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O75912 (DGKI_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 127. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Diacylglycerol kinase iota

Short name=DAG kinase iota
EC=2.7.1.107
Alternative name(s):
Diglyceride kinase iota
Short name=DGK-iota
Gene names
Name:DGKI
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1065 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

Subcellular location

Cytoplasm. Nucleus.

Sequence similarities

Belongs to the eukaryotic diacylglycerol kinase family.

Contains 2 ANK repeats.

Contains 1 DAGKc domain.

Contains 2 phorbol-ester/DAG-type zinc fingers.

Ontologies

Keywords
   Cellular componentCytoplasm
Nucleus
   Coding sequence diversityPolymorphism
   DomainANK repeat
Repeat
Zinc-finger
   LigandATP-binding
Metal-binding
Nucleotide-binding
Zinc
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processblood coagulation

Traceable author statement. Source: Reactome

intracellular signal transduction

Inferred from electronic annotation. Source: InterPro

platelet activation

Traceable author statement. Source: Reactome

positive regulation of Ras protein signal transduction

Inferred from electronic annotation. Source: Ensembl

protein kinase C-activating G-protein coupled receptor signaling pathway

Inferred from electronic annotation. Source: InterPro

regulation of Rap GTPase activity

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentcytoplasm

Traceable author statement Ref.1. Source: ProtInc

guanyl-nucleotide exchange factor complex

Inferred from electronic annotation. Source: Ensembl

nucleus

Inferred from direct assay. Source: HPA

perinuclear region of cytoplasm

Inferred from electronic annotation. Source: Ensembl

plasma membrane

Traceable author statement. Source: Reactome

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

GTPase inhibitor activity

Inferred from electronic annotation. Source: Ensembl

NAD+ kinase activity

Inferred from electronic annotation. Source: InterPro

diacylglycerol kinase activity

Traceable author statement Ref.1. Source: ProtInc

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

VHLP40337-33EBI-1765520,EBI-301270

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10651065Diacylglycerol kinase iota
PRO_0000218466

Regions

Domain372 – 507136DAGKc
Repeat958 – 99033ANK 1
Repeat997 – 102630ANK 2
Zinc finger178 – 23255Phorbol-ester/DAG-type 1
Zinc finger251 – 30959Phorbol-ester/DAG-type 2
Compositional bias20 – 3112Poly-Ala
Compositional bias69 – 746Poly-Ser
Compositional bias95 – 1028Poly-Ala

Natural variations

Natural variant1531L → F. Ref.3
Corresponds to variant rs61757580 [ dbSNP | Ensembl ].
VAR_010190

Experimental info

Sequence conflict1601A → P in AAF43006. Ref.3

Sequences

Sequence LengthMass (Da)Tools
O75912 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: B84971AA7630A799

FASTA1,065116,997
        10         20         30         40         50         60 
MDAAGRGCHL LPLPAARGPA RAPAAAAAAA ASPPGPCSGA ACAPSAAAGA GAMNPSSSAG 

        70         80         90        100        110        120 
EEKGATGGSS SSGSGAGSCC LGAEGGADPR GAGSAAAAGA AALDEPAAAG QKEKDEALEE 

       130        140        150        160        170        180 
KLRNLTFRKQ VSYRKAISRA GLQHLAPAHP LSLPVANGPA KEPRATLDWS ENAVNGEHLW 

       190        200        210        220        230        240 
LETNVSGDLC YLGEENCQVR FAKSALRRKC AVCKIVVHTA CIEQLEKINF RCKPTFREGG 

       250        260        270        280        290        300 
SRSPRENFVR HHWVHRRRQE GKCKQCGKGF QQKFSFHSKE IVAISCSWCK QAFHNKVTCF 

       310        320        330        340        350        360 
MLHHIEEPCS LGAHAAVIVP PTWIIKVKKP QNSLKASNRK KKRTSFKRKA SKRGMEQENK 

       370        380        390        400        410        420 
GRPFVIKPIS SPLMKPLLVF VNPKSGGNQG TKVLQMFMWY LNPRQVFDLS QEGPKDALEL 

       430        440        450        460        470        480 
YRKVPNLRIL ACGGDGTVGW ILSILDELQL SPQPPVGVLP LGTGNDLART LNWGGGYTDE 

       490        500        510        520        530        540 
PVSKILCQVE DGTVVQLDRW NLHVERNPDL PPEELEDGVC KLPLNVFNNY FSLGFDAHVT 

       550        560        570        580        590        600 
LEFHESREAN PEKFNSRFRN KMFYAGAAFS DFLQRSSRDL SKHVKVVCDG TDLTPKIQEL 

       610        620        630        640        650        660 
KFQCIVFLNI PRYCAGTMPW GNPGDHHDFE PQRHDDGYIE VIGFTMASLA ALQVGGHGER 

       670        680        690        700        710        720 
LHQCREVMLL TYKSIPMQVD GEPCRLAPAM IRISLRNQAN MVQKSKRRTS MPLLNDPQSV 

       730        740        750        760        770        780 
PDRLRIRVNK ISLQDYEGFH YDKEKLREAS ISDWLRTIAG ELVQSFGAIP LGILVVRGDC 

