ensembl ].

VAR_041901

Natural variant

1978

1

V → M in a metastatic melanoma sample; somatic mutation. Ref.15

VAR_041902

Natural variant

2242

1

T → M. Ref.15
Corresponds to variant rs55916212 [ dbSNP | Ensembl ].

VAR_041903

Experimental info

Mutagenesis

1299

1

E → A: 50% decrease in nucleotide exchange activity.

Mutagenesis

1303

1

T → A: 40% decrease in nucleotide exchange activity.

Mutagenesis

1389

1

N → A: No change in nucleotide exchange activity.

Mutagenesis

1426

1

V → A: 90% decrease in nucleotide exchange activity.

Mutagenesis

1427

1

Q → A: 80% decrease in nucleotide exchange activity.

Mutagenesis

1428

1

R → A: 80% decrease in nucleotide exchange activity.

Mutagenesis

1430

1

T → A: 80% decrease in nucleotide exchange activity.

Mutagenesis

1431

1

K → A: Loss of nucleotide exchange activity.

Mutagenesis

1434

1

L → A: 40% decrease in nucleotide exchange activity.

Mutagenesis

1437

1

K → A: No change in nucleotide exchange activity.

Mutagenesis

1438

1

E → A: 30% decrease in nucleotide exchange activity.

Sequence conflict

550 – 553

4

QLEN → HVRT in AAC34245. Ref.5

Sequence conflict

550 – 553

4

QLEN → HVRT in AAC43042. Ref.6

Sequence conflict

574

1

D → E in AAC34245. Ref.5

Sequence conflict

574

1

D → E in AAC43042. Ref.6

Sequence conflict

787 – 788

2

QL → HV in AAC34245. Ref.5

Sequence conflict

787 – 788

2

QL → HV in AAC43042. Ref.6

Secondary structure

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3097

Helix Strand Turn

Details...

