Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

O75843

- AP1G2_HUMAN

UniProt

O75843 - AP1G2_HUMAN

Protein

AP-1 complex subunit gamma-like 2

Gene

AP1G2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 127 (01 Oct 2014)
      Sequence version 1 (01 Nov 1998)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    May function in protein sorting in late endosomes or multivesucular bodies (MVBs). Involved in MVB-assisted maturation of hepatitis B virus (HBV).3 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. protein transporter activity Source: InterPro

    GO - Biological processi

    1. intracellular protein transport Source: InterPro
    2. vesicle-mediated transport Source: UniProtKB
    3. viral process Source: UniProtKB-KW

    Keywords - Biological processi

    Host-virus interaction, Protein transport, Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    AP-1 complex subunit gamma-like 2
    Alternative name(s):
    Gamma2-adaptin
    Short name:
    G2ad
    Gene namesi
    Name:AP1G2
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 14

    Organism-specific databases

    HGNCiHGNC:556. AP1G2.

    Subcellular locationi

    Golgi apparatus membrane; Peripheral membrane protein. Cytoplasmic vesicle membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein
    Note: Mainly localized to perinuclear vesicular structures. Colocalizes with HBV major surface antigen L and HBV core protein C in CD63-containing compartments. Colocalizes with HBV major surface antigen L to cis-Golgi-like structures.

    GO - Cellular componenti

    1. AP-1 adaptor complex Source: UniProtKB
    2. endosome membrane Source: UniProtKB-SubCell
    3. Golgi apparatus Source: ProtInc
    4. Golgi-associated vesicle Source: UniProtKB
    5. membrane Source: UniProtKB
    6. transport vesicle Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasmic vesicle, Endosome, Golgi apparatus, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi369 – 3691L → G: Greatly diminishes interaction with ubiquitin; when associated with G-372. 1 Publication
    Mutagenesisi372 – 3721A → G: Greatly diminishes interaction with ubiquitin; when associated with G-369. 1 Publication
    Mutagenesisi372 – 3721A → G: Greatly diminishes interaction with ubiquitin; when associated with G-376. 1 Publication
    Mutagenesisi376 – 3761S → G: Greatly diminishes interaction with ubiquitin; when associated with G-372. 1 Publication

    Organism-specific databases

    PharmGKBiPA24846.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 785785AP-1 complex subunit gamma-like 2PRO_0000193760Add
    BLAST

    Proteomic databases

    MaxQBiO75843.
    PaxDbiO75843.
    PeptideAtlasiO75843.
    PRIDEiO75843.

    PTM databases

    PhosphoSiteiO75843.

    Expressioni

    Tissue specificityi

    Expressed in all but one (skeletal muscle) tissues examined.

    Gene expression databases

    ArrayExpressiO75843.
    BgeeiO75843.
    CleanExiHS_AP1G2.
    GenevestigatoriO75843.

    Organism-specific databases

    HPAiHPA004106.

    Interactioni

    Subunit structurei

    Probably interacts with AP1S1/Sigma1A-adaptin AP1S2/Sigma1B-adaptin. Probably does not interact with APB1. Interacts with HBV major surface antigen L. Interacts with HBV core protein C in a ubiquitin-dependent manner. Binds ubiquitin.3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    RABEP1Q152762EBI-373637,EBI-447043

    Protein-protein interaction databases

    BioGridi114420. 13 interactions.
    IntActiO75843. 12 interactions.
    MINTiMINT-5005892.
    STRINGi9606.ENSP00000312442.

