Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

PRA1 family protein 3

Gene

ARL6IP5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulates intracellular concentrations of taurine and glutamate. Negatively modulates SLC1A1/EAAC1 glutamate transport activity by decreasing its affinity for glutamate in a PKC activity-dependent manner. May be involved in membrane traffic.By similarity

GO - Biological processi

  • intrinsic apoptotic signaling pathway in response to oxidative stress Source: UniProtKB
  • L-glutamate transport Source: UniProtKB
  • negative regulation of L-glutamate transport Source: UniProtKB
  • negative regulation of mitochondrial membrane potential Source: UniProtKB
  • positive regulation of apoptotic process Source: UniProtKB
  • positive regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
  • positive regulation of stress-activated MAPK cascade Source: UniProtKB
Complete GO annotation...

Enzyme and pathway databases

BioCyciZFISH:ENSG00000144746-MONOMER.
ReactomeiR-HSA-210500. Glutamate Neurotransmitter Release Cycle.

Protein family/group databases

TCDBi9.A.49.1.3. the preylated rab acceptor protein 1 (pra1) family.

Names & Taxonomyi

Protein namesi
Recommended name:
PRA1 family protein 3
Alternative name(s):
ADP-ribosylation factor-like protein 6-interacting protein 5
Short name:
ARL-6-interacting protein 5
Short name:
Aip-5
Cytoskeleton-related vitamin A-responsive protein
Dermal papilla-derived protein 11
GTRAP3-18
Glutamate transporter EAAC1-interacting protein
JM5
Prenylated Rab acceptor protein 2
Protein JWa
Putative MAPK-activating protein PM27
Gene namesi
Name:ARL6IP5
Synonyms:DERP11, JWA, PRA2, PRAF3
ORF Names:HSPC127
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:16937. ARL6IP5.

Subcellular locationi

  • Endoplasmic reticulum membrane By similarity; Multi-pass membrane protein Sequence analysis
  • Cell membrane By similarity; Multi-pass membrane protein Sequence analysis
  • Cytoplasm By similarity
  • Cytoplasmcytoskeleton By similarity

  • Note: Also exists as a soluble form in the cytoplasm. Associated with microtubules.By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 35CytoplasmicBy similarityAdd BLAST35
Transmembranei36 – 56HelicalSequence analysisAdd BLAST21
Transmembranei57 – 77HelicalSequence analysisAdd BLAST21
Topological domaini78 – 93CytoplasmicBy similarityAdd BLAST16
Transmembranei94 – 114HelicalSequence analysisAdd BLAST21
Transmembranei115 – 135HelicalSequence analysisAdd BLAST21
Topological domaini136 – 188CytoplasmicBy similarityAdd BLAST53

GO - Cellular componenti

  • cytoskeleton Source: UniProtKB-SubCell
  • endoplasmic reticulum Source: HPA
  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • extracellular exosome Source: UniProtKB
  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: UniProtKB
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoskeleton, Endoplasmic reticulum, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi10550.
OpenTargetsiENSG00000144746.
PharmGKBiPA134898937.

Polymorphism and mutation databases

BioMutaiARL6IP5.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002208831 – 188PRA1 family protein 3Add BLAST188

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources2 Publications1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiO75915.
MaxQBiO75915.
PaxDbiO75915.
PeptideAtlasiO75915.
PRIDEiO75915.
TopDownProteomicsiO75915.

PTM databases

iPTMnetiO75915.
PhosphoSitePlusiO75915.
SwissPalmiO75915.

Expressioni

Inductioni

By methyl-beta-cyclodextrin (By similarity). Up-regulated upon induced differentiation and in heat stress.By similarity1 Publication

Gene expression databases

BgeeiENSG00000144746.
CleanExiHS_ARL6IP5.
ExpressionAtlasiO75915. baseline and differential.
GenevisibleiO75915. HS.

Organism-specific databases

HPAiCAB019321.
HPA015540.

Interactioni

Subunit structurei

Homodimer. Heterodimer with ARL6IP1. Forms multimers. Interacts with prenylated RAB1A, RAB3A and SLC1A1/EAAC1. Does not interact with VAMP1, VAMP2 or VAMP3. Interacts with ARL6. Interacts with SLC1A1/EAAC1.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
RNF185Q96GF13EBI-2860752,EBI-2340249

Protein-protein interaction databases

BioGridi115801. 46 interactors.
IntActiO75915. 8 interactors.
MINTiMINT-1339938.
STRINGi9606.ENSP00000273258.

Structurei

3D structure databases

ProteinModelPortaliO75915.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni103 – 117Required for homodimer formation and heterodimer formation with ARL6IP1By similarityAdd BLAST15
Regioni136 – 188Targeting to endoplasmic reticulum membraneBy similarityAdd BLAST53

Sequence similaritiesi

Belongs to the PRA1 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4050. Eukaryota.
ENOG4111MUC. LUCA.
GeneTreeiENSGT00390000008631.
HOGENOMiHOG000231363.
HOVERGENiHBG053661.
InParanoidiO75915.
KOiK20393.
OMAiTNYLMVA.
OrthoDBiEOG091G0QS5.
PhylomeDBiO75915.
TreeFamiTF105479.

