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Protein

Dynactin subunit 3

Gene

DCTN3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Together with dynein may be involved in spindle assembly and cytokinesis.1 Publication

GO - Molecular functioni

  • structural molecule activity Source: ProtInc

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiR-HSA-2132295. MHC class II antigen presentation.
R-HSA-2565942. Regulation of PLK1 Activity at G2/M Transition.
R-HSA-380259. Loss of Nlp from mitotic centrosomes.
R-HSA-380270. Recruitment of mitotic centrosome proteins and complexes.
R-HSA-5620912. Anchoring of the basal body to the plasma membrane.
R-HSA-6807878. COPI-mediated anterograde transport.
R-HSA-6811436. COPI-independent Golgi-to-ER retrograde traffic.
R-HSA-8854518. AURKA Activation by TPX2.

Names & Taxonomyi

Protein namesi
Recommended name:
Dynactin subunit 3
Alternative name(s):
Dynactin complex subunit 22 kDa subunit
Short name:
p22
Gene namesi
Name:DCTN3Imported
Synonyms:DCTN22
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 9

Organism-specific databases

HGNCiHGNC:2713. DCTN3.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Cytoplasmcytoskeletonmicrotubule organizing centercentrosome 1 Publication
  • Chromosomecentromerekinetochore 1 Publication
  • Cytoplasmcytoskeletonspindle 1 Publication
  • Cleavage furrow 1 Publication
  • Midbody 1 Publication

  • Note: Localizes to punctate cytoplasmic structures and to the centrosome during interphase, and to kinetochores and to spindle poles throughout mitosis. Colocalizes with dynein to the cleavage furrow and to midbody of dividing cells.

GO - Cellular componenti

  • centrosome Source: UniProtKB
  • cleavage furrow Source: UniProtKB-SubCell
  • condensed chromosome kinetochore Source: UniProtKB-SubCell
  • cytosol Source: Reactome
  • dynactin complex Source: UniProtKB
  • midbody Source: UniProtKB-SubCell
  • perinuclear region of cytoplasm Source: UniProtKB
  • spindle Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore

Pathology & Biotechi

Organism-specific databases

DisGeNETi11258.
OpenTargetsiENSG00000137100.
PharmGKBiPA27182.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002256032 – 186Dynactin subunit 3Add BLAST185

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiO75935.
MaxQBiO75935.
PaxDbiO75935.
PeptideAtlasiO75935.
PRIDEiO75935.
TopDownProteomicsiO75935-1. [O75935-1]
O75935-3. [O75935-3]

PTM databases

iPTMnetiO75935.
PhosphoSitePlusiO75935.

Expressioni

Tissue specificityi

Ubiquitously expressed. Highly expressed in muscle and pancreas and detected at lower levels in brain.1 Publication

Gene expression databases

BgeeiENSG00000137100.
CleanExiHS_DCTN3.
ExpressionAtlasiO75935. baseline and differential.
GenevisibleiO75935. HS.

Organism-specific databases

HPAiHPA044905.

Interactioni

Subunit structurei

Subunit of dynactin, a multiprotein complex associated with dynein.

Protein-protein interaction databases

BioGridi116418. 47 interactors.
IntActiO75935. 21 interactors.
MINTiMINT-1033946.
STRINGi9606.ENSP00000259632.

Structurei

3D structure databases

ProteinModelPortaliO75935.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili135 – 157Sequence analysisAdd BLAST23

Sequence similaritiesi

Belongs to the dynactin subunit 3 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IXN7. Eukaryota.
ENOG410Y8FP. LUCA.
GeneTreeiENSGT00390000016210.
HOGENOMiHOG000231666.
HOVERGENiHBG053181.
InParanoidiO75935.
KOiK10425.
OMAiYNKMMFL.
OrthoDBiEOG091G0MGW.
PhylomeDBiO75935.
TreeFamiTF105248.