Helix

1291 – 1316

26

Helix

1318 – 1324

7

Turn

1331 – 1335

5

Helix

1337 – 1341

5

Helix

1344 – 1353

10

Helix

1355 – 1361

7

Turn

1362 – 1364

3

Helix

1366 – 1369

4

Helix

1370 – 1375

6

Turn

1376 – 1380

5

Helix

1381 – 1400

20

Turn

1401 – 1403

3

Helix

1404 – 1412

9

Helix

1418 – 1422

5

Helix

1424 – 1441

18

Helix

1450 – 1470

21

Beta strand

1473 – 1475

3

Helix

1481 – 1483

3

Beta strand

1486 – 1495

10

Beta strand

1497 – 1501

5

Beta strand

1504 – 1523

20

Beta strand

1529 – 1538

10

Helix

1539 – 1541

3

Beta strand

1542 – 1545

4

Beta strand

1549 – 1552

4

Beta strand

1554 – 1562

9

Turn

1565 – 1567

3

Beta strand

1569 – 1572

4

Helix

1576 – 1592

17

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified September 1, 2009. Version 2. Checksum: EA9236DF88B0EA24	FASTA	3,097	346,900
	10 20 30 40 50 60 MSGSSGGAAA PAASSGPAAA ASAAGSGCGG GAGEGAEEAA KDLADIAAFF RSGFRKNDEM 70 80 90 100 110 120 KAMDVLPILK EKVAYLSGGR DKRGGPILTF PARSNHDRIR QEDLRRLISY LACIPSEEVC 130 140 150 160 170 180 KRGFTVIVDM RGSKWDSIKP LLKILQESFP CCIHVALIIK PDNFWQKQRT NFGSSKFEFE 190 200 210 220 230 240 TNMVSLEGLT KVVDPSQLTP EFDGCLEYNH EEWIEIRVAF EDYISNATHM LSRLEELQDI 250 260 270 280 290 300 LAKKELPQDL EGARNMIEEH SQLKKKVIKA PIEDLDLEGQ KLLQRIQSSE SFPKKNSGSG 310 320 330 340 350 360 NADLQNLLPK VSTMLDRLHS TRQHLHQMWH VRKLKLDQCF QLRLFEQDAE KMFDWITHNK 370 380 390 400 410 420 GLFLNSYTEI GTSHPHAMEL QTQHNHFAMN CMNVYVNINR IMSVANRLVE SGHYASQQIR 430 440 450 460 470 480 QIASQLEQEW KAFAAALDER STLLDMSSIF HQKAEKYMSN VDSWCKACGE VDLPSELQDL 490 500 510 520 530 540 EDAIHHHQGI YEHITLAYSE VSQDGKSLLD KLQRPLTPGS SDSLTASANY SKAVHHVLDV 550 560 570 580 590 600 IHEVLHHQRQ LENIWQHRKV RLHQRLQLCV FQQDVQQVLD WIENHGEAFL SKHTGVGKSL 610 620 630 640 650 660 HRARALQKRH EDFEEVAQNT YTNADKLLEA AEQLAQTGEC DPEEIYQAAH QLEDRIQDFV 670 680 690 700 710 720 RRVEQRKILL DMSVSFHTHV KELWTWLEEL QKELLDDVYA ESVEAVQDLI KRFGQQQQTT 730 740 750 760 770 780 LQVTVNVIKE GEDLIQQLRD SAISSNKTPH NSSINHIETV LQQLDEAQSQ MEELFQERKI 790 800 810 820 830 840 KLELFLQLRI FERDAIDIIS DLESWNDELS QQMNDFDTED LTIAEQRLQH HADKALTMNN 850 860 870 880 890 900 LTFDVIHQGQ DLLQYVNEVQ ASGVELLCDR DVDMATRVQD LLEFLHEKQQ ELDLAAEQHR 910 920 930 940 950 960 KHLEQCVQLR HLQAEVKQVL GWIRNGESML NAGLITASSL QEAEQLQREH EQFQHAIEKT 970 980 990 1000 1010 1020 HQSALQVQQK AEAMLQANHY DMDMIRDCAE KVASHWQQLM LKMEDRLKLV NASVAFYKTS 1030 1040 1050 1060 1070 1080 EQVCSVLESL EQEYKREEDW CGGADKLGPN SETDHVTPMI SKHLEQKEAF LKACTLARRN 1090 1100 1110 1120 1130 1140 ADVFLKYLHR NSVNMPGMVT HIKAPEQQVK NILNELFQRE NRVLHYWTMR KRRLDQCQQY 1150 1160 1170 1180 1190 1200 VVFERSAKQA LEWIHDNGEF YLSTHTSTGS SIQHTQELLK EHEEFQITAK QTKERVKLLI 1210 1220 1230 1240 1250 1260 QLADGFCEKG HAHAAEIKKC VTAVDKRYRD FSLRMEKYRT SLEKALGISS DSNKSSKSLQ 1270 1280 1290 1300 1310 1320 LDIIPASIPG SEVKLRDAAH ELNEEKRKSA RRKEFIMAEL IQTEKAYVRD LRECMDTYLW 1330 1340 1350 1360 1370 1380 EMTSGVEEIP PGIVNKELII FGNMQEIYEF HNNIFLKELE KYEQLPEDVG HCFVTWADKF 1390 1400 1410 1420 1430 1440 QMYVTYCKNK PDSTQLILEH AGSYFDEIQQ RHGLANSISS YLIKPVQRIT KYQLLLKELL 1450 1460 1470 1480 1490 1500 TCCEEGKGEI KDGLEVMLSV PKRANDAMHL SMLEGFDENI ESQGELILQE SFQVWDPKTL 1510 1520 1530 1540 1550 1560 IRKGRERHLF LFEMSLVFSK EVKDSSGRSK YLYKSKLFTS ELGVTEHVEG DPCKFALWVG 1570 1580 1590 1600 1610 1620 RTPTSDNKIV LKASSIENKQ DWIKHIREVI QERTIHLKGA LKEPIHIPKT APATRQKGRR 1630 1640 1650 1660 1670 1680 DGEDLDSQGD GSSQPDTISI ASRTSQNTLD SDKLSGGCEL TVVIHDFTAC NSNELTIRRG 1690 1700 1710 1720 1730 1740 QTVEVLERPH DKPDWCLVRT TDRSPAAEGL VPCGSLCIAH SRSSMEMEGI FNHKDSLSVS 1750 1760 1770 1780 1790 1800 SNDASPPASV ASLQPHMIGA QSSPGPKRPG NTLRKWLTSP VRRLSSGKAD GHVKKLAHKH 1810 1820 1830 1840 1850 1860 KKSREVRKSA DAGSQKDSDD SAATPQDETV EERGRNEGLS SGTLSKSSSS GMQSCGEEEG 1870 1880 1890 1900 1910 1920 EEGADAVPLP PPMAIQQHSL LQPDSQDDKA