O75915 (PRAF3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
PRA1 family protein 3

Alternative name(s):
ADP-ribosylation factor-like protein 6-interacting protein 5
Short name=ARL-6-interacting protein 5
Short name=Aip-5
Cytoskeleton-related vitamin A-responsive protein
Dermal papilla-derived protein 11
GTRAP3-18
Glutamate transporter EAAC1-interacting protein
JM5
Prenylated Rab acceptor protein 2
Protein JWa
Putative MAPK-activating protein PM27

Gene names

Name:	ARL6IP5
Synonyms:	DERP11, JWA, PRA2, PRAF3
ORF Names:	HSPC127

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	188 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Regulates intracellular concentrations of taurine and glutamate. Negatively modulates SLC1A1/EAAC1 glutamate transport activity by decreasing its affinity for glutamate. May be involved in membrane traffic By similarity.
Subunit structure	Forms multimers. Binds to prenylated RAB1A and RAB3A, to the neuronal glutamate transporter SLC1A1/EAAC1, and to the ADP-ribosylation like factor ARL6, a member of the Ras superfamily. Does not bind to VAMP2 By similarity.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity. Cytoplasm By similarity. Note: Also exists as a soluble form in the cytoplasm. Associated with microtubules By similarity.
Induction	By methyl-beta-cyclodextrin By similarity. Up-regulated upon induced differentiation and in heat stress. Ref.15
Sequence similarities	Belongs to the PRA1 family.
Sequence caution	The sequence BAD97286.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasm Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
PTM	Acetylation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	L-glutamate transport Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of transport Inferred from electronic annotation. Source: Compara
Cellular_component	endoplasmic reticulum Inferred from direct assay. Source: HPA endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 188	188	PRA1 family protein 3		PRO_0000220883
Regions
	Topological domain	1 – 35	35	Cytoplasmic By similarity
	Transmembrane	36 – 56	21	Helical; Potential
	Transmembrane	57 – 77	21	Helical; Potential
	Topological domain	78 – 93	16	Cytoplasmic By similarity
	Transmembrane	94 – 114	21	Helical; Potential
	Transmembrane	115 – 135	21	Helical; Potential
	Topological domain	136 – 188	53	Cytoplasmic By similarity
	Region	136 – 188	53	Targeting to endoplasmic reticulum membrane By similarity
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine Ref.13 Ref.14
Experimental info
	Sequence conflict	28	1	D → G in BAD97286. Ref.10

