BCAS2

Functionⁱ

Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. May have a scaffolding role in the spliceosome assembly as it contacts all other components of the core complex. The PRP19-CDC5L complex may also play a role in the response to DNA damage (DDR).1 Publication

GO - Biological processⁱ

mRNA splicing, via spliceosome Source: Reactome
RNA splicing
Source: UniProtKB
Traceable author statementⁱ
- 9731529
RNA splicing, via transesterification reactions
Source: UniProtKB
Traceable author statementⁱ
- 9731529

Complete GO annotation...

Keywordsⁱ

Biological process	mRNA processing, mRNA splicing

Biological process

mRNA processing, mRNA splicing

Enzyme and pathway databases

Reactomeⁱ	R-HSA-72163. mRNA Splicing - Major Pathway.

Reactomeⁱ

R-HSA-72163. mRNA Splicing - Major Pathway.

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Pre-mRNA-splicing factor SPF27 Alternative name(s): Breast carcinoma-amplified sequence 2 DNA amplified in mammary carcinoma 1 protein Spliceosome-associated protein SPF 27
Gene namesⁱ	Name:BCAS2 Synonyms:DAM1
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 1

Organism-specific databases

Human Gene Nomenclature Database More...HGNCⁱ	HGNC:975. BCAS2.

HGNCⁱ

HGNC:975. BCAS2.

Subcellular locationⁱ

Nucleus › nucleolus
3 Publications
Manual assertion based on experiment inⁱ
- Ref.8
  "Amplification of the BCAS2 gene at chromosome 1p13.3-21 in human primary breast cancer."
  Maass N., Rosel F., Schem C., Hitomi J., Jonat W., Nagasaki K.
  Cancer Lett. 185:219-223(2002) [PubMed] [Europe PMC] [Abstract]
  Cited for: SUBCELLULAR LOCATION.
- Ref.9
  "Functional proteomic analysis of human nucleolus."
  Scherl A., Coute Y., Deon C., Calle A., Kindbeiter K., Sanchez J.-C., Greco A., Hochstrasser D.F., Diaz J.-J.
  Mol. Biol. Cell 13:4100-4109(2002) [PubMed] [Europe PMC] [Abstract]
  Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
- Ref.11
  "Molecular architecture of the human Prp19/CDC5L complex."
  Grote M., Wolf E., Will C.L., Lemm I., Agafonov D.E., Schomburg A., Fischle W., Urlaub H., Luhrmann R.
  Mol. Cell. Biol. 30:2105-2119(2010) [PubMed] [Europe PMC] [Abstract]
  Cited for: IDENTIFICATION AS A COMPONENT OF THE PRP19-CDC5L SPLICING COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION, INTERACTION WITH CDC5L; PLRG1 AND PRPF19.

GO - Cellular componentⁱ

catalytic step 2 spliceosome Source: GO_Central
centrosome Source: HPA
nuclear speck Source: HPA
nucleolus Source: UniProtKB-SubCell
nucleoplasm Source: Reactome
precatalytic spliceosome Source: GO_Central
Prp19 complex Source: GO_Central
spliceosomal complex
Source: MGI
Inferred from direct assayⁱ
- 12169396

Complete GO annotation...

Keywords - Cellular componentⁱ

Nucleus, Spliceosome

Pathology & Biotechⁱ

Organism-specific databases

DisGeNET More...DisGeNETⁱ	10286.
Open Targets More...OpenTargetsⁱ	ENSG00000116752.
PharmGKBⁱ	PA25285.

Polymorphism and mutation databases

BioMutaⁱ	BCAS2.

BioMutaⁱ

BCAS2.

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Initiator methionineⁱ		RemovedCombined sources1 Publication
ChainⁱPRO_0000064861	2 – 225	Pre-mRNA-splicing factor SPF27Add BLAST		224

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Modified residueⁱ	2	N-acetylalanineCombined sources1 Publication		1
Modified residueⁱ	94	PhosphoserineCombined sources		1

Keywords - PTMⁱ

Acetylation, Phosphoprotein

Proteomic databases

EPDⁱ	O75934.
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	O75934.
PaxDbⁱ	O75934.
PeptideAtlas More...PeptideAtlasⁱ	O75934.
PRIDEⁱ	O75934.
TopDownProteomicsⁱ	O75934.

2D gel databases

SWISS-2DPAGEⁱ	O75934.

SWISS-2DPAGEⁱ

O75934.

PTM databases

iPTMnetⁱ	O75934.
PhosphoSitePlusⁱ	O75934.

Miscellaneous databases

PMAP-CutDBⁱ	O75934.

PMAP-CutDBⁱ

O75934.