SSRLLVRPTS SETPSAAELV SAIEELVKSK 1930 1940 1950 1960 1970 1980 MALEDRPSSL LVDQGDSSSP SFNPSDNSLL SSSSPIDEME ERKSSSLKRR HYVLQELVET 1990 2000 2010 2020 2030 2040 ERDYVRDLGY VVEGYMALMK EDGVPDDMKG KDKIVFGNIH QIYDWHRDFF LGELEKCLED 2050 2060 2070 2080 2090 2100 PEKLGSLFVK HERRLHMYIA YCQNKPKSEH IVSEYIDTFF EDLKQRLGHR LQLTDLLIKP 2110 2120 2130 2140 2150 2160 VQRIMKYQLL LKDFLKYSKK ASLDTSELER AVEVMCIVPR RCNDMMNVGR LQGFDGKIVA 2170 2180 2190 2200 2210 2220 QGKLLLQDTF LVTDQDAGLL PRCRERRIFL FEQIVIFSEP LDKKKGFSMP GFLFKNSIKV 2230 2240 2250 2260 2270 2280 SCLCLEENVE NDPCKFALTS RTGDVVETFI LHSSSPSVRQ TWIHEINQIL ENQRNFLNAL 2290 2300 2310 2320 2330 2340 TSPIEYQRNH SGGGGGGGSG GSGGGGGSGG GGAPSGGSGH SGGPSSCGGA PSTSRSRPSR 2350 2360 2370 2380 2390 2400 IPQPVRHHPP VLVSSAASSQ AEADKMSGTS TPGPSLPPPG AAPEAGPSAP SRRPPGADAE 2410 2420 2430 2440 2450 2460 GSEREAEPIP KMKVLESPRK GAANASGSSP DAPAKDARAS LGTLPLGKPR AGAASPLNSP 2470 2480 2490 2500 2510 2520 LSSAVPSLGK EPFPPSSPLQ KGGSFWSSIP ASPASRPGSF TFPGDSDSLQ RQTPRHAAPG 2530 2540 2550 2560 2570 2580 KDTDRMSTCS SASEQSVQST QSNGSESSSS SNISTMLVTH DYTAVKEDEI NVYQGEVVQI 2590 2600 2610 2620 2630 2640 LASNQQNMFL VFRAATDQCP AAEGWIPGFV LGHTSAVIVE NPDGTLKKST SWHTALRLRK 2650 2660 2670 2680 2690 2700 KSEKKDKDGK REGKLENGYR KSREGLSNKV SVKLLNPNYI YDVPPEFVIP LSEVTCETGE 2710 2720 2730 2740 2750 2760 TVVLRCRVCG RPKASITWKG PEHNTLNNDG HYSISYSDLG EATLKIVGVT TEDDGIYTCI 2770 2780 2790 2800 2810 2820 AVNDMGSASS SASLRVLGPG MDGIMVTWKD NFDSFYSEVA ELGRGRFSVV KKCDQKGTKR 2830 2840 2850 2860 2870 2880 AVATKFVNKK LMKRDQVTHE LGILQSLQHP LLVGLLDTFE TPTSYILVLE MADQGRLLDC 2890 2900 2910 2920 2930 2940 VVRWGSLTEG KIRAHLGEVL EAVRYLHNCR IAHLDLKPEN ILVDESLAKP TIKLADFGDA 2950 2960 2970 2980 2990 3000 VQLNTTYYIH QLLGNPEFAA PEIILGNPVS LTSDTWSVGV LTYVLLSGVS PFLDDSVEET 3010 3020 3030 3040 3050 3060 CLNICRLDFS FPDDYFKGVS QKAKEFVCFL LQEDPAKRPS AALALQEQWL QAGNGRSTGV 3070 3080 3090 LDTSRLTSFI ERRKHQNDVR PIRSIKNFLQ SRLLPRV
	Isoform 2 [UniParc]. Checksum: 7F2AEF630EF984C9	FASTA	2,921	329,389
	10 20 30 40 50 60 MSGSSGGAAA PAASSGPAAA ASAAGSGCGG GAGEGAEEAA KDLADIAAFF RSGFRKNDEM 70 80 90 100 110 120 KAMDVLPILK EKVAYLSGGR DKRGGPILTF PARSNHDRIR QEDLRRLISY LACIPSEEVC 130 140 150 160 170 180 KRGFTVIVDM RGSKWDSIKP LLKILQESFP CCIHVALIIK PDNFWQKQRT NFGSSKFEFE 190 200 210 220 230 240 TNMVSLEGLT KVVDPSQLTP EFDGCLEYNH EEWIEIRVAF EDYISNATHM LSRLEELQDI 250 260 270 280 290 300 LAKKELPQDL EGARNMIEEH SQLKKKVIKA PIEDLDLEGQ KLLQRIQSSE SFPKKNSGSG 310 320 330 340 350 360 NADLQNLLPK VSTMLDRLHS TRQHLHQMWH VRKLKLDQCF QLRLFEQDAE KMFDWITHNK 370 380 390 400 410 420 GLFLNSYTEI GTSHPHAMEL QTQHNHFAMN CMNVYVNINR IMSVANRLVE SGHYASQQIR 430 440 450 460 470 480 QIASQLEQEW KAFAAALDER STLLDMSSIF HQKAEKYMSN VDSWCKACGE VDLPSELQDL 490 500 510 520 530 540 EDAIHHHQGI YEHITLAYSE VSQDGKSLLD KLQRPLTPGS SDSLTASANY SKAVHHVLDV 550 560 570 580 590 600 IHEVLHHQRQ LENIWQHRKV RLHQRLQLCV FQQDVQQVLD WIENHGEAFL SKHTGVGKSL 610 620 630 640 650 660 HRARALQKRH EDFEEVAQNT YTNADKLLEA AEQLAQTGEC DPEEIYQAAH QLEDRIQDFV 670 680 690 700 710 720 RRVEQRKILL DMSVSFHTHV KELWTWLEEL QKELLDDVYA ESVEAVQDLI KRFGQQQQTT 730 740 750 760 770 780 LQVTVNVIKE GEDLIQQLRD SAISSNKTPH NSSINHIETV LQQLDEAQSQ MEELFQERKI 790 800 810 820 830 840 KLELFLQLRI FERDAIDIIS DLESWNDELS QQMNDFDTED LTIAEQRLQH HADKALTMNN 850 860 870 880 890 900 LTFDVIHQGQ DLLQYVNEVQ ASGVELLCDR DVDMATRVQD LLEFLHEKQQ ELDLAAEQHR 910 920 930 940 950 960 KHLEQCVQLR HLQAEVKQVL GWIRNGESML NAGLITASSL QEAEQLQREH EQFQHAIEKT 970 980 990 1000 1010 1020 HQSALQVQQK AEAMLQANHY DMDMIRDCAE KVASHWQQLM LKMEDRLKLV NASVAFYKTS 1030 1040 1050 1060 1070 1080 EQVCSVLESL EQEYKREEDW CGGADKLGPN SETDHVTPMI SKHLEQKEAF LKACTLARRN 1090 1100 1110 1120 1130 1140 ADVFLKYLHR NSVNMPGMVT HIKAPEQQVK NILNELFQRE NRVLHYWTMR KRRLDQCQQY 1150 1160 1170 1180 1190 1200 VVFERSAKQA LEWIHDNGEF YLSTHTSTGS SIQHTQELLK EHEEFQITAK QTKERVKLLI 1210 