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O75912

- DGKI_HUMAN

UniProt

O75912 - DGKI_HUMAN

Protein

Diacylglycerol kinase iota

Gene

DGKI

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 131 (01 Oct 2014)
      Sequence version 1 (01 Nov 1998)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri178 – 23255Phorbol-ester/DAG-type 1Add
    BLAST
    Zinc fingeri251 – 30959Phorbol-ester/DAG-type 2Add
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. diacylglycerol kinase activity Source: ProtInc
    3. GTPase inhibitor activity Source: Ensembl
    4. metal ion binding Source: UniProtKB-KW
    5. NAD+ kinase activity Source: InterPro
    6. protein binding Source: IntAct

    GO - Biological processi

    1. blood coagulation Source: Reactome
    2. intracellular signal transduction Source: InterPro
    3. platelet activation Source: Reactome
    4. positive regulation of Ras protein signal transduction Source: Ensembl
    5. protein kinase C-activating G-protein coupled receptor signaling pathway Source: InterPro
    6. regulation of Rap GTPase activity Source: Ensembl

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_2202. Effects of PIP2 hydrolysis.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Diacylglycerol kinase iota (EC:2.7.1.107)
    Short name:
    DAG kinase iota
    Alternative name(s):
    Diglyceride kinase iota
    Short name:
    DGK-iota
    Gene namesi
    Name:DGKI
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 7

    Organism-specific databases

    HGNCiHGNC:2855. DGKI.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: ProtInc
    2. guanyl-nucleotide exchange factor complex Source: Ensembl
    3. nucleus Source: HPA
    4. perinuclear region of cytoplasm Source: Ensembl
    5. plasma membrane Source: Reactome

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA27316.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10651065Diacylglycerol kinase iotaPRO_0000218466Add
    BLAST

    Proteomic databases

    PaxDbiO75912.
    PRIDEiO75912.

    PTM databases

    PhosphoSiteiO75912.

    Expressioni

    Gene expression databases

    ArrayExpressiO75912.
    BgeeiO75912.
    CleanExiHS_DGKI.
    GenevestigatoriO75912.

    Organism-specific databases

    HPAiHPA021924.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    VHLP40337-33EBI-1765520,EBI-301270

    Protein-protein interaction databases

    BioGridi114605. 3 interactions.
    IntActiO75912. 1 interaction.
    MINTiMINT-4657619.
    STRINGi9606.ENSP00000288490.

    Structurei

    3D structure databases

    ProteinModelPortaliO75912.
    SMRiO75912. Positions 938-1050.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini372 – 507136DAGKcPROSITE-ProRule annotationAdd
    BLAST
    Repeati958 – 99033ANK 1Add
    BLAST
    Repeati997 – 102630ANK 2Add
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi20 – 3112Poly-AlaAdd
    BLAST
    Compositional biasi69 – 746Poly-Ser
    Compositional biasi95 – 1028Poly-Ala

    Sequence similaritiesi

    Contains 2 ANK repeats.PROSITE-ProRule annotation
    Contains 1 DAGKc domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri178 – 23255Phorbol-ester/DAG-type 1Add
    BLAST
    Zinc fingeri251 – 30959Phorbol-ester/DAG-type 2Add
    BLAST

    Keywords - Domaini

    ANK repeat, Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiNOG47311.
    HOGENOMiHOG000231472.
    HOVERGENiHBG067303.
    InParanoidiO75912.
    KOiK00901.
    PhylomeDBiO75912.
    TreeFamiTF312817.