Expressionⁱ

Tissue specificityⁱ

Ubiquitously expressed.1 Publication

Gene expression databases

Bgeeⁱ	ENSG00000116752.
CleanExⁱ	HS_BCAS2.
ExpressionAtlasⁱ	O75934. baseline and differential.
Genevisibleⁱ	O75934. HS.

Organism-specific databases

Human Protein Atlas More...HPAⁱ	HPA067881.

HPAⁱ

HPA067881.

Interactionⁱ

Subunit structureⁱ

Component of the PRP19-CDC5L splicing complex composed of a core complex comprising a homotetramer of PRPF19, CDC5L, PLRG1 and BCAS2, and at least three less stably associated proteins CTNNBL1, CWC15 and HSPA8. Interacts directly in the complex with PRPF19, CDC5L and PLRG1.2 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct
AIMP2	Q13155	7	EBI-1050106,EBI-745226
GOLGA2	A0A0C4DGS5	4	EBI-1050106,EBI-11522202
GOLGA2	Q08379	3	EBI-1050106,EBI-618309
HGS	O14964	3	EBI-1050106,EBI-740220
IKZF3	Q9UKT9	3	EBI-1050106,EBI-747204
KRT34	O76011	4	EBI-1050106,EBI-1047093
KRT40	Q6A162	3	EBI-1050106,EBI-10171697
LMO2	P25791-3	4	EBI-1050106,EBI-11959475
LMO4	P61968	4	EBI-1050106,EBI-2798728
PRPF19	Q9UMS4	2	EBI-1050106,EBI-395746
PRPF8	Q6P2Q9	2	EBI-1050106,EBI-538479
SF3B4	Q15427	2	EBI-1050106,EBI-348469
TCF4	P15884	3	EBI-1050106,EBI-533224

Show more details

Protein-protein interaction databases

BioGridⁱ	115575. 82 interactors.
IntActⁱ	O75934. 70 interactors.
Molecular INTeraction database More...MINTⁱ	MINT-3002049.
STRINGⁱ	9606.ENSP00000358554.

Structureⁱ

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	5MQF	electron microscopy	5.90	K	1-225	[»]
ProteinModelPortalⁱ	O75934.
SMRⁱ	O75934.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Coiled coil

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Coiled coilⁱ	138 – 222	Sequence analysisAdd BLAST		85

Sequence similaritiesⁱ

Belongs to the SPF27 family.Curated

Keywords - Domainⁱ

Coiled coil

Phylogenomic databases

eggNOGⁱ	KOG3096. Eukaryota. ENOG41101IB. LUCA.
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000014494.
HOGENOMⁱ	HOG000007540.
HOVERGENⁱ	HBG050675.
InParanoidⁱ	O75934.
KEGG Orthology (KO) More...KOⁱ	K12861.
OMAⁱ	LPYIDHG.
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0NMJ.
PhylomeDBⁱ	O75934.
TreeFamⁱ	TF105818.

Family and domain databases

InterProⁱ	View protein in InterPro IPR008409. SPF27.
PANTHERⁱ	PTHR13296:SF1. PTHR13296:SF1. 1 hit.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF05700. BCAS2. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

O75934-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MAGTGLVAGE VVVDALPYFD QGYEAPGVRE AAAALVEEET RRYRPTKNYL 
        60         70         80         90        100
SYLTAPDYSA FETDIMRNEF ERLAARQPIE LLSMKRYELP APSSGQKNDI 
       110        120        130        140        150
TAWQECVNNS MAQLEHQAVR IENLELMSQH GCNAWKVYNE NLVHMIEHAQ 
       160        170        180        190        200
KELQKLRKHI QDLNWQRKNM QLTAGSKLRE MESNWVSLVS KNYEIERTIV 
       210        220 
QLENEIYQIK QQHGEANKEN IRQDF

Length:225

Mass (Da):26,131

Last modified:November 1, 1998 - v1

Checksum:ⁱ9112718EEFD96890

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	24	E → D in CAG46834 (Ref. 3) Curated		1
Sequence conflictⁱ	89	L → V in CAG46834 (Ref. 3) Curated		1

Natural variant

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Natural variantⁱVAR_035799	139	N → S in a colorectal cancer sample; somatic mutation. 1 Publication		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF081788 mRNA. Translation: AAC64059.1. AB020623 mRNA. Translation: BAA34863.1. CR542037 mRNA. Translation: CAG46834.1. BT019390 mRNA. Translation: AAV38197.1. AL390241 Genomic DNA. No translation available. BC005285 mRNA. Translation: AAH05285.1. BC012623 mRNA. Translation: AAH12623.1. BC022880 mRNA. Translation: AAH22880.1.
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS874.1.
NCBI Reference Sequences More...RefSeqⁱ	NP_005863.1. NM_005872.2.
UniGeneⁱ	Hs.22960.

