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O75915

- PRAF3_HUMAN

UniProt

O75915 - PRAF3_HUMAN

Protein

PRA1 family protein 3

Gene

ARL6IP5

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 1 (01 Nov 1998)
      Previous versions | rss
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    Functioni

    Regulates intracellular concentrations of taurine and glutamate. Negatively modulates SLC1A1/EAAC1 glutamate transport activity by decreasing its affinity for glutamate. May be involved in membrane traffic By similarity.By similarity

    GO - Biological processi

    1. intrinsic apoptotic signaling pathway in response to oxidative stress Source: UniProt
    2. L-glutamate transport Source: UniProtKB
    3. negative regulation of mitochondrial membrane potential Source: UniProt
    4. negative regulation of transport Source: Ensembl
    5. positive regulation of apoptotic process Source: UniProt
    6. positive regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProt
    7. positive regulation of stress-activated MAPK cascade Source: UniProt

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    PRA1 family protein 3
    Alternative name(s):
    ADP-ribosylation factor-like protein 6-interacting protein 5
    Short name:
    ARL-6-interacting protein 5
    Short name:
    Aip-5
    Cytoskeleton-related vitamin A-responsive protein
    Dermal papilla-derived protein 11
    GTRAP3-18
    Glutamate transporter EAAC1-interacting protein
    JM5
    Prenylated Rab acceptor protein 2
    Protein JWa
    Putative MAPK-activating protein PM27
    Gene namesi
    Name:ARL6IP5
    Synonyms:DERP11, JWA, PRA2, PRAF3
    ORF Names:HSPC127
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 3

    Organism-specific databases

    HGNCiHGNC:16937. ARL6IP5.

    Subcellular locationi

    Endoplasmic reticulum membrane By similarity; Multi-pass membrane protein By similarity. Cytoplasm By similarity
    Note: Also exists as a soluble form in the cytoplasm. Associated with microtubules By similarity.By similarity

    GO - Cellular componenti

    1. endoplasmic reticulum Source: HPA
    2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    3. extracellular vesicular exosome Source: UniProt
    4. integral component of membrane Source: UniProtKB-KW
    5. membrane Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Endoplasmic reticulum, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA134898937.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 188188PRA1 family protein 3PRO_0000220883Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionine3 Publications

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiO75915.
    PaxDbiO75915.
    PeptideAtlasiO75915.
    PRIDEiO75915.

    PTM databases

    PhosphoSiteiO75915.

    Expressioni

    Inductioni

    By methyl-beta-cyclodextrin By similarity. Up-regulated upon induced differentiation and in heat stress.By similarity1 Publication

    Gene expression databases

    ArrayExpressiO75915.
    BgeeiO75915.
    CleanExiHS_ARL6IP5.
    GenevestigatoriO75915.

    Organism-specific databases

    HPAiCAB019321.
    HPA015540.

    Interactioni

    Subunit structurei

    Forms multimers. Binds to prenylated RAB1A and RAB3A, to the neuronal glutamate transporter SLC1A1/EAAC1, and to the ADP-ribosylation like factor ARL6, a member of the Ras superfamily. Does not bind to VAMP2 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi115801. 13 interactions.
    IntActiO75915. 1 interaction.
    MINTiMINT-1339938.
    STRINGi9606.ENSP00000273258.

    Structurei

    3D structure databases

    ProteinModelPortaliO75915.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 3535CytoplasmicBy similarityAdd
    BLAST
    Topological domaini78 – 9316CytoplasmicBy similarityAdd
    BLAST
    Topological domaini136 – 18853CytoplasmicBy similarityAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei36 – 5621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei57 – 7721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei94 – 11421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei115 – 13521HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni136 – 18853Targeting to endoplasmic reticulum membraneBy similarityAdd
    BLAST

    Sequence similaritiesi

    Belongs to the PRA1 family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG272388.
    HOGENOMiHOG000231363.
    HOVERGENiHBG053661.
    InParanoidiO75915.
    OMAiKMNKIQD.
    PhylomeDBiO75915.
    TreeFamiTF105479.

