Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

O75955 (FLOT1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 128. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Flotillin-1
Gene names
Name:FLOT1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length427 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles.

Subunit structure

Heterooligomeric complex of flotillin-1 and flotillin-2 and caveolin-1 and caveolin-2 By similarity. Interacts with ECM29. Ref.10

Subcellular location

Cell membrane; Peripheral membrane protein. Membranecaveola; Peripheral membrane protein. Melanosome. Endosome. Note: Membrane-associated protein of caveolae. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Ref.6 Ref.10

Sequence similarities

Belongs to the band 7/mec-2 family. Flotillin subfamily.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 427427Flotillin-1
PRO_0000094044

Amino acid modifications

Modified residue191Phosphoserine Ref.7
Modified residue3851Phosphoserine Ref.9

Natural variations

Natural variant521S → N.
Corresponds to variant rs3180825 [ dbSNP | Ensembl ].
VAR_048415

Experimental info

Sequence conflict1141H → N in AAD40192. Ref.2
Sequence conflict1141H → N in AAF17215. Ref.5
Sequence conflict250 – 2534EEQR → DFSQ in AAF17215. Ref.5

Sequences

Sequence LengthMass (Da)Tools
O75955 [UniParc].

Last modified November 28, 2002. Version 3.
Checksum: C69E5421E565F53A

FASTA42747,355
        10         20         30         40         50         60 
MFFTCGPNEA MVVSGFCRSP PVMVAGGRVF VLPCIQQIQR ISLNTLTLNV KSEKVYTRHG 

        70         80         90        100        110        120 
VPISVTGIAQ VKIQGQNKEM LAAACQMFLG KTEAEIAHIA LETLEGHQRA IMAHMTVEEI 

       130        140        150        160        170        180 
YKDRQKFSEQ VFKVASSDLV NMGISVVSYT LKDIHDDQDY LHSLGKARTA QVQKDARIGE 

       190        200        210        220        230        240 
AEAKRDAGIR EAKAKQEKVS AQYLSEIEMA KAQRDYELKK AAYDIEVNTR RAQADLAYQL 

       250        260        270        280        290        300 
QVAKTKQQIE EQRVQVQVVE RAQQVAVQEQ EIARREKELE ARVRKPAEAE RYKLERLAEA 

       310        320        330        340        350        360 
EKSQLIMQAE AEAASVRMRG EAEAFAIGAR ARAEAEQMAK KAEAFQLYQE AAQLDMLLEK 

       370        380        390        400        410        420 
LPQVAEEISG PLTSANKITL VSSGSGTMGA AKVTGEVLDI LTRLPESVER LTGVSISQVN 


