SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

O75907

- DGAT1_HUMAN

UniProt

O75907 - DGAT1_HUMAN

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Diacylglycerol O-acyltransferase 1
Gene
DGAT1, AGRP1, DGAT
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. In contrast to DGAT2 it is not essential for survival. May be involved in VLDL (very low density lipoprotein) assembly. In liver, plays a role in esterifying exogenous fatty acids to glycerol. Functions as the major acyl-CoA retinol acyltransferase (ARAT) in the skin, where it acts to maintain retinoid homeostasis and prevent retinoid toxicity leading to skin and hair disorders.

Catalytic activityi

Acyl-CoA + 1,2-diacylglycerol = CoA + triacylglycerol.1 Publication
Acyl-CoA + retinol = CoA + retinyl ester.1 Publication

Enzyme regulationi

XP620 is a selective DGAT1 inhibitor.1 Publication

Kineticsi

  1. KM=25.9 µM for retinol1 Publication
  2. KM=13.9 µM for palmitoyl coenzyme A

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei415 – 4151 By similarity

GO - Molecular functioni

  1. 2-acylglycerol O-acyltransferase activity Source: Ensembl
  2. diacylglycerol O-acyltransferase activity Source: MGI
  3. protein binding Source: IntAct
  4. retinol O-fatty-acyltransferase activity Source: UniProtKB-EC
  5. transferase activity, transferring acyl groups Source: ProtInc

GO - Biological processi

  1. acylglycerol acyl-chain remodeling Source: Reactome
  2. cellular lipid metabolic process Source: Reactome
  3. diacylglycerol metabolic process Source: BHF-UCL
  4. fatty acid homeostasis Source: Ensembl
  5. glycerophospholipid biosynthetic process Source: Reactome
  6. lipid storage Source: BHF-UCL
  7. long-chain fatty-acyl-CoA metabolic process Source: BHF-UCL
  8. phospholipid metabolic process Source: Reactome
  9. small molecule metabolic process Source: Reactome
  10. triglyceride biosynthetic process Source: UniProtKB
  11. triglyceride metabolic process Source: ProtInc
  12. very-low-density lipoprotein particle assembly Source: BHF-UCL
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Enzyme and pathway databases

ReactomeiREACT_1190. Triglyceride Biosynthesis.
REACT_121122. Acyl chain remodeling of DAG and TAG.
UniPathwayiUPA00230.

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol O-acyltransferase 1 (EC:2.3.1.20)
Alternative name(s):
ACAT-related gene product 1
Acyl-CoA retinol O-fatty-acyltransferase (EC:2.3.1.76)
Short name:
ARAT
Short name:
Retinol O-fatty-acyltransferase
Diglyceride acyltransferase
Gene namesi
Name:DGAT1
Synonyms:AGRP1, DGAT
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 8

Organism-specific databases

HGNCiHGNC:2843. DGAT1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 8787Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei88 – 10922Helical; Reviewed prediction
Add
BLAST
Topological domaini110 – 220111Lumenal Reviewed prediction
Add
BLAST
Transmembranei221 – 2299Helical; Reviewed prediction
Topological domaini230 – 2301Cytoplasmic Reviewed prediction
Transmembranei231 – 2399Helical; Reviewed prediction
Topological domaini240 – 488249Lumenal Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: Reactome
  2. integral component of membrane Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Organism-specific databases

Orphaneti329242. Congenital chronic diarrhea with protein-losing enteropathy.
PharmGKBiPA27303.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 488488Diacylglycerol O-acyltransferase 1
PRO_0000207654Add
BLAST

Proteomic databases

MaxQBiO75907.
PaxDbiO75907.
PRIDEiO75907.

PTM databases

PhosphoSiteiO75907.

Expressioni

Gene expression databases

ArrayExpressiO75907.
BgeeiO75907.
CleanExiHS_DGAT1.
GenevestigatoriO75907.

Organism-specific databases

HPAiCAB032853.

Interactioni

Subunit structurei

Homodimer or homotetramer By similarity.

Binary interactionsi

WithEntry#Exp.IntActNotes
Q99IB82EBI-3906527,EBI-6927873From a different organism.

Protein-protein interaction databases

BioGridi114241. 5 interactions.
IntActiO75907. 3 interactions.
STRINGi9606.ENSP00000332258.

Structurei

3D structure databases

ProteinModelPortaliO75907.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 9191Involved in homomerization By similarity
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5056.
HOGENOMiHOG000213917.
HOVERGENiHBG051341.
InParanoidiO75907.
KOiK11155.
OMAiLWAFTAM.
PhylomeDBiO75907.
TreeFamiTF314921.

Family and domain databases

InterProiIPR027251. Diacylglycerol_acylTrfase1.
IPR004299. MBOAT_fam.
IPR014371. Oat_ACAT_DAG_ARE.
[Graphical view]
PANTHERiPTHR10408. PTHR10408. 1 hit.
PfamiPF03062. MBOAT. 1 hit.
[Graphical view]
PIRSFiPIRSF000439. Oat_ACAT_DAG_ARE. 1 hit.
PIRSF500231. Oat_dag. 1 hit.

Sequencei

Sequence statusi: Complete.