Family and domain databases

InterProiIPR004895. Prenylated_rab_accept_PRA1.
[Graphical view]
PfamiPF03208. PRA1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O75915-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDVNIAPLRA WDDFFPGSDR FARPDFRDIS KWNNRVVSNL LYYQTNYLVV
60 70 80 90 100
AAMMISIVGF LSPFNMILGG IVVVLVFTGF VWAAHNKDVL RRMKKRYPTT
110 120 130 140 150
FVMVVMLASY FLISMFGGVM VFVFGITFPL LLMFIHASLR LRNLKNKLEN
160 170 180
KMEGIGLKRT PMGIVLDALE QQEEGINRLT DYISKVKE
Length:188
Mass (Da):21,615
Last modified:November 1, 1998 - v1
Checksum:i3AA708C6D0901B44
GO

Sequence cautioni

The sequence BAD97286 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti28D → G in BAD97286 (Ref. 10) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB052638 mRNA. Translation: BAD83603.1.
AY102608 mRNA. Translation: AAM28950.1.
AB014765 mRNA. Translation: BAC66462.1.
AF070523 mRNA. Translation: AAC64360.1.
AF115965 mRNA. Translation: AAP97237.1.
AF125530 mRNA. Translation: AAF17224.1.
AF161476 mRNA. Translation: AAF29091.1.
AB097051 mRNA. Translation: BAC77404.1.
AK223566 mRNA. Translation: BAD97286.1. Different initiation.
AK312731 mRNA. Translation: BAG35602.1.
CH471055 Genomic DNA. Translation: EAW65473.1.
BC005143 mRNA. Translation: AAH05143.1.
BC020797 mRNA. Translation: AAH20797.1.
CCDSiCCDS2912.1.
RefSeqiNP_006398.1. NM_006407.3.
UniGeneiHs.518060.
Hs.745255.

Genome annotation databases

EnsembliENST00000273258; ENSP00000273258; ENSG00000144746.
GeneIDi10550.
KEGGihsa:10550.
UCSCiuc003dnr.4. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB052638 mRNA. Translation: BAD83603.1.
AY102608 mRNA. Translation: AAM28950.1.
AB014765 mRNA. Translation: BAC66462.1.
AF070523 mRNA. Translation: AAC64360.1.
AF115965 mRNA. Translation: AAP97237.1.
AF125530 mRNA. Translation: AAF17224.1.
AF161476 mRNA. Translation: AAF29091.1.
AB097051 mRNA. Translation: BAC77404.1.
AK223566 mRNA. Translation: BAD97286.1. Different initiation.
AK312731 mRNA. Translation: BAG35602.1.
CH471055 Genomic DNA. Translation: EAW65473.1.
BC005143 mRNA. Translation: AAH05143.1.
BC020797 mRNA. Translation: AAH20797.1.
CCDSiCCDS2912.1.
RefSeqiNP_006398.1. NM_006407.3.
UniGeneiHs.518060.
Hs.745255.

3D structure databases

ProteinModelPortaliO75915.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115801. 46 interactors.
IntActiO75915. 8 interactors.
MINTiMINT-1339938.
STRINGi9606.ENSP00000273258.

Protein family/group databases

TCDBi9.A.49.1.3. the preylated rab acceptor protein 1 (pra1) family.

PTM databases

iPTMnetiO75915.
PhosphoSitePlusiO75915.
SwissPalmiO75915.

Polymorphism and mutation databases

BioMutaiARL6IP5.

Proteomic databases

EPDiO75915.
MaxQBiO75915.
PaxDbiO75915.
PeptideAtlasiO75915.
PRIDEiO75915.
TopDownProteomicsiO75915.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000273258; ENSP00000273258; ENSG00000144746.
GeneIDi10550.
KEGGihsa:10550.
UCSCiuc003dnr.4. human.

Organism-specific databases

CTDi10550.
DisGeNETi10550.
GeneCardsiARL6IP5.
HGNCiHGNC:16937. ARL6IP5.
HPAiCAB019321.
HPA015540.
MIMi605709. gene.
neXtProtiNX_O75915.
OpenTargetsiENSG00000144746.
PharmGKBiPA134898937.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4050. Eukaryota.
ENOG4111MUC. LUCA.
GeneTreeiENSGT00390000008631.
HOGENOMiHOG000231363.
HOVERGENiHBG053661.
InParanoidiO75915.
KOiK20393.
OMAiTNYLMVA.
OrthoDBiEOG091G0QS5.
PhylomeDBiO75915.
TreeFamiTF105479.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000144746-MONOMER.
ReactomeiR-HSA-210500. Glutamate Neurotransmitter Release Cycle.

Miscellaneous databases

ChiTaRSiARL6IP5. human.
GeneWikiiARL6IP5.
GenomeRNAii10550.
PROiO75915.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000144746.
CleanExiHS_ARL6IP5.
ExpressionAtlasiO75915. baseline and differential.
GenevisibleiO75915. HS.

Family and domain databases

InterProiIPR004895. Prenylated_rab_accept_PRA1.
[Graphical view]
PfamiPF03208. PRA1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPRAF3_HUMAN
AccessioniPrimary (citable) accession number: O75915
Secondary accession number(s): B2R6V5, Q53ES3, Q5KU08
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: November 1, 1998
Last modified: November 30, 2016
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.