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O75843

- AP1G2_HUMAN

UniProt

O75843 - AP1G2_HUMAN

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Protein

AP-1 complex subunit gamma-like 2

Gene

AP1G2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

May function in protein sorting in late endosomes or multivesucular bodies (MVBs). Involved in MVB-assisted maturation of hepatitis B virus (HBV).3 Publications

GO - Molecular functioni

  1. protein transporter activity Source: InterPro

GO - Biological processi

  1. intracellular protein transport Source: InterPro
  2. vesicle-mediated transport Source: UniProtKB
  3. viral process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
AP-1 complex subunit gamma-like 2
Alternative name(s):
Gamma2-adaptin
Short name:
G2ad
Gene namesi
Name:AP1G2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 14

Organism-specific databases

HGNCiHGNC:556. AP1G2.

Subcellular locationi

Golgi apparatus membrane; Peripheral membrane protein. Cytoplasmic vesicle membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein
Note: Mainly localized to perinuclear vesicular structures. Colocalizes with HBV major surface antigen L and HBV core protein C in CD63-containing compartments. Colocalizes with HBV major surface antigen L to cis-Golgi-like structures.

GO - Cellular componenti

  1. AP-1 adaptor complex Source: UniProtKB
  2. endosome Source: UniProtKB-KW
  3. Golgi apparatus Source: ProtInc
  4. Golgi-associated vesicle Source: UniProtKB
  5. membrane Source: UniProtKB
  6. transport vesicle Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Endosome, Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi369 – 3691L → G: Greatly diminishes interaction with ubiquitin; when associated with G-372. 1 Publication
Mutagenesisi372 – 3721A → G: Greatly diminishes interaction with ubiquitin; when associated with G-369. 1 Publication
Mutagenesisi372 – 3721A → G: Greatly diminishes interaction with ubiquitin; when associated with G-376. 1 Publication
Mutagenesisi376 – 3761S → G: Greatly diminishes interaction with ubiquitin; when associated with G-372. 1 Publication

Organism-specific databases

PharmGKBiPA24846.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 785785AP-1 complex subunit gamma-like 2PRO_0000193760Add
BLAST

Proteomic databases

MaxQBiO75843.
PaxDbiO75843.
PeptideAtlasiO75843.
PRIDEiO75843.

PTM databases

PhosphoSiteiO75843.

Expressioni

Tissue specificityi

Expressed in all but one (skeletal muscle) tissues examined.

Gene expression databases

BgeeiO75843.
CleanExiHS_AP1G2.
ExpressionAtlasiO75843. baseline and differential.
GenevestigatoriO75843.

Organism-specific databases

HPAiHPA004106.

Interactioni

Subunit structurei

Probably interacts with AP1S1/Sigma1A-adaptin AP1S2/Sigma1B-adaptin. Probably does not interact with APB1. Interacts with HBV major surface antigen L. Interacts with HBV core protein C in a ubiquitin-dependent manner. Binds ubiquitin.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
RABEP1Q152762EBI-373637,EBI-447043

Protein-protein interaction databases

BioGridi114420. 17 interactions.
IntActiO75843. 12 interactions.
MINTiMINT-5005892.
STRINGi9606.ENSP00000312442.

Structurei

Secondary structure

1
785
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi670 – 6756Combined sources
Beta strandi678 – 6858Combined sources
Beta strandi693 – 70210Combined sources
Beta strandi704 – 7063Combined sources
Beta strandi708 – 7169Combined sources
Beta strandi721 – 7255Combined sources
Beta strandi731 – 7333Combined sources
Turni735 – 7373Combined sources
Beta strandi741 – 7488Combined sources
Beta strandi758 – 7658Combined sources
Beta strandi768 – 7758Combined sources
Helixi781 – 7833Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2E9GNMR-A662-785[»]
2YMTX-ray1.80A665-785[»]
3ZHFX-ray1.70A665-785[»]
4BCXX-ray2.00A665-785[»]
ProteinModelPortaliO75843.
SMRiO75843. Positions 6-589, 665-785.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO75843.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini665 – 780116GAEPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni369 – 37911Essential for ubiquitin-bindingAdd
BLAST

