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O75915 (PRAF3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
PRA1 family protein 3
Alternative name(s):
ADP-ribosylation factor-like protein 6-interacting protein 5
Short name=ARL-6-interacting protein 5
Short name=Aip-5
Cytoskeleton-related vitamin A-responsive protein
Dermal papilla-derived protein 11
GTRAP3-18
Glutamate transporter EAAC1-interacting protein
JM5
Prenylated Rab acceptor protein 2
Protein JWa
Putative MAPK-activating protein PM27
Gene names
Name:ARL6IP5
Synonyms:DERP11, JWA, PRA2, PRAF3
ORF Names:HSPC127
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length188 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Regulates intracellular concentrations of taurine and glutamate. Negatively modulates SLC1A1/EAAC1 glutamate transport activity by decreasing its affinity for glutamate. May be involved in membrane traffic By similarity.

Subunit structure

Forms multimers. Binds to prenylated RAB1A and RAB3A, to the neuronal glutamate transporter SLC1A1/EAAC1, and to the ADP-ribosylation like factor ARL6, a member of the Ras superfamily. Does not bind to VAMP2 By similarity.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity. Cytoplasm By similarity. Note: Also exists as a soluble form in the cytoplasm. Associated with microtubules By similarity.

Induction

By methyl-beta-cyclodextrin By similarity. Up-regulated upon induced differentiation and in heat stress. Ref.15

Sequence similarities

Belongs to the PRA1 family.

Sequence caution

The sequence BAD97286.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 188188PRA1 family protein 3
PRO_0000220883

Regions

Topological domain1 – 3535Cytoplasmic By similarity
Transmembrane36 – 5621Helical; Potential
Transmembrane57 – 7721Helical; Potential
Topological domain78 – 9316Cytoplasmic By similarity
Transmembrane94 – 11421Helical; Potential
Transmembrane115 – 13521Helical; Potential
Topological domain136 – 18853Cytoplasmic By similarity
Region136 – 18853Targeting to endoplasmic reticulum membrane By similarity

Amino acid modifications

Modified residue11N-acetylmethionine Ref.13 Ref.14 Ref.19

Experimental info

Sequence conflict281D → G in BAD97286. Ref.10

Sequences

Sequence LengthMass (Da)Tools
O75915 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: 3AA708C6D0901B44

FASTA18821,615
        10         20         30         40         50         60 
MDVNIAPLRA WDDFFPGSDR FARPDFRDIS KWNNRVVSNL LYYQTNYLVV AAMMISIVGF 

        70         80         90        100        110        120 
LSPFNMILGG IVVVLVFTGF VWAAHNKDVL RRMKKRYPTT FVMVVMLASY FLISMFGGVM 

       130        140        150        160        170        180 
VFVFGITFPL LLMFIHASLR LRNLKNKLEN KMEGIGLKRT PMGIVLDALE QQEEGINRLT 


