ID   TSN9_HUMAN              Reviewed;         239 AA.
AC   O75954; D3DUQ7; Q53FV2; Q6FGJ8;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1998, sequence version 1.
DT   27-MAR-2024, entry version 161.
DE   RecName: Full=Tetraspanin-9;
DE            Short=Tspan-9;
DE   AltName: Full=Tetraspan NET-5;
GN   Name=TSPAN9; Synonyms=NET5;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=10719184; DOI=10.1016/s0167-4838(00)00022-4;
RA   Serru V., Dessen P., Boucheix C., Rubinstein E.;
RT   "Sequence and expression of seven new tetraspans.";
RL   Biochim. Biophys. Acta 1478:159-163(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA   Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.;
RT   "Cloning of human full open reading frames in Gateway(TM) system entry
RT   vector (pDONR201).";
RL   Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Pancreas;
RA   Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y.,
RA   Tanaka A., Yokoyama S.;
RL   Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16541075; DOI=10.1038/nature04569;
RA   Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y.,
RA   Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C.,
RA   Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C.,
RA   Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R.,
RA   Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E.,
RA   Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y.,
RA   Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G.,
RA   Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H.,
RA   Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S.,
RA   Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M.,
RA   Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H.,
RA   Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q.,
RA   Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V.,
RA   Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E.,
RA   Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K.,
RA   Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D.,
RA   Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R.,
RA   David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E.,
RA   D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N.,
RA   Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N.,
RA   Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R.,
RA   Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S.,
RA   LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H.,
RA   Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P.,
RA   Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G.,
RA   Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E.,
RA   Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S.,
RA   Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O.,
RA   Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J.,
RA   Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A.,
RA   Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M.,
RA   Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I.,
RA   Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A.,
RA   Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y.,
RA   Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A.,
RA   Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F.,
RA   Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L.,
RA   Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G.,
RA   Gibbs R.A.;
RT   "The finished DNA sequence of human chromosome 12.";
RL   Nature 440:346-351(2006).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA   Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA   Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA   Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA   Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA   Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA   Hunkapiller M.W., Myers E.W., Venter J.C.;
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN   [7]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Salivary gland;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [8]
RP   TISSUE SPECIFICITY.
RX   PubMed=18795891; DOI=10.1042/bj20081126;
RA   Protty M.B., Watkins N.A., Colombo D., Thomas S.G., Heath V.L.,
RA   Herbert J.M., Bicknell R., Senis Y.A., Ashman L.K., Berditchevski F.,
RA   Ouwehand W.H., Watson S.P., Tomlinson M.G.;
RT   "Identification of Tspan9 as a novel platelet tetraspanin and the collagen
RT   receptor GPVI as a component of tetraspanin microdomains.";
RL   Biochem. J. 417:391-400(2009).
CC   -!- SUBUNIT: Found in a complex with GP6. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250}; Multi-pass membrane
CC       protein {ECO:0000250}. Note=Colocalizes with GP6 in tetraspanin
CC       microdomains on the platelet surface. {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Expressed in megakaryocytes and platelets (at
CC       protein level). {ECO:0000269|PubMed:18795891}.
CC   -!- PTM: Glycosylated. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the tetraspanin (TM4SF) family. {ECO:0000305}.
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DR   EMBL; AF089749; AAC35859.1; -; mRNA.
DR   EMBL; AK027366; BAG51307.1; -; mRNA.
DR   EMBL; CR542109; CAG46906.1; -; mRNA.
DR   EMBL; AK223179; BAD96899.1; -; mRNA.
DR   EMBL; AC005912; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CH471116; EAW88868.1; -; Genomic_DNA.
DR   EMBL; CH471116; EAW88869.1; -; Genomic_DNA.
DR   EMBL; BC071881; AAH71881.1; -; mRNA.
DR   CCDS; CCDS8520.1; -.
DR   RefSeq; NP_001161792.1; NM_001168320.1.
DR   RefSeq; NP_006666.1; NM_006675.4.
DR   AlphaFoldDB; O75954; -.
DR   SMR; O75954; -.
DR   BioGRID; 116076; 9.
DR   IntAct; O75954; 4.
DR   MINT; O75954; -.
DR   STRING; 9606.ENSP00000011898; -.
DR   TCDB; 8.A.40.1.22; the tetraspanin (tetraspanin) family.
DR   GlyCosmos; O75954; 1 site, No reported glycans.
DR   GlyGen; O75954; 1 site.
DR   iPTMnet; O75954; -.
DR   PhosphoSitePlus; O75954; -.
DR   SwissPalm; O75954; -.
DR   BioMuta; TSPAN9; -.
DR   EPD; O75954; -.
DR   jPOST; O75954; -.
DR   MassIVE; O75954; -.
