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O75908 (SOAT2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 115. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sterol O-acyltransferase 2

EC=2.3.1.26
Alternative name(s):
Acyl-coenzyme A:cholesterol acyltransferase 2
Short name=ACAT-2
Cholesterol acyltransferase 2
Gene names
Name:SOAT2
Synonyms:ACACT2, ACAT2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length522 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Plays a role in lipoprotein assembly and dietary cholesterol absorption. In addition to its acyltransferase activity, it may act as a ligase. May provide cholesteryl esters for lipoprotein secretion from hepatocytes and intestinal mucosa.

Catalytic activity

Acyl-CoA + cholesterol = CoA + cholesterol ester.

Subunit structure

May form homo- or heterodimers By similarity.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily.

Ontologies

Keywords
   Biological processCholesterol metabolism
Lipid metabolism
Steroid metabolism
Sterol metabolism
   Cellular componentEndoplasmic reticulum
Membrane
   Coding sequence diversityPolymorphism
   DomainTransmembrane
Transmembrane helix
   Molecular functionAcyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcholesterol efflux

Inferred from mutant phenotype PubMed 15308631. Source: BHF-UCL

cholesterol esterification

Inferred from direct assay PubMed 16647063. Source: BHF-UCL

cholesterol homeostasis

Traceable author statement Ref.4. Source: BHF-UCL

cholesterol metabolic process

Inferred from direct assay PubMed 16647063. Source: BHF-UCL

intestinal cholesterol absorption

Inferred by curator PubMed 14615411. Source: BHF-UCL

macrophage derived foam cell differentiation

Traceable author statement PubMed 14615411. Source: BHF-UCL

very-low-density lipoprotein particle assembly

Inferred from mutant phenotype PubMed 15308631. Source: BHF-UCL

   Cellular_componentbrush border

Inferred from direct assay PubMed 14615411. Source: BHF-UCL

endoplasmic reticulum

Inferred from direct assay PubMed 15451793. Source: BHF-UCL

endoplasmic reticulum membrane

Inferred from direct assay PubMed 12808042. Source: BHF-UCL

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functioncholesterol O-acyltransferase activity

Inferred from direct assay PubMed 16647063. Source: BHF-UCL

cholesterol binding

Inferred from direct assay PubMed 16647063. Source: BHF-UCL

fatty-acyl-CoA binding

Inferred from direct assay PubMed 16647063. Source: BHF-UCL

transferase activity, transferring acyl groups

Traceable author statement Ref.1. Source: ProtInc

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 522522Sterol O-acyltransferase 2
PRO_0000207645

Regions

Transmembrane124 – 14421Helical; Potential
Transmembrane155 – 17521Helical; Potential
Transmembrane200 – 22021Helical; Potential
Transmembrane262 – 28221Helical; Potential
Transmembrane304 – 32421Helical; Potential
Transmembrane344 – 36623Helical; Potential
Transmembrane437 – 45721Helical; Potential
Transmembrane472 – 49221Helical; Potential

Sites

Active site4341 Potential

Natural variations

Natural variant141E → G.
Corresponds to variant rs9658625 [ dbSNP | Ensembl ].
VAR_020373
Natural variant2541T → I. Ref.2 Ref.5
Corresponds to variant rs2272296 [ dbSNP | Ensembl ].
VAR_020374

Experimental info

Sequence conflict221R → P in AAK48829. Ref.4

Sequences

Sequence LengthMass (Da)Tools
O75908 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: EEAC2DB569FFE729

FASTA52259,896
        10         20         30         40         50         60 
MEPGGARLRL QRTEGLGGER ERQPCGDGNT ETHRAPDLVQ WTRHMEAVKA QLLEQAQGQL 

        70         80         90        100        110        120 
RELLDRAMRE AIQSYPSQDK PLPPPPPGSL SRTQEPSLGK QKVFIIRKSL LDELMEVQHF 

       130        140        150        160        170        180 
RTIYHMFIAG LCVFIISTLA IDFIDEGRLL LEFDLLIFSF GQLPLALVTW VPMFLSTLLA 

       190        200        210        220        230        240 
PYQALRLWAR GTWTQATGLG CALLAAHAVV LCALPVHVAV EHQLPPASRC VLVFEQVRFL 

       250        260        270        280        290        300 
MKSYSFLREA VPGTLRARRG EGIQAPSFSS YLYFLFCPTL IYRETYPRTP YVRWNYVAKN 

       310        320        330        340        350        360 
FAQALGCVLY ACFILGRLCV PVFANMSREP FSTRALVLSI LHATLPGIFM LLLIFFAFLH 

