Reviewed, UniProtKB/Swiss-Prot O75907 (DGAT1_HUMAN)

Names and origin

Protein names

Recommended name:
    Diacylglycerol O-acyltransferase 1
    EC=2.3.1.20
Alternative name(s):
    Diglyceride acyltransferase
    ACAT-related gene product 1

Gene names

Name:	DGAT1
Synonyms:	AGRP1, DGAT

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	488 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. In contrast to DGAT2 it is not essential for survival. May be involved in VLDL (very low density lipoprotein) assembly.
Catalytic activity	Acyl-CoA + 1,2-diacylglycerol = CoA + triacylglycerol.
Pathway	Lipid metabolism; glycerolipid metabolism.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Molecular function	Acyltransferase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	triglyceride biosynthetic process Inferred from direct assay. Source: UniProtKB very-low-density lipoprotein particle assembly Inferred from mutant phenotype. Source: UniProtKB
Cellular component	cytosol Inferred from Experiment. Source: Reactome endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	diacylglycerol O-acyltransferase activity Inferred from Experiment. Source: Reactome
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 488	488	Diacylglycerol O-acyltransferase 1		PRO_0000207654
Regions
	Transmembrane	104 – 124	21	Potential
	Transmembrane	130 – 150	21	Potential
	Transmembrane	166 – 186	21	Potential
	Transmembrane	189 – 209	21	Potential
	Transmembrane	282 – 302	21	Potential
	Transmembrane	332 – 352	21	Potential
	Transmembrane	406 – 426	21	Potential
	Transmembrane	428 – 448	21	Potential
	Transmembrane	453 – 473	21	Potential
Sites
	Active site	415	1	Potential
Amino acid modifications
	Modified residue	17	1	Phosphoserine By similarity
Experimental info
	Sequence conflict	129	1	Y → H in AAC63997. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O75907-1 [UniParc]. Last modified July 25, 2003. Version 2. Checksum: 6574D5DBF15D6171	FASTA	488	55,278
	10 20 30 40 50 60 MGDRGSSRRR RTGSRPSSHG GGGPAAAEEE VRDAAAGPDV GAAGDAPAPA PNKDGDAGVG 70 80 90 100 110 120 SGHWELRCHR LQDSLFSSDS GFSNYRGILN WCVVMLILSN ARLFLENLIK YGILVDPIQV 130 140 150 160 170 180 VSLFLKDPYS WPAPCLVIAA NVFAVAAFQV EKRLAVGALT EQAGLLLHVA NLATILCFPA 190 200 210 220 230 240 AVVLLVESIT PVGSLLALMA HTILFLKLFS YRDVNSWCRR ARAKAASAGK KASSAAAPHT 250 260 270 280 290 300 VSYPDNLTYR DLYYFLFAPT LCYELNFPRS PRIRKRFLLR RILEMLFFTQ LQVGLIQQWM 310 320 330 340 350 360 VPTIQNSMKP FKDMDYSRII ERLLKLAVPN HLIWLIFFYW LFHSCLNAVA ELMQFGDREF 370 380 390 400 410 420 YRDWWNSESV TYFWQNWNIP VHKWCIRHFY KPMLRRGSSK WMARTGVFLA SAFFHEYLVS 430 440 450 460 470 480 VPLRMFRLWA FTGMMAQIPL AWFVGRFFQG NYGNAAVWLS LIIGQPIAVL MYVHDYYVLN YEAPAAEA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of two human genes encoding acyl coenzyme A:cholesterol acyltransferase-related enzymes." Oelkers P., Behari A., Cromley D., Billheimer J.T., Sturley S.L. J. Biol. Chem. 273:26765-26771(1998) [PubMed: 9756920] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	Yamasaki Y., Watanabe T.K., Tanigami A. Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain and Skin.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF059202 mRNA. Translation: AAC63997.1. AB057815 mRNA. Translation: BAC66170.1. BC015762 mRNA. Translation: AAH15762.1. BC023565 mRNA. Translation: AAH23565.1.
IPI	IPI00015799.
RefSeq	NP_036211.2. XP_001719426.1.
UniGene	Hs.613075
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	O75907.
Proteomic databases
PRIDE	O75907.
Genome annotation databases
Ensembl	ENSG00000185000. Homo sapiens. [Contig view] ENSG00000215711. Homo sapiens. [Contig view]
GeneID	8694.
KEGG	hsa:727765. hsa:8694.
Organism-specific databases
GeneCards	GC08M145510. GC08P9K0011.
H-InvDB	HIX0007854. HIX0025693.
HGNC	HGNC:2843. DGAT1.
MIM	604900. gene.
PharmGKB	PA27303.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	O75907.
HOVERGEN	O75907.
OMA	O75907. GRFFQGN.
Enzyme and pathway databases
BRENDA	2.3.1.20. 247.
Reactome	REACT_602. Lipid and lipoprotein metabolism.
Gene expression databases
Bgee	O75907.
CleanEx	HS_DGAT1.
GermOnline	ENSG00000185000. Homo sapiens.
Family and domain databases
InterPro	IPR004299. MBOAT_fam. [Graphical view]
Pfam	PF03062. MBOAT. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	275012.
SOURCE	Search...

Entry information

Entry name

DGAT1_HUMAN

Accession

Primary (citable) accession number: O75907
Secondary accession number(s): Q96BB8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 18, 2001
Last sequence update:	July 25, 2003
Last modified:	June 16, 2009
This is version 77 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)