Reviewed, UniProtKB/Swiss-Prot O75953 (DNJB5_HUMAN)

Names and origin

Protein names

Recommended name:
    DnaJ homolog subfamily B member 5
Alternative name(s):
    Heat shock protein Hsp40-3
    Heat shock protein cognate 40
      Short name=Hsc40
      Short name=Hsp40-2

Gene names

Name:	DNAJB5
Synonyms:	HSC40

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	348 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Induction	Expressed under normal conditions, its expression can further be increased after various stress treatments.
Sequence similarities	Contains 1 J domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Molecular function	Chaperone
Gene Ontology (GO)
Biological process	protein folding Inferred from electronic annotation. Source: InterPro response to unfolded protein Ref.1 Inferred from expression pattern. Source: UniProtKB
Molecular function	heat shock protein binding Inferred from electronic annotation. Source: InterPro unfolded protein binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O75953-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O75953-2)

The sequence of this isoform differs from the canonical sequence as follows:
     344-348: HLPCS → QYPQHSLHSMCTQLDVHWTLATFSKMQKKATGFQENVPVPDPF

Notes: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 348	348	DnaJ homolog subfamily B member 5		PRO_0000071023
Regions
	Domain	4 – 68	65	J
Natural variations
	Alternative sequence	344 – 348	5	HLPCS → QYPQHSLHSMCTQLDVHWTL ATFSKMQKKATGFQENVPVP DPF in isoform 2.		VSP_030167
Experimental info
	Sequence conflict	190 – 196	7	MKITRRR → IEDHKAS in AAM10498. Ref.2
	Sequence conflict	234 – 235	2	TP → HL in AAM10498. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: DC9FE45DE4FD8CFC	FASTA	348	39,133
	10 20 30 40 50 60 MGKDYYKILG IPSGANEDEI KKAYRKMALK YHPDKNKEPN AEEKFKEIAE AYDVLSDPKK 70 80 90 100 110 120 RGLYDQYGEE GLKTGGGTSG GSSGSFHYTF HGDPHATFAS FFGGSNPFDI FFASSRSTRP 130 140 150 160 170 180 FSGFDPDDMD VDEDEDPFGA FGRFGFNGLS RGPRRAPEPL YPRRKVQDPP VVHELRVSLE 190 200 210 220 230 240 EIYHGSTKRM KITRRRLNPD GRTVRTEDKI LHIVIKRGWK EGTKITFPKE GDATPDNIPA 250 260 270 280 290 300 DIVFVLKDKP HAHFRRDGTN VLYSALISLK EALCGCTVNI PTIDGRVIPL PCNDVIKPGT 310 320 330 340 VKRLRGEGLP FPKVPTQRGD LIVEFKVRFP DRLTPQTRQI LKQHLPCS
	Isoform 2 [UniParc]. Checksum: 9A91CF7A0AC84D83		386	43,552
	10 20 30 40 50 60 MGKDYYKILG IPSGANEDEI KKAYRKMALK YHPDKNKEPN AEEKFKEIAE AYDVLSDPKK 70 80 90 100 110 120 RGLYDQYGEE GLKTGGGTSG GSSGSFHYTF HGDPHATFAS FFGGSNPFDI FFASSRSTRP 130 140 150 160 170 180 FSGFDPDDMD VDEDEDPFGA FGRFGFNGLS RGPRRAPEPL YPRRKVQDPP VVHELRVSLE 190 200 210 220 230 240 EIYHGSTKRM KITRRRLNPD GRTVRTEDKI LHIVIKRGWK EGTKITFPKE GDATPDNIPA 250 260 270 280 290 300 DIVFVLKDKP HAHFRRDGTN VLYSALISLK EALCGCTVNI PTIDGRVIPL PCNDVIKPGT 310 320 330 340 350 360 VKRLRGEGLP FPKVPTQRGD LIVEFKVRFP DRLTPQTRQI LKQQYPQHSL HSMCTQLDVH 370 380 WTLATFSKMQ KKATGFQENV PVPDPF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Hsc40, a new member of the hsp40 family, exhibits similar expression profile to that of hsc70 in mammalian cells." Chen M.-S., Roti J.R., Laszlo A. Gene 238:333-341(1999) [PubMed: 10570961] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Colon.
[2]	"Cloning and expression of a new human cDNA homology to human heat-shock protein 40 mRNA." Fu Q., Yu L., Yue P., Zhou Y., Jiang J.X., Zhao S.Y. Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]	"DNA sequence and analysis of human chromosome 9." Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I. Nature 429:369-374(2004) [PubMed: 15164053] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Skin.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF088982 mRNA. Translation: AAC35860.1. AF087870 mRNA. Translation: AAM10498.1. AL355377 Genomic DNA. Translation: CAI13810.1. CH471071 Genomic DNA. Translation: EAW58407.1. BC012115 mRNA. Translation: AAH12115.1.
RefSeq	NP_036398.3.
UniGene	Hs.237506
3D structure databases
HSSP	HSSP built from PDB template 1HDJ based on UniProtKB P25685.
SMR	O75953. Positions 1-76.
ModBase	Search...
PTM databases
PhosphoSite	O75953.
Genome annotation databases
Ensembl	ENSG00000137094. Homo sapiens. [Contig view]
GeneID	25822.
KEGG	hsa:25822.
Organism-specific databases
H-InvDB	HIX0008007.
HGNC	HGNC:14887. DNAJB5.
MIM	611328. gene.
PharmGKB	PA27417.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOVERGEN	O75953.
Gene expression databases
ArrayExpress	O75953.
CleanEx	HS_DNAJB5.
GermOnline	ENSG00000137094. Homo sapiens.
Family and domain databases
InterPro	IPR002939. DnaJ_C. IPR001623. DnaJ_N. IPR015609. Hsp40/DnaJ_Rel. IPR003095. Hsp_DnaJ. [Graphical view]
Gene3D	G3DSA:1.10.287.110. DnaJ_N. 1 hit.
PANTHER	PTHR11821. Hsp40/DnaJ_Rel. 1 hit.
Pfam	PF00226. DnaJ. 1 hit. PF01556. DnaJ_C. 1 hit. [Graphical view]
PRINTS	PR00625. DNAJPROTEIN.
SMART	SM00271. DnaJ. 1 hit. [Graphical view]
PROSITE	PS00636. DNAJ_1. 1 hit. PS50076. DNAJ_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	47081.
SOURCE	Search...

Entry information

Entry name

DNJB5_HUMAN

Accession

Primary (citable) accession number: O75953
Secondary accession number(s): Q5T656, Q8TDR7, Q96EM4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 18, 2001
Last sequence update:	November 1, 1998
Last modified:	November 25, 2008
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)