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O75907

- DGAT1_HUMAN

UniProt

O75907 - DGAT1_HUMAN

Protein

Diacylglycerol O-acyltransferase 1

Gene

DGAT1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 2 (25 Jul 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. In contrast to DGAT2 it is not essential for survival. May be involved in VLDL (very low density lipoprotein) assembly. In liver, plays a role in esterifying exogenous fatty acids to glycerol. Functions as the major acyl-CoA retinol acyltransferase (ARAT) in the skin, where it acts to maintain retinoid homeostasis and prevent retinoid toxicity leading to skin and hair disorders.

    Catalytic activityi

    Acyl-CoA + 1,2-diacylglycerol = CoA + triacylglycerol.1 Publication
    Acyl-CoA + retinol = CoA + retinyl ester.1 Publication

    Enzyme regulationi

    XP620 is a selective DGAT1 inhibitor.1 Publication

    Kineticsi

    1. KM=25.9 µM for retinol1 Publication
    2. KM=13.9 µM for palmitoyl coenzyme A1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei415 – 4151By similarity

    GO - Molecular functioni

    1. 2-acylglycerol O-acyltransferase activity Source: Ensembl
    2. diacylglycerol O-acyltransferase activity Source: MGI
    3. protein binding Source: IntAct
    4. retinol O-fatty-acyltransferase activity Source: UniProtKB-EC
    5. transferase activity, transferring acyl groups Source: ProtInc

    GO - Biological processi

    1. acylglycerol acyl-chain remodeling Source: Reactome
    2. cellular lipid metabolic process Source: Reactome
    3. diacylglycerol metabolic process Source: BHF-UCL
    4. fatty acid homeostasis Source: Ensembl
    5. glycerophospholipid biosynthetic process Source: Reactome
    6. lipid storage Source: BHF-UCL
    7. long-chain fatty-acyl-CoA metabolic process Source: BHF-UCL
    8. phospholipid metabolic process Source: Reactome
    9. small molecule metabolic process Source: Reactome
    10. triglyceride biosynthetic process Source: UniProtKB
    11. triglyceride metabolic process Source: ProtInc
    12. very-low-density lipoprotein particle assembly Source: BHF-UCL

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Enzyme and pathway databases

    ReactomeiREACT_1190. Triglyceride Biosynthesis.
    REACT_121122. Acyl chain remodeling of DAG and TAG.
    UniPathwayiUPA00230.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Diacylglycerol O-acyltransferase 1 (EC:2.3.1.20)
    Alternative name(s):
    ACAT-related gene product 1
    Acyl-CoA retinol O-fatty-acyltransferase (EC:2.3.1.76)
    Short name:
    ARAT
    Short name:
    Retinol O-fatty-acyltransferase
    Diglyceride acyltransferase
    Gene namesi
    Name:DGAT1
    Synonyms:AGRP1, DGAT
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 8

    Organism-specific databases

    HGNCiHGNC:2843. DGAT1.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: Reactome
    2. integral component of membrane Source: BHF-UCL

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    Pathology & Biotechi

    Organism-specific databases

    Orphaneti329242. Congenital chronic diarrhea with protein-losing enteropathy.
    PharmGKBiPA27303.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 488488Diacylglycerol O-acyltransferase 1PRO_0000207654Add
    BLAST

    Proteomic databases

    MaxQBiO75907.
    PaxDbiO75907.
    PRIDEiO75907.

    PTM databases

    PhosphoSiteiO75907.

    Expressioni

    Gene expression databases

    ArrayExpressiO75907.
    BgeeiO75907.
    CleanExiHS_DGAT1.
    GenevestigatoriO75907.

    Organism-specific databases

    HPAiCAB032853.

    Interactioni

    Subunit structurei

    Homodimer or homotetramer.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Q99IB82EBI-3906527,EBI-6927873From a different organism.

    Protein-protein interaction databases

    BioGridi114241. 5 interactions.
    IntActiO75907. 3 interactions.
    STRINGi9606.ENSP00000332258.

    Structurei

    3D structure databases

    ProteinModelPortaliO75907.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 8787CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini110 – 220111LumenalSequence AnalysisAdd
    BLAST
    Topological domaini230 – 2301CytoplasmicSequence Analysis
    Topological domaini240 – 488249LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei88 – 10922HelicalSequence AnalysisAdd
    BLAST
    Transmembranei221 – 2299HelicalSequence Analysis
    Transmembranei231 – 2399HelicalSequence Analysis

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 9191Involved in homomerizationBy similarityAdd
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG5056.
    HOGENOMiHOG000213917.
    HOVERGENiHBG051341.
    InParanoidiO75907.
    KOiK11155.
    OMAiLWAFTAM.
    PhylomeDBiO75907.
    TreeFamiTF314921.

    Family and domain databases

    InterProiIPR027251. Diacylglycerol_acylTrfase1.
    IPR004299. MBOAT_fam.
    IPR014371. Oat_ACAT_DAG_ARE.
    [Graphical view]
    PANTHERiPTHR10408. PTHR10408. 1 hit.
    PfamiPF03062. MBOAT. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000439. Oat_ACAT_DAG_ARE. 1 hit.
    PIRSF500231. Oat_dag. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O75907-1 [UniParc]FASTAAdd to Basket