    Family and domain databases

    InterProiIPR004895. Prenylated_rab_accept_PRA1.
    [Graphical view]
    PfamiPF03208. PRA1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O75915-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDVNIAPLRA WDDFFPGSDR FARPDFRDIS KWNNRVVSNL LYYQTNYLVV    50
    AAMMISIVGF LSPFNMILGG IVVVLVFTGF VWAAHNKDVL RRMKKRYPTT 100
    FVMVVMLASY FLISMFGGVM VFVFGITFPL LLMFIHASLR LRNLKNKLEN 150
    KMEGIGLKRT PMGIVLDALE QQEEGINRLT DYISKVKE 188
    Length:188
    Mass (Da):21,615
    Last modified:November 1, 1998 - v1
    Checksum:i3AA708C6D0901B44
    GO

    Sequence cautioni

    The sequence BAD97286.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti28 – 281D → G in BAD97286. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB052638 mRNA. Translation: BAD83603.1.
    AY102608 mRNA. Translation: AAM28950.1.
    AB014765 mRNA. Translation: BAC66462.1.
    AF070523 mRNA. Translation: AAC64360.1.
    AF115965 mRNA. Translation: AAP97237.1.
    AF125530 mRNA. Translation: AAF17224.1.
    AF161476 mRNA. Translation: AAF29091.1.
    AB097051 mRNA. Translation: BAC77404.1.
    AK223566 mRNA. Translation: BAD97286.1. Different initiation.
    AK312731 mRNA. Translation: BAG35602.1.
    CH471055 Genomic DNA. Translation: EAW65473.1.
    BC005143 mRNA. Translation: AAH05143.1.
    BC020797 mRNA. Translation: AAH20797.1.
    CCDSiCCDS2912.1.
    RefSeqiNP_006398.1. NM_006407.3.
    UniGeneiHs.518060.
    Hs.745255.

    Genome annotation databases

    EnsembliENST00000273258; ENSP00000273258; ENSG00000144746.
    GeneIDi10550.
    KEGGihsa:10550.
    UCSCiuc003dnr.3. human.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB052638 mRNA. Translation: BAD83603.1 .
    AY102608 mRNA. Translation: AAM28950.1 .
    AB014765 mRNA. Translation: BAC66462.1 .
    AF070523 mRNA. Translation: AAC64360.1 .
    AF115965 mRNA. Translation: AAP97237.1 .
    AF125530 mRNA. Translation: AAF17224.1 .
    AF161476 mRNA. Translation: AAF29091.1 .
    AB097051 mRNA. Translation: BAC77404.1 .
    AK223566 mRNA. Translation: BAD97286.1 . Different initiation.
    AK312731 mRNA. Translation: BAG35602.1 .
    CH471055 Genomic DNA. Translation: EAW65473.1 .
    BC005143 mRNA. Translation: AAH05143.1 .
    BC020797 mRNA. Translation: AAH20797.1 .
    CCDSi CCDS2912.1.
    RefSeqi NP_006398.1. NM_006407.3.
    UniGenei Hs.518060.
    Hs.745255.

    3D structure databases

    ProteinModelPortali O75915.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 115801. 13 interactions.
    IntActi O75915. 1 interaction.
    MINTi MINT-1339938.
    STRINGi 9606.ENSP00000273258.

    PTM databases

    PhosphoSitei O75915.

    Proteomic databases

    MaxQBi O75915.
    PaxDbi O75915.
    PeptideAtlasi O75915.
    PRIDEi O75915.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000273258 ; ENSP00000273258 ; ENSG00000144746 .
    GeneIDi 10550.
    KEGGi hsa:10550.
    UCSCi uc003dnr.3. human.

    Organism-specific databases

    CTDi 10550.
    GeneCardsi GC03P069216.
    HGNCi HGNC:16937. ARL6IP5.
    HPAi CAB019321.
    HPA015540.
    MIMi 605709. gene.
    neXtProti NX_O75915.
    PharmGKBi PA134898937.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG272388.
    HOGENOMi HOG000231363.
    HOVERGENi HBG053661.
    InParanoidi O75915.
    OMAi KMNKIQD.
    PhylomeDBi O75915.
    TreeFami TF105479.