    Family and domain databases

    Gene3Di1.25.40.20. 1 hit.
    InterProiIPR002110. Ankyrin_rpt.
    IPR020683. Ankyrin_rpt-contain_dom.
    IPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR000756. Diacylglycerol_kin_accessory.
    IPR001206. Diacylglycerol_kinase_cat_dom.
    IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
    [Graphical view]
    PfamiPF00023. Ank. 2 hits.
    PF00130. C1_1. 1 hit.
    PF00609. DAGK_acc. 1 hit.
    PF00781. DAGK_cat. 1 hit.
    [Graphical view]
    SMARTiSM00248. ANK. 2 hits.
    SM00109. C1. 2 hits.
    SM00045. DAGKa. 1 hit.
    SM00046. DAGKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF111331. SSF111331. 1 hit.
    SSF48403. SSF48403. 1 hit.
    PROSITEiPS50297. ANK_REP_REGION. 1 hit.
    PS50088. ANK_REPEAT. 2 hits.
    PS50146. DAGK. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O75912-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDAAGRGCHL LPLPAARGPA RAPAAAAAAA ASPPGPCSGA ACAPSAAAGA     50
    GAMNPSSSAG EEKGATGGSS SSGSGAGSCC LGAEGGADPR GAGSAAAAGA 100
    AALDEPAAAG QKEKDEALEE KLRNLTFRKQ VSYRKAISRA GLQHLAPAHP 150
    LSLPVANGPA KEPRATLDWS ENAVNGEHLW LETNVSGDLC YLGEENCQVR 200
    FAKSALRRKC AVCKIVVHTA CIEQLEKINF RCKPTFREGG SRSPRENFVR 250
    HHWVHRRRQE GKCKQCGKGF QQKFSFHSKE IVAISCSWCK QAFHNKVTCF 300
    MLHHIEEPCS LGAHAAVIVP PTWIIKVKKP QNSLKASNRK KKRTSFKRKA 350
    SKRGMEQENK GRPFVIKPIS SPLMKPLLVF VNPKSGGNQG TKVLQMFMWY 400
    LNPRQVFDLS QEGPKDALEL YRKVPNLRIL ACGGDGTVGW ILSILDELQL 450
    SPQPPVGVLP LGTGNDLART LNWGGGYTDE PVSKILCQVE DGTVVQLDRW 500
    NLHVERNPDL PPEELEDGVC KLPLNVFNNY FSLGFDAHVT LEFHESREAN 550
    PEKFNSRFRN KMFYAGAAFS DFLQRSSRDL SKHVKVVCDG TDLTPKIQEL 600
    KFQCIVFLNI PRYCAGTMPW GNPGDHHDFE PQRHDDGYIE VIGFTMASLA 650
    ALQVGGHGER LHQCREVMLL TYKSIPMQVD GEPCRLAPAM IRISLRNQAN 700
    MVQKSKRRTS MPLLNDPQSV PDRLRIRVNK ISLQDYEGFH YDKEKLREAS 750
    ISDWLRTIAG ELVQSFGAIP LGILVVRGDC DLETCRMYID RLQEDLQSVS 800
    SGSQRVHYQD HETSFPRALS AQRLSPRWCF LDDRSQEHLH FVMEISQDEI 850
    FILDPDMVVS QPAGTPPGMP DLVVEQASGI SDWWNPALRK RMLSDSGLGM 900
    IAPYYEDSDL KDLSHSRVLQ SPVSSEDHAI LQAVIAGDLM KLIESYKNGG 950
    SLLIQGPDHC SLLHYAAKTG NGEIVKYILD HGPSELLDMA DSETGETALH 1000
    KAACQRNRAV CQLLVDAGAS LRKTDSKGKT PQERAQQAGD PDLAAYLESR 1050
    QNYKVIGHED LETAV 1065
    Length:1,065
    Mass (Da):116,997
    Last modified:November 1, 1998 - v1
    Checksum:iB84971AA7630A799
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti160 – 1601A → P in AAF43006. (PubMed:10706894)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti153 – 1531L → F.1 Publication
    Corresponds to variant rs61757580 [ dbSNP | Ensembl ].
    VAR_010190

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF061936 mRNA. Translation: AAC62010.1.
    CH236950 Genomic DNA. Translation: EAL24051.1.
    AH009185 Genomic DNA. Translation: AAF43006.1.
    CCDSiCCDS5845.1.
    RefSeqiNP_004708.1. NM_004717.2.
    UniGeneiHs.242947.

    Genome annotation databases

    EnsembliENST00000288490; ENSP00000288490; ENSG00000157680.
    GeneIDi9162.
    KEGGihsa:9162.
    UCSCiuc003vtt.3. human.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF061936 mRNA. Translation: AAC62010.1 .
    CH236950 Genomic DNA. Translation: EAL24051.1 .
    AH009185 Genomic DNA. Translation: AAF43006.1 .
    CCDSi CCDS5845.1.
    RefSeqi NP_004708.1. NM_004717.2.
    UniGenei Hs.242947.

    3D structure databases

    ProteinModelPortali O75912.
    SMRi O75912. Positions 938-1050.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 114605. 3 interactions.
    IntActi O75912. 1 interaction.
    MINTi MINT-4657619.
    STRINGi 9606.ENSP00000288490.