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    MGDRGSSRRR RTGSRPSSHG GGGPAAAEEE VRDAAAGPDV GAAGDAPAPA    50
    PNKDGDAGVG SGHWELRCHR LQDSLFSSDS GFSNYRGILN WCVVMLILSN 100
    ARLFLENLIK YGILVDPIQV VSLFLKDPYS WPAPCLVIAA NVFAVAAFQV 150
    EKRLAVGALT EQAGLLLHVA NLATILCFPA AVVLLVESIT PVGSLLALMA 200
    HTILFLKLFS YRDVNSWCRR ARAKAASAGK KASSAAAPHT VSYPDNLTYR 250
    DLYYFLFAPT LCYELNFPRS PRIRKRFLLR RILEMLFFTQ LQVGLIQQWM 300
    VPTIQNSMKP FKDMDYSRII ERLLKLAVPN HLIWLIFFYW LFHSCLNAVA 350
    ELMQFGDREF YRDWWNSESV TYFWQNWNIP VHKWCIRHFY KPMLRRGSSK 400
    WMARTGVFLA SAFFHEYLVS VPLRMFRLWA FTGMMAQIPL AWFVGRFFQG 450
    NYGNAAVWLS LIIGQPIAVL MYVHDYYVLN YEAPAAEA 488
    Length:488
    Mass (Da):55,278
    Last modified:July 25, 2003 - v2
    Checksum:i6574D5DBF15D6171
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti129 – 1291Y → H in AAC63997. (PubMed:9756920)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF059202 mRNA. Translation: AAC63997.1.
    AB057815 mRNA. Translation: BAC66170.1.
    CH471162 Genomic DNA. Translation: EAW82127.1.
    CH471162 Genomic DNA. Translation: EAW82129.1.
    BC015762 mRNA. Translation: AAH15762.1.
    BC023565 mRNA. Translation: AAH23565.1.
    BC150649 mRNA. Translation: AAI50650.1.
    CCDSiCCDS6420.1.
    RefSeqiNP_036211.2. NM_012079.5.
    UniGeneiHs.521954.

    Genome annotation databases

    EnsembliENST00000332324; ENSP00000332258; ENSG00000185000.
    GeneIDi8694.
    KEGGihsa:8694.
    UCSCiuc003zbv.3. human.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF059202 mRNA. Translation: AAC63997.1 .
    AB057815 mRNA. Translation: BAC66170.1 .
    CH471162 Genomic DNA. Translation: EAW82127.1 .
    CH471162 Genomic DNA. Translation: EAW82129.1 .
    BC015762 mRNA. Translation: AAH15762.1 .
    BC023565 mRNA. Translation: AAH23565.1 .
    BC150649 mRNA. Translation: AAI50650.1 .
    CCDSi CCDS6420.1.
    RefSeqi NP_036211.2. NM_012079.5.
    UniGenei Hs.521954.

    3D structure databases

    ProteinModelPortali O75907.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 114241. 5 interactions.
    IntActi O75907. 3 interactions.
    STRINGi 9606.ENSP00000332258.

    Chemistry

    BindingDBi O75907.
    ChEMBLi CHEMBL6009.

    PTM databases

    PhosphoSitei O75907.

    Proteomic databases

    MaxQBi O75907.
    PaxDbi O75907.
    PRIDEi O75907.

    Protocols and materials databases

    DNASUi 8694.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000332324 ; ENSP00000332258 ; ENSG00000185000 .
    GeneIDi 8694.
    KEGGi hsa:8694.
    UCSCi uc003zbv.3. human.

    Organism-specific databases

    CTDi 8694.
    GeneCardsi GC08M145510.
    HGNCi HGNC:2843. DGAT1.
    HPAi CAB032853.
    MIMi 604900. gene.
    neXtProti NX_O75907.
    Orphaneti 329242. Congenital chronic diarrhea with protein-losing enteropathy.
    PharmGKBi PA27303.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG5056.
    HOGENOMi HOG000213917.
    HOVERGENi HBG051341.
    InParanoidi O75907.
    KOi K11155.
    OMAi LWAFTAM.
    PhylomeDBi O75907.
    TreeFami TF314921.

    Enzyme and pathway databases

    UniPathwayi UPA00230 .
    Reactomei REACT_1190. Triglyceride Biosynthesis.
    REACT_121122. Acyl chain remodeling of DAG and TAG.

    Miscellaneous databases

    ChiTaRSi DGAT1. human.
    GenomeRNAii 8694.
    NextBioi 32607.
    PROi O75907.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O75907.
    Bgeei O75907.
    CleanExi HS_DGAT1.
    Genevestigatori O75907.

    Family and domain databases

    InterProi IPR027251. Diacylglycerol_acylTrfase1.
    IPR004299. MBOAT_fam.
    IPR014371. Oat_ACAT_DAG_ARE.
    [Graphical view ]
    PANTHERi PTHR10408. PTHR10408. 1 hit.
    Pfami PF03062. MBOAT. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000439. Oat_ACAT_DAG_ARE. 1 hit.
    PIRSF500231. Oat_dag. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of two human genes encoding acyl coenzyme A:cholesterol acyltransferase-related enzymes."
      Oelkers P., Behari A., Cromley D., Billheimer J.T., Sturley S.L.
      J. Biol. Chem. 273:26765-26771(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. Yamasaki Y., Watanabe T.K., Tanigami A.
      Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain and Skin.
    5. "Acyl coenzyme A dependent retinol esterification by acyl coenzyme A: diacylglycerol acyltransferase 1."
      Orland M.D., Anwar K., Cromley D., Chu C.H., Chen L., Billheimer J.T., Hussain M.M., Cheng D.
      Biochim. Biophys. Acta 1737:76-82(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION.

    Entry informationi

    Entry nameiDGAT1_HUMAN
    AccessioniPrimary (citable) accession number: O75907
    Secondary accession number(s): B2RWQ2, D3DWL6, Q96BB8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 18, 2001
    Last sequence update: July 25, 2003
    Last modified: October 1, 2014
    This is version 128 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 8
      Human chromosome 8: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3