Sequence similaritiesi

Contains 1 GAE domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG303101.
GeneTreeiENSGT00390000012618.
HOGENOMiHOG000210271.
HOVERGENiHBG067473.
InParanoidiO75843.
KOiK12391.
OMAiPCAQLLH.
OrthoDBiEOG7S7SD7.
PhylomeDBiO75843.
TreeFamiTF300367.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
2.60.40.1230. 1 hit.
InterProiIPR017107. AP1_complex_gsu.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR002553. Clathrin/coatomer_adapt-like_N.
IPR008152. Clathrin_a/b/g-adaptin_app_Ig.
IPR008153. Clathrin_g-adaptin_app.
IPR013041. Coatomer/clathrin_app_Ig-like.
[Graphical view]
PfamiPF01602. Adaptin_N. 1 hit.
PF02883. Alpha_adaptinC2. 1 hit.
[Graphical view]
PIRSFiPIRSF037094. AP1_complex_gamma. 1 hit.
SMARTiSM00809. Alpha_adaptinC2. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
SSF49348. SSF49348. 1 hit.
PROSITEiPS50180. GAE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O75843-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVVPSLKLQD LIEEIRGAKT QAQEREVIQK ECAHIRASFR DGDPVHRHRQ
60 70 80 90 100
LAKLLYVHML GYPAHFGQME CLKLIASSRF TDKRVGYLGA MLLLDERHDA
110 120 130 140 150
HLLITNSIKN DLSQGIQPVQ GLALCTLSTM GSAEMCRDLA PEVEKLLLQP
160 170 180 190 200
SPYVRKKAIL TAVHMIRKVP ELSSVFLPPC AQLLHERHHG ILLGTITLIT
210 220 230 240 250
ELCERSPAAL RHFRKVVPQL VHILRTLVTM GYSTEHSISG VSDPFLQVQI
260 270 280 290 300
LRLLRILGRN HEESSETMND LLAQVATNTD TSRNAGNAVL FETVLTIMDI
310 320 330 340 350
RSAAGLRVLA VNILGRFLLN SDRNIRYVAL TSLLRLVQSD HSAVQRHRPT
360 370 380 390 400
VVECLRETDA SLSRRALELS LALVNSSNVR AMMQELQAFL ESCPPDLRAD
410 420 430 440 450
CASGILLAAE RFAPTKRWHI DTILHVLTTA GTHVRDDAVA NLTQLIGGAQ
460 470 480 490 500
ELHAYSVRRL YNALAEDISQ QPLVQVAAWC IGEYGDLLLA GNCEEIEPLQ
510 520 530 540 550
VDEEEVLALL EKVLQSHMSL PATRGYALTA LMKLSTRLCG DNNRIRQVVS
560 570 580 590 600
IYGSCLDVEL QQRAVEYDTL FRKYDHMRAA ILEKMPLVER DGPQADEEAK
610 620 630 640 650
ESKEAAQLSE AAPVPTEPQA SQLLDLLDLL DGASGDVQHP PHLDPSPGGA
660 670 680 690 700
LVHLLDLPCV PPPPAPIPDL KVFEREGVQL NLSFIRPPEN PALLLITITA
710 720 730 740 750
TNFSEGDVTH FICQAAVPKS LQLQLQAPSG NTVPARGGLP ITQLFRILNP
760 770 780
NKAPLRLKLR LTYDHFHQSV QEIFEVNNLP VESWQ
Length:785
Mass (Da):87,117
Last modified:November 1, 1998 - v1
Checksum:iC189192C9811111C
GO

Sequence cautioni

The sequence AAC67390.1 differs from that shown. Reason: Frameshift at position 713. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti204 – 2063ERS → GRN in AAC67390. (PubMed:9762922)Curated
Sequence conflicti399 – 3991A → C in AAC67390. (PubMed:9762922)Curated
Sequence conflicti402 – 4021A → D in AAC67390. (PubMed:9762922)Curated
Sequence conflicti416 – 4161K → T in AAC67390. (PubMed:9762922)Curated
Sequence conflicti429 – 4291T → S in AAC67390. (PubMed:9762922)Curated
Sequence conflicti439 – 4413VAN → AGHT in AAC67390. (PubMed:9762922)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti377 – 3771S → F.
Corresponds to variant rs12897422 [ dbSNP | Ensembl ].
VAR_024363

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB015318 mRNA. Translation: BAA33390.1.
AF068706 mRNA. Translation: AAC67390.1. Frameshift.
CH471078 Genomic DNA. Translation: EAW66132.1.
CH471078 Genomic DNA. Translation: EAW66134.1.
CH471078 Genomic DNA. Translation: EAW66138.1.
CCDSiCCDS9602.1.
RefSeqiNP_003908.1. NM_003917.4.
XP_005268229.1. XM_005268172.1.
XP_005268230.1. XM_005268173.1.
UniGeneiHs.343244.
Hs.569375.

Genome annotation databases

EnsembliENST00000308724; ENSP00000312442; ENSG00000213983.
ENST00000397120; ENSP00000380309; ENSG00000213983.
GeneIDi8906.
KEGGihsa:8906.
UCSCiuc001wkl.2. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB015318 mRNA. Translation: BAA33390.1 .
AF068706 mRNA. Translation: AAC67390.1 . Frameshift.
CH471078 Genomic DNA. Translation: EAW66132.1 .
CH471078 Genomic DNA. Translation: EAW66134.1 .
CH471078 Genomic DNA. Translation: EAW66138.1 .
CCDSi CCDS9602.1.
RefSeqi NP_003908.1. NM_003917.4.
XP_005268229.1. XM_005268172.1.
XP_005268230.1. XM_005268173.1.
UniGenei Hs.343244.
Hs.569375.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2E9G NMR - A 662-785 [» ]
2YMT X-ray 1.80 A 665-785 [» ]
3ZHF X-ray 1.70 A 665-785 [» ]
4BCX X-ray 2.00 A 665-785 [» ]
ProteinModelPortali O75843.
SMRi O75843. Positions 6-589, 665-785.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 114420. 17 interactions.
IntActi O75843. 12 interactions.
MINTi MINT-5005892.
STRINGi 9606.ENSP00000312442.

