Skip Header

Contribute Send feedback
Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot O75907 (DGAT1_HUMAN)

Last modified January 19, 2010. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Diacylglycerol O-acyltransferase 1
    EC=2.3.1.20
Alternative name(s):
    Diglyceride acyltransferase
    ACAT-related gene product 1
Gene names
Name: DGAT1
Synonyms: AGRP1, DGAT
OrganismHomo sapiens (Human) [Complete proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length488 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level.

General annotation (Comments)

Function

Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. In contrast to DGAT2 it is not essential for survival. May be involved in VLDL (very low density lipoprotein) assembly.

Catalytic activity

Acyl-CoA + 1,2-diacylglycerol = CoA + triacylglycerol.

Pathway

Lipid metabolism; glycerolipid metabolism.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.

Sequence similarities

Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 488488Diacylglycerol O-acyltransferase 1
PRO_0000207654

Regions

Transmembrane104 – 12421 Potential
Transmembrane130 – 15021 Potential
Transmembrane166 – 18621 Potential
Transmembrane189 – 20921 Potential
Transmembrane282 – 30221 Potential
Transmembrane332 – 35221 Potential
Transmembrane406 – 42621 Potential
Transmembrane428 – 44821 Potential
Transmembrane453 – 47321 Potential

Sites

Active site4151 Potential

Amino acid modifications

Modified residue171Phosphoserine By similarity

Experimental info

Sequence conflict1291Y → H in AAC63997. Ref.1

Sequences

Sequence LengthMass (Da)Tools
O75907-1 [UniParc].

Last modified July 25, 2003. Version 2.
Checksum: 6574D5DBF15D6171

FASTA48855,278
        10         20         30         40         50         60 
MGDRGSSRRR RTGSRPSSHG GGGPAAAEEE VRDAAAGPDV GAAGDAPAPA PNKDGDAGVG 

        70         80         90        100        110        120 
SGHWELRCHR LQDSLFSSDS GFSNYRGILN WCVVMLILSN ARLFLENLIK YGILVDPIQV 

       130        140        150        160        170        180 
VSLFLKDPYS WPAPCLVIAA NVFAVAAFQV EKRLAVGALT EQAGLLLHVA NLATILCFPA 

       190        200        210        220        230        240 
AVVLLVESIT PVGSLLALMA HTILFLKLFS YRDVNSWCRR ARAKAASAGK KASSAAAPHT 

       250        260        270        280        290        300 
VSYPDNLTYR DLYYFLFAPT LCYELNFPRS PRIRKRFLLR RILEMLFFTQ LQVGLIQQWM 

       310        320        330        340        350        360 
VPTIQNSMKP FKDMDYSRII ERLLKLAVPN HLIWLIFFYW LFHSCLNAVA ELMQFGDREF 

       370        380        390        400        410        420 
YRDWWNSESV TYFWQNWNIP VHKWCIRHFY KPMLRRGSSK WMARTGVFLA SAFFHEYLVS 

       430        440        450        460        470        480 
VPLRMFRLWA FTGMMAQIPL AWFVGRFFQG NYGNAAVWLS LIIGQPIAVL MYVHDYYVLN 


YEAPAAEA 

« Hide

References

« Hide 'large scale' references
[1]"Characterization of two human genes encoding acyl coenzyme A:cholesterol acyltransferase-related enzymes."
Oelkers P., Behari A., Cromley D., Billheimer J.T., Sturley S.L.
J. Biol. Chem. 273:26765-26771(1998) [PubMed: 9756920] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]Yamasaki Y., Watanabe T.K., Tanigami A.
Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain and Skin.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF059202 mRNA. Translation: AAC63997.1.
AB057815 mRNA. Translation: BAC66170.1.
CH471162 Genomic DNA. Translation: EAW82127.1.
BC015762 mRNA. Translation: AAH15762.1.
BC023565 mRNA. Translation: AAH23565.1.
BC150649 mRNA. Translation: AAI50650.1.
IPIIPI00015799.
RefSeqNP_036211.2.
UniGeneHs.571397

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGO75907.

PTM databases

PhosphoSiteO75907.

Proteomic databases

PRIDEO75907.

Genome annotation databases

EnsemblENST00000332324; ENSP00000332258; ENSG00000185000; Homo sapiens. [Genome view]
GeneID8694.
KEGGhsa:8694.
UCSCuc003zbv.2. human.

Organism-specific databases

CTD8694.
GeneCardsGC08M145510.
GC08P9K0011.
H-InvDBHIX0007854.
HIX0025693.
HGNCHGNC:2843. DGAT1.
MIM604900. gene.
PharmGKBPA27303.
GenAtlasSearch...

Phylogenomic databases

eggNOGprNOG14033.
HOGENOMHBG326701.
HOVERGENO75907.
InParanoidO75907.
OMAGRFFQGN.
OrthoDBEOG9NCPZM.
PhylomeDBO75907.

Enzyme and pathway databases

BRENDA2.3.1.20. 247.
ReactomeREACT_602. Metabolism of lipids and lipoproteins.

Gene expression databases

ArrayExpressO75907.
BgeeO75907.
CleanExHS_DGAT1.
GenevestigatorO75907.
GermOnlineENSG00000185000. Homo sapiens.

Family and domain databases

InterProIPR004299. MBOAT_fam.
[Graphical view]
PfamPF03062. MBOAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio275012.
SOURCESearch...

Entry information

Entry nameDGAT1_HUMAN
AccessionPrimary (citable) accession number: O75907
Secondary accession number(s): B2RWQ2, Q96BB8
Entry history
Integrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: July 25, 2003
Last modified: January 19, 2010
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

Human chromosome 8

Human chromosome 8: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents