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Reviewed, UniProtKB/Swiss-Prot O75909 (CCNK_HUMAN)

Last modified November 3, 2009. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Cyclin-K
Gene names
Name: CCNK
Synonyms: CPR4
OrganismHomo sapiens (Human) [Complete proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length580 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

May play a role in transcriptional regulation. In vitro, is associated with a kinase activity toward both RNA polymerase II C-terminal domain and CDK2 (CAK). Ref.9

Subunit structure

Part of a cyclin-kinase pair in the RNA polymerase II holoenzyme. Binds to CDK9.

Tissue specificity

Ubiquitously expressed. Highest levels in testis.

Sequence similarities

Belongs to the cyclin family. Cyclin C subfamily.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

ATXN1P542531EBI-739806,EBI-930964

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O75909-3)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O75909-2)

The sequence of this isoform differs from the canonical sequence as follows:
     308-354: QQPAQQQQPA...NKAAEPPPPK → LILLQGWACR...QLGGWGGQPG
     355-580: Missing.
Isoform 3 (identifier: O75909-1)

The sequence of this isoform differs from the canonical sequence as follows:
     351-357: PPPKIPK → APSQHLW
     358-580: Missing.
Isoform 4 (identifier: O75909-4)

The sequence of this isoform differs from the canonical sequence as follows:
     338-338: V → VSGLKQALGRAGFPGGGNTQV

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 580580Cyclin-K
PRO_0000080478

Regions

Compositional bias312 – 3154Poly-Gln

Amino acid modifications

Modified residue91Phosphoserine Ref.14
Modified residue3241Phosphoserine Ref.14 Ref.10
Modified residue3281Phosphoserine By similarity
Modified residue3291Phosphoserine Ref.14 Ref.10 Ref.12
Modified residue3401Phosphoserine Ref.14 Ref.11 Ref.13 Ref.15

Natural variations

Alternative sequence308 – 35447QQPAQ…PPPPK → LILLQGWACRQPATHLLPSP LEDSLLCPRPFPHPACLQLG GWGGQPG in isoform 2.
VSP_010830
Alternative sequence3381V → VSGLKQALGRAGFPGGGNTQ V in isoform 4.
VSP_035970
Alternative sequence351 – 3577PPPKIPK → APSQHLW in isoform 3.
VSP_035971
Alternative sequence355 – 580226Missing in isoform 2.
VSP_035972
Alternative sequence358 – 580223Missing in isoform 3.
VSP_035973

Experimental info

Sequence conflict1911D → T in BAD92610. Ref.7

Secondary structure

........................................... 580
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 16, 2008. Version 2.
Checksum: 8A945E90359AD9F8

FASTA58064,240
        10         20         30         40         50         60 
MKENKENSSP SVTSANLDHT KPCWYWDKKD LAHTPSQLEG LDPATEARYR REGARFIFDV 

        70         80         90        100        110        120 
GTRLGLHYDT LATGIIYFHR FYMFHSFKQF PRYVTGACCL FLAGKVEETP KKCKDIIKTA 

       130        140        150        160        170        180 
RSLLNDVQFG QFGDDPKEEV MVLERILLQT IKFDLQVEHP YQFLLKYAKQ LKGDKNKIQK 

       190        200        210        220        230        240 
LVQMAWTFVN DSLCTTLSLQ WEPEIIAVAV MYLAGRLCKF EIQEWTSKPM YRRWWEQFVQ 

       250        260        270        280        290        300 
DVPVDVLEDI CHQILDLYSQ GKQQMPHHTP HQLQQPPSLQ PTPQVPQVQQ SQPSQSSEPS 

       310        320        330        340        350        360 
QPQQKDPQQP AQQQQPAQQP KKPSPQPSSP RQVKRAVVVS PKEENKAAEP PPPKIPKIET 

       370        380        390        400        410        420 
THPPLPPAHP PPDRKPPLAA ALGEAEPPGP VDATDLPKVQ IPPPAHPAPV HQPPPLPHRP 

       430        440        450        460        470        480 
PPPPPSSYMT GMSTTSSYMS GEGYQSLQSM MKTEGPSYGA LPPAYGPPAH LPYHPHVYPP 

       490        500        510        520        530        540 
NPPPPPVPPP PASFPPPAIP PPTPGYPPPP PTYNPNFPPP PPRLPPTHAV PPHPPPGLGL 

       550        560        570        580 
PPASYPPPAV PPGGQPPVPP PIPPPGMPPV GGLGRAAWMR 

« Hide

Isoform 2.

Checksum: 39D8F18FC9429207
Show »

FASTA35440,788
Isoform 3.

Checksum: FA7DD89035A66105
Show »

FASTA35741,293
Isoform 4.