Sequences

	Sequence		Length	Mass (Da)
	O75915 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 3AA708C6D0901B44	FASTA	188	21,615
	10 20 30 40 50 60 MDVNIAPLRA WDDFFPGSDR FARPDFRDIS KWNNRVVSNL LYYQTNYLVV AAMMISIVGF 70 80 90 100 110 120 LSPFNMILGG IVVVLVFTGF VWAAHNKDVL RRMKKRYPTT FVMVVMLASY FLISMFGGVM 130 140 150 160 170 180 VFVFGITFPL LLMFIHASLR LRNLKNKLEN KMEGIGLKRT PMGIVLDALE QQEEGINRLT DYISKVKE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of addicsin/GTRAP3-18 as a chronic morphine-augumented gene in amygdala." Ikemoto M.J., Inoue K., Akiduki S., Osugi T., Imamura T., Ishida N., Ohtomi M. NeuroReport 13:2079-2084(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"JM4 is a four-transmembrane protein binding to the CCR5 receptor." Schweneker M., Bachmann A.S., Moelling K. FEBS Lett. 579:1751-1758(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Neuroblastoma.
[3]	"Molecular cloning of a dermal papilla derived gene." Ikeda A., Yoshimoto M. Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Hair follicle dermal papilla.
[4]	"A novel differentially displayed vitamin A responsive gene that is cytoskeleton related." Zhou J.W., Di Y.P., Wu R. Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[5]	"Cloning of a novel human cDNA homologous to human mRNA for JM4 protein." Zhang X.N., Yu L., Jin L., Hu P.R., Chen S.Y., Zhao S.Y. Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[6]	"A novel gene expressed in the human adrenal gland." Jiang C., Huang C., Wu T., Peng Y., Gu Y., Zhang L., Zhang C., Li Y., Han Z., Wang Y., Chen Z., Fu G. Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Adrenal gland.
[7]	"Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells." Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G., Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W., Tao J., Huang Q.-H., Zhou J., Hu G.-X. , Gu J., Chen S.-J., Chen Z. Genome Res. 10:1546-1560(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Umbilical cord blood.
[8]	"Large-scale identification and characterization of human genes that activate NF-kappaB and MAPK signaling pathways." Matsuda A., Suzuki Y., Honda G., Muramatsu S., Matsuzaki O., Nagano Y., Doi T., Shimotohno K., Harada T., Nishida E., Hayashi H., Sugano S. Oncogene 22:3307-3318(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung fibroblast.
[9]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[10]	Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Spleen.
[11]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[12]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Bone marrow and Brain.
[13]	"Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides." Gevaert K., Goethals M., Martens L., Van Damme J., Staes A., Thomas G.R., Vandekerckhove J. Nat. Biotechnol. 21:566-569(2003) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-9, ACETYLATION AT MET-1. Tissue: Platelet.
[14]	Bienvenut W.V., Claeys D. Submitted (NOV-2005) to UniProtKB Cited for: PROTEIN SEQUENCE OF 1-27; 152-158 AND 160-185, ACETYLATION AT MET-1, MASS SPECTROMETRY. Tissue: Platelet.
[15]	"Effect of differentiation inducer and heat stress on the expression of JWA protein and Hsp70 of K562 cells." Mao W.G., Li A.P., Ye J., Huang S., Li A.Q., Zhou J.W. Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi 21:253-256(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
[16]	"Genomic organization, expression profile, and characterization of the new protein PRA1 domain family, member 2 (PRAF2)." Fo C.S., Coleman C.S., Wallick C.J., Vine A.L., Bachmann A.S. Gene 371:154-165(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NOMENCLATURE.
[17]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB052638 mRNA. Translation: BAD83603.1. AY102608 mRNA. Translation: AAM28950.1. AB014765 mRNA. Translation: BAC66462.1. AF070523 mRNA. Translation: AAC64360.1. AF115965 mRNA. Translation: AAP97237.1. AF125530 mRNA. Translation: AAF17224.1. AF161476 mRNA. Translation: AAF29091.1. AB097051 mRNA. Translation: BAC77404.1. AK223566 mRNA. Translation: BAD97286.1. Different initiation. AK312731 mRNA. Translation: BAG35602.1. CH471055 Genomic DNA. Translation: EAW65473.1. BC005143 mRNA. Translation: AAH05143.1. BC020797 mRNA. Translation: AAH20797.1.
IPI	IPI00007426.
RefSeq	NP_006398.1. NM_006407.3.
UniGene	Hs.518060. Hs.741289.
3D structure databases
ProteinModelPortal	O75915.
ModBase	Search...
Protein-protein interaction databases
IntAct	O75915. 1 interaction.
MINT	MINT-1339938.
STRING	9606.ENSP00000273258.
PTM databases
PhosphoSite	O75915.
Proteomic databases
PaxDb	O75915.
PeptideAtlas	O75915.
PRIDE	O75915.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000273258; ENSP00000273258; ENSG00000144746.
GeneID	10550.
KEGG	hsa:10550.
UCSC	uc003dnr.3. human.
Organism-specific databases
CTD	10550.
GeneCards	GC03P069216.
HGNC	HGNC:16937. ARL6IP5.
HPA	CAB019321. HPA015540.
MIM	605709. gene.
neXtProt	NX_O75915.
PharmGKB	PA134898937.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG272388.
HOGENOM	HOG000231363.
HOVERGEN	HBG053661.
InParanoid	O75915.
OMA	HNKDILR.
OrthoDB	EOG4H72CR.
PhylomeDB	O75915.
Gene expression databases
ArrayExpress	O75915.
Bgee	O75915.
CleanEx	HS_ARL6IP5.
Genevestigator	O75915.
GermOnline	ENSG00000144746. Homo sapiens.
Family and domain databases
InterPro	IPR004895. Prenylated_rab_accept_PRA1. [Graphical view]
Pfam	PF03208. PRA1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	ARL6IP5. human.
GenomeRNAi	10550.
NextBio	40017.
SOURCE	Search...

Entry information

Entry name

PRAF3_HUMAN

Accession

Primary (citable) accession number: O75915
Secondary accession number(s): B2R6V5, Q53ES3, Q5KU08

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 4, 2005
Last sequence update:	November 1, 1998
Last modified:	April 3, 2013
This is version 99 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.