Family and domain databases

InterProiIPR009991. Dynactin_p22.
[Graphical view]
PfamiPF07426. Dynactin_p22. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 11 Publication (identifier: O75935-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGLTDLQRL QARVEELERW VYGPGGARGS RKVADGLVKV QVALGNISSK
60 70 80 90 100
RERVKILYKK IEDLIKYLDP EYIDRIAIPD ASKLQFILAE EQFILSQVAL
110 120 130 140 150
LEQVNALVPM LDSAHIKAVP EHAARLQRLA QIHIQQQDQC VEITEESKAL
160 170 180
LEEYNKTTML LSKQFVQWDE LLCQLEAATQ VKPAEE
Length:186
Mass (Da):21,119
Last modified:November 1, 1998 - v1
Checksum:i70A0B26E24603A77
GO
Isoform 21 Publication (identifier: O75935-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     138-186: DQCVEITEES...AATQVKPAEE → APWGVGVRDE...GPTGPVCGNH

Note: No experimental confirmation available.Curated
Show »
Length:176
Mass (Da):19,469
Checksum:iFE50412C172E1922
GO
Isoform 3Curated (identifier: O75935-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     90-117: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:158
Mass (Da):18,001
Checksum:i218ABF3564911AB1
GO

Sequence cautioni

The sequence CAI13144 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAI14178 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAI14180 differs from that shown. Reason: Erroneous initiation.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_05196190 – 117Missing in isoform 3. CuratedAdd BLAST28
Alternative sequenceiVSP_051964138 – 186DQCVE…KPAEE → APWGVGVRDEAGSLVEDVGF AQFLSVLHFGPTGPVCGNH in isoform 2. 2 PublicationsAdd BLAST49

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF082513 mRNA. Translation: AAC61280.1.
CR541889 mRNA. Translation: CAG46687.1.
AK314730 mRNA. Translation: BAG37272.1.
AL160270, AL450283 Genomic DNA. Translation: CAI13143.1.
AL160270, AL450283 Genomic DNA. Translation: CAI13144.1. Different initiation.
AL160270, AL450283 Genomic DNA. Translation: CAI13145.1.
AL450283 Genomic DNA. Translation: CAI14178.1. Sequence problems.
AL450283, AL160270 Genomic DNA. Translation: CAI14180.1. Different initiation.
AL450283, AL160270 Genomic DNA. Translation: CAI14181.1.
AL450283, AL160270 Genomic DNA. Translation: CAI14182.1.
CH471071 Genomic DNA. Translation: EAW58440.1.
CH471071 Genomic DNA. Translation: EAW58441.1.
BC000319 mRNA. Translation: AAH00319.1.
BC003004 mRNA. Translation: AAH03004.1.
BC107697 mRNA. Translation: AAI07698.1.
CCDSiCCDS65028.1. [O75935-3]
CCDS6560.1. [O75935-1]
CCDS6561.1. [O75935-2]
RefSeqiNP_001268354.1. NM_001281425.1. [O75935-3]
NP_001268355.1. NM_001281426.1.
NP_009165.1. NM_007234.4. [O75935-1]
NP_077324.1. NM_024348.3. [O75935-2]
UniGeneiHs.511768.

