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Protein

Carbonyl reductase [NADPH] 3

Gene

CBR3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has low NADPH-dependent oxidoreductase activity towards 4-benzoylpyridine and menadione (in vitro).1 Publication

Catalytic activityi

R-CHOH-R' + NADP+ = R-CO-R' + NADPH.

Kineticsi

  1. KM=25 µM for menadione2 Publications
  2. KM=43 µM for menadione2 Publications
  3. KM=90 µM for NADPH2 Publications
  4. KM=38 µM for NADPH2 Publications

    pH dependencei

    Optimum pH is 5.5-7.2 Publications

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei90NADP; via carbonyl oxygen1 Publication1
    Binding sitei140SubstrateBy similarity1
    Active sitei194Proton acceptorPROSITE-ProRule annotation1
    Binding sitei239NADP1 Publication1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi10 – 34NADP1 PublicationAdd BLAST25
    Nucleotide bindingi38 – 42NADP1 Publication5
    Nucleotide bindingi63 – 64NADP1 Publication2
    Nucleotide bindingi194 – 198NADP1 Publication5

    GO - Molecular functioni

    • 3-keto sterol reductase activity Source: Ensembl
    • carbonyl reductase (NADPH) activity Source: UniProtKB
    • NADPH binding Source: UniProtKB

    GO - Biological processi

    • cognition Source: UniProtKB
    • phylloquinone catabolic process Source: Ensembl
    Complete GO annotation...

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    BioCyciZFISH:HS08379-MONOMER.
    BRENDAi1.1.1.184. 2681.
    SABIO-RKO75828.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Carbonyl reductase [NADPH] 3 (EC:1.1.1.184)
    Alternative name(s):
    NADPH-dependent carbonyl reductase 3
    Short chain dehydrogenase/reductase family 21C member 2
    Gene namesi
    Name:CBR3
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    Proteomesi
    • UP000005640 Componenti: Chromosome 21

    Organism-specific databases

    HGNCiHGNC:1549. CBR3.

    Subcellular locationi

    • Cytoplasm 1 Publication

    GO - Cellular componenti

    • cytoplasm Source: HPA
    • cytosol Source: UniProtKB
    • extracellular space Source: UniProtKB
    • nucleoplasm Source: HPA
    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Organism-specific databases

    DisGeNETi874.
    OpenTargetsiENSG00000159231.
    PharmGKBiPA26122.

    Chemistry databases

    ChEMBLiCHEMBL6008.
    DrugBankiDB00997. Doxorubicin.

    Polymorphism and mutation databases

    BioMutaiCBR3.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Initiator methionineiRemovedBy similarity
    ChainiPRO_00000546082 – 277Carbonyl reductase [NADPH] 3Add BLAST276

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei2N-acetylserineBy similarity1
    Modified residuei2PhosphoserineBy similarity1
    Modified residuei30PhosphoserineBy similarity1
    Modified residuei162PhosphothreonineBy similarity1

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    EPDiO75828.
    PaxDbiO75828.
    PeptideAtlasiO75828.
    PRIDEiO75828.

    2D gel databases

    REPRODUCTION-2DPAGEIPI00290462.

    PTM databases

    iPTMnetiO75828.
    PhosphoSitePlusiO75828.
    SwissPalmiO75828.

    Expressioni

    Tissue specificityi

    Detected in ovary, pancreas, intestine, colon, kidney, brain, thymus, lung, heart, liver, spleen, leukocyte, prostate and testis.1 Publication

    Gene expression databases

    BgeeiENSG00000159231.
    CleanExiHS_CBR3.
    ExpressionAtlasiO75828. baseline and differential.
    GenevisibleiO75828. HS.

    Organism-specific databases

    HPAiHPA018434.

    Interactioni

    Protein-protein interaction databases

    BioGridi107320. 17 interactors.
    IntActiO75828. 11 interactors.
    MINTiMINT-1412792.
    STRINGi9606.ENSP00000290354.

