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O75843

- AP1G2_HUMAN

UniProt

O75843 - AP1G2_HUMAN

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Protein
AP-1 complex subunit gamma-like 2
Gene
AP1G2
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

May function in protein sorting in late endosomes or multivesucular bodies (MVBs). Involved in MVB-assisted maturation of hepatitis B virus (HBV).3 Publications

GO - Molecular functioni

  1. protein binding Source: IntAct
  2. protein transporter activity Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. intracellular protein transport Source: InterPro
  2. vesicle-mediated transport Source: UniProtKB
  3. viral process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
AP-1 complex subunit gamma-like 2
Alternative name(s):
Gamma2-adaptin
Short name:
G2ad
Gene namesi
Name:AP1G2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 14

Organism-specific databases

HGNCiHGNC:556. AP1G2.

Subcellular locationi

Golgi apparatus membrane; Peripheral membrane protein. Cytoplasmic vesicle membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein
Note: Mainly localized to perinuclear vesicular structures. Colocalizes with HBV major surface antigen L and HBV core protein C in CD63-containing compartments. Colocalizes with HBV major surface antigen L to cis-Golgi-like structures.4 Publications

GO - Cellular componenti

  1. AP-1 adaptor complex Source: UniProtKB
  2. Golgi apparatus Source: ProtInc
  3. Golgi-associated vesicle Source: UniProtKB
  4. endosome membrane Source: UniProtKB-SubCell
  5. membrane Source: UniProtKB
  6. transport vesicle Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Endosome, Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi369 – 3691L → G: Greatly diminishes interaction with ubiquitin; when associated with G-372. 1 Publication
Mutagenesisi372 – 3721A → G: Greatly diminishes interaction with ubiquitin; when associated with G-369. 1 Publication
Mutagenesisi372 – 3721A → G: Greatly diminishes interaction with ubiquitin; when associated with G-376.
Mutagenesisi376 – 3761S → G: Greatly diminishes interaction with ubiquitin; when associated with G-372. 1 Publication

Organism-specific databases

PharmGKBiPA24846.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 785785AP-1 complex subunit gamma-like 2
PRO_0000193760Add
BLAST

Proteomic databases

MaxQBiO75843.
PaxDbiO75843.
PeptideAtlasiO75843.
PRIDEiO75843.

PTM databases

PhosphoSiteiO75843.

Expressioni

Tissue specificityi

Expressed in all but one (skeletal muscle) tissues examined.

Gene expression databases

ArrayExpressiO75843.
BgeeiO75843.
CleanExiHS_AP1G2.
GenevestigatoriO75843.

Organism-specific databases

HPAiHPA004106.

Interactioni

Subunit structurei

Probably interacts with AP1S1/Sigma1A-adaptin AP1S2/Sigma1B-adaptin. Probably does not interact with APB1. Interacts with HBV major surface antigen L. Interacts with HBV core protein C in a ubiquitin-dependent manner. Binds ubiquitin.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
RABEP1Q152762EBI-373637,EBI-447043

Protein-protein interaction databases

BioGridi114420. 13 interactions.
IntActiO75843. 12 interactions.
MINTiMINT-5005892.
STRINGi9606.ENSP00000312442.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi670 – 6756
Beta strandi678 – 6858
Beta strandi693 – 70210
Beta strandi704 – 7063
Beta strandi708 – 7169
Beta strandi721 – 7255
Beta strandi731 – 7333
Turni735 – 7373
Beta strandi741 – 7488
Beta strandi758 – 7658
Beta strandi768 – 7758
Helixi781 – 7833

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2E9GNMR-A662-785[»]
2YMTX-ray1.80A665-785[»]
3ZHFX-ray1.70A665-785[»]
4BCXX-ray2.00A665-785[»]
ProteinModelPortaliO75843.
SMRiO75843. Positions 8-586, 665-785.

Miscellaneous databases

EvolutionaryTraceiO75843.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini665 – 780116GAE
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni369 – 37911Essential for ubiquitin-binding
Add
BLAST

Sequence similaritiesi

Contains 1 GAE domain.

Phylogenomic databases

eggNOGiNOG303101.
HOGENOMiHOG000210271.
HOVERGENiHBG067473.
InParanoidiO75843.
KOiK12391.
OMAiPCAQLLH.
OrthoDBiEOG7S7SD7.
PhylomeDBiO75843.
TreeFamiTF300367.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
2.60.40.1230. 1 hit.
InterProiIPR017107. AP1_complex_gsu.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR002553. Clathrin/coatomer_adapt-like_N.
IPR008152. Clathrin_a/b/g-adaptin_app_Ig.
IPR008153. Clathrin_g-adaptin_app.
IPR013041. Coatomer/clathrin_app_Ig-like.
[Graphical view]
PfamiPF01602. Adaptin_N. 1 hit.
PF02883. Alpha_adaptinC2. 1 hit.
[Graphical view]
PIRSFiPIRSF037094. AP1_complex_gamma. 1 hit.
SMARTiSM00809. Alpha_adaptinC2. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
SSF49348. SSF49348. 1 hit.
PROSITEiPS50180. GAE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O75843-1 [UniParc]FASTAAdd to Basket

