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O75888 (TNF13_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 136. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tumor necrosis factor ligand superfamily member 13
Alternative name(s):
A proliferation-inducing ligand
Short name=APRIL
TNF- and APOL-related leukocyte expressed ligand 2
Short name=TALL-2
TNF-related death ligand 1
Short name=TRDL-1
CD_antigen=CD256
Gene names
Name:TNFSF13
Synonyms:APRIL, TALL2, ZTNF2
ORF Names:UNQ383/PRO715
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length250 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cytokine that binds to TNFRSF13B/TACI and to TNFRSF17/BCMA. Plays a role in the regulation of tumor cell growth. May be involved in monocyte/macrophage-mediated immunological processes. Ref.12

Subunit structure

Homotrimer Potential.

Subcellular location

Secreted Ref.13.

Tissue specificity

Expressed at high levels in transformed cell lines, cancers of colon, thyroid, lymphoid tissues and specifically expressed in monocytes and macrophages.

Induction

Down-regulated by phorbol myristate acetate/ionomycin treatment.

Post-translational modification

The precursor is cleaved by furin. Ref.13

Sequence similarities

Belongs to the tumor necrosis factor family.

Alternative products

This entry describes 6 isoforms produced by alternative splicing. [Align] [Select]
Isoform Alpha (identifier: O75888-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Beta (identifier: O75888-2)

The sequence of this isoform differs from the canonical sequence as follows:
     113-129: KQHSVLHLVPINATSKD → N
Isoform Gamma (identifier: O75888-3)

The sequence of this isoform differs from the canonical sequence as follows:
     247-249: Missing.
Isoform 4 (identifier: O75888-4)

The sequence of this isoform differs from the canonical sequence as follows:
     86-113: Missing.
Note: No experimental confirmation available.
Isoform TWE-PRIL (identifier: O43508-2)

The sequence of this isoform can be found in the external entry O43508.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Isoform 5 (identifier: O75888-5)

The sequence of this isoform differs from the canonical sequence as follows:
     1-17: Missing.
     87-114: Missing.
Note: Gene prediction based on EST data.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Propeptide1 – 104104
PRO_0000034524
Chain105 – 250146Tumor necrosis factor ligand superfamily member 13
PRO_0000034525

Sites

Site104 – 1052Cleavage; by furin

Amino acid modifications

Glycosylation1241N-linked (GlcNAc...) Potential
Disulfide bond196 ↔ 211 Potential

Natural variations

Alternative sequence1 – 1717Missing in isoform 5.
VSP_046725
Alternative sequence86 – 11328Missing in isoform 4.
VSP_043154
Alternative sequence87 – 11428Missing in isoform 5.
VSP_046726
Alternative sequence113 – 12917KQHSV…ATSKD → N in isoform Beta.
VSP_006450
Alternative sequence247 – 2493Missing in isoform Gamma.
VSP_006451
Natural variant671G → R. Ref.9
Corresponds to variant rs11552708 [ dbSNP | Ensembl ].
VAR_052586
Natural variant961N → S. Ref.11
Corresponds to variant rs3803800 [ dbSNP | Ensembl ].
VAR_052587

Experimental info

Mutagenesis101 – 1044RKRR → AKRA: Abolishes proteolytic processing. Ref.13

Sequences

Sequence LengthMass (Da)Tools
Isoform Alpha [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: AE1A6B9457F6E298

FASTA25027,433
        10         20         30         40         50         60 
MPASSPFLLA PKGPPGNMGG PVREPALSVA LWLSWGAALG AVACAMALLT QQTELQSLRR 

        70         80         90        100        110        120 
EVSRLQGTGG PSQNGEGYPW QSLPEQSSDA LEAWENGERS RKRRAVLTQK QKKQHSVLHL 

       130        140        150        160        170        180 
VPINATSKDD SDVTEVMWQP ALRRGRGLQA QGYGVRIQDA GVYLLYSQVL FQDVTFTMGQ 

       190        200        210        220        230        240 
VVSREGQGRQ ETLFRCIRSM PSHPDRAYNS CYSAGVFHLH QGDILSVIIP RARAKLNLSP 

       250 
HGTFLGFVKL 

« Hide

Isoform Beta [UniParc].

Checksum: 9F0012E23EBC0780
Show »

FASTA23425,678
Isoform Gamma [UniParc].

Checksum: BAB7F6E2989D9DC8
Show »

FASTA24727,059
Isoform 4 [UniParc].

Checksum: BF3EA9A2F5AB4106
Show »

FASTA22224,151
Isoform TWE-PRIL [UniParc].

See O43508.

Isoform 5 [UniParc].

Checksum: 98217A3D1F58D3FA
Show »

