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Protein

Signal transducing adapter molecule 2

Gene

STAM2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in intracellular signal transduction mediated by cytokines and growth factors. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and MYC induction. May also play a role in T-cell development. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with HGS (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes (By similarity).By similarity

GO - Biological processi

Keywordsi

Biological processProtein transport, Transport

Enzyme and pathway databases

ReactomeiR-HSA-182971. EGFR downregulation.
R-HSA-5689880. Ub-specific processing proteases.
R-HSA-6807004. Negative regulation of MET activity.
R-HSA-8856825. Cargo recognition for clathrin-mediated endocytosis.
R-HSA-8856828. Clathrin-mediated endocytosis.
R-HSA-8875360. InlB-mediated entry of Listeria monocytogenes into host cell.
R-HSA-917729. Endosomal Sorting Complex Required For Transport (ESCRT).
SIGNORiO75886.

Names & Taxonomyi

Protein namesi
Recommended name:
Signal transducing adapter molecule 2
Short name:
STAM-2
Alternative name(s):
Hrs-binding protein
Gene namesi
Name:STAM2
Synonyms:HBP
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:11358. STAM2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: LIFEdb
  • cytosol Source: HPA
  • early endosome membrane Source: UniProtKB-SubCell
  • ESCRT-0 complex Source: ParkinsonsUK-UCL
  • intracellular membrane-bounded organelle Source: HPA
  • nucleoplasm Source: HPA

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi10254.
OpenTargetsiENSG00000115145.
PharmGKBiPA36180.

Polymorphism and mutation databases

BioMutaiSTAM2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001901471 – 525Signal transducing adapter molecule 2Add BLAST525

Post-translational modificationi

Phosphorylated in response to IL-2, GM-CSF, EGF and PDGF.

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO75886.
MaxQBiO75886.
PaxDbiO75886.
PeptideAtlasiO75886.
PRIDEiO75886.

PTM databases

iPTMnetiO75886.
PhosphoSitePlusiO75886.

Expressioni

Tissue specificityi

Ubiquitously expressed.1 Publication

Gene expression databases

BgeeiENSG00000115145.
CleanExiHS_STAM2.
GenevisibleiO75886. HS.

Organism-specific databases

HPAiHPA035528.
HPA035529.

Interactioni

Subunit structurei

Component of the ESCRT-0 complex composed of STAM or STAM2 and HGS. Part of a complex at least composed of HSG, STAM2 and EPS15. Interacts with JAK2 and JAK3. Interacts with ubiquitinated proteins and the deubiquitinating enzyme USP8/UBPY (By similarity). Interacts (via the via the PxVxL motif) with CBX5; the interaction is direct. Interacts with VPS37C. Interacts with ubiquitin; the interaction is direct. Interacts (via UIM domain) with UBQLN1 (via ubiquitin-like domain) (By similarity).By similarity4 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi115548. 61 interactors.
IntActiO75886. 38 interactors.
MINTiMINT-145850.
STRINGi9606.ENSP00000263904.

Structurei

Secondary structure

1525
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi10 – 16Combined sources7
Helixi26 – 38Combined sources13
Beta strandi39 – 41Combined sources3
Helixi42 – 54Combined sources13
Helixi59 – 75Combined sources17
Helixi78 – 84Combined sources7
Helixi87 – 98Combined sources12
Helixi103 – 119Combined sources17
Turni120 – 122Combined sources3
Turni124 – 126Combined sources3
Helixi127 – 137Combined sources11
Turni138 – 140Combined sources3
Beta strandi199 – 201Combined sources3
Beta strandi205 – 211Combined sources7
Beta strandi217 – 219Combined sources3
Beta strandi228 – 233Combined sources6
Beta strandi237 – 244Combined sources8
Beta strandi247 – 251Combined sources5
Helixi253 – 255Combined sources3
Beta strandi256 – 258Combined sources3
Helixi352 – 357Combined sources6
Helixi359 – 365Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1X2QNMR-A195-269[»]
1X5BNMR-A1-150[»]
2L0TNMR-B1-150[»]
5CRVX-ray2.00C/D350-370[»]
5IXFNMR-A162-265[»]
ProteinModelPortaliO75886.
SMRiO75886.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO75886.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini16 – 144VHSPROSITE-ProRule annotationAdd BLAST129
Domaini165 – 184UIMPROSITE-ProRule annotationAdd BLAST20
Domaini202 – 261SH3PROSITE-ProRule annotationAdd BLAST60
Domaini360 – 377ITAMAdd BLAST18

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni219 – 220Interaction with USP8By similarity2
Regioni334 – 368Interaction with HGSBy similarityAdd BLAST35

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi54 – 67PxVxL motifAdd BLAST14

Domaini

The VHS and UIM domains mediate the interaction with ubiquitinated proteins.
The SH3 domain mediates the interaction with USP8.
Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain.