HKPLRTA 

« Hide

References

« Hide 'large scale' references
[1]"Flotillin-1: gene structure: cDNA cloning from human lung and the identification of alternative polyadenylation signals."
Edgar A.J., Polak J.M.
Int. J. Biochem. Cell Biol. 33:53-64(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Lung.
[2]"Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells."
Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G., Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W., Tao J., Huang Q.-H., Zhou J., Hu G.-X. expand/collapse author list , Gu J., Chen S.-J., Chen Z.
Genome Res. 10:1546-1560(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Umbilical cord blood.
[3]"Homo sapiens 2,229,817bp genomic DNA of 6p21.3 HLA class I region."
Shiina S., Tamiya G., Oka A., Inoko H.
Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Lung.
[5]"Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning."
Hu R.-M., Han Z.-G., Song H.-D., Peng Y.-D., Huang Q.-H., Ren S.-X., Gu Y.-J., Huang C.-H., Li Y.-B., Jiang C.-L., Fu G., Zhang Q.-H., Gu B.-W., Dai M., Mao Y.-F., Gao G.-F., Rong R., Ye M. expand/collapse author list , Zhou J., Xu S.-H., Gu J., Shi J.-X., Jin W.-R., Zhang C.-K., Wu T.-M., Huang G.-Y., Chen Z., Chen M.-D., Chen J.-L.
Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-253.
Tissue: Adrenal gland.
[6]"Proteomic and bioinformatic characterization of the biogenesis and function of melanosomes."
Chi A., Valencia J.C., Hu Z.-Z., Watabe H., Yamaguchi H., Mangini N.J., Huang H., Canfield V.A., Cheng K.C., Yang F., Abe R., Yamagishi S., Shabanowitz J., Hearing V.J., Wu C., Appella E., Hunt D.F.
J. Proteome Res. 5:3135-3144(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
Tissue: Melanoma.
[7]"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-19, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[8]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[9]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-385, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
[10]"A protein interaction network for Ecm29 links the 26 S proteasome to molecular motors and endosomal components."
Gorbea C., Pratt G., Ustrell V., Bell R., Sahasrabudhe S., Hughes R.E., Rechsteiner M.
J. Biol. Chem. 285:31616-31633(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, INTERACTION WITH ECM29.
[11]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF089750 mRNA. Translation: AAC35387.1.
AF085357 mRNA. Translation: AAD40192.1.
BA000025 Genomic DNA. Translation: BAB63320.1.
BC001146 mRNA. Translation: AAH01146.1.
AF117234 mRNA. Translation: AAF17215.1.
RefSeqNP_005794.1. NM_005803.2.
XP_005248837.1. XM_005248780.1.
XP_005272816.1. XM_005272759.1.
XP_005274966.1. XM_005274909.1.
XP_005275392.1. XM_005275335.1.
XP_005275559.1. XM_005275502.1.
UniGeneHs.179986.

3D structure databases

ProteinModelPortalO75955.
SMRO75955. Positions 39-170.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid115506. 69 interactions.
IntActO75955. 19 interactions.
MINTMINT-3002104.
STRING9606.ENSP00000372873.

PTM databases

PhosphoSiteO75955.

Proteomic databases

PaxDbO75955.
PRIDEO75955.

Protocols and materials databases

DNASU10211.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000376389; ENSP00000365569; ENSG00000137312.
ENST00000383382; ENSP00000372873; ENSG00000206379.
ENST00000383562; ENSP00000373056; ENSG00000206480.
ENST00000436822; ENSP00000391438; ENSG00000232280.
ENST00000444632; ENSP00000388861; ENSG00000230143.
GeneID10211.
KEGGhsa:10211.
UCSCuc003nrm.3. human.

Organism-specific databases

CTD10211.
GeneCardsGC06M030695.
GC06Mj30685.
GC06Mk30685.
GC06Mn30685.
GC06Mo30686.
H-InvDBHIX0166980.
HGNCHGNC:3757. FLOT1.
HPACAB007766.
HPA001393.
MIM606998. gene.
neXtProtNX_O75955.
PharmGKBPA28175.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG2268.
HOGENOMHOG000240804.
HOVERGENHBG051628.
InParanoidO75955.
KOK07192.
OMAKDVRDKN.
PhylomeDBO75955.
TreeFamTF324879.

Gene expression databases

ArrayExpressO75955.
BgeeO75955.
CleanExHS_FLOT1.
GenevestigatorO75955.

Family and domain databases

InterProIPR001107. Band_7.
IPR027705. Flotillin_fam.
[Graphical view]
PANTHERPTHR13806. PTHR13806. 1 hit.
PfamPF01145. Band_7. 1 hit.
[Graphical view]
SMARTSM00244. PHB. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSFLOT1. human.
GeneWikiFLOT1.
GenomeRNAi10211.
NextBio38660.
PROO75955.
SOURCESearch...

Entry information

Entry nameFLOT1_HUMAN
AccessionPrimary (citable) accession number: O75955
Secondary accession number(s): Q969J8, Q9UHW1, Q9UNV8
Entry history
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: November 28, 2002
Last modified: March 19, 2014
This is version 128 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 6

Human chromosome 6: entries, gene names and cross-references to MIM