    Structurei

    Secondary structure

    1277
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi7 – 12Combined sources6
    Helixi16 – 28Combined sources13
    Beta strandi30 – 39Combined sources10
    Helixi40 – 52Combined sources13
    Beta strandi58 – 61Combined sources4
    Helixi67 – 81Combined sources15
    Beta strandi82 – 89Combined sources8
    Helixi103 – 114Combined sources12
    Helixi116 – 125Combined sources10
    Helixi126 – 128Combined sources3
    Beta strandi129 – 138Combined sources10
    Helixi142 – 149Combined sources8
    Helixi152 – 159Combined sources8
    Helixi165 – 180Combined sources16
    Turni184 – 188Combined sources5
    Helixi193 – 215Combined sources23
    Helixi217 – 219Combined sources3
    Beta strandi222 – 227Combined sources6
    Beta strandi237 – 239Combined sources3
    Helixi241 – 247Combined sources7
    Helixi249 – 255Combined sources7
    Beta strandi268 – 270Combined sources3
    Beta strandi273 – 275Combined sources3

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2HRBX-ray1.90A6-277[»]
    ProteinModelPortaliO75828.
    SMRiO75828.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO75828.

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiKOG1208. Eukaryota.
    COG1028. LUCA.
    GeneTreeiENSGT00510000046499.
    HOVERGENiHBG001909.
    InParanoidiO75828.
    KOiK00084.
    OMAiTENSMEN.
    OrthoDBiEOG091G0V7L.
    PhylomeDBiO75828.
    TreeFamiTF329359.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR016040. NAD(P)-bd_dom.
    IPR020904. Sc_DH/Rdtase_CS.
    IPR002347. SDR_fam.
    [Graphical view]
    PANTHERiPTHR24322. PTHR24322. 2 hits.
    PfamiPF00106. adh_short. 2 hits.
    [Graphical view]
    PRINTSiPR00081. GDHRDH.
    PR00080. SDRFAMILY.
    SUPFAMiSSF51735. SSF51735. 1 hit.
    PROSITEiPS00061. ADH_SHORT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O75828-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSSCSRVALV TGANRGIGLA IARELCRQFS GDVVLTARDV ARGQAAVQQL
    60 70 80 90 100
    QAEGLSPRFH QLDIDDLQSI RALRDFLRKE YGGLNVLVNN AAVAFKSDDP
    110 120 130 140 150
    MPFDIKAEMT LKTNFFATRN MCNELLPIMK PHGRVVNISS LQCLRAFENC
    160 170 180 190 200
    SEDLQERFHS ETLTEGDLVD LMKKFVEDTK NEVHEREGWP NSPYGVSKLG
    210 220 230 240 250
    VTVLSRILAR RLDEKRKADR ILVNACCPGP VKTDMDGKDS IRTVEEGAET
    260 270
    PVYLALLPPD ATEPQGQLVH DKVVQNW
    Length:277
    Mass (Da):30,850
    Last modified:January 23, 2007 - v3
    Checksum:i8D39D6B99CFE5389
    GO

    Natural variant

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Natural variantiVAR_0338684C → Y.1 PublicationCorresponds to variant rs8133052dbSNPEnsembl.1
    Natural variantiVAR_03386984L → V.1 PublicationCorresponds to variant rs9282628dbSNPEnsembl.1
    Natural variantiVAR_03387093V → I.Corresponds to variant rs2835285dbSNPEnsembl.1
    Natural variantiVAR_033871131P → S.1 PublicationCorresponds to variant rs16993929dbSNPEnsembl.1
    Natural variantiVAR_033872235M → L.1 PublicationCorresponds to variant rs4987121dbSNPEnsembl.1
    Natural variantiVAR_033873244V → M Increased catalytic activity. 2 PublicationsCorresponds to variant rs1056892dbSNPEnsembl.1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB004854 mRNA. Translation: BAA33500.1.
    AB003151 Genomic DNA. Translation: BAA34207.1.
    AB041012 mRNA. Translation: BAD74062.1.
    AB124847 mRNA. Translation: BAE45939.1.
    CR541709 mRNA. Translation: CAG46510.1.
    EF462915 Genomic DNA. Translation: ABO43035.1.
    AP000689 Genomic DNA. Translation: BAA89425.1.
    AP001725 Genomic DNA. Translation: BAA95547.1.
    CH471079 Genomic DNA. Translation: EAX09747.1.
    BC002812 mRNA. Translation: AAH02812.1.
    CCDSiCCDS13642.1.
    RefSeqiNP_001227.1. NM_001236.3.
    UniGeneiHs.154510.