DR   MaxQB; O75954; -.
DR   PaxDb; 9606-ENSP00000011898; -.
DR   PeptideAtlas; O75954; -.
DR   ProteomicsDB; 50320; -.
DR   Antibodypedia; 2820; 189 antibodies from 32 providers.
DR   DNASU; 10867; -.
DR   Ensembl; ENST00000011898.10; ENSP00000011898.5; ENSG00000011105.14.
DR   Ensembl; ENST00000537971.5; ENSP00000444799.1; ENSG00000011105.14.
DR   GeneID; 10867; -.
DR   KEGG; hsa:10867; -.
DR   MANE-Select; ENST00000011898.10; ENSP00000011898.5; NM_006675.5; NP_006666.1.
DR   UCSC; uc001qlp.4; human.
DR   AGR; HGNC:21640; -.
DR   CTD; 10867; -.
DR   DisGeNET; 10867; -.
DR   GeneCards; TSPAN9; -.
DR   HGNC; HGNC:21640; TSPAN9.
DR   HPA; ENSG00000011105; Tissue enhanced (brain, heart muscle).
DR   MIM; 613137; gene.
DR   neXtProt; NX_O75954; -.
DR   OpenTargets; ENSG00000011105; -.
DR   PharmGKB; PA142670694; -.
DR   VEuPathDB; HostDB:ENSG00000011105; -.
DR   eggNOG; KOG3882; Eukaryota.
DR   GeneTree; ENSGT00940000158225; -.
DR   HOGENOM; CLU_055524_4_3_1; -.
DR   InParanoid; O75954; -.
DR   OMA; DTIFMCI; -.
DR   OrthoDB; 3234802at2759; -.
DR   PhylomeDB; O75954; -.
DR   TreeFam; TF352892; -.
DR   PathwayCommons; O75954; -.
DR   SignaLink; O75954; -.
DR   BioGRID-ORCS; 10867; 8 hits in 1154 CRISPR screens.
DR   ChiTaRS; TSPAN9; human.
DR   GenomeRNAi; 10867; -.
DR   Pharos; O75954; Tbio.
DR   PRO; PR:O75954; -.
DR   Proteomes; UP000005640; Chromosome 12.
DR   RNAct; O75954; Protein.
DR   Bgee; ENSG00000011105; Expressed in apex of heart and 194 other cell types or tissues.
DR   ExpressionAtlas; O75954; baseline and differential.
DR   GO; GO:0005925; C:focal adhesion; HDA:UniProtKB.
DR   GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
DR   GO; GO:0097197; C:tetraspanin-enriched microdomain; IDA:UniProtKB.
DR   CDD; cd03165; NET-5_like_LEL; 1.
DR   Gene3D; 1.10.1450.10; Tetraspanin; 1.
DR   InterPro; IPR018499; Tetraspanin/Peripherin.
DR   InterPro; IPR000301; Tetraspanin_animals.
DR   InterPro; IPR018503; Tetraspanin_CS.
DR   InterPro; IPR008952; Tetraspanin_EC2_sf.
DR   PANTHER; PTHR19282; TETRASPANIN; 1.
DR   PANTHER; PTHR19282:SF41; TETRASPANIN-9; 1.
DR   Pfam; PF00335; Tetraspanin; 1.
DR   PIRSF; PIRSF002419; Tetraspanin; 1.
DR   PRINTS; PR00259; TMFOUR.
DR   SUPFAM; SSF48652; Tetraspanin; 1.
DR   PROSITE; PS00421; TM4_1; 1.
DR   Genevisible; O75954; HS.
PE   1: Evidence at protein level;
KW   Glycoprotein; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..239
FT                   /note="Tetraspanin-9"
FT                   /id="PRO_0000219253"
FT   TOPO_DOM        1..13
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        14..34
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        35..55
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        56..76
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        77..85
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        86..106
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        107..203
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        204..224
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        225..239
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        180
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        216
FT                   /note="Q -> R (in Ref. 4; BAD96899)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   239 AA;  26779 MW;  DD7BA332BF6584EB CRC64;
     MARGCLCCLK YMMFLFNLIF WLCGCGLLGV GIWLSVSQGN FATFSPSFPS LSAANLVIAI
     GTIVMVTGFL GCLGAIKENK CLLLSFFIVL LVILLAELIL LILFFVYMDK VNENAKKDLK
     EGLLLYHTEN NVGLKNAWNI IQAEMRCCGV TDYTDWYPVL GENTVPDRCC MENSQGCGRN
     ATTPLWRTGC YEKVKMWFDD NKHVLGTVGM CILIMQILGM AFSMTLFQHI HRTGKKYDA
//