Sequence similaritiesi

Belongs to the STAM family.Curated

Keywords - Domaini

SH3 domain

Phylogenomic databases

eggNOGiKOG2199. Eukaryota.
ENOG410XTSY. LUCA.
GeneTreeiENSGT00870000136415.
HOGENOMiHOG000231952.
HOVERGENiHBG053175.
InParanoidiO75886.
KOiK04705.
OMAiYTQQMGM.
OrthoDBiEOG091G0H1Y.
PhylomeDBiO75886.
TreeFamiTF315007.

Family and domain databases

Gene3Di1.25.40.90. 1 hit.
InterProiView protein in InterPro
IPR008942. ENTH_VHS.
IPR001452. SH3_domain.
IPR003903. UIM_dom.
IPR002014. VHS_dom.
PfamiView protein in Pfam
PF00018. SH3_1. 1 hit.
PF02809. UIM. 1 hit.
PF00790. VHS. 1 hit.
PRINTSiPR00452. SH3DOMAIN.
SMARTiView protein in SMART
SM00326. SH3. 1 hit.
SM00726. UIM. 1 hit.
SM00288. VHS. 1 hit.
SUPFAMiSSF48464. SSF48464. 1 hit.
SSF50044. SSF50044. 1 hit.
PROSITEiView protein in PROSITE
PS50002. SH3. 1 hit.
PS50330. UIM. 1 hit.
PS50179. VHS. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O75886-1) [UniParc]FASTAAdd to basket
Also known as: STAM2A

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPLFTANPFE QDVEKATNEY NTTEDWSLIM DICDKVGSTP NGAKDCLKAI
60 70 80 90 100
MKRVNHKVPH VALQALTLLG ACVANCGKIF HLEVCSRDFA TEVRAVIKNK
110 120 130 140 150
AHPKVCEKLK SLMVEWSEEF QKDPQFSLIS ATIKSMKEEG ITFPPAGSQT
160 170 180 190 200
VSAAAKNGTS SNKNKEDEDI AKAIELSLQE QKQQHTETKS LYPSSEIQLN
210 220 230 240 250
NKVARKVRAL YDFEAVEDNE LTFKHGEIII VLDDSDANWW KGENHRGIGL
260 270 280 290 300
FPSNFVTTNL NIETEAAAVD KLNVIDDDVE EIKKSEPEPV YIDEDKMDRA
310 320 330 340 350
LQVLQSIDPT DSKPDSQDLL DLEDICQQMG PMIDEKLEEI DRKHSELSEL
360 370 380 390 400
NVKVLEALEL YNKLVNEAPV YSVYSKLHPP AHYPPASSGV PMQTYPVQSH
410 420 430 440 450
GGNYMGQSIH QVTVAQSYSL GPDQIGPLRS LPPNVNSSVT AQPAQTSYLS
460 470 480 490 500
TGQDTVSNPT YMNQNSNLQS ATGTTAYTQQ MGMSVDMSSY QNTTSNLPQL
510 520
AGFPVTVPAH PVAQQHTNYH QQPLL
Length:525
Mass (Da):58,164
Last modified:November 1, 1998 - v1
Checksum:i408D484544DD9403
GO
Isoform 2 (identifier: O75886-2) [UniParc]FASTAAdd to basket
Also known as: STAM2B

The sequence of this isoform differs from the canonical sequence as follows:
     343-525: Missing.

Show »
Length:342
Mass (Da):38,370
Checksum:iE54540C48B6CD1B3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti81 – 83HLE → RLG in CAB63735 (PubMed:11230166).Curated3
Sequence conflicti254N → D in CAB63735 (PubMed:11230166).Curated1
Sequence conflicti266A → V in CAB63735 (PubMed:11230166).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_014848343 – 525Missing in isoform 2. 1 PublicationAdd BLAST183

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF042273 mRNA. Translation: AAC63963.1.
AF042274 mRNA. Translation: AAC63964.1.
AL133600 mRNA. Translation: CAB63735.1.
AK292265 mRNA. Translation: BAF84954.1.
AK292847 mRNA. Translation: BAF85536.1.
AC079790 Genomic DNA. Translation: AAY14712.1.
CH471058 Genomic DNA. Translation: EAX11490.1.
CH471058 Genomic DNA. Translation: EAX11492.1.
BC028740 mRNA. Translation: AAH28740.1.
CCDSiCCDS2196.1. [O75886-1]
PIRiT43437.
RefSeqiNP_005834.4. NM_005843.5. [O75886-1]
UniGeneiHs.17200.