Checksum: A79E4E8B190E7199
Show »

FASTA60066,137

References

« Hide 'large scale' references
[1]"Human cyclin K, a novel RNA polymerase II-associated cyclin possessing both carboxy-terminal domain kinase and Cdk-activating kinase activity."
Edwards M.C., Wong C., Elledge S.J.
Mol. Cell. Biol. 18:4291-4300(1998) [PubMed: 9632813] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
[2]"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[3]NIEHS SNPs program
Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"The DNA sequence and analysis of human chromosome 14."
Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H. expand/collapse author list , Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J.
Nature 421:601-607(2003) [PubMed: 12508121] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Uterus.
[6]Elledge S., Gerber S., Rozet J., Perrault I., Ducroq D., Munnich A., Kaplan J.
Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-348 (ISOFORM 1/3).
[7]Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S., Ohara O., Nagase T., Kikuno R.F.
Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 191-580 (ISOFORM 4).
Tissue: Brain.
[8]"Full-length cDNA libraries and normalization."
Li W.B., Gruber C., Jessee J., Polayes D.
Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 279-580 (ISOFORM 4).
Tissue: Neuroblastoma.
[9]"Cyclin K functions as a CDK9 regulatory subunit and participates in RNA polymerase II transcription."
Fu T.J., Peng J., Lee G., Price D.H., Flores O.
J. Biol. Chem. 274:34527-34530(1999) [PubMed: 10574912] [Abstract]
Cited for: FUNCTION.
Tissue: T-cell.
[10]"Large-scale characterization of HeLa cell nuclear phosphoproteins."
Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J., Li J., Cohn M.A., Cantley L.C., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004) [PubMed: 15302935] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-324 AND SER-329, MASS SPECTROMETRY.
Tissue: Epithelium.
[11]"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
Cell 127:635-648(2006) [PubMed: 17081983] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-340, MASS SPECTROMETRY.
Tissue: Epithelium.
[12]"Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra."
Yu L.-R., Zhu Z., Chan K.C., Issaq H.J., Dimitrov D.S., Veenstra T.D.
J. Proteome Res. 6:4150-4162(2007) [PubMed: 17924679] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-329, MASS SPECTROMETRY.
Tissue: Epithelium.
[13]"Automated phosphoproteome analysis for cultured cancer cells by two-dimensional nanoLC-MS using a calcined titania/C18 biphasic column."
Imami K., Sugiyama N., Kyono Y., Tomita M., Ishihama Y.
Anal. Sci. 24:161-166(2008) [PubMed: 18187866] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-340, MASS SPECTROMETRY.
[14]"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-9; SER-324; SER-329 AND SER-340, MASS SPECTROMETRY.
[15]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-340, MASS SPECTROMETRY.
[16]Colinge J., Superti-Furga G., Bennett K.L.
Submitted (OCT-2008) to UniProtKB
Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[17]"Crystal structure of human cyclin K, a positive regulator of cyclin-dependent kinase 9."
Baek K., Brown R.S., Birrane G., Ladias J.A.
J. Mol. Biol. 366:563-573(2007) [PubMed: 17169370] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) OF 11-267.
+Additional computationally mapped references.

Web resources

Cross-references

Sequence databases

AF060515 mRNA. Translation: AAD09978.1.
BT006950 mRNA. Translation: AAP35596.1.
AF542236 Genomic DNA. Translation: AAN06829.1.
AL110504 Genomic DNA. No translation available.
BC015935 mRNA. Translation: AAH15935.1.
AF270832 expand/collapse EMBL AC list , AF270825, AF270826, AF270827, AF270828, AF270829, AF270830, AF270831 Genomic DNA. Translation: AAF82290.1.
BX247958 mRNA. Translation: CAD62298.1.
AB209373 Transcribed RNA. Translation: BAD92610.1.
IPIIPI00431127.
IPI00783214.
IPI00915260.
IPI00939345.
RefSeqNP_001092872.1.
NP_003849.2.
UniGeneHs.510409

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
2I53X-ray1.50A11-267[»]
ModBaseSearch...

Protein-protein interaction databases

IntActO75909. 16 interactions.
STRINGO75909.

PTM databases

PhosphoSiteO75909.

Proteomic databases

PRIDEO75909.

Genome annotation databases

EnsemblENST00000216279; ENSP00000216279; ENSG00000090061; Homo sapiens. [Genome view]
ENST00000380246; ENSP00000369596; ENSG00000090061; Homo sapiens. [Genome view]
ENST00000389879; ENSP00000374529; ENSG00000090061; Homo sapiens. [Genome view]
ENST00000411691; ENSP00000398898; ENSG00000090061; Homo sapiens. [Genome view]
ENST00000437596; ENSP00000395705; ENSG00000090061; Homo sapiens. [Genome view]
GeneID8812.
KEGGhsa:8812.
UCSCuc001ygg.2. human.
uc001ygi.2. human.

Organism-specific databases

CTD8812.
GeneCardsGC14P099017.
H-InvDBHIX0011959.
HGNCHGNC:1596. CCNK.
HPAHPA000645.
MIM603544. gene.
PharmGKBPA26161.
GenAtlasSearch...

Phylogenomic databases

HOGENOMO75909.
HOVERGENO75909.
OMAYITGMST.

Gene expression databases

ArrayExpressO75909.
BgeeO75909.
CleanExHS_CCNK.
GenevestigatorO75909.
GermOnlineENSG00000090061. Homo sapiens.

Family and domain databases

InterProIPR006670. Cyclin.
IPR006671. Cyclin_N.
IPR013763. Cyclin_related.
[Graphical view]
Gene3DG3DSA:1.10.472.10. Cyclin_related. 1 hit.
PfamPF00134. Cyclin_N. 1 hit.
[Graphical view]
SMARTSM00385. CYCLIN. 2 hits.
[Graphical view]
PROSITEPS00292. CYCLINS. False negative.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio33052.
SOURCESearch...

Entry information

Entry nameCCNK_HUMAN
AccessionPrimary (citable) accession number: O75909
Secondary accession number(s): Q59FT6 expand/collapse secondary AC list , Q86U16, Q96B63, Q9NNY9
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 16, 2008
Last modified: November 3, 2009
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

Human chromosome 14

Human chromosome 14: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents