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Protein

PRA1 family protein 3

Gene

ARL6IP5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulates intracellular concentrations of taurine and glutamate. Negatively modulates SLC1A1/EAAC1 glutamate transport activity by decreasing its affinity for glutamate. May be involved in membrane traffic (By similarity).By similarity

GO - Biological processi

  • intrinsic apoptotic signaling pathway in response to oxidative stress Source: UniProtKB
  • L-glutamate transport Source: UniProtKB
  • negative regulation of mitochondrial membrane potential Source: UniProtKB
  • negative regulation of transport Source: Ensembl
  • positive regulation of apoptotic process Source: UniProtKB
  • positive regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
  • positive regulation of stress-activated MAPK cascade Source: UniProtKB
Complete GO annotation...

Enzyme and pathway databases

ReactomeiREACT_12591. Glutamate Neurotransmitter Release Cycle.

Names & Taxonomyi

Protein namesi
Recommended name:
PRA1 family protein 3
Alternative name(s):
ADP-ribosylation factor-like protein 6-interacting protein 5
Short name:
ARL-6-interacting protein 5
Short name:
Aip-5
Cytoskeleton-related vitamin A-responsive protein
Dermal papilla-derived protein 11
GTRAP3-18
Glutamate transporter EAAC1-interacting protein
JM5
Prenylated Rab acceptor protein 2
Protein JWa
Putative MAPK-activating protein PM27
Gene namesi
Name:ARL6IP5
Synonyms:DERP11, JWA, PRA2, PRAF3
ORF Names:HSPC127
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:16937. ARL6IP5.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3535CytoplasmicBy similarityAdd
BLAST
Transmembranei36 – 5621HelicalSequence AnalysisAdd
BLAST
Transmembranei57 – 7721HelicalSequence AnalysisAdd
BLAST
Topological domaini78 – 9316CytoplasmicBy similarityAdd
BLAST
Transmembranei94 – 11421HelicalSequence AnalysisAdd
BLAST
Transmembranei115 – 13521HelicalSequence AnalysisAdd
BLAST
Topological domaini136 – 18853CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  • endoplasmic reticulum Source: HPA
  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • extracellular exosome Source: UniProtKB
  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134898937.

Polymorphism and mutation databases

BioMutaiARL6IP5.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 188188PRA1 family protein 3PRO_0000220883Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine3 Publications

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiO75915.
PaxDbiO75915.
PeptideAtlasiO75915.
PRIDEiO75915.

PTM databases

PhosphoSiteiO75915.

Expressioni

Inductioni

By methyl-beta-cyclodextrin (By similarity). Up-regulated upon induced differentiation and in heat stress.By similarity1 Publication

Gene expression databases

BgeeiO75915.
CleanExiHS_ARL6IP5.
ExpressionAtlasiO75915. baseline and differential.
GenevisibleiO75915. HS.

Organism-specific databases

HPAiCAB019321.
HPA015540.

Interactioni

Subunit structurei

Forms multimers. Binds to prenylated RAB1A and RAB3A, to the neuronal glutamate transporter SLC1A1/EAAC1, and to the ADP-ribosylation like factor ARL6, a member of the Ras superfamily. Does not bind to VAMP2 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
RNF185Q96GF13EBI-2860752,EBI-2340249

Protein-protein interaction databases

BioGridi115801. 24 interactions.
IntActiO75915. 2 interactions.
MINTiMINT-1339938.
STRINGi9606.ENSP00000273258.

Structurei

3D structure databases

ProteinModelPortaliO75915.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni136 – 18853Targeting to endoplasmic reticulum membraneBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the PRA1 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG272388.
GeneTreeiENSGT00390000008631.
HOGENOMiHOG000231363.
HOVERGENiHBG053661.
InParanoidiO75915.
OMAiNYLMVAA.
PhylomeDBiO75915.
TreeFamiTF105479.