1220 1230 1240 1250 1260 QLADGFCEKG HAHAAEIKKC VTAVDKRYRD FSLRMEKYRT SLEKALGISS DSNKSSKSLQ 1270 1280 1290 1300 1310 1320 LDIIPASIPG SEVKLRDAAH ELNEEKRKSA RRKEFIMAEL IQTEKAYVRD LRECMDTYLW 1330 1340 1350 1360 1370 1380 EMTSGVEEIP PGIVNKELII FGNMQEIYEF HNNIFLKELE KYEQLPEDVG HCFVTWADKF 1390 1400 1410 1420 1430 1440 QMYVTYCKNK PDSTQLILEH AGSYFDEIQQ RHGLANSISS YLIKPVQRIT KYQLLLKELL 1450 1460 1470 1480 1490 1500 TCCEEGKGEI KDGLEVMLSV PKRANDAMHL SMLEGFDENI ESQGELILQE SFQVWDPKTL 1510 1520 1530 1540 1550 1560 IRKGRERHLF LFEMSLVFSK EVKDSSGRSK YLYKSKLFTS ELGVTEHVEG DPCKFALWVG 1570 1580 1590 1600 1610 1620 RTPTSDNKIV LKASSIENKQ DWIKHIREVI QERTIHLKGA LKEPIHIPKT APATRQKGRR 1630 1640 1650 1660 1670 1680 DGEDLDSQGD GSSQPDTISI ASRTSQNTLD SDKLSGGCEL TVVIHDFTAC NSNELTIRRG 1690 1700 1710 1720 1730 1740 QTVEVLERPH DKPDWCLVRT TDRSPAAEGL VPCGSLCIAH SRSSMEMEGI FNHKDSLSVS 1750 1760 1770 1780 1790 1800 SNDASPPASV ASLQPHMIGA QSSPGPKRPG NTLRKWLTSP VRRLSSGKAD GHVKKLAHKH 1810 1820 1830 1840 1850 1860 KKSREVRKSA DAGSQKDSDD SAATPQDETV EERGRNEGLS SGTLSKSSSS GMQSCGEEEG 1870 1880 1890 1900 1910 1920 EEGADAVPLP PPMAIQQHSL LQPDSQDDKA SSRLLVRPTS SETPSAAELV SAIEELVKSK 1930 1940 1950 1960 1970 1980 MALEDRPSSL LVDQGDSSSP SFNPSDNSLL SSSSPIDEME ERKSSSLKRR HYVLQELVET 1990 2000 2010 2020 2030 2040 ERDYVRDLGY VVEGYMALMK EDGVPDDMKG KDKIVFGNIH QIYDWHRDFF LGELEKCLED 2050 2060 2070 2080 2090 2100 PEKLGSLFVK HERRLHMYIA YCQNKPKSEH IVSEYIDTFF EDLKQRLGHR LQLTDLLIKP 2110 2120 2130 2140 2150 2160 VQRIMKYQLL LKDFLKYSKK ASLDTSELER AVEVMCIVPR RCNDMMNVGR LQGFDGKIVA 2170 2180 2190 2200 2210 2220 QGKLLLQDTF LVTDQDAGLL PRCRERRIFL FEQIVIFSEP LDKKKGFSMP GFLFKNSIKV 2230 2240 2250 2260 2270 2280 SCLCLEENVE NDPCKFALTS RTGDVVETFI LHSSSPSVRQ TWIHEINQIL ENQRNFLNAL 2290 2300 2310 2320 2330 2340 TSPIEYQRNH SGGGGGGGSG GSGGGGGSGG GGAPSGGSGH SGGPSSCGGA PSTSRSRPSR 2350 2360 2370 2380 2390 2400 IPQPVRHHPP VLVSSAASSQ AEADKMSESE SSSSSNISTM LVTHDYTAVK EDEINVYQGE 2410 2420 2430 2440 2450 2460 VVQILASNQQ NMFLVFRAAT DQCPAAEGWI PGFVLGHTSA VIVENPDGTL KKSTSWHTAL 2470 2480 2490 2500 2510 2520 RLRKKSEKKD KDGKREGKLE NGYRKSREGL SNKVSVKLLN PNYIYDVPPE FVIPLSEVTC 2530 2540 2550 2560 2570 2580 ETGETVVLRC RVCGRPKASI TWKGPEHNTL NNDGHYSISY SDLGEATLKI VGVTTEDDGI 2590 2600 2610 2620 2630 2640 YTCIAVNDMG SASSSASLRV LGPGMDGIMV TWKDNFDSFY SEVAELGRGR FSVVKKCDQK 2650 2660 2670 2680 2690 2700 GTKRAVATKF VNKKLMKRDQ VTHELGILQS LQHPLLVGLL DTFETPTSYI LVLEMADQGR 2710 2720 2730 2740 2750 2760 LLDCVVRWGS LTEGKIRAHL GEVLEAVRYL HNCRIAHLDL KPENILVDES LAKPTIKLAD 2770 2780 2790 2800 2810 2820 FGDAVQLNTT YYIHQLLGNP EFAAPEIILG NPVSLTSDTW SVGVLTYVLL SGVSPFLDDS 2830 2840 2850 2860 2870 2880 VEETCLNICR LDFSFPDDYF KGVSQKAKEF VCFLLQEDPA KRPSAALALQ EQWLQAGNGR 2890 2900 2910 2920 STGVLDTSRL TSFIERRKHQ NDVRPIRSIK NFLQSRLLPR V
	Isoform 3 [UniParc]. Checksum: F6DA7D25AE9E7F0C	FASTA	596	66,206
	10 20 30 40 50 60 MLPSQAQGLL WWVFPLFPAS SLSYPPVSYR ADGLARNTFL KACSESSSSS NISTMLVTHD 70 80 90 100 110 120 YTAVKEDEIN VYQGEVVQIL ASNQQNMFLV FRAATDQCPA AEGWIPGFVL GHTSAVIVEN 130 140 150 160 170 180 PDGTLKKSTS WHTALRLRKK SEKKDKDGKR EGKLENGYRK SREGLSNKVS VKLLNPNYIY 190 200 210 220 230 240 DVPPEFVIPL SEVTCETGET VVLRCRVCGR PKASITWKGP EHNTLNNDGH YSISYSDLGE 250 260 270 280 290 300 ATLKIVGVTT EDDGIYTCIA VNDMGSASSS ASLRVLGPGM DGIMVTWKDN FDSFYSEVAE 310 320 330 340 350 360 LGRGRFSVVK KCDQKGTKRA VATKFVNKKL MKRDQVTHEL GILQSLQHPL LVGLLDTFET 370 380 390 400 410 420 PTSYILVLEM ADQGRLLDCV VRWGSLTEGK IRAHLGEVLE AVRYLHNCRI AHLDLKPENI 430 440 450 460 470 480 LVDESLAKPT IKLADFGDAV QLNTTYYIHQ LLGNPEFAAP EIILGNPVSL TSDTWSVGVL 490 500 510 520 530 540 TYVLLSGVSP FLDDSVEETC LNICRLDFSF PDDYFKGVSQ KAKEFVCFLL QEDPAKRPSA 550 560 570 580 590 ALALQEQWLQ AGNGRSTGVL DTSRLTSFIE RRKHQNDVRP IRSIKNFLQS RLLPRV
	Isoform 4 [UniParc]. Checksum: 2E9F96D84514638C	FASTA	3,038	341,598