Genome annotation databases

EnsembliENST00000263904; ENSP00000263904; ENSG00000115145. [O75886-1]
GeneIDi10254.
KEGGihsa:10254.
UCSCiuc002tyc.4. human. [O75886-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF042273 mRNA. Translation: AAC63963.1.
AF042274 mRNA. Translation: AAC63964.1.
AL133600 mRNA. Translation: CAB63735.1.
AK292265 mRNA. Translation: BAF84954.1.
AK292847 mRNA. Translation: BAF85536.1.
AC079790 Genomic DNA. Translation: AAY14712.1.
CH471058 Genomic DNA. Translation: EAX11490.1.
CH471058 Genomic DNA. Translation: EAX11492.1.
BC028740 mRNA. Translation: AAH28740.1.
CCDSiCCDS2196.1. [O75886-1]
PIRiT43437.
RefSeqiNP_005834.4. NM_005843.5. [O75886-1]
UniGeneiHs.17200.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1X2QNMR-A195-269[»]
1X5BNMR-A1-150[»]
2L0TNMR-B1-150[»]
5CRVX-ray2.00C/D350-370[»]
5IXFNMR-A162-265[»]
ProteinModelPortaliO75886.
SMRiO75886.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115548. 61 interactors.
IntActiO75886. 38 interactors.
MINTiMINT-145850.
STRINGi9606.ENSP00000263904.

PTM databases

iPTMnetiO75886.
PhosphoSitePlusiO75886.

Polymorphism and mutation databases

BioMutaiSTAM2.

Proteomic databases

EPDiO75886.
MaxQBiO75886.
PaxDbiO75886.
PeptideAtlasiO75886.
PRIDEiO75886.

Protocols and materials databases

DNASUi10254.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000263904; ENSP00000263904; ENSG00000115145. [O75886-1]
GeneIDi10254.
KEGGihsa:10254.
UCSCiuc002tyc.4. human. [O75886-1]

Organism-specific databases

CTDi10254.
DisGeNETi10254.
GeneCardsiSTAM2.
HGNCiHGNC:11358. STAM2.
HPAiHPA035528.
HPA035529.
MIMi606244. gene.
neXtProtiNX_O75886.
OpenTargetsiENSG00000115145.
PharmGKBiPA36180.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2199. Eukaryota.
ENOG410XTSY. LUCA.
GeneTreeiENSGT00870000136415.
HOGENOMiHOG000231952.
HOVERGENiHBG053175.
InParanoidiO75886.
KOiK04705.
OMAiYTQQMGM.
OrthoDBiEOG091G0H1Y.
PhylomeDBiO75886.
TreeFamiTF315007.

Enzyme and pathway databases

ReactomeiR-HSA-182971. EGFR downregulation.
R-HSA-5689880. Ub-specific processing proteases.
R-HSA-6807004. Negative regulation of MET activity.
R-HSA-8856825. Cargo recognition for clathrin-mediated endocytosis.
R-HSA-8856828. Clathrin-mediated endocytosis.
R-HSA-8875360. InlB-mediated entry of Listeria monocytogenes into host cell.
R-HSA-917729. Endosomal Sorting Complex Required For Transport (ESCRT).
SIGNORiO75886.

Miscellaneous databases

ChiTaRSiSTAM2. human.
EvolutionaryTraceiO75886.
GeneWikiiSTAM2.
GenomeRNAii10254.
PROiPR:O75886.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000115145.
CleanExiHS_STAM2.
GenevisibleiO75886. HS.

Family and domain databases

Gene3Di1.25.40.90. 1 hit.
InterProiView protein in InterPro
IPR008942. ENTH_VHS.
IPR001452. SH3_domain.
IPR003903. UIM_dom.
IPR002014. VHS_dom.
PfamiView protein in Pfam
PF00018. SH3_1. 1 hit.
PF02809. UIM. 1 hit.
PF00790. VHS. 1 hit.
PRINTSiPR00452. SH3DOMAIN.
SMARTiView protein in SMART
SM00326. SH3. 1 hit.
SM00726. UIM. 1 hit.
SM00288. VHS. 1 hit.
SUPFAMiSSF48464. SSF48464. 1 hit.
SSF50044. SSF50044. 1 hit.
PROSITEiView protein in PROSITE
PS50002. SH3. 1 hit.
PS50330. UIM. 1 hit.
PS50179. VHS. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSTAM2_HUMAN
AccessioniPrimary (citable) accession number: O75886
Secondary accession number(s): A8K8A0
, D3DPA1, Q7LDQ0, Q9UF58
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: November 1, 1998
Last modified: March 15, 2017
This is version 165 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.