    Miscellaneous databases

    ChiTaRSi ARL6IP5. human.
    GeneWikii ARL6IP5.
    GenomeRNAii 10550.
    NextBioi 40017.
    PROi O75915.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O75915.
    Bgeei O75915.
    CleanExi HS_ARL6IP5.
    Genevestigatori O75915.

    Family and domain databases

    InterProi IPR004895. Prenylated_rab_accept_PRA1.
    [Graphical view ]
    Pfami PF03208. PRA1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of addicsin/GTRAP3-18 as a chronic morphine-augumented gene in amygdala."
      Ikemoto M.J., Inoue K., Akiduki S., Osugi T., Imamura T., Ishida N., Ohtomi M.
      NeuroReport 13:2079-2084(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "JM4 is a four-transmembrane protein binding to the CCR5 receptor."
      Schweneker M., Bachmann A.S., Moelling K.
      FEBS Lett. 579:1751-1758(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Neuroblastoma.
    3. "Molecular cloning of a dermal papilla derived gene."
      Ikeda A., Yoshimoto M.
      Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Hair follicle dermal papilla.
    4. "A novel differentially displayed vitamin A responsive gene that is cytoskeleton related."
      Zhou J.W., Di Y.P., Wu R.
      Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    5. "Cloning of a novel human cDNA homologous to human mRNA for JM4 protein."
      Zhang X.N., Yu L., Jin L., Hu P.R., Chen S.Y., Zhao S.Y.
      Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    6. "A novel gene expressed in the human adrenal gland."
      Jiang C., Huang C., Wu T., Peng Y., Gu Y., Zhang L., Zhang C., Li Y., Han Z., Wang Y., Chen Z., Fu G.
      Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Adrenal gland.
    7. "Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells."
      Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G., Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W., Tao J., Huang Q.-H., Zhou J., Hu G.-X.
      , Gu J., Chen S.-J., Chen Z.
      Genome Res. 10:1546-1560(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Umbilical cord blood.
    8. "Large-scale identification and characterization of human genes that activate NF-kappaB and MAPK signaling pathways."
      Matsuda A., Suzuki Y., Honda G., Muramatsu S., Matsuzaki O., Nagano Y., Doi T., Shimotohno K., Harada T., Nishida E., Hayashi H., Sugano S.
      Oncogene 22:3307-3318(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Lung fibroblast.
    9. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    10. Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
      Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Spleen.
    11. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    12. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Bone marrow and Brain.
    13. "Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides."
      Gevaert K., Goethals M., Martens L., Van Damme J., Staes A., Thomas G.R., Vandekerckhove J.
      Nat. Biotechnol. 21:566-569(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-9, ACETYLATION AT MET-1.
      Tissue: Platelet.
    14. Bienvenut W.V., Claeys D.
      Submitted (NOV-2005) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 1-27; 152-158 AND 160-185, ACETYLATION AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY.
      Tissue: Platelet.
    15. "Effect of differentiation inducer and heat stress on the expression of JWA protein and Hsp70 of K562 cells."
      Mao W.G., Li A.P., Ye J., Huang S., Li A.Q., Zhou J.W.
      Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi 21:253-256(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    16. "Genomic organization, expression profile, and characterization of the new protein PRA1 domain family, member 2 (PRAF2)."
      Fo C.S., Coleman C.S., Wallick C.J., Vine A.L., Bachmann A.S.
      Gene 371:154-165(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NOMENCLATURE.
    17. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    18. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
      Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
      Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    19. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiPRAF3_HUMAN
    AccessioniPrimary (citable) accession number: O75915
    Secondary accession number(s): B2R6V5, Q53ES3, Q5KU08
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 4, 2005
    Last sequence update: November 1, 1998
    Last modified: October 1, 2014
    This is version 111 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 3
      Human chromosome 3: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3