	10 20 30 40 50 60 MKAMDVLPIL KEKVAYLSGG RDKRGGPILT FPARSNHDRI RQEDLRRLIS YLACIPSEEV 70 80 90 100 110 120 CKRGFTVIVD MRGSKWDSIK PLLKILQESF PCCIHVALII KPDNFWQKQR TNFGSSKFEF 130 140 150 160 170 180 ETNMVSLEGL TKVVDPSQLT PEFDGCLEYN HEEWIEIRVA FEDYISNATH MLSRLEELQD 190 200 210 220 230 240 ILAKKELPQD LEGARNMIEE HSQLKKKVIK APIEDLDLEG QKLLQRIQSS ESFPKKNSGS 250 260 270 280 290 300 GNADLQNLLP KVSTMLDRLH STRQHLHQMW HVRKLKLDQC FQLRLFEQDA EKMFDWITHN 310 320 330 340 350 360 KGLFLNSYTE IGTSHPHAME LQTQHNHFAM NCMNVYVNIN RIMSVANRLV ESGHYASQQI 370 380 390 400 410 420 RQIASQLEQE WKAFAAALDE RSTLLDMSSI FHQKAEKYMS NVDSWCKACG EVDLPSELQD 430 440 450 460 470 480 LEDAIHHHQG IYEHITLAYS EVSQDGKSLL DKLQRPLTPG SSDSLTASAN YSKAVHHVLD 490 500 510 520 530 540 VIHEVLHHQR QLENIWQHRK VRLHQRLQLC VFQQDVQQVL DWIENHGEAF LSKHTGVGKS 550 560 570 580 590 600 LHRARALQKR HEDFEEVAQN TYTNADKLLE AAEQLAQTGE CDPEEIYQAA HQLEDRIQDF 610 620 630 640 650 660 VRRVEQRKIL LDMSVSFHTH VKELWTWLEE LQKELLDDVY AESVEAVQDL IKRFGQQQQT 670 680 690 700 710 720 TLQVTVNVIK EGEDLIQQLR DSAISSNKTP HNSSINHIET VLQQLDEAQS QMEELFQERK 730 740 750 760 770 780 IKLELFLQLR IFERDAIDII SDLESWNDEL SQQMNDFDTE DLTIAEQRLQ HHADKALTMN 790 800 810 820 830 840 NLTFDVIHQG QDLLQYVNEV QASGVELLCD RDVDMATRVQ DLLEFLHEKQ QELDLAAEQH 850 860 870 880 890 900 RKHLEQCVQL RHLQAEVKQV LGWIRNGESM LNAGLITASS LQEAEQLQRE HEQFQHAIEK 910 920 930 940 950 960 THQSALQVQQ KAEAMLQANH YDMDMIRDCA EKVASHWQQL MLKMEDRLKL VNASVAFYKT 970 980 990 1000 1010 1020 SEQVCSVLES LEQEYKREED WCGGADKLGP NSETDHVTPM ISKHLEQKEA FLKACTLARR 1030 1040 1050 1060 1070 1080 NADVFLKYLH RNSVNMPGMV THIKAPEQQV KNILNELFQR ENRVLHYWTM RKRRLDQCQQ 1090 1100 1110 1120 1130 1140 YVVFERSAKQ ALEWIHDNGE FYLSTHTSTG SSIQHTQELL KEHEEFQITA KQTKERVKLL 1150 1160 1170 1180 1190 1200 IQLADGFCEK GHAHAAEIKK CVTAVDKRYR DFSLRMEKYR TSLEKALGIS SDSNKSSKSL 1210 1220 1230 1240 1250 1260 QLDIIPASIP GSEVKLRDAA HELNEEKRKS ARRKEFIMAE LIQTEKAYVR DLRECMDTYL 1270 1280 1290 1300 1310 1320 WEMTSGVEEI PPGIVNKELI IFGNMQEIYE FHNNIFLKEL EKYEQLPEDV GHCFVTWADK 1330 1340 1350 1360 1370 1380 FQMYVTYCKN KPDSTQLILE HAGSYFDEIQ QRHGLANSIS SYLIKPVQRI TKYQLLLKEL 1390 1400 1410 1420 1430 1440 LTCCEEGKGE IKDGLEVMLS VPKRANDAMH LSMLEGFDEN IESQGELILQ ESFQVWDPKT 1450 1460 1470 1480 1490 1500 LIRKGRERHL FLFEMSLVFS KEVKDSSGRS KYLYKSKLFT SELGVTEHVE GDPCKFALWV 1510 1520 1530 1540 1550 1560 GRTPTSDNKI VLKASSIENK QDWIKHIREV IQERTIHLKG ALKEPIHIPK TAPATRQKGR 1570 1580 1590 1600 1610 1620 RDGEDLDSQG DGSSQPDTIS IASRTSQNTL DSDKLSGGCE LTVVIHDFTA CNSNELTIRR 1630 1640 1650 1660 1670 1680 GQTVEVLERP HDKPDWCLVR TTDRSPAAEG LVPCGSLCIA HSRSSMEMEG IFNHKDSLSV 1690 1700 1710 1720 1730 1740 SSNDASPPAS VASLQPHMIG AQSSPGPKRP GNTLRKWLTS PVRRLSSGKA DGHVKKLAHK 1750 1760 1770 1780 1790 1800 HKKSREVRKS ADAGSQKDSD DSAATPQDET VEERGRNEGL SSGTLSKSSS SGMQSCGEEE 1810 1820 1830 1840 1850 1860 GEEGADAVPL PPPMAIQQHS LLQPDSQDDK ASSRLLVRPT SSETPSAAEL VSAIEELVKS 1870 1880 1890 1900 1910 1920 KMALEDRPSS LLVDQGDSSS PSFNPSDNSL LSSSSPIDEM EERKSSSLKR RHYVLQELVE 1930 1940 1950 1960 1970 1980 TERDYVRDLG YVVEGYMALM KEDGVPDDMK GKDKIVFGNI HQIYDWHRDF FLGELEKCLE 1990 2000 2010 2020 2030 2040 DPEKLGSLFV KHERRLHMYI AYCQNKPKSE HIVSEYIDTF FEDLKQRLGH RLQLTDLLIK 2050 2060 2070 2080 2090 2100 PVQRIMKYQL LLKDFLKYSK KASLDTSELE RAVEVMCIVP RRCNDMMNVG RLQGFDGKIV 2110 2120 2130 2140 2150 2160 AQGKLLLQDT FLVTDQDAGL LPRCRERRIF LFEQIVIFSE PLDKKKGFSM PGFLFKNSIK 2170 2180 2190 2200 2210 2220 VSCLCLEENV ENDPCKFALT SRTGDVVETF ILHSSSPSVR QTWIHEINQI LENQRNFLNA 2230 2240 2250 2260 2270 2280 LTSPIEYQRN HSGGGGGGGS GGSGGGGGSG GGGAPSGGSG HSGGPSSCGG APSTSRSRPS 2290 2300 2310 2320 2330 2340 RIPQPVRHHP PVLVSSAASS QAEADKMSGT STPGPSLPPP GAAPEAGPSA PSRRPPGADA 2350 2360 2370 2380 2390 2400 EGSEREAEPI PKMKVLESPR KGAANASGSS PDAPAKDARA SLGTLPLGKP RAGAASPLNS 2410 2420 2430 2440 2450 2460 PLSSAVPSLG KEPFPPSSPL QKGGSFWSSI PASPASRPGS FTFPGDSDSL QRQTPRHAAP 