    PTM databases

    PhosphoSitei O75912.

    Proteomic databases

    PaxDbi O75912.
    PRIDEi O75912.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000288490 ; ENSP00000288490 ; ENSG00000157680 .
    GeneIDi 9162.
    KEGGi hsa:9162.
    UCSCi uc003vtt.3. human.

    Organism-specific databases

    CTDi 9162.
    GeneCardsi GC07M137073.
    HGNCi HGNC:2855. DGKI.
    HPAi HPA021924.
    MIMi 604072. gene.
    neXtProti NX_O75912.
    PharmGKBi PA27316.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG47311.
    HOGENOMi HOG000231472.
    HOVERGENi HBG067303.
    InParanoidi O75912.
    KOi K00901.
    PhylomeDBi O75912.
    TreeFami TF312817.

    Enzyme and pathway databases

    Reactomei REACT_2202. Effects of PIP2 hydrolysis.

    Miscellaneous databases

    GenomeRNAii 9162.
    NextBioi 34367.
    PROi O75912.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O75912.
    Bgeei O75912.
    CleanExi HS_DGKI.
    Genevestigatori O75912.

    Family and domain databases

    Gene3Di 1.25.40.20. 1 hit.
    InterProi IPR002110. Ankyrin_rpt.
    IPR020683. Ankyrin_rpt-contain_dom.
    IPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR000756. Diacylglycerol_kin_accessory.
    IPR001206. Diacylglycerol_kinase_cat_dom.
    IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
    [Graphical view ]
    Pfami PF00023. Ank. 2 hits.
    PF00130. C1_1. 1 hit.
    PF00609. DAGK_acc. 1 hit.
    PF00781. DAGK_cat. 1 hit.
    [Graphical view ]
    SMARTi SM00248. ANK. 2 hits.
    SM00109. C1. 2 hits.
    SM00045. DAGKa. 1 hit.
    SM00046. DAGKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF111331. SSF111331. 1 hit.
    SSF48403. SSF48403. 1 hit.
    PROSITEi PS50297. ANK_REP_REGION. 1 hit.
    PS50088. ANK_REPEAT. 2 hits.
    PS50146. DAGK. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The cloning and characterization of a novel human diacylglycerol kinase, DGK-iota."
      Ding L., Traer E., McIntyre T.M., Zimmerman G.A., Prescott S.M.
      J. Biol. Chem. 273:32746-32752(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Retina.
    2. "Human chromosome 7: DNA sequence and biology."
      Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K., Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R., Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A., Kanematsu E., Gentles S.
      , Christopoulos C.C., Choufani S., Kwasnicka D., Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z., Lu F., Zeesman S., Nowaczyk M.J., Teshima I., Chitayat D., Shuman C., Weksberg R., Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J., Rahman N., Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F., Belloni E., Shaffer L.G., Pober B., Morton C.C., Gusella J.F., Bruns G.A.P., Korf B.R., Quade B.J., Ligon A.H., Ferguson H., Higgins A.W., Leach N.T., Herrick S.R., Lemyre E., Farra C.G., Kim H.-G., Summers A.M., Gripp K.W., Roberts W., Szatmari P., Winsor E.J.T., Grzeschik K.-H., Teebi A., Minassian B.A., Kere J., Armengol L., Pujana M.A., Estivill X., Wilson M.D., Koop B.F., Tosi S., Moore G.E., Boright A.P., Zlotorynski E., Kerem B., Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H., Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W., Mural R.J., Adams M.D., Tsui L.-C.
      Science 300:767-772(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "Evaluation of human diacylglycerol kinase iota, DGKI, a homolog of Drosophila rdgA, in inherited retinopathy mapping to 7q."
      Bowne S.J., Sullivan L.S., Ding L., Traer E., Prescott S.M., Birch D.G., Kennan A., Humphries P., Daiger S.P.
      Mol. Vis. 6:6-9(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 135-1065, VARIANT PHE-153.

    Entry informationi

    Entry nameiDGKI_HUMAN
    AccessioniPrimary (citable) accession number: O75912
    Secondary accession number(s): A4D1Q9, Q9NZ49
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 11, 2001
    Last sequence update: November 1, 1998
    Last modified: October 1, 2014
    This is version 131 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 7
      Human chromosome 7: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3