FASTA20522,488

References

« Hide 'large scale' references
[1]"APRIL, a new ligand of the tumor necrosis factor family, stimulates tumor cell growth."
Hahne M., Kataoka T., Schroeter M., Hofmann K., Irmler M., Bodmer J.-L., Schneider P., Bornand T., Holler N., French L.E., Sordat B., Rimoldi D., Tschopp J.
J. Exp. Med. 188:1185-1190(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Uterus.
[2]"TALL-1 is a novel member of the TNF family that is down-regulated by mitogens."
Shu H.-B., Hu W.-H., Johnson H.
J. Leukoc. Biol. 65:680-683(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Homo sapiens tumor necrosis factor homolog."
Farrah T., Grant F., Haldeman B., Whitmore T., Gross J., O'Hara P.
Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]"APRIL/TRDL-1, a tumor necrosis factor-like ligand, stimulates cell death."
Kelly K.A., Manos E.J., Jensen G.T., Nadauld L., Jones D.A.
Cancer Res. 60:1021-1027(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS ALPHA; BETA AND GAMMA).
[5]"An endogenous hybrid mRNA encodes TWE-PRIL, a functional cell surface TWEAK-APRIL fusion protein."
Pradet-Balade B., Medema J.P., Lopez-Fraga M., Lozano J.C., Kolfschoten G.M., Picard A., Martinez-A C., Garcia-Sanz J.A., Hahne M.
EMBO J. 21:5711-5720(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM ALPHA).
[6]"Polymorphisms of human APRIL gene."
Kawasaki A., Tsuchiya N., Kusaoi M., Murakami T., Fukazawa T., Matsuta K., Hashimoto H., Tokunaga K.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[7]"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment."
Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. expand/collapse author list , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.
Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM ALPHA).
[8]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
Tissue: Spleen.
[9]"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM ALPHA), VARIANT ARG-67.
[10]"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L. expand/collapse author list , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[11]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM GAMMA), VARIANT SER-96.
Tissue: Ovary.
[12]"APRIL and TALL-I and receptors BCMA and TACI: system for regulating humoral immunity."
Yu G., Boone T., Delaney J., Hawkins N., Kelley M.J., Ramakrishnan M., McCabe S., Qiu W.R., Kornuc M., Xia X.-Z., Guo J., Stolina M., Boyle W.J., Sarosi I., Hsu H., Senaldi G., Theill L.E.
Nat. Immunol. 1:252-256(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[13]"Biologically active APRIL is secreted following intracellular processing in the Golgi apparatus by furin convertase."
Lopez-Fraga M., Fernandez R., Albar J.P., Hahne M.
EMBO Rep. 2:945-951(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEOLYTIC PROCESSING BY FURIN, MUTAGENESIS OF 101-ARG--ARG-104, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF046888 mRNA. Translation: AAC61312.1.
AF136294 mRNA. Translation: AAD29422.1.
AF184972 mRNA. Translation: AAF01321.1.
AF114011 mRNA. Translation: AAF59828.1.
AF114012 mRNA. Translation: AAF59829.1.
AF114013 mRNA. Translation: AAF59830.1.
AY358880 mRNA. Translation: AAQ89239.1.
AY081050 mRNA. Translation: AAL90442.1.
AB222992 Genomic DNA. Translation: BAE16556.1.
AK301221 mRNA. Translation: BAG62794.1.
BT019561 mRNA. Translation: AAV38368.1.
BT019562 mRNA. Translation: AAV38369.1.
AC016876 Genomic DNA. No translation available.
BC008042 mRNA. Translation: AAH08042.1.
RefSeqNP_001185552.1. NM_001198623.1.
NP_001185553.1. NM_001198624.1.
NP_003799.1. NM_003808.3.
NP_742084.1. NM_172087.2.
NP_742085.1. NM_172088.2.
UniGeneHs.54673.

3D structure databases

ProteinModelPortalO75888.
SMRO75888. Positions 114-250.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid114278. 10 interactions.
IntActO75888. 2 interactions.
STRING9606.ENSP00000343505.

PTM databases

PhosphoSiteO75888.

Proteomic databases

PaxDbO75888.
PRIDEO75888.

Protocols and materials databases

DNASU8741.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000338784; ENSP00000343505; ENSG00000161955. [O75888-1]
ENST00000349228; ENSP00000314455; ENSG00000161955. [O75888-2]
ENST00000380535; ENSP00000369908; ENSG00000161955. [O75888-4]
ENST00000396542; ENSP00000379792; ENSG00000161955. [O75888-5]
ENST00000396545; ENSP00000379794; ENSG00000161955. [O75888-3]
GeneID8741.
KEGGhsa:8741.
UCSCuc002ghj.2. human. [O75888-3]
uc002ghk.2. human. [O75888-1]
uc002ghl.2. human. [O75888-2]
uc010vua.1. human. [O75888-4]

Organism-specific databases

CTD8741.
GeneCardsGC17P007461.
HGNCHGNC:11928. TNFSF13.
HPAHPA004863.
MIM604472. gene.
neXtProtNX_O75888.
PharmGKBPA36621.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG45253.
HOGENOMHOG000036810.
HOVERGENHBG104367.
KOK05475.
OMAKGPPGNM.
OrthoDBEOG7T4MM3.
PhylomeDBO75888.
TreeFamTF332331.

Enzyme and pathway databases

ReactomeREACT_21257. Metabolism of RNA.
REACT_71. Gene Expression.

Gene expression databases

BgeeO75888.
CleanExHS_TNFSF13.
GenevestigatorO75888.

Family and domain databases

Gene3D2.60.120.40. 1 hit.
InterProIPR021184. TNF_CS.
IPR006052. TNF_dom.
IPR008983. Tumour_necrosis_fac-like_dom.
[Graphical view]
PfamPF00229. TNF. 1 hit.
[Graphical view]
SMARTSM00207. TNF. 1 hit.
[Graphical view]
SUPFAMSSF49842. SSF49842. 1 hit.
PROSITEPS00251. TNF_1. 1 hit.
PS50049. TNF_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiTNFSF13.
GenomeRNAi8741.
NextBio32793.
PROO75888.
SOURCESearch...

Entry information

Entry nameTNF13_HUMAN
AccessionPrimary (citable) accession number: O75888
Secondary accession number(s): A8MYD5 expand/collapse secondary AC list , B4DVT2, Q541E1, Q5U0G8, Q96HV6, Q9P1M8, Q9P1M9
Entry history
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: November 1, 1998
Last modified: April 16, 2014
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 17

Human chromosome 17: entries, gene names and cross-references to MIM

Human cell differentiation molecules

CD nomenclature of surface proteins of human leucocytes and list of entries