    Structurei

    Secondary structure

    1
    785
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi670 – 6756
    Beta strandi678 – 6858
    Beta strandi693 – 70210
    Beta strandi704 – 7063
    Beta strandi708 – 7169
    Beta strandi721 – 7255
    Beta strandi731 – 7333
    Turni735 – 7373
    Beta strandi741 – 7488
    Beta strandi758 – 7658
    Beta strandi768 – 7758
    Helixi781 – 7833

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2E9GNMR-A662-785[»]
    2YMTX-ray1.80A665-785[»]
    3ZHFX-ray1.70A665-785[»]
    4BCXX-ray2.00A665-785[»]
    ProteinModelPortaliO75843.
    SMRiO75843. Positions 8-586, 665-785.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO75843.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini665 – 780116GAEPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni369 – 37911Essential for ubiquitin-bindingAdd
    BLAST

    Sequence similaritiesi

    Contains 1 GAE domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG303101.
    HOGENOMiHOG000210271.
    HOVERGENiHBG067473.
    InParanoidiO75843.
    KOiK12391.
    OMAiPCAQLLH.
    OrthoDBiEOG7S7SD7.
    PhylomeDBiO75843.
    TreeFamiTF300367.

    Family and domain databases

    Gene3Di1.25.10.10. 1 hit.
    2.60.40.1230. 1 hit.
    InterProiIPR017107. AP1_complex_gsu.
    IPR011989. ARM-like.
    IPR016024. ARM-type_fold.
    IPR002553. Clathrin/coatomer_adapt-like_N.
    IPR008152. Clathrin_a/b/g-adaptin_app_Ig.
    IPR008153. Clathrin_g-adaptin_app.
    IPR013041. Coatomer/clathrin_app_Ig-like.
    [Graphical view]
    PfamiPF01602. Adaptin_N. 1 hit.
    PF02883. Alpha_adaptinC2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF037094. AP1_complex_gamma. 1 hit.
    SMARTiSM00809. Alpha_adaptinC2. 1 hit.
    [Graphical view]
    SUPFAMiSSF48371. SSF48371. 1 hit.
    SSF49348. SSF49348. 1 hit.
    PROSITEiPS50180. GAE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O75843-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVVPSLKLQD LIEEIRGAKT QAQEREVIQK ECAHIRASFR DGDPVHRHRQ    50
    LAKLLYVHML GYPAHFGQME CLKLIASSRF TDKRVGYLGA MLLLDERHDA 100
    HLLITNSIKN DLSQGIQPVQ GLALCTLSTM GSAEMCRDLA PEVEKLLLQP 150
    SPYVRKKAIL TAVHMIRKVP ELSSVFLPPC AQLLHERHHG ILLGTITLIT 200
    ELCERSPAAL RHFRKVVPQL VHILRTLVTM GYSTEHSISG VSDPFLQVQI 250
    LRLLRILGRN HEESSETMND LLAQVATNTD TSRNAGNAVL FETVLTIMDI 300
    RSAAGLRVLA VNILGRFLLN SDRNIRYVAL TSLLRLVQSD HSAVQRHRPT 350
    VVECLRETDA SLSRRALELS LALVNSSNVR AMMQELQAFL ESCPPDLRAD 400
    CASGILLAAE RFAPTKRWHI DTILHVLTTA GTHVRDDAVA NLTQLIGGAQ 450
    ELHAYSVRRL YNALAEDISQ QPLVQVAAWC IGEYGDLLLA GNCEEIEPLQ 500
    VDEEEVLALL EKVLQSHMSL PATRGYALTA LMKLSTRLCG DNNRIRQVVS 550
    IYGSCLDVEL QQRAVEYDTL FRKYDHMRAA ILEKMPLVER DGPQADEEAK 600
    ESKEAAQLSE AAPVPTEPQA SQLLDLLDLL DGASGDVQHP PHLDPSPGGA 650
    LVHLLDLPCV PPPPAPIPDL KVFEREGVQL NLSFIRPPEN PALLLITITA 700
    TNFSEGDVTH FICQAAVPKS LQLQLQAPSG NTVPARGGLP ITQLFRILNP 750
    NKAPLRLKLR LTYDHFHQSV QEIFEVNNLP VESWQ 785
    Length:785
    Mass (Da):87,117
    Last modified:November 1, 1998 - v1
    Checksum:iC189192C9811111C
    GO

    Sequence cautioni

    The sequence AAC67390.1 differs from that shown. Reason: Frameshift at position 713.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti204 – 2063ERS → GRN in AAC67390. (PubMed:9762922)Curated
    Sequence conflicti399 – 3991A → C in AAC67390. (PubMed:9762922)Curated
    Sequence conflicti402 – 4021A → D in AAC67390. (PubMed:9762922)Curated
    Sequence conflicti416 – 4161K → T in AAC67390. (PubMed:9762922)Curated
    Sequence conflicti429 – 4291T → S in AAC67390. (PubMed:9762922)Curated
    Sequence conflicti439 – 4413VAN → AGHT in AAC67390. (PubMed:9762922)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti377 – 3771S → F.
    Corresponds to variant rs12897422 [ dbSNP | Ensembl ].
    VAR_024363

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB015318 mRNA. Translation: BAA33390.1.
    AF068706 mRNA. Translation: AAC67390.1. Frameshift.
    CH471078 Genomic DNA. Translation: EAW66132.1.
    CH471078 Genomic DNA. Translation: EAW66134.1.
    CH471078 Genomic DNA. Translation: EAW66138.1.
    CCDSiCCDS9602.1.
    RefSeqiNP_003908.1. NM_003917.4.
    XP_005268229.1. XM_005268172.1.
    XP_005268230.1. XM_005268173.1.
    UniGeneiHs.343244.
    Hs.569375.

    Genome annotation databases

    EnsembliENST00000308724; ENSP00000312442; ENSG00000213983.
    ENST00000397120; ENSP00000380309; ENSG00000213983.
    GeneIDi8906.
    KEGGihsa:8906.
    UCSCiuc001wkl.2. human.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB015318 mRNA. Translation: BAA33390.1 .
    AF068706 mRNA. Translation: AAC67390.1 . Frameshift.
    CH471078 Genomic DNA. Translation: EAW66132.1 .
    CH471078 Genomic DNA. Translation: EAW66134.1 .
    CH471078 Genomic DNA. Translation: EAW66138.1 .
    CCDSi CCDS9602.1.
    RefSeqi NP_003908.1. NM_003917.4.
    XP_005268229.1. XM_005268172.1.
    XP_005268230.1. XM_005268173.1.
    UniGenei Hs.343244.
    Hs.569375.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2E9G NMR - A 662-785 [» ]
    2YMT X-ray 1.80 A 665-785 [» ]
    3ZHF X-ray 1.70 A 665-785 [» ]
    4BCX X-ray 2.00 A 665-785 [» ]
    ProteinModelPortali O75843.
    SMRi O75843. Positions 8-586, 665-785.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 114420. 13 interactions.
    IntActi O75843. 12 interactions.
    MINTi MINT-5005892.
    STRINGi 9606.ENSP00000312442.

    PTM databases

    PhosphoSitei O75843.

    Proteomic databases

    MaxQBi O75843.
    PaxDbi O75843.
    PeptideAtlasi O75843.
    PRIDEi O75843.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000308724 ; ENSP00000312442 ; ENSG00000213983 .
    ENST00000397120 ; ENSP00000380309 ; ENSG00000213983 .
    GeneIDi 8906.
    KEGGi hsa:8906.
    UCSCi uc001wkl.2. human.

    Organism-specific databases

    CTDi 8906.
    GeneCardsi GC14M024028.
    HGNCi HGNC:556. AP1G2.
    HPAi HPA004106.
    MIMi 603534. gene.
    neXtProti NX_O75843.
    PharmGKBi PA24846.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG303101.
    HOGENOMi HOG000210271.
    HOVERGENi HBG067473.
    InParanoidi O75843.
    KOi K12391.
    OMAi PCAQLLH.
    OrthoDBi EOG7S7SD7.
    PhylomeDBi O75843.
    TreeFami TF300367.