2470 2480 2490 2500 2510 2520 GKDTDRMSTC SSASEQSVQS TQSNGSESSS SSNISTMLVT HDYTAVKEDE INVYQGEVVQ 2530 2540 2550 2560 2570 2580 ILASNQQNMF LVFRAATDQC PAAEGWIPGF VLGHTSAVIV ENPDGTLKKS TSWHTALRLR 2590 2600 2610 2620 2630 2640 KKSEKKDKDG KREGKLENGY RKSREGLSNK VSVKLLNPNY IYDVPPEFVI PLSEVTCETG 2650 2660 2670 2680 2690 2700 ETVVLRCRVC GRPKASITWK GPEHNTLNND GHYSISYSDL GEATLKIVGV TTEDDGIYTC 2710 2720 2730 2740 2750 2760 IAVNDMGSAS SSASLRVLGP GMDGIMVTWK DNFDSFYSEV AELGRGRFSV VKKCDQKGTK 2770 2780 2790 2800 2810 2820 RAVATKFVNK KLMKRDQVTH ELGILQSLQH PLLVGLLDTF ETPTSYILVL EMADQGRLLD 2830 2840 2850 2860 2870 2880 CVVRWGSLTE GKIRAHLGEV LEAVRYLHNC RIAHLDLKPE NILVDESLAK PTIKLADFGD 2890 2900 2910 2920 2930 2940 AVQLNTTYYI HQLLGNPEFA APEIILGNPV SLTSDTWSVG VLTYVLLSGV SPFLDDSVEE 2950 2960 2970 2980 2990 3000 TCLNICRLDF SFPDDYFKGV SQKAKEFVCF LLQEDPAKRP SAALALQEQW LQAGNGRSTG 3010 3020 3030 VLDTSRLTSF IERRKHQNDV RPIRSIKNFL QSRLLPRV
	Isoform 5 [UniParc]. Checksum: D55716EE24B33EFE	FASTA	2,563	287,377
	10 20 30 40 50 60 MSGSSGGAAA PAASSGPAAA ASAAGSGCGG GAGEGAEEAA KDLADIAAFF RSGFRKNDEM 70 80 90 100 110 120 KAMDVLPILK EKVAYLSGGR DKRGGPILTF PARSNHDRIR QEDLRRLISY LACIPSEEVC 130 140 150 160 170 180 KRGFTVIVDM RGSKWDSIKP LLKILQESFP CCIHVALIIK PDNFWQKQRT NFGSSKFEFE 190 200 210 220 230 240 TNMVSLEGLT KVVDPSQLTP EFDGCLEYNH EEWIEIRVAF EDYISNATHM LSRLEELQDI 250 260 270 280 290 300 LAKKELPQDL EGARNMIEEH SQLKKKVIKA PIEDLDLEGQ KLLQRIQSSE SFPKKNSGSG 310 320 330 340 350 360 NADLQNLLPK VSTMLDRLHS TRQHLHQMWH VRKLKLDQCF QLRLFEQDAE KMFDWITHNK 370 380 390 400 410 420 GLFLNSYTEI GTSHPHAMEL QTQHNHFAMN CMNVYVNINR IMSVANRLVE SGHYASQQIR 430 440 450 460 470 480 QIASQLEQEW KAFAAALDER STLLDMSSIF HQKAEKYMSN VDSWCKACGE VDLPSELQDL 490 500 510 520 530 540 EDAIHHHQGI YEHITLAYSE VSQDGKSLLD KLQRPLTPGS SDSLTASANY SKAVHHVLDV 550 560 570 580 590 600 IHEVLHHQRQ LENIWQHRKV RLHQRLQLCV FQQDVQQVLD WIENHGEAFL SKHTGVGKSL 610 620 630 640 650 660 HRARALQKRH EDFEEVAQNT YTNADKLLEA AEQLAQTGEC DPEEIYQAAH QLEDRIQDFV 670 680 690 700 710 720 RRVEQRKILL DMSVSFHTHV KELWTWLEEL QKELLDDVYA ESVEAVQDLI KRFGQQQQTT 730 740 750 760 770 780 LQVTVNVIKE GEDLIQQLRD SAISSNKTPH NSSINHIETV LQQLDEAQSQ MEELFQERKI 790 800 810 820 830 840 KLELFLQLRI FERDAIDIIS DLESWNDELS QQMNDFDTED LTIAEQRLQH HADKALTMNN 850 860 870 880 890 900 LTFDVIHQGQ DLLQYVNEVQ ASGVELLCDR DVDMATRVQD LLEFLHEKQQ ELDLAAEQHR 910 920 930 940 950 960 KHLEQCVQLR HLQAEVKQVL GWIRNGESML NAGLITASSL QEAEQLQREH EQFQHAIEKT 970 980 990 1000 1010 1020 HQSALQVQQK AEAMLQANHY DMDMIRDCAE KVASHWQQLM LKMEDRLKLV NASVAFYKTS 1030 1040 1050 1060 1070 1080 EQVCSVLESL EQEYKREEDW CGGADKLGPN SETDHVTPMI SKHLEQKEAF LKACTLARRN 1090 1100 1110 1120 1130 1140 ADVFLKYLHR NSVNMPGMVT HIKAPEQQVK NILNELFQRE NRVLHYWTMR KRRLDQCQQY 1150 1160 1170 1180 1190 1200 VVFERSAKQA LEWIHDNGEF YLSTHTSTGS SIQHTQELLK EHEEFQITAK QTKERVKLLI 1210 1220 1230 1240 1250 1260 QLADGFCEKG HAHAAEIKKC VTAVDKRYRD FSLRMEKYRT SLEKALGISS DSNKSSKSLQ 1270 1280 1290 1300 1310 1320 LDIIPASIPG SEVKLRDAAH ELNEEKRKSA RRKEFIMAEL IQTEKAYVRD LRECMDTYLW 1330 1340 1350 1360 1370 1380 EMTSGVEEIP PGIVNKELII FGNMQEIYEF HNNIFLKELE KYEQLPEDVG HCFVTWADKF 1390 1400 1410 1420 1430 1440 QMYVTYCKNK PDSTQLILEH AGSYFDEIQQ RHGLANSISS YLIKPVQRIT KYQLLLKELL 1450 1460 1470 1480 1490 1500 TCCEEGKGEI KDGLEVMLSV PKRANDAMHL SMLEGFDENI ESQGELILQE SFQVWDPKTL 1510 1520 1530 1540 1550 1560 IRKGRERHLF LFEMSLVFSK EVKDSSGRSK YLYKSKLFTS ELGVTEHVEG DPCKFALWVG 1570 1580 1590 1600 1610 1620 RTPTSDNKIV LKASSIENKQ DWIKHIREVI QERTIHLKGA LKEPIHIPKT APATRQKGRR 1630 1640 1650 1660 1670 1680 DGEDLDSQGD GSSQPDTISI ASRTSQNTLD SDKLSGGCEL TVVIHDFTAC NSNELTIRRG 1690 1700 1710 1720 1730 1740 QTVEVLERPH DKPDWCLVRT TDRSPAAEGL VPCGSLCIAH SRSSMEMEGI FNHKDSLSVS 1750 1760 1770 1780 1790 1800 SNDASPPASV ASLQPHMIGA QSSPGPKRPG NTLRKWLTSP VRRLSSGKAD GHVKKLAHKH 1810 1820 1830 1840 1850 1860 