PTM databases

PhosphoSitei O75843.

Proteomic databases

MaxQBi O75843.
PaxDbi O75843.
PeptideAtlasi O75843.
PRIDEi O75843.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000308724 ; ENSP00000312442 ; ENSG00000213983 .
ENST00000397120 ; ENSP00000380309 ; ENSG00000213983 .
GeneIDi 8906.
KEGGi hsa:8906.
UCSCi uc001wkl.2. human.

Organism-specific databases

CTDi 8906.
GeneCardsi GC14M024028.
HGNCi HGNC:556. AP1G2.
HPAi HPA004106.
MIMi 603534. gene.
neXtProti NX_O75843.
PharmGKBi PA24846.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG303101.
GeneTreei ENSGT00390000012618.
HOGENOMi HOG000210271.
HOVERGENi HBG067473.
InParanoidi O75843.
KOi K12391.
OMAi PCAQLLH.
OrthoDBi EOG7S7SD7.
PhylomeDBi O75843.
TreeFami TF300367.

Miscellaneous databases

ChiTaRSi AP1G2. human.
EvolutionaryTracei O75843.
GeneWikii AP1G2.
GenomeRNAii 8906.
NextBioi 33461.
PROi O75843.
SOURCEi Search...

Gene expression databases

Bgeei O75843.
CleanExi HS_AP1G2.
ExpressionAtlasi O75843. baseline and differential.
Genevestigatori O75843.

Family and domain databases

Gene3Di 1.25.10.10. 1 hit.
2.60.40.1230. 1 hit.
InterProi IPR017107. AP1_complex_gsu.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR002553. Clathrin/coatomer_adapt-like_N.
IPR008152. Clathrin_a/b/g-adaptin_app_Ig.
IPR008153. Clathrin_g-adaptin_app.
IPR013041. Coatomer/clathrin_app_Ig-like.
[Graphical view ]
Pfami PF01602. Adaptin_N. 1 hit.
PF02883. Alpha_adaptinC2. 1 hit.
[Graphical view ]
PIRSFi PIRSF037094. AP1_complex_gamma. 1 hit.
SMARTi SM00809. Alpha_adaptinC2. 1 hit.
[Graphical view ]
SUPFAMi SSF48371. SSF48371. 1 hit.
SSF49348. SSF49348. 1 hit.
PROSITEi PS50180. GAE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and characterization of novel clathrin adaptor-related proteins."
    Takatsu H., Sakurai M., Shin H.-W., Murakami K., Nakayama K.
    J. Biol. Chem. 273:24693-24700(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, NEGATIVE INTERACTION WITH APB1, INTERACTION WITH AP1S1 AND AP1S2.
    Tissue: Liver.
  2. "Cloning, expression, and localization of a novel gamma-adaptin-like molecule."
    Lewin D.A., Sheff D., Ooi C.E., Whitney J.A., Yamamoto E., Chicione L.M., Webster P., Bonifacino J.S., Mellman I.
    FEBS Lett. 435:263-268(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "Hepatitis B virus large envelope protein interacts with gamma2-adaptin, a clathrin adaptor-related protein."
    Hartmann-Stuehler C., Prange R.
    J. Virol. 75:5343-5351(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH HBV MAJOR SURFACE ANTIGEN L, SUBCELLULAR LOCATION.
  5. "Gamma-adaptin, a novel ubiquitin-interacting adaptor, and Nedd4 ubiquitin ligase control hepatitis B virus maturation."
    Rost M., Mann S., Lambert C., Doring T., Thome N., Prange R.
    J. Biol. Chem. 281:29297-29308(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN HEPATITIS VIRUS B MATURATION, SUBCELLULAR LOCATION, INTERACTION WITH HBV CORE PROTEIN AND UBIQUITIN, MUTAGENESIS OF LEU-369; ALA-372 AND SER-376.
  6. "Hepatitis B virus maturation is sensitive to functional inhibition of ESCRT-III, Vps4, and gamma 2-adaptin."
    Lambert C., Doering T., Prange R.
    J. Virol. 81:9050-9060(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN HEPATITIS VIRUS B MATURATION.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Solution structure of the alpha adaptin C2 domain from human adapter-related protein complex 1 gamma 2 subunit."
    RIKEN structural genomics initiative (RSGI)
    Submitted (JUL-2007) to the PDB data bank
    Cited for: STRUCTURE BY NMR OF 662-785.

Entry informationi

Entry nameiAP1G2_HUMAN
AccessioniPrimary (citable) accession number: O75843
Secondary accession number(s): D3DS51, O75504
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: November 1, 1998
Last modified: November 26, 2014
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

Does not appear to be a subunit of the clathrin-associated adaptor protein complex 1 (AP-1).Curated

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3