O75935 (DCTN3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dynactin subunit 3

Alternative name(s):
Dynactin complex subunit 22 kDa subunit
Short name=p22

Gene names

Name:	DCTN3
Synonyms:	DCTN22

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	186 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Together with dynein may be involved in spindle assembly and cytokinesis. Ref.1
Subunit structure	Subunit of dynactin, a multiprotein complex associated with dynein.
Subcellular location	Cytoplasm. Cytoplasm › cytoskeleton › centrosome. Chromosome › centromere › kinetochore. Cytoplasm › cytoskeleton › spindle. Cleavage furrow. Midbody. Note: Localizes to punctate cytoplasmic structures and to the centrosome during interphase, and to kinetochores and to spindle poles throughout mitosis. Colocalizes with dynein to the cleavage furrow and to midbody of dividing cells. Ref.1
Tissue specificity	Ubiquitously expressed. Highly expressed in muscle and pancreas and detected at lower levels in brain. Ref.1
Sequence similarities	Belongs to the dynactin subunit 3 family.
Sequence caution	The sequence CAI13144.1 differs from that shown. Reason: Erroneous initiation. The sequence CAI14178.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAI14180.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Cellular component	Centromere Chromosome Cytoplasm Cytoskeleton Kinetochore
Coding sequence diversity	Alternative splicing
Domain	Coiled coil
PTM	Acetylation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	G2/M transition of mitotic cell cycle Traceable author statement. Source: Reactome cytokinesis Inferred from direct assay Ref.1. Source: UniProtKB mitosis Traceable author statement. Source: ProtInc
Cellular component	centrosome Inferred from direct assay. Source: UniProtKB cleavage furrow Inferred from electronic annotation. Source: UniProtKB-SubCell condensed chromosome kinetochore Inferred from electronic annotation. Source: UniProtKB-SubCell cytosol Traceable author statement. Source: Reactome dynactin complex Inferred from physical interaction Ref.1. Source: UniProtKB midbody Inferred from electronic annotation. Source: UniProtKB-SubCell perinuclear region of cytoplasm Inferred from direct assay Ref.1. Source: UniProtKB spindle Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction Ref.1. Source: UniProtKB structural molecule activity Traceable author statement. Source: ProtInc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PBX2	P40425	1	EBI-347442,EBI-348489

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 Ref.1 (identifier: O75935-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 Ref.4 (identifier: O75935-2)

The sequence of this isoform differs from the canonical sequence as follows:
     138-186: DQCVEITEES...AATQVKPAEE → APWGVGVRDE...GPTGPVCGNH

Note: No experimental confirmation available.

Isoform 3 (identifier: O75935-3)

The sequence of this isoform differs from the canonical sequence as follows:
     90-117: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 186	185	Dynactin subunit 3		PRO_0000225603
Regions
	Coiled coil	135 – 157	23	Potential
Amino acid modifications
	Modified residue	2	1	N-acetylalanine Ref.7
Natural variations
	Alternative sequence	90 – 117	28	Missing in isoform 3.		VSP_051961
	Alternative sequence	138 – 186	49	DQCVE…KPAEE → APWGVGVRDEAGSLVEDVGF AQFLSVLHFGPTGPVCGNH in isoform 2. Ref.4		VSP_051964