KKSREVRKSA DAGSQKDSDD SAATPQDETV EERGRNEGLS SGTLSKSSSS GMQSCGEEEG 1870 1880 1890 1900 1910 1920 EEGADAVPLP PPMAIQQHSL LQPDSQDDKA SSRLLVRPTS SETPSAAELV SAIEELVKSK 1930 1940 1950 1960 1970 1980 MALEDRPSSL LVDQGDSSSP SFNPSDNSLL SSSSPIDEME ERKSSSLKRR HYVLQELVET 1990 2000 2010 2020 2030 2040 ERDYVRDLGY VVEGYMALMK EDGVPDDMKG KDKIVFGNIH QIYDWHRDFF LGELEKCLED 2050 2060 2070 2080 2090 2100 PEKLGSLFVK HERRLHMYIA YCQNKPKSEH IVSEYIDTFF EDLKQRLGHR LQLTDLLIKP 2110 2120 2130 2140 2150 2160 VQRIMKYQLL LKDFLKYSKK ASLDTSELER AVEVMCIVPR RCNDMMNVGR LQGFDGKIVA 2170 2180 2190 2200 2210 2220 QGKLLLQDTF LVTDQDAGLL PRCRERRIFL FEQIVIFSEP LDKKKGFSMP GFLFKNSIKV 2230 2240 2250 2260 2270 2280 SCLCLEENVE NDPCKFALTS RTGDVVETFI LHSSSPSVRQ TWIHEINQIL ENQRNFLNAL 2290 2300 2310 2320 2330 2340 TSPIEYQRNH SGGGGGGGSG GSGGGGGSGG GGAPSGGSGH SGGPSSCGGA PSTSRSRPSR 2350 2360 2370 2380 2390 2400 IPQPVRHHPP VLVSSAASSQ AEADKMSGTS TPGPSLPPPG AAPEAGPSAP SRRPPGADAE 2410 2420 2430 2440 2450 2460 GSEREAEPIP KMKVLESPRK GAANASGSSP DAPAKDARAS LGTLPLGKPR AGAASPLNSP 2470 2480 2490 2500 2510 2520 LSSAVPSLGK EPFPPSSPLQ KGGSFWSSIP ASPASRPGSF TFPGDSDSLQ RQTPRHAAPG 2530 2540 2550 2560 KDTDRMSTCS SASEQSVQST QSNGVSASGG PRPPAPLPLS RQL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Brain.
[2]	"The DNA sequence and comparative analysis of human chromosome 5." Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S. , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M. Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-1686 (ISOFORM 4), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2715-3097 (ISOFORM 1). Tissue: Kidney and Lymph.
[5]	"The multidomain protein Trio binds the LAR transmembrane tyrosine phosphatase, contains a protein kinase domain, and has separate rac-specific and rho-specific guanine nucleotide exchange factor domains." Debant A., Serra-Pages C., Seipel K., O'Brien S., Tang M., Park S.-H., Streuli M. Proc. Natl. Acad. Sci. U.S.A. 93:5466-5471(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 38-3097 (ISOFORM 2). Tissue: Fibroblast.
[6]	Streuli M. Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 38-3097 (ISOFORM 1).
[7]	Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S., Ohara O., Nagase T., Kikuno R.F. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 362-3097 (ISOFORM 5). Tissue: Brain.
[8]	"Trio amino-terminal guanine nucleotide exchange factor domain expression promotes actin cytoskeleton reorganization, cell migration and anchorage-independent cell growth." Seipel K., Medley Q.G., Kedersha N.L., Zhang X.A., O'Brien S.P., Serra-Pages C., Hemler M.E., Streuli M. J. Cell Sci. 112:1825-1834(1999) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[9]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[10]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2282 AND SER-2455, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[11]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Leukemic T-cell.
[12]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2455 AND SER-2459, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[13]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[14]	"NMR structure and mutagenesis of the N-terminal Dbl homology domain of the nucleotide exchange factor Trio." Liu X., Wang H., Eberstadt M., Schnuchel A., Olejniczak E.T., Meadows R.P., Schkeryantz J.M., Janowick D.A., Harlan J.E., Harris E.A.S., Staunton D.E., Fesik S.W. Cell 95:269-277(1998) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR OF 1286-1466, MUTAGENESIS.
[15]	"Patterns of somatic mutation in human cancer genomes." Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R. Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANTS [LARGE SCALE ANALYSIS] THR-291; MET-1644; ARG-1690; MET-1978 AND MET-2242.
+	Additional computationally mapped references.

