Reviewed, UniProtKB/Swiss-Prot O75912 (DGKI_HUMAN)

Names and origin

Protein names

Recommended name:
Diacylglycerol kinase iota
Short name=DAG kinase iota
EC=2.7.1.107

Alternative name(s):
Diglyceride kinase iota
Short name=DGK-iota

Gene names

Name:

DGKI

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	1065 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.
Subcellular location	Cytoplasm. Nucleus.
Sequence similarities	Belongs to the eukaryotic diacylglycerol kinase family. Contains 2 ANK repeats. Contains 1 DAGKc domain. Contains 2 phorbol-ester/DAG-type zinc fingers.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Polymorphism
Domain	ANK repeat Repeat Zinc-finger
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	activation of protein kinase C activity by G-protein coupled receptor protein signaling pathway Inferred from electronic annotation. Source: InterPro intracellular signaling cascade Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Ref.1 Traceable author statement. Source: ProtInc nucleus Ref.1 Traceable author statement. Source: ProtInc
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW diacylglycerol kinase activity Ref.1 Traceable author statement. Source: ProtInc protein binding Inferred from physical interaction. Source: IntAct zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
VHL	P40337	1	EBI-1765520,EBI-301246
VHL	P40337-3	1	EBI-1765520,EBI-301270

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1065	1065	Diacylglycerol kinase iota		PRO_0000218466
Regions
	Domain	372 – 507	136	DAGKc
	Repeat	958 – 990	33	ANK 1
	Repeat	997 – 1026	30	ANK 2
	Zinc finger	178 – 232	55	Phorbol-ester/DAG-type 1
	Zinc finger	251 – 309	59	Phorbol-ester/DAG-type 2
	Compositional bias	20 – 31	12	Poly-Ala
	Compositional bias	69 – 74	6	Poly-Ser
	Compositional bias	95 – 102	8	Poly-Ala
Amino acid modifications
	Modified residue	1049	1	Phosphoserine Ref.4
	Modified residue	1053	1	Phosphotyrosine Ref.4
Natural variations
	Natural variant	153	1	L → F		VAR_010190
Experimental info
	Sequence conflict	160	1	A → P in AAF43006. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	O75912-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: B84971AA7630A799	FASTA	1,065	116,997
	10 20 30 40 50 60 MDAAGRGCHL LPLPAARGPA RAPAAAAAAA ASPPGPCSGA ACAPSAAAGA GAMNPSSSAG 70 80 90 100 110 120 EEKGATGGSS SSGSGAGSCC LGAEGGADPR GAGSAAAAGA AALDEPAAAG QKEKDEALEE 130 140 150 160 170 180 KLRNLTFRKQ VSYRKAISRA GLQHLAPAHP LSLPVANGPA KEPRATLDWS ENAVNGEHLW 190 200 210 220 230 240 LETNVSGDLC YLGEENCQVR FAKSALRRKC AVCKIVVHTA CIEQLEKINF RCKPTFREGG 250 260 270 280 290 300 SRSPRENFVR HHWVHRRRQE GKCKQCGKGF QQKFSFHSKE IVAISCSWCK QAFHNKVTCF 310 320 330 340 350 360 MLHHIEEPCS LGAHAAVIVP PTWIIKVKKP QNSLKASNRK KKRTSFKRKA SKRGMEQENK 370 380 390 400 410 420 GRPFVIKPIS SPLMKPLLVF VNPKSGGNQG TKVLQMFMWY LNPRQVFDLS QEGPKDALEL 430 440 450 460 470 480 YRKVPNLRIL ACGGDGTVGW ILSILDELQL SPQPPVGVLP LGTGNDLART LNWGGGYTDE 490 500 510 520 530 540 PVSKILCQVE DGTVVQLDRW NLHVERNPDL PPEELEDGVC KLPLNVFNNY FSLGFDAHVT 550 560 570 580 590 600 LEFHESREAN PEKFNSRFRN KMFYAGAAFS DFLQRSSRDL SKHVKVVCDG TDLTPKIQEL 610 620 630 640 650 660 KFQCIVFLNI PRYCAGTMPW GNPGDHHDFE PQRHDDGYIE VIGFTMASLA ALQVGGHGER 670 680 690 700 710 720 LHQCREVMLL TYKSIPMQVD GEPCRLAPAM IRISLRNQAN MVQKSKRRTS MPLLNDPQSV 730 740 750 760 770 780 PDRLRIRVNK ISLQDYEGFH YDKEKLREAS ISDWLRTIAG ELVQSFGAIP LGILVVRGDC 790 800 810 820 830 840 DLETCRMYID RLQEDLQSVS SGSQRVHYQD HETSFPRALS AQRLSPRWCF LDDRSQEHLH 850 860 870 880 890 900 FVMEISQDEI FILDPDMVVS QPAGTPPGMP DLVVEQASGI SDWWNPALRK RMLSDSGLGM 910 920 930 940 950 960 IAPYYEDSDL KDLSHSRVLQ SPVSSEDHAI LQAVIAGDLM KLIESYKNGG SLLIQGPDHC 970 980 990 1000 1010 1020 SLLHYAAKTG NGEIVKYILD HGPSELLDMA DSETGETALH KAACQRNRAV CQLLVDAGAS 1030 1040 1050 1060 LRKTDSKGKT PQERAQQAGD PDLAAYLESR QNYKVIGHED LETAV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The cloning and characterization of a novel human diacylglycerol kinase, DGK-iota." Ding L., Traer E., McIntyre T.M., Zimmerman G.A., Prescott S.M. J. Biol. Chem. 273:32746-32752(1998) [PubMed: 9830018] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Retina.
[2]	"Human chromosome 7: DNA sequence and biology." Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K., Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R., Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A., Kanematsu E., Gentles S. , Christopoulos C.C., Choufani S., Kwasnicka D., Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z., Lu F., Zeesman S., Nowaczyk M.J., Teshima I., Chitayat D., Shuman C., Weksberg R., Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J., Rahman N., Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F., Belloni E., Shaffer L.G., Pober B., Morton C.C., Gusella J.F., Bruns G.A.P., Korf B.R., Quade B.J., Ligon A.H., Ferguson H., Higgins A.W., Leach N.T., Herrick S.R., Lemyre E., Farra C.G., Kim H.-G., Summers A.M., Gripp K.W., Roberts W., Szatmari P., Winsor E.J.T., Grzeschik K.-H., Teebi A., Minassian B.A., Kere J., Armengol L., Pujana M.A., Estivill X., Wilson M.D., Koop B.F., Tosi S., Moore G.E., Boright A.P., Zlotorynski E., Kerem B., Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H., Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W., Mural R.J., Adams M.D., Tsui L.-C. Science 300:767-772(2003) [PubMed: 12690205] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Evaluation of human diacylglycerol kinase iota, DGKI, a homolog of Drosophila rdgA, in inherited retinopathy mapping to 7q." Bowne S.J., Sullivan L.S., Ding L., Traer E., Prescott S.M., Birch D.G., Kennan A., Humphries P., Daiger S.P. Mol. Vis. 6:6-9(2000) [PubMed: 10706894] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 135-1065, VARIANT PHE-153.
[4]	"Automated phosphoproteome analysis for cultured cancer cells by two-dimensional nanoLC-MS using a calcined titania/C18 biphasic column." Imami K., Sugiyama N., Kyono Y., Tomita M., Ishihama Y. Anal. Sci. 24:161-166(2008) [PubMed: 18187866] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1049 AND TYR-1053, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF061936 mRNA. Translation: AAC62010.1. CH236950 Genomic DNA. Translation: EAL24051.1. AF219939 , AF219907, AF219908, AF219909, AF219910, AF219911, AF219912, AF219913, AF219914, AF219915, AF219916, AF219917, AF219918, AF219919, AF219920, AF219921, AF219922, AF219923, AF219924, AF219925, AF219926, AF219927, AF219928, AF219929, AF219930, AF219931, AF219932, AF219933, AF219934, AF219935, AF219936, AF219937, AF219938 Genomic DNA. Translation: AAF43006.1.
IPI	IPI00027380.
RefSeq	NP_004708.1.
UniGene	Hs.242947
3D structure databases
SMR	O75912. Positions 370-692, 925-1058.
ModBase	Search...
Protein-protein interaction databases
IntAct	O75912. 2 interactions.
STRING	O75912.
PTM databases
PhosphoSite	O75912.
Proteomic databases
PRIDE	O75912.
Genome annotation databases
Ensembl	ENST00000288490; ENSP00000288490; ENSG00000157680; Homo sapiens. [Genome view]
GeneID	9162.
KEGG	hsa:9162.
UCSC	uc003vtt.1. human.
Organism-specific databases
CTD	9162.
GeneCards	GC07M136724.
H-InvDB	HIX0078758. HIX0078873.
HGNC	HGNC:2855. DGKI.
MIM	604072. gene.
PharmGKB	PA27316.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG18217.
HOVERGEN	HBG067303.
InParanoid	O75912.
PhylomeDB	O75912.
Enzyme and pathway databases
BRENDA	2.7.1.107. 247.
Gene expression databases
ArrayExpress	O75912.
Bgee	O75912.
CleanEx	HS_DGKI.
Genevestigator	O75912.
GermOnline	ENSG00000157680. Homo sapiens.
Family and domain databases
InterPro	IPR002110. Ankyrin_rpt. IPR020683. Ankyrin_rpt-contain_dom. IPR000756. Diacylglycerol_kin_accessory. IPR001206. Diacylglycerol_kinase_cat_dom. IPR002219. Prot_Kinase_C-like_PE/DAG_bd. [Graphical view]
Gene3D	G3DSA:1.25.40.20. ANK. 1 hit.
Pfam	PF00023. Ank. 1 hit. PF00130. C1_1. 1 hit. PF00609. DAGK_acc. 1 hit. PF00781. DAGK_cat. 1 hit. [Graphical view]
SMART	SM00248. ANK. 2 hits. SM00109. C1. 2 hits. SM00045. DAGKa. 1 hit. SM00046. DAGKc. 1 hit. [Graphical view]
SUPFAM	SSF48403. ANK. 1 hit.
PROSITE	PS50297. ANK_REP_REGION. 1 hit. PS50088. ANK_REPEAT. 2 hits. PS50146. DAGK. 1 hit. PS00479. ZF_DAG_PE_1. False negative. PS50081. ZF_DAG_PE_2. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	34367.
SOURCE	Search...

Entry information

Entry name

DGKI_HUMAN

Accession

Primary (citable) accession number: O75912
Secondary accession number(s): A4D1Q9, Q9NZ49

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	November 1, 1998
Last modified:	March 2, 2010
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.