       370        380        390        400        410        420 
CWLNAFAEML RFGDRMFYRD WWNSTSFSNY YRTWNVVVHD WLYSYVYQDG LRLLGARARG 

       430        440        450        460        470        480 
VAMLGVFLVS AVAHEYIFCF VLGFFYPVML ILFLVIGGML NFMMHDQRTG PAWNVLMWTM 

       490        500        510        520 
LFLGQGIQVS LYCQEWYARR HCPLPQATFW GLVTPRSWSC HT 

« Hide

References

« Hide 'large scale' references
[1]"Characterization of two human genes encoding acyl coenzyme A:cholesterol acyltransferase-related enzymes."
Oelkers P., Behari A., Cromley D., Billheimer J.T., Sturley S.L.
J. Biol. Chem. 273:26765-26771(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Immunological quantitation and localization of ACAT-1 and ACAT-2 in human liver and small intestine."
Chang C.C.Y., Sakashita N., Ornvold K., Lee O., Chang E.T., Dong R., Lin S., Lee C.-Y.G., Strom S.C., Kashyap R., Fung J.J., Farese R.V. Jr., Patoiseau J.-F., Delhon A., Chang T.-Y.
J. Biol. Chem. 275:28083-28092(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ILE-254.
Tissue: Intestine.
[3]"Structure of the human acyl-CoA:cholesterol acyltransferase-2 (ACAT-2) gene and its relation to dyslipidemia."
Katsuren K., Tamura T., Arashiro R., Takata K., Matsuura T., Niikawa N., Ohta T.
Biochim. Biophys. Acta 1531:230-240(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"Organization of human ACAT-2 gene and its cell-type-specific promoter activity."
Song B.L., Qi W., Yang X.Y., Chang C.C.Y., Zhu J.Q., Chang T.Y., Li B.L.
Biochem. Biophys. Res. Commun. 282:580-588(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ILE-254.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF059203 mRNA. Translation: AAC63998.1.
AF099031 mRNA. Translation: AAC78335.2.
AF331516 expand/collapse EMBL AC list , AF331502, AF331503, AF331504, AF331505, AF331506, AF331507, AF331508, AF331509, AF331510, AF331511, AF331512, AF331513, AF331514, AF331515 Genomic DNA. Translation: AAK18275.1.
AF332858, AF332857 Genomic DNA. Translation: AAK48829.1.
BC096091 mRNA. Translation: AAH96091.1.
BC096092 mRNA. Translation: AAH96092.1.
CCDSCCDS8847.1.
RefSeqNP_003569.1. NM_003578.3.
UniGeneHs.656544.

3D structure databases

ProteinModelPortalO75908.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid114015. 2 interactions.
STRING9606.ENSP00000301466.

Chemistry

BindingDBO75908.
ChEMBLCHEMBL4465.

PTM databases

PhosphoSiteO75908.

Proteomic databases

PaxDbO75908.
PRIDEO75908.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000301466; ENSP00000301466; ENSG00000167780.
GeneID8435.
KEGGhsa:8435.
UCSCuc001sbv.3. human.

Organism-specific databases

CTD8435.
GeneCardsGC12P053497.
HGNCHGNC:11178. SOAT2.
HPAHPA049462.
MIM601311. gene.
neXtProtNX_O75908.
PharmGKBPA36016.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5056.
HOGENOMHOG000020782.
HOVERGENHBG058198.
InParanoidO75908.
KOK00637.
OMALMEVQHF.
PhylomeDBO75908.
TreeFamTF315226.

Enzyme and pathway databases

BRENDA2.3.1.26. 2681.

Gene expression databases

ArrayExpressO75908.
BgeeO75908.
CleanExHS_ACAT2.
HS_SOAT2.
GenevestigatorO75908.

Family and domain databases

InterProIPR004299. MBOAT_fam.
IPR014371. Oat_ACAT_DAG_ARE.
[Graphical view]
PANTHERPTHR10408. PTHR10408. 1 hit.
PfamPF03062. MBOAT. 1 hit.
[Graphical view]
PIRSFPIRSF000439. Oat_ACAT_DAG_ARE. 1 hit.
ProtoNetSearch...

Other

GeneWikiSOAT2.
GenomeRNAi8435.
NextBio31558.
PROO75908.
SOURCESearch...

Entry information

Entry nameSOAT2_HUMAN
AccessionPrimary (citable) accession number: O75908
Secondary accession number(s): Q4VB99, Q96TD4, Q9UNR2
Entry history
Integrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: November 1, 1998
Last modified: July 9, 2014
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 12

Human chromosome 12: entries, gene names and cross-references to MIM