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Protein

Diacylglycerol kinase iota

Gene

DGKI

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri178 – 23255Phorbol-ester/DAG-type 1Add
BLAST
Zinc fingeri251 – 30959Phorbol-ester/DAG-type 2Add
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. diacylglycerol kinase activity Source: ProtInc
  3. GTPase inhibitor activity Source: Ensembl
  4. metal ion binding Source: UniProtKB-KW
  5. NAD+ kinase activity Source: InterPro

GO - Biological processi

  1. blood coagulation Source: Reactome
  2. intracellular signal transduction Source: InterPro
  3. platelet activation Source: Reactome
  4. positive regulation of Ras protein signal transduction Source: Ensembl
  5. protein kinase C-activating G-protein coupled receptor signaling pathway Source: InterPro
  6. regulation of Rap GTPase activity Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_2202. Effects of PIP2 hydrolysis.

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol kinase iota (EC:2.7.1.107)
Short name:
DAG kinase iota
Alternative name(s):
Diglyceride kinase iota
Short name:
DGK-iota
Gene namesi
Name:DGKI
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 7

Organism-specific databases

HGNCiHGNC:2855. DGKI.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: ProtInc
  2. dendritic spine Source: MGI
  3. guanyl-nucleotide exchange factor complex Source: Ensembl
  4. nucleoplasm Source: HPA
  5. nucleus Source: ProtInc
  6. perinuclear region of cytoplasm Source: Ensembl
  7. plasma membrane Source: Reactome
  8. synapse Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA27316.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10651065Diacylglycerol kinase iotaPRO_0000218466Add
BLAST

Proteomic databases

PaxDbiO75912.
PRIDEiO75912.

PTM databases

PhosphoSiteiO75912.

Expressioni

Gene expression databases

BgeeiO75912.
CleanExiHS_DGKI.
ExpressionAtlasiO75912. baseline and differential.
GenevestigatoriO75912.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
VHLP40337-33EBI-1765520,EBI-301270

Protein-protein interaction databases

BioGridi114605. 3 interactions.
IntActiO75912. 1 interaction.
MINTiMINT-4657619.
STRINGi9606.ENSP00000288490.

Structurei

3D structure databases

ProteinModelPortaliO75912.
SMRiO75912. Positions 938-1050.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini372 – 507136DAGKcPROSITE-ProRule annotationAdd
BLAST
Repeati958 – 99033ANK 1Add
BLAST
Repeati997 – 102630ANK 2Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi20 – 3112Poly-AlaAdd
BLAST
Compositional biasi69 – 746Poly-Ser
Compositional biasi95 – 1028Poly-Ala

Sequence similaritiesi

Contains 2 ANK repeats.PROSITE-ProRule annotation
Contains 1 DAGKc domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri178 – 23255Phorbol-ester/DAG-type 1Add
BLAST
Zinc fingeri251 – 30959Phorbol-ester/DAG-type 2Add
BLAST

Keywords - Domaini

ANK repeat, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiNOG47311.
GeneTreeiENSGT00760000119050.
HOGENOMiHOG000231472.
HOVERGENiHBG067303.
InParanoidiO75912.
KOiK00901.
PhylomeDBiO75912.
TreeFamiTF312817.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR000756. Diacylglycerol_kin_accessory.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR002219. PE/DAG-bd.
[Graphical view]
PfamiPF00023. Ank. 2 hits.
PF00130. C1_1. 1 hit.
PF00609. DAGK_acc. 1 hit.
PF00781. DAGK_cat. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 2 hits.
SM00109. C1. 2 hits.
SM00045. DAGKa. 1 hit.
SM00046. DAGKc. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
SSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
PS50146. DAGK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O75912-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDAAGRGCHL LPLPAARGPA RAPAAAAAAA ASPPGPCSGA ACAPSAAAGA
60 70 80 90 100
GAMNPSSSAG EEKGATGGSS SSGSGAGSCC LGAEGGADPR GAGSAAAAGA
110 120 130 140 150
AALDEPAAAG QKEKDEALEE KLRNLTFRKQ VSYRKAISRA GLQHLAPAHP
160 170 180 190 200
LSLPVANGPA KEPRATLDWS ENAVNGEHLW LETNVSGDLC YLGEENCQVR
210 220 230 240 250
FAKSALRRKC AVCKIVVHTA CIEQLEKINF RCKPTFREGG SRSPRENFVR
260 270 280 290 300
HHWVHRRRQE GKCKQCGKGF QQKFSFHSKE IVAISCSWCK QAFHNKVTCF
310 320 330 340 350
MLHHIEEPCS LGAHAAVIVP PTWIIKVKKP QNSLKASNRK KKRTSFKRKA
360 370 380 390 400
SKRGMEQENK GRPFVIKPIS SPLMKPLLVF VNPKSGGNQG TKVLQMFMWY
410 420 430 440 450
LNPRQVFDLS QEGPKDALEL YRKVPNLRIL ACGGDGTVGW ILSILDELQL
460 470 480 490 500
SPQPPVGVLP LGTGNDLART LNWGGGYTDE PVSKILCQVE DGTVVQLDRW
510 520 530 540 550
NLHVERNPDL PPEELEDGVC KLPLNVFNNY FSLGFDAHVT LEFHESREAN
560 570 580 590 600
PEKFNSRFRN KMFYAGAAFS DFLQRSSRDL SKHVKVVCDG TDLTPKIQEL
610 620 630 640 650
KFQCIVFLNI PRYCAGTMPW GNPGDHHDFE PQRHDDGYIE VIGFTMASLA
660 670 680 690 700
ALQVGGHGER LHQCREVMLL TYKSIPMQVD GEPCRLAPAM IRISLRNQAN
710 720 730 740 750
MVQKSKRRTS MPLLNDPQSV PDRLRIRVNK ISLQDYEGFH YDKEKLREAS
760 770 780 790 800
ISDWLRTIAG ELVQSFGAIP LGILVVRGDC DLETCRMYID RLQEDLQSVS
810 820 830 840 850
SGSQRVHYQD HETSFPRALS AQRLSPRWCF LDDRSQEHLH FVMEISQDEI
860 870 880 890 900
FILDPDMVVS QPAGTPPGMP DLVVEQASGI SDWWNPALRK RMLSDSGLGM
910 920 930 940 950
IAPYYEDSDL KDLSHSRVLQ SPVSSEDHAI LQAVIAGDLM KLIESYKNGG
960 970 980 990 1000
SLLIQGPDHC SLLHYAAKTG NGEIVKYILD HGPSELLDMA DSETGETALH
1010 1020 1030 1040 1050
KAACQRNRAV CQLLVDAGAS LRKTDSKGKT PQERAQQAGD PDLAAYLESR
1060
QNYKVIGHED LETAV
Length:1,065
Mass (Da):116,997
Last modified:October 31, 1998 - v1
Checksum:iB84971AA7630A799
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti160 – 1601A → P in AAF43006 (PubMed:10706894).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti153 – 1531L → F.1 Publication
Corresponds to variant rs61757580 [ dbSNP | Ensembl ].
VAR_010190

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF061936 mRNA. Translation: AAC62010.1.
CH236950 Genomic DNA. Translation: EAL24051.1.
AH009185 Genomic DNA. Translation: AAF43006.1.
CCDSiCCDS5845.1.
RefSeqiNP_004708.1. NM_004717.2.
UniGeneiHs.242947.

Genome annotation databases

EnsembliENST00000288490; ENSP00000288490; ENSG00000157680.
GeneIDi9162.
KEGGihsa:9162.
UCSCiuc003vtt.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF061936 mRNA. Translation: AAC62010.1.
CH236950 Genomic DNA. Translation: EAL24051.1.
AH009185 Genomic DNA. Translation: AAF43006.1.
CCDSiCCDS5845.1.
RefSeqiNP_004708.1. NM_004717.2.
UniGeneiHs.242947.

3D structure databases

ProteinModelPortaliO75912.
SMRiO75912. Positions 938-1050.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114605. 3 interactions.
IntActiO75912. 1 interaction.
MINTiMINT-4657619.
STRINGi9606.ENSP00000288490.

PTM databases

PhosphoSiteiO75912.

Proteomic databases

PaxDbiO75912.
PRIDEiO75912.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000288490; ENSP00000288490; ENSG00000157680.
GeneIDi9162.
KEGGihsa:9162.
UCSCiuc003vtt.3. human.

Organism-specific databases

CTDi9162.
GeneCardsiGC07M137073.
HGNCiHGNC:2855. DGKI.
MIMi604072. gene.
neXtProtiNX_O75912.
PharmGKBiPA27316.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG47311.
GeneTreeiENSGT00760000119050.
HOGENOMiHOG000231472.
HOVERGENiHBG067303.
InParanoidiO75912.
KOiK00901.
PhylomeDBiO75912.
TreeFamiTF312817.

Enzyme and pathway databases

ReactomeiREACT_2202. Effects of PIP2 hydrolysis.

Miscellaneous databases

ChiTaRSiDGKI. human.
GenomeRNAii9162.
NextBioi34367.
PROiO75912.
SOURCEiSearch...

Gene expression databases

BgeeiO75912.
CleanExiHS_DGKI.
ExpressionAtlasiO75912. baseline and differential.
GenevestigatoriO75912.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR000756. Diacylglycerol_kin_accessory.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR002219. PE/DAG-bd.
[Graphical view]
PfamiPF00023. Ank. 2 hits.
PF00130. C1_1. 1 hit.
PF00609. DAGK_acc. 1 hit.
PF00781. DAGK_cat. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 2 hits.
SM00109. C1. 2 hits.
SM00045. DAGKa. 1 hit.
SM00046. DAGKc. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
SSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
PS50146. DAGK. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The cloning and characterization of a novel human diacylglycerol kinase, DGK-iota."
    Ding L., Traer E., McIntyre T.M., Zimmerman G.A., Prescott S.M.
    J. Biol. Chem. 273:32746-32752(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Retina.
  2. "Human chromosome 7: DNA sequence and biology."
    Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K., Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R., Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A., Kanematsu E., Gentles S.
    , Christopoulos C.C., Choufani S., Kwasnicka D., Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z., Lu F., Zeesman S., Nowaczyk M.J., Teshima I., Chitayat D., Shuman C., Weksberg R., Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J., Rahman N., Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F., Belloni E., Shaffer L.G., Pober B., Morton C.C., Gusella J.F., Bruns G.A.P., Korf B.R., Quade B.J., Ligon A.H., Ferguson H., Higgins A.W., Leach N.T., Herrick S.R., Lemyre E., Farra C.G., Kim H.-G., Summers A.M., Gripp K.W., Roberts W., Szatmari P., Winsor E.J.T., Grzeschik K.-H., Teebi A., Minassian B.A., Kere J., Armengol L., Pujana M.A., Estivill X., Wilson M.D., Koop B.F., Tosi S., Moore G.E., Boright A.P., Zlotorynski E., Kerem B., Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H., Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W., Mural R.J., Adams M.D., Tsui L.-C.
    Science 300:767-772(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "Evaluation of human diacylglycerol kinase iota, DGKI, a homolog of Drosophila rdgA, in inherited retinopathy mapping to 7q."
    Bowne S.J., Sullivan L.S., Ding L., Traer E., Prescott S.M., Birch D.G., Kennan A., Humphries P., Daiger S.P.
    Mol. Vis. 6:6-9(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 135-1065, VARIANT PHE-153.

Entry informationi

Entry nameiDGKI_HUMAN
AccessioniPrimary (citable) accession number: O75912
Secondary accession number(s): A4D1Q9, Q9NZ49
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2001
Last sequence update: October 31, 1998
Last modified: March 31, 2015
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.