Genome annotation databases

EnsembliENST00000259632; ENSP00000259632; ENSG00000137100. [O75935-1]
ENST00000341694; ENSP00000343986; ENSG00000137100. [O75935-2]
ENST00000378916; ENSP00000368196; ENSG00000137100. [O75935-3]
GeneIDi11258.
KEGGihsa:11258.
UCSCiuc003zuw.3. human. [O75935-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF082513 mRNA. Translation: AAC61280.1.
CR541889 mRNA. Translation: CAG46687.1.
AK314730 mRNA. Translation: BAG37272.1.
AL160270, AL450283 Genomic DNA. Translation: CAI13143.1.
AL160270, AL450283 Genomic DNA. Translation: CAI13144.1. Different initiation.
AL160270, AL450283 Genomic DNA. Translation: CAI13145.1.
AL450283 Genomic DNA. Translation: CAI14178.1. Sequence problems.
AL450283, AL160270 Genomic DNA. Translation: CAI14180.1. Different initiation.
AL450283, AL160270 Genomic DNA. Translation: CAI14181.1.
AL450283, AL160270 Genomic DNA. Translation: CAI14182.1.
CH471071 Genomic DNA. Translation: EAW58440.1.
CH471071 Genomic DNA. Translation: EAW58441.1.
BC000319 mRNA. Translation: AAH00319.1.
BC003004 mRNA. Translation: AAH03004.1.
BC107697 mRNA. Translation: AAI07698.1.
CCDSiCCDS65028.1. [O75935-3]
CCDS6560.1. [O75935-1]
CCDS6561.1. [O75935-2]
RefSeqiNP_001268354.1. NM_001281425.1. [O75935-3]
NP_001268355.1. NM_001281426.1.
NP_009165.1. NM_007234.4. [O75935-1]
NP_077324.1. NM_024348.3. [O75935-2]
UniGeneiHs.511768.

3D structure databases

ProteinModelPortaliO75935.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116418. 47 interactors.
IntActiO75935. 21 interactors.
MINTiMINT-1033946.
STRINGi9606.ENSP00000259632.

PTM databases

iPTMnetiO75935.
PhosphoSitePlusiO75935.

Proteomic databases

EPDiO75935.
MaxQBiO75935.
PaxDbiO75935.
PeptideAtlasiO75935.
PRIDEiO75935.
TopDownProteomicsiO75935-1. [O75935-1]
O75935-3. [O75935-3]

Protocols and materials databases

DNASUi11258.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000259632; ENSP00000259632; ENSG00000137100. [O75935-1]
ENST00000341694; ENSP00000343986; ENSG00000137100. [O75935-2]
ENST00000378916; ENSP00000368196; ENSG00000137100. [O75935-3]
GeneIDi11258.
KEGGihsa:11258.
UCSCiuc003zuw.3. human. [O75935-1]

Organism-specific databases

CTDi11258.
DisGeNETi11258.
GeneCardsiDCTN3.
HGNCiHGNC:2713. DCTN3.
HPAiHPA044905.
MIMi607387. gene.
neXtProtiNX_O75935.
OpenTargetsiENSG00000137100.
PharmGKBiPA27182.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IXN7. Eukaryota.
ENOG410Y8FP. LUCA.
GeneTreeiENSGT00390000016210.
HOGENOMiHOG000231666.
HOVERGENiHBG053181.
InParanoidiO75935.
KOiK10425.
OMAiYNKMMFL.
OrthoDBiEOG091G0MGW.
PhylomeDBiO75935.
TreeFamiTF105248.

Enzyme and pathway databases

ReactomeiR-HSA-2132295. MHC class II antigen presentation.
R-HSA-2565942. Regulation of PLK1 Activity at G2/M Transition.
R-HSA-380259. Loss of Nlp from mitotic centrosomes.
R-HSA-380270. Recruitment of mitotic centrosome proteins and complexes.
R-HSA-5620912. Anchoring of the basal body to the plasma membrane.
R-HSA-6807878. COPI-mediated anterograde transport.
R-HSA-6811436. COPI-independent Golgi-to-ER retrograde traffic.
R-HSA-8854518. AURKA Activation by TPX2.

Miscellaneous databases

GeneWikiiDCTN3.
GenomeRNAii11258.
PROiO75935.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000137100.
CleanExiHS_DCTN3.
ExpressionAtlasiO75935. baseline and differential.
GenevisibleiO75935. HS.

Family and domain databases

InterProiIPR009991. Dynactin_p22.
[Graphical view]
PfamiPF07426. Dynactin_p22. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDCTN3_HUMAN
AccessioniPrimary (citable) accession number: O75935
Secondary accession number(s): A6NII7
, B2RBM5, Q5T1I5, Q5T1I7, Q5T8G3, Q9BPU8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: November 1, 1998
Last modified: November 30, 2016
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.