    Genome annotation databases

    EnsembliENST00000290354; ENSP00000290354; ENSG00000159231.
    GeneIDi874.
    KEGGihsa:874.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Web resourcesi

    SeattleSNPs

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB004854 mRNA. Translation: BAA33500.1.
    AB003151 Genomic DNA. Translation: BAA34207.1.
    AB041012 mRNA. Translation: BAD74062.1.
    AB124847 mRNA. Translation: BAE45939.1.
    CR541709 mRNA. Translation: CAG46510.1.
    EF462915 Genomic DNA. Translation: ABO43035.1.
    AP000689 Genomic DNA. Translation: BAA89425.1.
    AP001725 Genomic DNA. Translation: BAA95547.1.
    CH471079 Genomic DNA. Translation: EAX09747.1.
    BC002812 mRNA. Translation: AAH02812.1.
    CCDSiCCDS13642.1.
    RefSeqiNP_001227.1. NM_001236.3.
    UniGeneiHs.154510.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2HRBX-ray1.90A6-277[»]
    ProteinModelPortaliO75828.
    SMRiO75828.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi107320. 17 interactors.
    IntActiO75828. 11 interactors.
    MINTiMINT-1412792.
    STRINGi9606.ENSP00000290354.

    Chemistry databases

    ChEMBLiCHEMBL6008.
    DrugBankiDB00997. Doxorubicin.

    PTM databases

    iPTMnetiO75828.
    PhosphoSitePlusiO75828.
    SwissPalmiO75828.

    Polymorphism and mutation databases

    BioMutaiCBR3.

    2D gel databases

    REPRODUCTION-2DPAGEIPI00290462.

    Proteomic databases

    EPDiO75828.
    PaxDbiO75828.
    PeptideAtlasiO75828.
    PRIDEiO75828.

    Protocols and materials databases

    DNASUi874.
    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENST00000290354; ENSP00000290354; ENSG00000159231.
    GeneIDi874.
    KEGGihsa:874.

    Organism-specific databases

    CTDi874.
    DisGeNETi874.
    GeneCardsiCBR3.
    HGNCiHGNC:1549. CBR3.
    HPAiHPA018434.
    MIMi603608. gene.
    neXtProtiNX_O75828.
    OpenTargetsiENSG00000159231.
    PharmGKBiPA26122.
    GenAtlasiSearch...

    Phylogenomic databases

    eggNOGiKOG1208. Eukaryota.
    COG1028. LUCA.
    GeneTreeiENSGT00510000046499.
    HOVERGENiHBG001909.
    InParanoidiO75828.
    KOiK00084.
    OMAiTENSMEN.
    OrthoDBiEOG091G0V7L.
    PhylomeDBiO75828.
    TreeFamiTF329359.

    Enzyme and pathway databases

    BioCyciZFISH:HS08379-MONOMER.
    BRENDAi1.1.1.184. 2681.
    SABIO-RKO75828.

    Miscellaneous databases

    EvolutionaryTraceiO75828.
    GeneWikiiCBR3.
    GenomeRNAii874.
    PROiO75828.
    SOURCEiSearch...

    Gene expression databases

    BgeeiENSG00000159231.
    CleanExiHS_CBR3.
    ExpressionAtlasiO75828. baseline and differential.
    GenevisibleiO75828. HS.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR016040. NAD(P)-bd_dom.
    IPR020904. Sc_DH/Rdtase_CS.
    IPR002347. SDR_fam.
    [Graphical view]
    PANTHERiPTHR24322. PTHR24322. 2 hits.
    PfamiPF00106. adh_short. 2 hits.
    [Graphical view]
    PRINTSiPR00081. GDHRDH.
    PR00080. SDRFAMILY.
    SUPFAMiSSF51735. SSF51735. 1 hit.
    PROSITEiPS00061. ADH_SHORT. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiCBR3_HUMAN
    AccessioniPrimary (citable) accession number: O75828
    Secondary accession number(s): Q6FHP2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: January 23, 2007
    Last modified: November 30, 2016
    This is version 154 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 21
      Human chromosome 21: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.