Web resources

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AK131423 mRNA. Translation: BAD18570.1.
AC010419 Genomic DNA. No translation available.
AC016549 Genomic DNA. No translation available.
AC016654 Genomic DNA. No translation available.
AC016656 Genomic DNA. No translation available.
AC026456 Genomic DNA. No translation available.
CH471102 Genomic DNA. Translation: EAX08047.1.
CH471102 Genomic DNA. Translation: EAX08048.1.
BC035585 mRNA. Translation: AAH35585.1.
BC017268 mRNA. Translation: AAH17268.1.
U42390 mRNA. Translation: AAC34245.1. Sequence problems.
AF091395 mRNA. Translation: AAC43042.1. Different initiation.
AB209754 mRNA. Translation: BAD92991.1.

IPI

IPI00442035.
IPI00479523.
IPI00657953.
IPI00943836.
IPI00943984.

RefSeq

NP_009049.2. NM_007118.2.

UniGene

Hs.130031.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1NTY	X-ray	1.70	A	1284-1594	[»]
2NZ8	X-ray	2.00	B	1285-1594	[»]

ProteinModelPortal

O75962.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-37578N.

IntAct

O75962. 5 interactions.

MINT

MINT-1397752.

STRING

9606.ENSP00000339299.

PTM databases

PhosphoSite

O75962.

Proteomic databases

PaxDb

O75962.

PRIDE

O75962.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000344135; ENSP00000339291; ENSG00000038382.
ENST00000344204; ENSP00000339299; ENSG00000038382.
ENST00000537187; ENSP00000446348; ENSG00000038382.

GeneID

7204.

KEGG

hsa:7204.

UCSC

uc003jff.3. human.
uc003jfh.1. human.

Organism-specific databases

CTD

7204.

GeneCards

GC05P014143.

HGNC

HGNC:12303. TRIO.

HPA

HPA008157.

MIM

601893. gene.

neXtProt

NX_O75962.

PharmGKB

PA36982.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG0515.

HOGENOM

HOG000044462.

HOVERGEN

HBG108598.

InParanoid

O75962.

KO

K08810.

OMA

IEERGRN.

OrthoDB

EOG4H462T.

PhylomeDB

O75962.

Enzyme and pathway databases

Reactome

REACT_111045. Developmental Biology.
REACT_111102. Signal Transduction.

SignaLink

O75962.

Gene expression databases

ArrayExpress

O75962.

Bgee

O75962.

CleanEx

HS_TRIO.

Genevestigator

O75962.

GermOnline

ENSG00000038382. Homo sapiens.

Family and domain databases

Gene3D

1.20.900.10. 2 hits.
2.30.29.30. 2 hits.
2.60.40.10. 1 hit.
3.40.525.10. 1 hit.

InterPro

IPR001251. CRAL-TRIO_dom.
IPR000219. DH-domain.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003598. Ig_sub2.
IPR011009. Kinase-like_dom.
IPR011993. PH_like_dom.
IPR001849. Pleckstrin_homology.
IPR000719. Prot_kinase_cat_dom.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
IPR001452. SH3_domain.
IPR018159. Spectrin/alpha-actinin.
IPR002017. Spectrin_repeat.
[Graphical view]

Pfam

PF07679. I-set. 1 hit.
PF00069. Pkinase. 1 hit.
PF00621. RhoGEF. 2 hits.
PF00018. SH3_1. 1 hit.
PF00435. Spectrin. 4 hits.
[Graphical view]

SMART

SM00408. IGc2. 1 hit.
SM00233. PH. 2 hits.
SM00325. RhoGEF. 2 hits.
SM00220. S_TKc. 1 hit.
SM00516. SEC14. 1 hit.
SM00326. SH3. 2 hits.
SM00150. SPEC. 6 hits.
[Graphical view]

SUPFAM

SSF52087. CRAL_TRIO_C. 1 hit.
SSF48065. DH-domain. 2 hits.
SSF56112. Kinase_like. 1 hit.
SSF50044. SH3. 2 hits.

PROSITE

PS50191. CRAL_TRIO. 1 hit.
PS00741. DH_1. False negative.
PS50010. DH_2. 2 hits.
PS50835. IG_LIKE. 1 hit.
PS50003. PH_DOMAIN. 2 hits.
PS00107. PROTEIN_KINASE_ATP. False negative.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

TRIO. human.

EvolutionaryTrace

O75962.

GenomeRNAi

7204.

NextBio

28234.

SOURCE

Search...

Entry information

Entry name

TRIO_HUMAN

Accession

Primary (citable) accession number: O75962
Secondary accession number(s): D3DTD1 , Q13458, Q59EQ7, Q6PJC9, Q6ZN05, Q8IWK8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	September 1, 2009
Last modified:	May 29, 2013
This is version 140 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.