« Hide

MVVPSLKLQD LIEEIRGAKT QAQEREVIQK ECAHIRASFR DGDPVHRHRQ    50
LAKLLYVHML GYPAHFGQME CLKLIASSRF TDKRVGYLGA MLLLDERHDA 100
HLLITNSIKN DLSQGIQPVQ GLALCTLSTM GSAEMCRDLA PEVEKLLLQP 150
SPYVRKKAIL TAVHMIRKVP ELSSVFLPPC AQLLHERHHG ILLGTITLIT 200
ELCERSPAAL RHFRKVVPQL VHILRTLVTM GYSTEHSISG VSDPFLQVQI 250
LRLLRILGRN HEESSETMND LLAQVATNTD TSRNAGNAVL FETVLTIMDI 300
RSAAGLRVLA VNILGRFLLN SDRNIRYVAL TSLLRLVQSD HSAVQRHRPT 350
VVECLRETDA SLSRRALELS LALVNSSNVR AMMQELQAFL ESCPPDLRAD 400
CASGILLAAE RFAPTKRWHI DTILHVLTTA GTHVRDDAVA NLTQLIGGAQ 450
ELHAYSVRRL YNALAEDISQ QPLVQVAAWC IGEYGDLLLA GNCEEIEPLQ 500
VDEEEVLALL EKVLQSHMSL PATRGYALTA LMKLSTRLCG DNNRIRQVVS 550
IYGSCLDVEL QQRAVEYDTL FRKYDHMRAA ILEKMPLVER DGPQADEEAK 600
ESKEAAQLSE AAPVPTEPQA SQLLDLLDLL DGASGDVQHP PHLDPSPGGA 650
LVHLLDLPCV PPPPAPIPDL KVFEREGVQL NLSFIRPPEN PALLLITITA 700
TNFSEGDVTH FICQAAVPKS LQLQLQAPSG NTVPARGGLP ITQLFRILNP 750
NKAPLRLKLR LTYDHFHQSV QEIFEVNNLP VESWQ 785
Length:785
Mass (Da):87,117
Last modified:November 1, 1998 - v1
Checksum:iC189192C9811111C
GO

Sequence cautioni

The sequence AAC67390.1 differs from that shown. Reason: Frameshift at position 713.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti377 – 3771S → F.
Corresponds to variant rs12897422 [ dbSNP | Ensembl ].
VAR_024363

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti204 – 2063ERS → GRN in AAC67390. 1 Publication
Sequence conflicti399 – 3991A → C in AAC67390. 1 Publication
Sequence conflicti402 – 4021A → D in AAC67390. 1 Publication
Sequence conflicti416 – 4161K → T in AAC67390. 1 Publication
Sequence conflicti429 – 4291T → S in AAC67390. 1 Publication
Sequence conflicti439 – 4413VAN → AGHT in AAC67390. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB015318 mRNA. Translation: BAA33390.1.
AF068706 mRNA. Translation: AAC67390.1. Frameshift.
CH471078 Genomic DNA. Translation: EAW66132.1.
CH471078 Genomic DNA. Translation: EAW66134.1.
CH471078 Genomic DNA. Translation: EAW66138.1.
CCDSiCCDS9602.1.
RefSeqiNP_003908.1. NM_003917.4.
XP_005268229.1. XM_005268172.1.
XP_005268230.1. XM_005268173.1.
UniGeneiHs.343244.
Hs.569375.

Genome annotation databases

EnsembliENST00000308724; ENSP00000312442; ENSG00000213983.
ENST00000397120; ENSP00000380309; ENSG00000213983.
GeneIDi8906.
KEGGihsa:8906.
UCSCiuc001wkl.2. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB015318 mRNA. Translation: BAA33390.1 .
AF068706 mRNA. Translation: AAC67390.1 . Frameshift.
CH471078 Genomic DNA. Translation: EAW66132.1 .
CH471078 Genomic DNA. Translation: EAW66134.1 .
CH471078 Genomic DNA. Translation: EAW66138.1 .
CCDSi CCDS9602.1.
RefSeqi NP_003908.1. NM_003917.4.
XP_005268229.1. XM_005268172.1.
XP_005268230.1. XM_005268173.1.
UniGenei Hs.343244.
Hs.569375.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2E9G NMR - A 662-785 [» ]
2YMT X-ray 1.80 A 665-785 [» ]
3ZHF X-ray 1.70 A 665-785 [» ]
4BCX X-ray 2.00 A 665-785 [» ]
ProteinModelPortali O75843.
SMRi O75843. Positions 8-586, 665-785.
ModBasei Search...

