O75841 (UPK1B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uroplakin-1b
Short name=UP1b

Alternative name(s):
Tetraspanin-20
Short name=Tspan-20
Uroplakin Ib
Short name=UPIb

Gene names

Name:	UPK1B
Synonyms:	TSPAN20

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	260 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Component of the asymmetric unit membrane (AUM); a highly specialized biomembrane elaborated by terminally differentiated urothelial cells. May play an important role in normal bladder epithelial physiology, possibly in regulating membrane permeability of superficial umbrella cells or in stabilizing the apical membrane through AUM/cytoskeletal interactions By similarity.
Subunit structure	Heterodimer with uroplakin-3A (UPK3A) or uroplakin-3B (UPK3B) By similarity.
Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	Bladder epithelium.
Post-translational modification	N-glycosylated with high-mannose oligosaccharides By similarity.
Sequence similarities	Belongs to the tetraspanin (TM4SF) family.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane Transmembrane helix
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	epithelial cell differentiation Inferred from direct assay PubMed 10514386. Source: UniProtKB
Cellular_component	apical plasma membrane Inferred from electronic annotation. Source: Compara integral to membrane Non-traceable author statement Ref.1Ref.2. Source: UniProtKB
Molecular_function	structural molecule activity Non-traceable author statement Ref.2. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 260	259	Uroplakin-1b		PRO_0000219289
Regions
	Topological domain	2 – 15	14	Cytoplasmic Potential
	Transmembrane	16 – 36	21	Helical; Potential
	Topological domain	37 – 60	24	Extracellular Potential
	Transmembrane	61 – 81	21	Helical; Potential
	Topological domain	82 – 86	5	Cytoplasmic Potential
	Transmembrane	87 – 107	21	Helical; Potential
	Topological domain	108 – 229	122	Extracellular Potential
	Transmembrane	230 – 250	21	Helical; Potential
	Topological domain	251 – 260	10	Cytoplasmic Potential
Natural variations
	Natural variant	113	1	Q → R. Ref.1 Ref.2 Ref.5 Ref.6 Ref.7 Corresponds to variant rs9840317 [ dbSNP | Ensembl ].		VAR_028780
Experimental info
	Sequence conflict	137	1	N → D in BAA33724. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O75841 [UniParc]. Last modified November 30, 2010. Version 5. Checksum: 0512AF8D33884C0F	FASTA	260	29,643
	10 20 30 40 50 60 MAKDNSTVRC FQGLLIFGNV IIGCCGIALT AECIFFVSDQ HSLYPLLEAT DNDDIYGAAW 70 80 90 100 110 120 IGIFVGICLF CLSVLGIVGI MKSSRKILLA YFILMFIVYA FEVASCITAA TQQDFFTPNL 130 140 150 160 170 180 FLKQMLERYQ NNSPPNNDDQ WKNNGVTKTW DRLMLQDNCC GVNGPSDWQK YTSAFRTENN 190 200 210 220 230 240 DADYPWPRQC CVMNNLKEPL NLEACKLGVP GFYHNQGCYE LISGPMNRHA WGVAWFGFAI 250 260 LCWTFWVLLG TMFYWSRIEY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Expression of uroplakin Ib and uroplakin III genes in tissues and peripheral blood of patients with transitional cell carcinoma." Yuasa T., Yoshiki T., Tanaka T., Kim C.J., Isono T., Okada Y. Jpn. J. Cancer Res. 89:879-882(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ARG-113. Tissue: Urinary bladder urothelium.
[2]	"Cloning of the human uroplakin 1B cDNA and analysis of its expression in urothelial-tumor cell lines and bladder-carcinoma tissue." Finch J.L., Miller J., Aspinall J.O., Cowled P.A. Int. J. Cancer 80:533-538(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ARG-113. Tissue: Urothelium.
[3]	"Molecular cloning of human uroplakin Ib cDNA." Adachi W., Nishida K., Kinoshita S., Matsubara K., Okubo K. Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Corneal epithelium.
[4]	"The DNA sequence, annotation and analysis of human chromosome 3." Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J. , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A. Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ARG-113. Tissue: Ovary.
[6]	"Uroplakin gene expression by normal and neoplastic human urothelium." Lobban E.D., Smith B.A., Hall G.D., Harnden P., Roberts P., Selby P.J., Trejdosiewicz L.K., Southgate J. Am. J. Pathol. 153:1957-1967(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 11-252, VARIANT ARG-113.
[7]	"Chromosomal localization of the human urothelial 'tetraspan' gene, UPK1B, to 3q13.3-q21 and detection of a TaqI polymorphism." Finch J.L., Webb G.C., Evdokiou A., Cowled P.A. Genomics 40:501-503(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 103-142, VARIANT ARG-113.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB015234 mRNA. Translation: BAA33724.1. AF042331 mRNA. Translation: AAC77794.1. AB002155 mRNA. Translation: BAA88878.1. AC083800 Genomic DNA. No translation available. BC063568 mRNA. Translation: AAH63568.1. AF082888 mRNA. Translation: AAD09401.1. AF067147 Genomic DNA. Translation: AAC17446.1.
IPI	IPI00215889.
RefSeq	NP_008883.2. NM_006952.3.
UniGene	Hs.271580.
3D structure databases
ProteinModelPortal	O75841.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000264234.
Proteomic databases
PaxDb	O75841.
PRIDE	O75841.
Protocols and materials databases
DNASU	7348.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000264234; ENSP00000264234; ENSG00000114638.
GeneID	7348.
KEGG	hsa:7348.
UCSC	uc003ecc.3. human.
Organism-specific databases
CTD	7348.
GeneCards	GC03P118892.
HGNC	HGNC:12578. UPK1B.
HPA	HPA031799. HPA031800.
MIM	602380. gene.
neXtProt	NX_O75841.
PharmGKB	PA37210.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG247872.
HOGENOM	HOG000230653.
HOVERGEN	HBG054305.
InParanoid	O75841.
OMA	QGCYELI.
OrthoDB	EOG4RNB8Z.
PhylomeDB	O75841.
Gene expression databases
ArrayExpress	O75841.
Bgee	O75841.
CleanEx	HS_UPK1B.
Genevestigator	O75841.
GermOnline	ENSG00000114638. Homo sapiens.
Family and domain databases
InterPro	IPR000301. Tetraspanin. IPR018499. Tetraspanin/Peripherin. IPR008952. Tetraspanin_EC2. [Graphical view]
Pfam	PF00335. Tetraspannin. 1 hit. [Graphical view]
PRINTS	PR00259. TMFOUR.
SUPFAM	SSF48652. Tetraspanin. 1 hit.
ProtoNet	Search...
Other
ChiTaRS	UPK1B. human.
GenomeRNAi	7348.
NextBio	28766.
SOURCE	Search...

Entry information

Entry name

UPK1B_HUMAN

Accession

Primary (citable) accession number: O75841
Secondary accession number(s): O60753, Q9UIM2, Q9UNX6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	November 30, 2010
Last modified:	April 3, 2013
This is version 101 of the entry and version 5 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.