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Protein

Carbonyl reductase [NADPH] 3

Gene

CBR3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Has low NADPH-dependent oxidoreductase activity towards 4-benzoylpyridine and menadione (in vitro).1 Publication

Catalytic activityi

R-CHOH-R' + NADP+ = R-CO-R' + NADPH.

Kineticsi

  1. KM=25 µM for menadione2 Publications
  2. KM=43 µM for menadione2 Publications
  3. KM=90 µM for NADPH2 Publications
  4. KM=38 µM for NADPH2 Publications

    pH dependencei

    Optimum pH is 5.5-7.2 Publications

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei90NADP; via carbonyl oxygen1 Publication1
    Binding sitei140SubstrateBy similarity1
    Active sitei194Proton acceptorPROSITE-ProRule annotation1
    Binding sitei239NADP1 Publication1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi10 – 34NADP1 PublicationAdd BLAST25
    Nucleotide bindingi38 – 42NADP1 Publication5
    Nucleotide bindingi63 – 64NADP1 Publication2
    Nucleotide bindingi194 – 198NADP1 Publication5

    GO - Molecular functioni

    • 3-keto sterol reductase activity Source: Ensembl
    • carbonyl reductase (NADPH) activity Source: UniProtKB
    • NADPH binding Source: UniProtKB

    GO - Biological processi

    • cognition Source: UniProtKB
    • phylloquinone catabolic process Source: Ensembl
    • xenobiotic metabolic process Source: Reactome

    Keywordsi

    Molecular functionOxidoreductase
    LigandNADP

    Enzyme and pathway databases

    BRENDAi1.1.1.184 2681
    ReactomeiR-HSA-211945 Phase I - Functionalization of compounds
    SABIO-RKiO75828

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Carbonyl reductase [NADPH] 3 (EC:1.1.1.184)
    Alternative name(s):
    NADPH-dependent carbonyl reductase 3
    Short chain dehydrogenase/reductase family 21C member 2
    Gene namesi
    Name:CBR3
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    Proteomesi
    • UP000005640 Componenti: Chromosome 21

    Organism-specific databases

    EuPathDBiHostDB:ENSG00000159231.5
    HGNCiHGNC:1549 CBR3
    MIMi603608 gene
    neXtProtiNX_O75828

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Organism-specific databases

    DisGeNETi874
    OpenTargetsiENSG00000159231
    PharmGKBiPA26122

    Chemistry databases

    ChEMBLiCHEMBL6008
    DrugBankiDB00997 Doxorubicin

    Polymorphism and mutation databases

    BioMutaiCBR3

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Initiator methionineiRemovedBy similarity
    ChainiPRO_00000546082 – 277Carbonyl reductase [NADPH] 3Add BLAST276

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei2N-acetylserineBy similarity1
    Modified residuei30PhosphoserineBy similarity1

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    EPDiO75828
    PaxDbiO75828
    PeptideAtlasiO75828
    PRIDEiO75828

    2D gel databases

    REPRODUCTION-2DPAGEiIPI00290462

    PTM databases

    iPTMnetiO75828
    PhosphoSitePlusiO75828
    SwissPalmiO75828

    Expressioni

    Tissue specificityi

    Detected in ovary, pancreas, intestine, colon, kidney, brain, thymus, lung, heart, liver, spleen, leukocyte, prostate and testis.1 Publication

    Gene expression databases

    BgeeiENSG00000159231
    CleanExiHS_CBR3
    ExpressionAtlasiO75828 baseline and differential
    GenevisibleiO75828 HS

    Organism-specific databases

    HPAiHPA018434

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    SDCBPO005605EBI-714504,EBI-727004

    Protein-protein interaction databases

    BioGridi107320, 17 interactors
    IntActiO75828, 13 interactors
    STRINGi9606.ENSP00000290354

    Structurei

    Secondary structure

    1277
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi7 – 12Combined sources6
    Helixi16 – 28Combined sources13
    Beta strandi30 – 39Combined sources10
    Helixi40 – 52Combined sources13
    Beta strandi58 – 61Combined sources4
    Helixi67 – 81Combined sources15
    Beta strandi82 – 89Combined sources8
    Helixi103 – 114Combined sources12
    Helixi116 – 125Combined sources10
    Helixi126 – 128Combined sources3
    Beta strandi129 – 138Combined sources10
    Helixi142 – 149Combined sources8
    Helixi152 – 159Combined sources8
    Helixi165 – 180Combined sources16
    Turni184 – 188Combined sources5
    Helixi193 – 215Combined sources23
    Helixi217 – 219Combined sources3
    Beta strandi222 – 227Combined sources6
    Beta strandi237 – 239Combined sources3
    Helixi241 – 247Combined sources7
    Helixi249 – 255Combined sources7
    Beta strandi268 – 270Combined sources3
    Beta strandi273 – 275Combined sources3

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2HRBX-ray1.90A6-277[»]
    ProteinModelPortaliO75828
    SMRiO75828
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO75828

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiKOG1208 Eukaryota
    COG1028 LUCA
    GeneTreeiENSGT00510000046499
    HOVERGENiHBG001909
    InParanoidiO75828
    KOiK00084
    OMAiNEMDTNS
    OrthoDBiEOG091G0V7L
    PhylomeDBiO75828
    TreeFamiTF329359

    Family and domain databases

    InterProiView protein in InterPro
    IPR036291 NAD(P)-bd_dom_sf
    IPR020904 Sc_DH/Rdtase_CS
    IPR002347 SDR_fam
    PfamiView protein in Pfam
    PF00106 adh_short, 2 hits
    PRINTSiPR00081 GDHRDH
    PR00080 SDRFAMILY
    SUPFAMiSSF51735 SSF51735, 1 hit
    PROSITEiView protein in PROSITE
    PS00061 ADH_SHORT, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O75828-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSSCSRVALV TGANRGIGLA IARELCRQFS GDVVLTARDV ARGQAAVQQL
    60 70 80 90 100
    QAEGLSPRFH QLDIDDLQSI RALRDFLRKE YGGLNVLVNN AAVAFKSDDP
    110 120 130 140 150
    MPFDIKAEMT LKTNFFATRN MCNELLPIMK PHGRVVNISS LQCLRAFENC
    160 170 180 190 200
    SEDLQERFHS ETLTEGDLVD LMKKFVEDTK NEVHEREGWP NSPYGVSKLG
    210 220 230 240 250
    VTVLSRILAR RLDEKRKADR ILVNACCPGP VKTDMDGKDS IRTVEEGAET
    260 270
    PVYLALLPPD ATEPQGQLVH DKVVQNW
    Length:277
    Mass (Da):30,850
    Last modified:January 23, 2007 - v3
    Checksum:i8D39D6B99CFE5389
    GO

    Natural variant

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Natural variantiVAR_0338684C → Y1 PublicationCorresponds to variant dbSNP:rs8133052Ensembl.1
    Natural variantiVAR_03386984L → V1 PublicationCorresponds to variant dbSNP:rs9282628Ensembl.1
    Natural variantiVAR_03387093V → I. Corresponds to variant dbSNP:rs2835285Ensembl.1
    Natural variantiVAR_033871131P → S1 PublicationCorresponds to variant dbSNP:rs16993929Ensembl.1
    Natural variantiVAR_033872235M → L1 PublicationCorresponds to variant dbSNP:rs4987121Ensembl.1
    Natural variantiVAR_033873244V → M Increased catalytic activity. 2 PublicationsCorresponds to variant dbSNP:rs1056892Ensembl.1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB004854 mRNA Translation: BAA33500.1
    AB003151 Genomic DNA Translation: BAA34207.1
    AB041012 mRNA Translation: BAD74062.1
    AB124847 mRNA Translation: BAE45939.1
    CR541709 mRNA Translation: CAG46510.1
    EF462915 Genomic DNA Translation: ABO43035.1
    AP000689 Genomic DNA Translation: BAA89425.1
    AP001725 Genomic DNA Translation: BAA95547.1
    CH471079 Genomic DNA Translation: EAX09747.1
    BC002812 mRNA Translation: AAH02812.1
    CCDSiCCDS13642.1
    RefSeqiNP_001227.1, NM_001236.3
    UniGeneiHs.154510

    Genome annotation databases

    EnsembliENST00000290354; ENSP00000290354; ENSG00000159231
    GeneIDi874
    KEGGihsa:874

    Keywords - Coding sequence diversityi

    Polymorphism

    Similar proteinsi

    Entry informationi

    Entry nameiCBR3_HUMAN
    AccessioniPrimary (citable) accession number: O75828
    Secondary accession number(s): Q6FHP2
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: January 23, 2007
    Last modified: March 28, 2018
    This is version 163 of the entry and version 3 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health