Genome annotation databases

Ensemblⁱ	ENST00000369541; ENSP00000358554; ENSG00000116752.
GeneIDⁱ	10286.
KEGGⁱ	hsa:10286.
UCSC genome browser More...UCSCⁱ	uc001efa.4. human.

Keywords - Coding sequence diversityⁱ

Polymorphism

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF081788 mRNA. Translation: AAC64059.1. AB020623 mRNA. Translation: BAA34863.1. CR542037 mRNA. Translation: CAG46834.1. BT019390 mRNA. Translation: AAV38197.1. AL390241 Genomic DNA. No translation available. BC005285 mRNA. Translation: AAH05285.1. BC012623 mRNA. Translation: AAH12623.1. BC022880 mRNA. Translation: AAH22880.1.
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS874.1.
NCBI Reference Sequences More...RefSeqⁱ	NP_005863.1. NM_005872.2.
UniGeneⁱ	Hs.22960.

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	5MQF	electron microscopy	5.90	K	1-225	[»]
ProteinModelPortalⁱ	O75934.
SMRⁱ	O75934.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	115575. 82 interactors.
IntActⁱ	O75934. 70 interactors.
Molecular INTeraction database More...MINTⁱ	MINT-3002049.
STRINGⁱ	9606.ENSP00000358554.

PTM databases

iPTMnetⁱ	O75934.
PhosphoSitePlusⁱ	O75934.

Polymorphism and mutation databases

BioMutaⁱ	BCAS2.

BioMutaⁱ

BCAS2.

2D gel databases

SWISS-2DPAGEⁱ	O75934.

SWISS-2DPAGEⁱ

O75934.

Proteomic databases

EPDⁱ	O75934.
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	O75934.
PaxDbⁱ	O75934.
PeptideAtlas More...PeptideAtlasⁱ	O75934.
PRIDEⁱ	O75934.
TopDownProteomicsⁱ	O75934.

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	10286.
Structural Biology Knowledgebase	Search...

Genome annotation databases

Ensemblⁱ	ENST00000369541; ENSP00000358554; ENSG00000116752.
GeneIDⁱ	10286.
KEGGⁱ	hsa:10286.
UCSC genome browser More...UCSCⁱ	uc001efa.4. human.

Organism-specific databases

CTDⁱ	10286.
DisGeNET More...DisGeNETⁱ	10286.
GeneCardsⁱ	BCAS2.
H-InvDBⁱ	HIX0077639.
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:975. BCAS2.
Human Protein Atlas More...HPAⁱ	HPA067881.
MIMⁱ	605783. gene.
neXtProtⁱ	NX_O75934.
Open Targets More...OpenTargetsⁱ	ENSG00000116752.
PharmGKBⁱ	PA25285.
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	KOG3096. Eukaryota. ENOG41101IB. LUCA.
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000014494.
HOGENOMⁱ	HOG000007540.
HOVERGENⁱ	HBG050675.
InParanoidⁱ	O75934.
KEGG Orthology (KO) More...KOⁱ	K12861.
OMAⁱ	LPYIDHG.
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0NMJ.
PhylomeDBⁱ	O75934.
TreeFamⁱ	TF105818.

Enzyme and pathway databases

Reactomeⁱ	R-HSA-72163. mRNA Splicing - Major Pathway.

Reactomeⁱ

R-HSA-72163. mRNA Splicing - Major Pathway.

Miscellaneous databases

GeneWikiⁱ	BCAS2.
GenomeRNAiⁱ	10286.
PMAP-CutDBⁱ	O75934.
Protein Ontology More...PROⁱ	PR:O75934.
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000116752.
CleanExⁱ	HS_BCAS2.
ExpressionAtlasⁱ	O75934. baseline and differential.
Genevisibleⁱ	O75934. HS.

Family and domain databases

InterProⁱ	View protein in InterPro IPR008409. SPF27.
PANTHERⁱ	PTHR13296:SF1. PTHR13296:SF1. 1 hit.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF05700. BCAS2. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	SPF27_HUMAN
Accessionⁱ	O75934Primary (citable) accession number: O75934 Secondary accession number(s): Q6FGS0
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
	Last sequence update:	November 1, 1998
	Last modified:	June 7, 2017
	This is version 151 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM
Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations
Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
PDB cross-references
Index of Protein Data Bank (PDB) cross-references
SIMILARITY comments
Index of protein domains and families

Similar proteinsⁱ

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:

100%	UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%	UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%	UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - O75934 (SPF27_HUMAN)

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Pre-mRNA-splicing factor SPF27

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Organism-specific databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Proteomic databases

2D gel databases

PTM databases

Miscellaneous databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Organism-specific databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Coiled coil

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including length and molecular weight.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

Experimental Info

Natural variant

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Polymorphism and mutation databases

2D gel databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

Functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequenceⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