       790        800        810        820        830        840 
DLETCRMYID RLQEDLQSVS SGSQRVHYQD HETSFPRALS AQRLSPRWCF LDDRSQEHLH 

       850        860        870        880        890        900 
FVMEISQDEI FILDPDMVVS QPAGTPPGMP DLVVEQASGI SDWWNPALRK RMLSDSGLGM 

       910        920        930        940        950        960 
IAPYYEDSDL KDLSHSRVLQ SPVSSEDHAI LQAVIAGDLM KLIESYKNGG SLLIQGPDHC 

       970        980        990       1000       1010       1020 
SLLHYAAKTG NGEIVKYILD HGPSELLDMA DSETGETALH KAACQRNRAV CQLLVDAGAS 

      1030       1040       1050       1060 
LRKTDSKGKT PQERAQQAGD PDLAAYLESR QNYKVIGHED LETAV 

« Hide

References

« Hide 'large scale' references
[1]"The cloning and characterization of a novel human diacylglycerol kinase, DGK-iota."
Ding L., Traer E., McIntyre T.M., Zimmerman G.A., Prescott S.M.
J. Biol. Chem. 273:32746-32752(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Retina.
[2]"Human chromosome 7: DNA sequence and biology."
Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K., Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R., Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A., Kanematsu E., Gentles S. expand/collapse author list , Christopoulos C.C., Choufani S., Kwasnicka D., Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z., Lu F., Zeesman S., Nowaczyk M.J., Teshima I., Chitayat D., Shuman C., Weksberg R., Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J., Rahman N., Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F., Belloni E., Shaffer L.G., Pober B., Morton C.C., Gusella J.F., Bruns G.A.P., Korf B.R., Quade B.J., Ligon A.H., Ferguson H., Higgins A.W., Leach N.T., Herrick S.R., Lemyre E., Farra C.G., Kim H.-G., Summers A.M., Gripp K.W., Roberts W., Szatmari P., Winsor E.J.T., Grzeschik K.-H., Teebi A., Minassian B.A., Kere J., Armengol L., Pujana M.A., Estivill X., Wilson M.D., Koop B.F., Tosi S., Moore G.E., Boright A.P., Zlotorynski E., Kerem B., Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H., Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W., Mural R.J., Adams M.D., Tsui L.-C.
Science 300:767-772(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"Evaluation of human diacylglycerol kinase iota, DGKI, a homolog of Drosophila rdgA, in inherited retinopathy mapping to 7q."
Bowne S.J., Sullivan L.S., Ding L., Traer E., Prescott S.M., Birch D.G., Kennan A., Humphries P., Daiger S.P.
Mol. Vis. 6:6-9(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 135-1065, VARIANT PHE-153.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF061936 mRNA. Translation: AAC62010.1.
CH236950 Genomic DNA. Translation: EAL24051.1.
AH009185 Genomic DNA. Translation: AAF43006.1.
RefSeqNP_004708.1. NM_004717.2.
UniGeneHs.242947.

3D structure databases

ProteinModelPortalO75912.
SMRO75912. Positions 938-1050.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid114605. 3 interactions.
IntActO75912. 1 interaction.
MINTMINT-4657619.
STRING9606.ENSP00000288490.

PTM databases

PhosphoSiteO75912.

Proteomic databases

PaxDbO75912.
PRIDEO75912.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000288490; ENSP00000288490; ENSG00000157680.
GeneID9162.
KEGGhsa:9162.
UCSCuc003vtt.3. human.

Organism-specific databases

CTD9162.
GeneCardsGC07M137073.
HGNCHGNC:2855. DGKI.
HPAHPA021924.
MIM604072. gene.
neXtProtNX_O75912.
PharmGKBPA27316.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG47311.
HOGENOMHOG000231472.
HOVERGENHBG067303.
InParanoidO75912.
KOK00901.
PhylomeDBO75912.
TreeFamTF312817.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.
REACT_604. Hemostasis.

Gene expression databases

ArrayExpressO75912.
BgeeO75912.
CleanExHS_DGKI.
GenevestigatorO75912.

Family and domain databases

Gene3D1.25.40.20. 1 hit.
InterProIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR000756. Diacylglycerol_kin_accessory.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
[Graphical view]
PfamPF00023. Ank. 2 hits.
PF00130. C1_1. 1 hit.
PF00609. DAGK_acc. 1 hit.
PF00781. DAGK_cat. 1 hit.
[Graphical view]
SMARTSM00248. ANK. 2 hits.
SM00109. C1. 2 hits.
SM00045. DAGKa. 1 hit.
SM00046. DAGKc. 1 hit.
[Graphical view]
SUPFAMSSF111331. SSF111331. 1 hit.
SSF48403. SSF48403. 1 hit.
PROSITEPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
PS50146. DAGK. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi9162.
NextBio34367.
PROO75912.
SOURCESearch...

Entry information

Entry nameDGKI_HUMAN
AccessionPrimary (citable) accession number: O75912
Secondary accession number(s): A4D1Q9, Q9NZ49
Entry history
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: November 1, 1998
Last modified: April 16, 2014
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 7

Human chromosome 7: entries, gene names and cross-references to MIM