Family and domain databases

InterProiIPR004895. Prenylated_rab_accept_PRA1.
[Graphical view]
PfamiPF03208. PRA1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O75915-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDVNIAPLRA WDDFFPGSDR FARPDFRDIS KWNNRVVSNL LYYQTNYLVV
60 70 80 90 100
AAMMISIVGF LSPFNMILGG IVVVLVFTGF VWAAHNKDVL RRMKKRYPTT
110 120 130 140 150
FVMVVMLASY FLISMFGGVM VFVFGITFPL LLMFIHASLR LRNLKNKLEN
160 170 180
KMEGIGLKRT PMGIVLDALE QQEEGINRLT DYISKVKE
Length:188
Mass (Da):21,615
Last modified:November 1, 1998 - v1
Checksum:i3AA708C6D0901B44
GO

Sequence cautioni

The sequence BAD97286.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti28 – 281D → G in BAD97286 (Ref. 10) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB052638 mRNA. Translation: BAD83603.1.
AY102608 mRNA. Translation: AAM28950.1.
AB014765 mRNA. Translation: BAC66462.1.
AF070523 mRNA. Translation: AAC64360.1.
AF115965 mRNA. Translation: AAP97237.1.
AF125530 mRNA. Translation: AAF17224.1.
AF161476 mRNA. Translation: AAF29091.1.
AB097051 mRNA. Translation: BAC77404.1.
AK223566 mRNA. Translation: BAD97286.1. Different initiation.
AK312731 mRNA. Translation: BAG35602.1.
CH471055 Genomic DNA. Translation: EAW65473.1.
BC005143 mRNA. Translation: AAH05143.1.
BC020797 mRNA. Translation: AAH20797.1.
CCDSiCCDS2912.1.
RefSeqiNP_006398.1. NM_006407.3.
UniGeneiHs.518060.
Hs.745255.

Genome annotation databases

EnsembliENST00000273258; ENSP00000273258; ENSG00000144746.
GeneIDi10550.
KEGGihsa:10550.
UCSCiuc003dnr.3. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB052638 mRNA. Translation: BAD83603.1.
AY102608 mRNA. Translation: AAM28950.1.
AB014765 mRNA. Translation: BAC66462.1.
AF070523 mRNA. Translation: AAC64360.1.
AF115965 mRNA. Translation: AAP97237.1.
AF125530 mRNA. Translation: AAF17224.1.
AF161476 mRNA. Translation: AAF29091.1.
AB097051 mRNA. Translation: BAC77404.1.
AK223566 mRNA. Translation: BAD97286.1. Different initiation.
AK312731 mRNA. Translation: BAG35602.1.
CH471055 Genomic DNA. Translation: EAW65473.1.
BC005143 mRNA. Translation: AAH05143.1.
BC020797 mRNA. Translation: AAH20797.1.
CCDSiCCDS2912.1.
RefSeqiNP_006398.1. NM_006407.3.
UniGeneiHs.518060.
Hs.745255.

3D structure databases

ProteinModelPortaliO75915.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115801. 24 interactions.
IntActiO75915. 2 interactions.
MINTiMINT-1339938.
STRINGi9606.ENSP00000273258.

PTM databases

PhosphoSiteiO75915.

Polymorphism and mutation databases

BioMutaiARL6IP5.

Proteomic databases

MaxQBiO75915.
PaxDbiO75915.
PeptideAtlasiO75915.
PRIDEiO75915.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000273258; ENSP00000273258; ENSG00000144746.
GeneIDi10550.
KEGGihsa:10550.
UCSCiuc003dnr.3. human.

Organism-specific databases

CTDi10550.
GeneCardsiGC03P069216.
HGNCiHGNC:16937. ARL6IP5.
HPAiCAB019321.
HPA015540.
MIMi605709. gene.
neXtProtiNX_O75915.
PharmGKBiPA134898937.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG272388.
GeneTreeiENSGT00390000008631.
HOGENOMiHOG000231363.
HOVERGENiHBG053661.
InParanoidiO75915.
OMAiNYLMVAA.
PhylomeDBiO75915.
TreeFamiTF105479.