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 70A0B26E24603A77	FASTA	186	21,119
	10 20 30 40 50 60 MAGLTDLQRL QARVEELERW VYGPGGARGS RKVADGLVKV QVALGNISSK RERVKILYKK 70 80 90 100 110 120 IEDLIKYLDP EYIDRIAIPD ASKLQFILAE EQFILSQVAL LEQVNALVPM LDSAHIKAVP 130 140 150 160 170 180 EHAARLQRLA QIHIQQQDQC VEITEESKAL LEEYNKTTML LSKQFVQWDE LLCQLEAATQ VKPAEE
	Isoform 2 [UniParc]. Checksum: FE50412C172E1922	FASTA	176	19,469
	10 20 30 40 50 60 MAGLTDLQRL QARVEELERW VYGPGGARGS RKVADGLVKV QVALGNISSK RERVKILYKK 70 80 90 100 110 120 IEDLIKYLDP EYIDRIAIPD ASKLQFILAE EQFILSQVAL LEQVNALVPM LDSAHIKAVP 130 140 150 160 170 EHAARLQRLA QIHIQQQAPW GVGVRDEAGS LVEDVGFAQF LSVLHFGPTG PVCGNH
	Isoform 3 [UniParc]. Checksum: 218ABF3564911AB1	FASTA	158	18,001
	10 20 30 40 50 60 MAGLTDLQRL QARVEELERW VYGPGGARGS RKVADGLVKV QVALGNISSK RERVKILYKK 70 80 90 100 110 120 IEDLIKYLDP EYIDRIAIPD ASKLQFILAA VPEHAARLQR LAQIHIQQQD QCVEITEESK 130 140 150 ALLEEYNKTT MLLSKQFVQW DELLCQLEAA TQVKPAEE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the p22 subunit of dynactin reveals the localization of cytoplasmic dynein and dynactin to the midbody of dividing cells." Karki S., LaMonte B., Holzbaur E.L.F. J. Cell Biol. 142:1023-1034(1998) [PubMed: 9722614] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PROTEIN SEQUENCE OF 20-28 AND 67-75, SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Tissue: Neuron.
[2]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[4]	"DNA sequence and analysis of human chromosome 9." Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I. Nature 429:369-374(2004) [PubMed: 15164053] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Lung.
[7]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[8]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF082513 mRNA. Translation: AAC61280.1. CR541889 mRNA. Translation: CAG46687.1. AK314730 mRNA. Translation: BAG37272.1. AL160270, AL450283 Genomic DNA. Translation: CAI13143.1. AL160270, AL450283 Genomic DNA. Translation: CAI13144.1. Different initiation. AL160270, AL450283 Genomic DNA. Translation: CAI13145.1. AL450283 Genomic DNA. Translation: CAI14178.1. Sequence problems. AL450283, AL160270 Genomic DNA. Translation: CAI14180.1. Different initiation. AL450283, AL160270 Genomic DNA. Translation: CAI14181.1. AL450283, AL160270 Genomic DNA. Translation: CAI14182.1. CH471071 Genomic DNA. Translation: EAW58440.1. CH471071 Genomic DNA. Translation: EAW58441.1. BC000319 mRNA. Translation: AAH00319.1. BC003004 mRNA. Translation: AAH03004.1. BC107697 mRNA. Translation: AAI07698.1.
IPI	IPI00013654. IPI00027014. IPI00514971.
RefSeq	NP_009165.1. NM_007234.3. NP_077324.1. NM_024348.2.
UniGene	Hs.511768.
3D structure databases
ProteinModelPortal	O75935.
ModBase	Search...
Protein-protein interaction databases
IntAct	O75935. 8 interactions.
MINT	MINT-1033946.
STRING	O75935.
PTM databases
PhosphoSite	O75935.
Proteomic databases
PRIDE	O75935.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000259632; ENSP00000259632; ENSG00000137100.
GeneID	11258.
KEGG	hsa:11258.
UCSC	uc003zuw.1. human. uc003zux.1. human.
Organism-specific databases
CTD	11258.
GeneCards	GC09M034569.
H-InvDB	HIX0007999.
HGNC	HGNC:2713. DCTN3.
HPA	HPA044905.
MIM	607387. gene.
neXtProt	NX_O75935.
PharmGKB	PA27182.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG18454.
GeneTree	ENSGT00390000016210.
HOGENOM	HBG716675.
HOVERGEN	HBG053181.
InParanoid	O75935.
OMA	QCMEITE.
OrthoDB	EOG4J9N11.
PhylomeDB	O75935.
Enzyme and pathway databases
Reactome	REACT_152. Cell Cycle, Mitotic.
Gene expression databases
ArrayExpress	O75935.
Bgee	O75935.
CleanEx	HS_DCTN3.
Genevestigator	O75935.
GermOnline	ENSG00000137100. Homo sapiens.
Family and domain databases
InterPro	IPR009991. Dynactin_p22. [Graphical view]
KO	K10425.
Pfam	PF07426. Dynactin_p22. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	42838.
SOURCE	Search...

Entry information

Entry name

DCTN3_HUMAN

Accession

Primary (citable) accession number: O75935
Secondary accession number(s): A6NII7 , B2RBM5, Q5T1I5, Q5T1I7, Q5T8G3, Q9BPU8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	November 1, 1998
Last modified:	January 25, 2012
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.