O75907-1 [UniParc]FASTAAdd to Basket

« Hide

MGDRGSSRRR RTGSRPSSHG GGGPAAAEEE VRDAAAGPDV GAAGDAPAPA    50
PNKDGDAGVG SGHWELRCHR LQDSLFSSDS GFSNYRGILN WCVVMLILSN 100
ARLFLENLIK YGILVDPIQV VSLFLKDPYS WPAPCLVIAA NVFAVAAFQV 150
EKRLAVGALT EQAGLLLHVA NLATILCFPA AVVLLVESIT PVGSLLALMA 200
HTILFLKLFS YRDVNSWCRR ARAKAASAGK KASSAAAPHT VSYPDNLTYR 250
DLYYFLFAPT LCYELNFPRS PRIRKRFLLR RILEMLFFTQ LQVGLIQQWM 300
VPTIQNSMKP FKDMDYSRII ERLLKLAVPN HLIWLIFFYW LFHSCLNAVA 350
ELMQFGDREF YRDWWNSESV TYFWQNWNIP VHKWCIRHFY KPMLRRGSSK 400
WMARTGVFLA SAFFHEYLVS VPLRMFRLWA FTGMMAQIPL AWFVGRFFQG 450
NYGNAAVWLS LIIGQPIAVL MYVHDYYVLN YEAPAAEA 488
Length:488
Mass (Da):55,278
Last modified:July 25, 2003 - v2
Checksum:i6574D5DBF15D6171
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti129 – 1291Y → H in AAC63997. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF059202 mRNA. Translation: AAC63997.1.
AB057815 mRNA. Translation: BAC66170.1.
CH471162 Genomic DNA. Translation: EAW82127.1.
CH471162 Genomic DNA. Translation: EAW82129.1.
BC015762 mRNA. Translation: AAH15762.1.
BC023565 mRNA. Translation: AAH23565.1.
BC150649 mRNA. Translation: AAI50650.1.
CCDSiCCDS6420.1.
RefSeqiNP_036211.2. NM_012079.5.
UniGeneiHs.521954.

Genome annotation databases

EnsembliENST00000332324; ENSP00000332258; ENSG00000185000.
ENST00000565657; ENSP00000454624; ENSG00000261698.
GeneIDi8694.
KEGGihsa:8694.
UCSCiuc003zbv.3. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF059202 mRNA. Translation: AAC63997.1 .
AB057815 mRNA. Translation: BAC66170.1 .
CH471162 Genomic DNA. Translation: EAW82127.1 .
CH471162 Genomic DNA. Translation: EAW82129.1 .
BC015762 mRNA. Translation: AAH15762.1 .
BC023565 mRNA. Translation: AAH23565.1 .
BC150649 mRNA. Translation: AAI50650.1 .
CCDSi CCDS6420.1.
RefSeqi NP_036211.2. NM_012079.5.
UniGenei Hs.521954.

3D structure databases

ProteinModelPortali O75907.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 114241. 5 interactions.
IntActi O75907. 3 interactions.
STRINGi 9606.ENSP00000332258.

Chemistry

BindingDBi O75907.
ChEMBLi CHEMBL6009.

PTM databases

PhosphoSitei O75907.

Proteomic databases

MaxQBi O75907.
PaxDbi O75907.
PRIDEi O75907.

Protocols and materials databases

DNASUi 8694.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000332324 ; ENSP00000332258 ; ENSG00000185000 .
ENST00000565657 ; ENSP00000454624 ; ENSG00000261698 .
GeneIDi 8694.
KEGGi hsa:8694.
UCSCi uc003zbv.3. human.

Organism-specific databases

CTDi 8694.
GeneCardsi GC08M145510.
HGNCi HGNC:2843. DGAT1.
HPAi CAB032853.
MIMi 604900. gene.
neXtProti NX_O75907.
Orphaneti 329242. Congenital chronic diarrhea with protein-losing enteropathy.
PharmGKBi PA27303.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG5056.
HOGENOMi HOG000213917.
HOVERGENi HBG051341.
InParanoidi O75907.
KOi K11155.
OMAi LWAFTAM.
PhylomeDBi O75907.
TreeFami TF314921.

Enzyme and pathway databases

UniPathwayi UPA00230 .
Reactomei REACT_1190. Triglyceride Biosynthesis.
REACT_121122. Acyl chain remodeling of DAG and TAG.

Miscellaneous databases

ChiTaRSi DGAT1. human.
GenomeRNAii 8694.
NextBioi 32607.
PROi O75907.
SOURCEi Search...

Gene expression databases

ArrayExpressi O75907.
Bgeei O75907.
CleanExi HS_DGAT1.
Genevestigatori O75907.

Family and domain databases

InterProi IPR027251. Diacylglycerol_acylTrfase1.
IPR004299. MBOAT_fam.
IPR014371. Oat_ACAT_DAG_ARE.
[Graphical view ]
PANTHERi PTHR10408. PTHR10408. 1 hit.
Pfami PF03062. MBOAT. 1 hit.
[Graphical view ]
PIRSFi PIRSF000439. Oat_ACAT_DAG_ARE. 1 hit.
PIRSF500231. Oat_dag. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of two human genes encoding acyl coenzyme A:cholesterol acyltransferase-related enzymes."
    Oelkers P., Behari A., Cromley D., Billheimer J.T., Sturley S.L.
    J. Biol. Chem. 273:26765-26771(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Yamasaki Y., Watanabe T.K., Tanigami A.
    Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain and Skin.
  5. "Acyl coenzyme A dependent retinol esterification by acyl coenzyme A: diacylglycerol acyltransferase 1."
    Orland M.D., Anwar K., Cromley D., Chu C.H., Chen L., Billheimer J.T., Hussain M.M., Cheng D.
    Biochim. Biophys. Acta 1737:76-82(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION.

Entry informationi

Entry nameiDGAT1_HUMAN
AccessioniPrimary (citable) accession number: O75907
Secondary accession number(s): B2RWQ2, D3DWL6, Q96BB8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: July 25, 2003
Last modified: September 3, 2014
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi