O75955 (FLOT1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Flotillin-1

Gene names

Name:

FLOT1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	427 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles.
Subunit structure	Heterooligomeric complex of flotillin-1 and flotillin-2 and caveolin-1 and caveolin-2 By similarity. Interacts with ECM29. Ref.12
Subcellular location	Cell membrane; Peripheral membrane protein. Membrane › caveola; Peripheral membrane protein. Melanosome. Endosome. Note: Membrane-associated protein of caveolae. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Ref.6 Ref.12
Sequence similarities	Belongs to the band 7/mec-2 family. Flotillin subfamily.

Ontologies

Keywords
Cellular component	Cell membrane Endosome Membrane
Coding sequence diversity	Polymorphism
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	centriolar satellite Inferred from direct assay. Source: BHF-UCL endosome Inferred from direct assay Ref.12. Source: UniProtKB integral to membrane Traceable author statement. Source: ProtInc melanosome Inferred from electronic annotation. Source: UniProtKB-SubCell membrane fraction Inferred from direct assay. Source: BHF-UCL
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
APP	P05067	5	EBI-603643,EBI-77613

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 427	427	Flotillin-1		PRO_0000094044
Amino acid modifications
	Modified residue	19	1	Phosphoserine Ref.8 Ref.9
	Modified residue	203	1	Phosphotyrosine Ref.7
	Modified residue	385	1	Phosphoserine Ref.11
	Modified residue	387	1	Phosphothreonine Ref.10
Natural variations
	Natural variant	52	1	S → N. Corresponds to variant rs3180825 [ dbSNP | Ensembl ].		VAR_048415
Experimental info
	Sequence conflict	114	1	H → N in AAD40192. Ref.2
	Sequence conflict	114	1	H → N in AAF17215. Ref.5
	Sequence conflict	250 – 253	4	EEQR → DFSQ in AAF17215. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	O75955 [UniParc]. Last modified November 28, 2002. Version 3. Checksum: C69E5421E565F53A	FASTA	427	47,355
	10 20 30 40 50 60 MFFTCGPNEA MVVSGFCRSP PVMVAGGRVF VLPCIQQIQR ISLNTLTLNV KSEKVYTRHG 70 80 90 100 110 120 VPISVTGIAQ VKIQGQNKEM LAAACQMFLG KTEAEIAHIA LETLEGHQRA IMAHMTVEEI 130 140 150 160 170 180 YKDRQKFSEQ VFKVASSDLV NMGISVVSYT LKDIHDDQDY LHSLGKARTA QVQKDARIGE 190 200 210 220 230 240 AEAKRDAGIR EAKAKQEKVS AQYLSEIEMA KAQRDYELKK AAYDIEVNTR RAQADLAYQL 250 260 270 280 290 300 QVAKTKQQIE EQRVQVQVVE RAQQVAVQEQ EIARREKELE ARVRKPAEAE RYKLERLAEA 310 320 330 340 350 360 EKSQLIMQAE AEAASVRMRG EAEAFAIGAR ARAEAEQMAK KAEAFQLYQE AAQLDMLLEK 370 380 390 400 410 420 LPQVAEEISG PLTSANKITL VSSGSGTMGA AKVTGEVLDI LTRLPESVER LTGVSISQVN HKPLRTA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Flotillin-1: gene structure: cDNA cloning from human lung and the identification of alternative polyadenylation signals." Edgar A.J., Polak J.M. Int. J. Biochem. Cell Biol. 33:53-64(2001) [PubMed: 11167132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Lung.
[2]	"Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells." Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G., Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W., Tao J., Huang Q.-H., Zhou J., Hu G.-X. , Gu J., Chen S.-J., Chen Z. Genome Res. 10:1546-1560(2000) [PubMed: 11042152] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Umbilical cord blood.
[3]	"Homo sapiens 2,229,817bp genomic DNA of 6p21.3 HLA class I region." Shiina S., Tamiya G., Oka A., Inoko H. Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
[5]	"Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning." Hu R.-M., Han Z.-G., Song H.-D., Peng Y.-D., Huang Q.-H., Ren S.-X., Gu Y.-J., Huang C.-H., Li Y.-B., Jiang C.-L., Fu G., Zhang Q.-H., Gu B.-W., Dai M., Mao Y.-F., Gao G.-F., Rong R., Ye M. , Zhou J., Xu S.-H., Gu J., Shi J.-X., Jin W.-R., Zhang C.-K., Wu T.-M., Huang G.-Y., Chen Z., Chen M.-D., Chen J.-L. Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000) [PubMed: 10931946] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-253. Tissue: Adrenal gland.
[6]	"Proteomic and bioinformatic characterization of the biogenesis and function of melanosomes." Chi A., Valencia J.C., Hu Z.-Z., Watabe H., Yamaguchi H., Mangini N.J., Huang H., Canfield V.A., Cheng K.C., Yang F., Abe R., Yamagishi S., Shabanowitz J., Hearing V.J., Wu C., Appella E., Hunt D.F. J. Proteome Res. 5:3135-3144(2006) [PubMed: 17081065] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. Tissue: Melanoma.
[7]	"Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer." Rikova K., Guo A., Zeng Q., Possemato A., Yu J., Haack H., Nardone J., Lee K., Reeves C., Li Y., Hu Y., Tan Z., Stokes M., Sullivan L., Mitchell J., Wetzel R., Macneill J., Ren J.M. , Yuan J., Bakalarski C.E., Villen J., Kornhauser J.M., Smith B., Li D., Zhou X., Gygi S.P., Gu T.-L., Polakiewicz R.D., Rush J., Comb M.J. Cell 131:1190-1203(2007) [PubMed: 18083107] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-203, MASS SPECTROMETRY. Tissue: Lung carcinoma.
[8]	"Evaluation of the low-specificity protease elastase for large-scale phosphoproteome analysis." Wang B., Malik R., Nigg E.A., Korner R. Anal. Chem. 80:9526-9533(2008) [PubMed: 19007248] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-19, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[9]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-19, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[10]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-387, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[11]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-385, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[12]	"A protein interaction network for Ecm29 links the 26 S proteasome to molecular motors and endosomal components." Gorbea C., Pratt G., Ustrell V., Bell R., Sahasrabudhe S., Hughes R.E., Rechsteiner M. J. Biol. Chem. 285:31616-31633(2010) [PubMed: 20682791] [Abstract] Cited for: SUBCELLULAR LOCATION, INTERACTION WITH ECM29.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF089750 mRNA. Translation: AAC35387.1. AF085357 mRNA. Translation: AAD40192.1. BA000025 Genomic DNA. Translation: BAB63320.1. BC001146 mRNA. Translation: AAH01146.1. AF117234 mRNA. Translation: AAF17215.1.
IPI	IPI00027438.
RefSeq	NP_005794.1. NM_005803.2.
UniGene	Hs.179986.
3D structure databases
ProteinModelPortal	O75955.
SMR	O75955. Positions 36-173.
ModBase	Search...
Protein-protein interaction databases
IntAct	O75955. 13 interactions.
MINT	MINT-3002104.
STRING	O75955.
PTM databases
PhosphoSite	O75955.
Proteomic databases
PRIDE	O75955.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000376389; ENSP00000365569; ENSG00000137312. ENST00000383382; ENSP00000372873; ENSG00000206379. ENST00000383562; ENSP00000373056; ENSG00000206480. ENST00000436822; ENSP00000391438; ENSG00000232280. ENST00000444632; ENSP00000388861; ENSG00000230143.
GeneID	10211.
KEGG	hsa:10211.
UCSC	uc003nrm.1. human.
Organism-specific databases
CTD	10211.
GeneCards	GC06M030695.
HGNC	HGNC:3757. FLOT1.
HPA	CAB007766. HPA001393.
MIM	606998. gene.
neXtProt	NX_O75955.
PharmGKB	PA28175.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	HBG729052.
HOVERGEN	HBG051628.
InParanoid	O75955.
OMA	QRAIMGS.
OrthoDB	EOG473PRK.
PhylomeDB	O75955.
Gene expression databases
CleanEx	HS_FLOT1.
Genevestigator	O75955.
GermOnline	ENSG00000137312. Homo sapiens.
Family and domain databases
InterPro	IPR001107. Band_7. [Graphical view]
KO	K07192.
Pfam	PF01145. Band_7. 1 hit. [Graphical view]
SMART	SM00244. PHB. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	38660.
SOURCE	Search...

Entry information

Entry name

FLOT1_HUMAN

Accession

Primary (citable) accession number: O75955
Secondary accession number(s): Q969J8, Q9UHW1, Q9UNV8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	November 28, 2002
Last modified:	January 25, 2012
This is version 106 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.