Enzyme and pathway databases

ReactomeiREACT_12591. Glutamate Neurotransmitter Release Cycle.

Miscellaneous databases

ChiTaRSiARL6IP5. human.
GeneWikiiARL6IP5.
GenomeRNAii10550.
NextBioi40017.
PROiO75915.
SOURCEiSearch...

Gene expression databases

BgeeiO75915.
CleanExiHS_ARL6IP5.
ExpressionAtlasiO75915. baseline and differential.
GenevisibleiO75915. HS.

Family and domain databases

InterProiIPR004895. Prenylated_rab_accept_PRA1.
[Graphical view]
PfamiPF03208. PRA1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of addicsin/GTRAP3-18 as a chronic morphine-augumented gene in amygdala."
    Ikemoto M.J., Inoue K., Akiduki S., Osugi T., Imamura T., Ishida N., Ohtomi M.
    NeuroReport 13:2079-2084(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "JM4 is a four-transmembrane protein binding to the CCR5 receptor."
    Schweneker M., Bachmann A.S., Moelling K.
    FEBS Lett. 579:1751-1758(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Neuroblastoma.
  3. "Molecular cloning of a dermal papilla derived gene."
    Ikeda A., Yoshimoto M.
    Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Hair follicle dermal papilla.
  4. "A novel differentially displayed vitamin A responsive gene that is cytoskeleton related."
    Zhou J.W., Di Y.P., Wu R.
    Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  5. "Cloning of a novel human cDNA homologous to human mRNA for JM4 protein."
    Zhang X.N., Yu L., Jin L., Hu P.R., Chen S.Y., Zhao S.Y.
    Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  6. "A novel gene expressed in the human adrenal gland."
    Jiang C., Huang C., Wu T., Peng Y., Gu Y., Zhang L., Zhang C., Li Y., Han Z., Wang Y., Chen Z., Fu G.
    Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Adrenal gland.
  7. "Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells."
    Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G., Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W., Tao J., Huang Q.-H., Zhou J., Hu G.-X.
    , Gu J., Chen S.-J., Chen Z.
    Genome Res. 10:1546-1560(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Umbilical cord blood.
  8. "Large-scale identification and characterization of human genes that activate NF-kappaB and MAPK signaling pathways."
    Matsuda A., Suzuki Y., Honda G., Muramatsu S., Matsuzaki O., Nagano Y., Doi T., Shimotohno K., Harada T., Nishida E., Hayashi H., Sugano S.
    Oncogene 22:3307-3318(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung fibroblast.
  9. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  10. Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
    Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Spleen.
  11. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  12. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Bone marrow and Brain.
  13. "Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides."
    Gevaert K., Goethals M., Martens L., Van Damme J., Staes A., Thomas G.R., Vandekerckhove J.
    Nat. Biotechnol. 21:566-569(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-9, ACETYLATION AT MET-1.
    Tissue: Platelet.
  14. Bienvenut W.V., Claeys D.
    Submitted (NOV-2005) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 1-27; 152-158 AND 160-185, ACETYLATION AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Platelet.
  15. "Effect of differentiation inducer and heat stress on the expression of JWA protein and Hsp70 of K562 cells."
    Mao W.G., Li A.P., Ye J., Huang S., Li A.Q., Zhou J.W.
    Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi 21:253-256(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  16. "Genomic organization, expression profile, and characterization of the new protein PRA1 domain family, member 2 (PRAF2)."
    Fo C.S., Coleman C.S., Wallick C.J., Vine A.L., Bachmann A.S.
    Gene 371:154-165(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE.
  17. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  18. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
    Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
    Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  19. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  20. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiPRAF3_HUMAN
AccessioniPrimary (citable) accession number: O75915
Secondary accession number(s): B2R6V5, Q53ES3, Q5KU08
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: November 1, 1998
Last modified: June 24, 2015
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.