DYISKVKE 

« Hide

References

« Hide 'large scale' references
[1]"Identification of addicsin/GTRAP3-18 as a chronic morphine-augumented gene in amygdala."
Ikemoto M.J., Inoue K., Akiduki S., Osugi T., Imamura T., Ishida N., Ohtomi M.
NeuroReport 13:2079-2084(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"JM4 is a four-transmembrane protein binding to the CCR5 receptor."
Schweneker M., Bachmann A.S., Moelling K.
FEBS Lett. 579:1751-1758(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Neuroblastoma.
[3]"Molecular cloning of a dermal papilla derived gene."
Ikeda A., Yoshimoto M.
Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Hair follicle dermal papilla.
[4]"A novel differentially displayed vitamin A responsive gene that is cytoskeleton related."
Zhou J.W., Di Y.P., Wu R.
Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[5]"Cloning of a novel human cDNA homologous to human mRNA for JM4 protein."
Zhang X.N., Yu L., Jin L., Hu P.R., Chen S.Y., Zhao S.Y.
Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[6]"A novel gene expressed in the human adrenal gland."
Jiang C., Huang C., Wu T., Peng Y., Gu Y., Zhang L., Zhang C., Li Y., Han Z., Wang Y., Chen Z., Fu G.
Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Adrenal gland.
[7]"Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells."
Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G., Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W., Tao J., Huang Q.-H., Zhou J., Hu G.-X. expand/collapse author list , Gu J., Chen S.-J., Chen Z.
Genome Res. 10:1546-1560(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Umbilical cord blood.
[8]"Large-scale identification and characterization of human genes that activate NF-kappaB and MAPK signaling pathways."
Matsuda A., Suzuki Y., Honda G., Muramatsu S., Matsuzaki O., Nagano Y., Doi T., Shimotohno K., Harada T., Nishida E., Hayashi H., Sugano S.
Oncogene 22:3307-3318(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Lung fibroblast.
[9]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[10]Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Spleen.
[11]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[12]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Bone marrow and Brain.
[13]"Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides."
Gevaert K., Goethals M., Martens L., Van Damme J., Staes A., Thomas G.R., Vandekerckhove J.
Nat. Biotechnol. 21:566-569(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-9, ACETYLATION AT MET-1.
Tissue: Platelet.
[14]Bienvenut W.V., Claeys D.
Submitted (NOV-2005) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 1-27; 152-158 AND 160-185, ACETYLATION AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY.
Tissue: Platelet.
[15]"Effect of differentiation inducer and heat stress on the expression of JWA protein and Hsp70 of K562 cells."
Mao W.G., Li A.P., Ye J., Huang S., Li A.Q., Zhou J.W.
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi 21:253-256(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[16]"Genomic organization, expression profile, and characterization of the new protein PRA1 domain family, member 2 (PRAF2)."
Fo C.S., Coleman C.S., Wallick C.J., Vine A.L., Bachmann A.S.
Gene 371:154-165(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NOMENCLATURE.
[17]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[18]"Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[19]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB052638 mRNA. Translation: BAD83603.1.
AY102608 mRNA. Translation: AAM28950.1.
AB014765 mRNA. Translation: BAC66462.1.
AF070523 mRNA. Translation: AAC64360.1.
AF115965 mRNA. Translation: AAP97237.1.
AF125530 mRNA. Translation: AAF17224.1.
AF161476 mRNA. Translation: AAF29091.1.
AB097051 mRNA. Translation: BAC77404.1.
AK223566 mRNA. Translation: BAD97286.1. Different initiation.
AK312731 mRNA. Translation: BAG35602.1.
CH471055 Genomic DNA. Translation: EAW65473.1.
BC005143 mRNA. Translation: AAH05143.1.
BC020797 mRNA. Translation: AAH20797.1.
CCDSCCDS2912.1.
RefSeqNP_006398.1. NM_006407.3.
UniGeneHs.518060.
Hs.745255.

3D structure databases

ProteinModelPortalO75915.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid115801. 13 interactions.
IntActO75915. 1 interaction.
MINTMINT-1339938.
STRING9606.ENSP00000273258.

PTM databases

PhosphoSiteO75915.

Proteomic databases

MaxQBO75915.
PaxDbO75915.
PeptideAtlasO75915.
PRIDEO75915.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000273258; ENSP00000273258; ENSG00000144746.
GeneID10550.
KEGGhsa:10550.
UCSCuc003dnr.3. human.

Organism-specific databases

CTD10550.
GeneCardsGC03P069216.
HGNCHGNC:16937. ARL6IP5.
HPACAB019321.
HPA015540.
MIM605709. gene.
neXtProtNX_O75915.
PharmGKBPA134898937.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG272388.
HOGENOMHOG000231363.
HOVERGENHBG053661.
InParanoidO75915.
OMAKMNKIQD.
PhylomeDBO75915.
TreeFamTF105479.

Gene expression databases

ArrayExpressO75915.
BgeeO75915.
CleanExHS_ARL6IP5.
GenevestigatorO75915.

Family and domain databases

InterProIPR004895. Prenylated_rab_accept_PRA1.
[Graphical view]
PfamPF03208. PRA1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSARL6IP5. human.
GeneWikiARL6IP5.
GenomeRNAi10550.
NextBio40017.
PROO75915.
SOURCESearch...

Entry information

Entry namePRAF3_HUMAN
AccessionPrimary (citable) accession number: O75915
Secondary accession number(s): B2R6V5, Q53ES3, Q5KU08
Entry history
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: November 1, 1998
Last modified: July 9, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 3

Human chromosome 3: entries, gene names and cross-references to MIM