Protein-protein interaction databases

BioGridi 114420. 13 interactions.
IntActi O75843. 12 interactions.
MINTi MINT-5005892.
STRINGi 9606.ENSP00000312442.

PTM databases

PhosphoSitei O75843.

Proteomic databases

MaxQBi O75843.
PaxDbi O75843.
PeptideAtlasi O75843.
PRIDEi O75843.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000308724 ; ENSP00000312442 ; ENSG00000213983 .
ENST00000397120 ; ENSP00000380309 ; ENSG00000213983 .
GeneIDi 8906.
KEGGi hsa:8906.
UCSCi uc001wkl.2. human.

Organism-specific databases

CTDi 8906.
GeneCardsi GC14M024028.
HGNCi HGNC:556. AP1G2.
HPAi HPA004106.
MIMi 603534. gene.
neXtProti NX_O75843.
PharmGKBi PA24846.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG303101.
HOGENOMi HOG000210271.
HOVERGENi HBG067473.
InParanoidi O75843.
KOi K12391.
OMAi PCAQLLH.
OrthoDBi EOG7S7SD7.
PhylomeDBi O75843.
TreeFami TF300367.

Miscellaneous databases

ChiTaRSi AP1G2. human.
EvolutionaryTracei O75843.
GeneWikii AP1G2.
GenomeRNAii 8906.
NextBioi 33461.
PROi O75843.
SOURCEi Search...

Gene expression databases

ArrayExpressi O75843.
Bgeei O75843.
CleanExi HS_AP1G2.
Genevestigatori O75843.

Family and domain databases

Gene3Di 1.25.10.10. 1 hit.
2.60.40.1230. 1 hit.
InterProi IPR017107. AP1_complex_gsu.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR002553. Clathrin/coatomer_adapt-like_N.
IPR008152. Clathrin_a/b/g-adaptin_app_Ig.
IPR008153. Clathrin_g-adaptin_app.
IPR013041. Coatomer/clathrin_app_Ig-like.
[Graphical view ]
Pfami PF01602. Adaptin_N. 1 hit.
PF02883. Alpha_adaptinC2. 1 hit.
[Graphical view ]
PIRSFi PIRSF037094. AP1_complex_gamma. 1 hit.
SMARTi SM00809. Alpha_adaptinC2. 1 hit.
[Graphical view ]
SUPFAMi SSF48371. SSF48371. 1 hit.
SSF49348. SSF49348. 1 hit.
PROSITEi PS50180. GAE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and characterization of novel clathrin adaptor-related proteins."
    Takatsu H., Sakurai M., Shin H.-W., Murakami K., Nakayama K.
    J. Biol. Chem. 273:24693-24700(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, NEGATIVE INTERACTION WITH APB1, INTERACTION WITH AP1S1 AND AP1S2.
    Tissue: Liver.
  2. "Cloning, expression, and localization of a novel gamma-adaptin-like molecule."
    Lewin D.A., Sheff D., Ooi C.E., Whitney J.A., Yamamoto E., Chicione L.M., Webster P., Bonifacino J.S., Mellman I.
    FEBS Lett. 435:263-268(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "Hepatitis B virus large envelope protein interacts with gamma2-adaptin, a clathrin adaptor-related protein."
    Hartmann-Stuehler C., Prange R.
    J. Virol. 75:5343-5351(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH HBV MAJOR SURFACE ANTIGEN L, SUBCELLULAR LOCATION.
  5. "Gamma-adaptin, a novel ubiquitin-interacting adaptor, and Nedd4 ubiquitin ligase control hepatitis B virus maturation."
    Rost M., Mann S., Lambert C., Doring T., Thome N., Prange R.
    J. Biol. Chem. 281:29297-29308(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN HEPATITIS VIRUS B MATURATION, SUBCELLULAR LOCATION, INTERACTION WITH HBV CORE PROTEIN AND UBIQUITIN, MUTAGENESIS OF LEU-369; ALA-372 AND SER-376.
  6. "Hepatitis B virus maturation is sensitive to functional inhibition of ESCRT-III, Vps4, and gamma 2-adaptin."
    Lambert C., Doering T., Prange R.
    J. Virol. 81:9050-9060(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN HEPATITIS VIRUS B MATURATION.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Solution structure of the alpha adaptin C2 domain from human adapter-related protein complex 1 gamma 2 subunit."
    RIKEN structural genomics initiative (RSGI)
    Submitted (JUL-2007) to the PDB data bank
    Cited for: STRUCTURE BY NMR OF 662-785.

Entry informationi

Entry nameiAP1G2_HUMAN
AccessioniPrimary (citable) accession number: O75843
Secondary accession number(s): D3DS51, O75504
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: November 1, 1998
Last modified: September 3, 2014
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

Does not appear to be a subunit of the clathrin-associated adaptor protein complex 1 (AP-1).

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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