    Miscellaneous databases

    ChiTaRSi AP1G2. human.
    EvolutionaryTracei O75843.
    GeneWikii AP1G2.
    GenomeRNAii 8906.
    NextBioi 33461.
    PROi O75843.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O75843.
    Bgeei O75843.
    CleanExi HS_AP1G2.
    Genevestigatori O75843.

    Family and domain databases

    Gene3Di 1.25.10.10. 1 hit.
    2.60.40.1230. 1 hit.
    InterProi IPR017107. AP1_complex_gsu.
    IPR011989. ARM-like.
    IPR016024. ARM-type_fold.
    IPR002553. Clathrin/coatomer_adapt-like_N.
    IPR008152. Clathrin_a/b/g-adaptin_app_Ig.
    IPR008153. Clathrin_g-adaptin_app.
    IPR013041. Coatomer/clathrin_app_Ig-like.
    [Graphical view ]
    Pfami PF01602. Adaptin_N. 1 hit.
    PF02883. Alpha_adaptinC2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF037094. AP1_complex_gamma. 1 hit.
    SMARTi SM00809. Alpha_adaptinC2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48371. SSF48371. 1 hit.
    SSF49348. SSF49348. 1 hit.
    PROSITEi PS50180. GAE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification and characterization of novel clathrin adaptor-related proteins."
      Takatsu H., Sakurai M., Shin H.-W., Murakami K., Nakayama K.
      J. Biol. Chem. 273:24693-24700(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, NEGATIVE INTERACTION WITH APB1, INTERACTION WITH AP1S1 AND AP1S2.
      Tissue: Liver.
    2. "Cloning, expression, and localization of a novel gamma-adaptin-like molecule."
      Lewin D.A., Sheff D., Ooi C.E., Whitney J.A., Yamamoto E., Chicione L.M., Webster P., Bonifacino J.S., Mellman I.
      FEBS Lett. 435:263-268(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "Hepatitis B virus large envelope protein interacts with gamma2-adaptin, a clathrin adaptor-related protein."
      Hartmann-Stuehler C., Prange R.
      J. Virol. 75:5343-5351(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH HBV MAJOR SURFACE ANTIGEN L, SUBCELLULAR LOCATION.
    5. "Gamma-adaptin, a novel ubiquitin-interacting adaptor, and Nedd4 ubiquitin ligase control hepatitis B virus maturation."
      Rost M., Mann S., Lambert C., Doring T., Thome N., Prange R.
      J. Biol. Chem. 281:29297-29308(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN HEPATITIS VIRUS B MATURATION, SUBCELLULAR LOCATION, INTERACTION WITH HBV CORE PROTEIN AND UBIQUITIN, MUTAGENESIS OF LEU-369; ALA-372 AND SER-376.
    6. "Hepatitis B virus maturation is sensitive to functional inhibition of ESCRT-III, Vps4, and gamma 2-adaptin."
      Lambert C., Doering T., Prange R.
      J. Virol. 81:9050-9060(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN HEPATITIS VIRUS B MATURATION.
    7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "Solution structure of the alpha adaptin C2 domain from human adapter-related protein complex 1 gamma 2 subunit."
      RIKEN structural genomics initiative (RSGI)
      Submitted (JUL-2007) to the PDB data bank
      Cited for: STRUCTURE BY NMR OF 662-785.

    Entry informationi

    Entry nameiAP1G2_HUMAN
    AccessioniPrimary (citable) accession number: O75843
    Secondary accession number(s): D3DS51, O75504
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 24, 2001
    Last sequence update: November 1, 1998
    Last modified: October 1, 2014
    This is version 127 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Caution

    Does not appear to be a subunit of the clathrin-associated adaptor protein complex 1 (AP-1).Curated

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 14
      Human chromosome 14: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3