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Protein

Breast cancer anti-estrogen resistance protein 3

Gene

BCAR3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May act as an adapter protein and couple activated growth factor receptors to a signaling pathway that regulates the proliferation in breast cancer cells. When overexpressed, it confers anti-estrogen resistance in breast cancer cell lines. May also be regulated by cellular adhesion to extracellular matrix proteins.1 Publication

GO - Molecular functioni

GO - Biological processi

  • lens morphogenesis in camera-type eye Source: Ensembl
  • positive regulation of peptidyl-serine phosphorylation Source: Ensembl
  • response to drug Source: ProtInc
  • signal transduction Source: ProtInc
  • small GTPase mediated signal transduction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Enzyme and pathway databases

BioCyciZFISH:ENSG00000137936-MONOMER.
SignaLinkiO75815.

Names & Taxonomyi

Protein namesi
Recommended name:
Breast cancer anti-estrogen resistance protein 3
Alternative name(s):
Novel SH2-containing protein 2
SH2 domain-containing protein 3B
Gene namesi
Name:BCAR3
Synonyms:NSP2, SH2D3B
ORF Names:UNQ271/PRO308
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:973. BCAR3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

DisGeNETi8412.
OpenTargetsiENSG00000137936.
PharmGKBiPA25283.

Polymorphism and mutation databases

BioMutaiBCAR3.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002302842 – 825Breast cancer anti-estrogen resistance protein 3Add BLAST824

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei32PhosphoserineCombined sources1
Modified residuei78PhosphoserineCombined sources1
Modified residuei83PhosphoserineCombined sources1
Modified residuei182PhosphoserineCombined sources1
Modified residuei290PhosphoserineCombined sources1
Modified residuei334N6-methyllysineCombined sources1
Modified residuei358PhosphoserineCombined sources1
Modified residuei363PhosphoserineCombined sources1
Modified residuei375PhosphoserineCombined sources1
Modified residuei442Omega-N-methylarginineCombined sources1
Modified residuei471PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

EPDiO75815.
MaxQBiO75815.
PaxDbiO75815.
PeptideAtlasiO75815.
PRIDEiO75815.

PTM databases

iPTMnetiO75815.
PhosphoSitePlusiO75815.

Expressioni

Tissue specificityi

Ubiquitously expressed. Found in several cancer cell lines, but not in nonmalignant breast tissue.2 Publications

Gene expression databases

BgeeiENSG00000137936.
CleanExiHS_BCAR3.
GenevisibleiO75815. HS.

Organism-specific databases

HPAiHPA014858.

Interactioni

Subunit structurei

Interacts with BCAR1, NEDD9, PTK2/FAK1 and PTPN1.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
ERBB2P046262EBI-702336,EBI-641062
IRS1P355683EBI-702336,EBI-517592
KITP107213EBI-702336,EBI-1379503
NEDD9Q145113EBI-702336,EBI-2108053

GO - Molecular functioni

Protein-protein interaction databases

BioGridi114000. 37 interactors.
DIPiDIP-33857N.
IntActiO75815. 36 interactors.
STRINGi9606.ENSP00000260502.

Structurei

Secondary structure

1825
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi517 – 519Combined sources3
Helixi534 – 546Combined sources13
Helixi549 – 563Combined sources15
Helixi571 – 577Combined sources7
Beta strandi578 – 580Combined sources3
Helixi582 – 585Combined sources4
Helixi589 – 591Combined sources3
Helixi592 – 614Combined sources23
Helixi620 – 639Combined sources20
Helixi644 – 655Combined sources12
Helixi657 – 660Combined sources4
Helixi663 – 672Combined sources10
Helixi674 – 682Combined sources9
Helixi684 – 692Combined sources9
Helixi696 – 698Combined sources3
Helixi711 – 717Combined sources7
Helixi724 – 726Combined sources3
Helixi732 – 734Combined sources3
Helixi737 – 752Combined sources16
Helixi754 – 764Combined sources11
Turni765 – 767Combined sources3
Helixi772 – 777Combined sources6
Helixi780 – 792Combined sources13
Helixi797 – 815Combined sources19

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3T6AX-ray2.40A/B/C/D502-825[»]
ProteinModelPortaliO75815.
SMRiO75815.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini154 – 253SH2PROSITE-ProRule annotationAdd BLAST100
Domaini548 – 818Ras-GEFPROSITE-ProRule annotationAdd BLAST271

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi479 – 482Poly-Asp4

Sequence similaritiesi

Contains 1 Ras-GEF domain.PROSITE-ProRule annotation
Contains 1 SH2 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH2 domain

Phylogenomic databases

eggNOGiENOG410IFQG. Eukaryota.
ENOG410XTJR. LUCA.
GeneTreeiENSGT00390000008976.
HOGENOMiHOG000231595.
HOVERGENiHBG053174.
InParanoidiO75815.
OMAiLPYGHQL.
OrthoDBiEOG091G01KG.
PhylomeDBiO75815.
TreeFamiTF323756.

Family and domain databases

CDDicd00155. RasGEF. 1 hit.
Gene3Di1.10.840.10. 1 hit.
3.30.505.10. 1 hit.
InterProiIPR028849. BCAR3.
IPR023578. Ras_GEF_dom.
IPR001895. RASGEF_cat_dom.
IPR000980. SH2.
[Graphical view]
PANTHERiPTHR14247:SF10. PTHR14247:SF10. 1 hit.
PfamiPF00617. RasGEF. 1 hit.
PF00017. SH2. 1 hit.
[Graphical view]
SMARTiSM00147. RasGEF. 1 hit.
SM00252. SH2. 1 hit.
[Graphical view]
SUPFAMiSSF48366. SSF48366. 1 hit.
SSF55550. SSF55550. 1 hit.
PROSITEiPS50009. RASGEF_CAT. 1 hit.
PS50001. SH2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O75815-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAGKFASLP RNMPVNHQFP LASSMDLLSS RSPLAEHRPD AYQDVSIHGT
60 70 80 90 100
LPRKKKGPPP IRSCDDFSHM GTLPHSKSPR QNSPVTQDGI QESPWQDRHG
110 120 130 140 150
ETFTFRDPHL LDPTVEYVKF SKERHIMDRT PEKLKKELEE ELLLSSEDLR
160 170 180 190 200
SHAWYHGRIP RQVSENLVQR DGDFLVRDSL SSPGNFVLTC QWKNLAQHFK
210 220 230 240 250
INRTVLRLSE AYSRVQYQFE MESFDSIPGL VRCYVGNRRP ISQQSGAIIF
260 270 280 290 300
QPINRTVPLR CLEEHYGTSP GQAREGSLTK GRPDVAKRLS LTMGGVQARE
310 320 330 340 350
QNLPRGNLLR NKEKSGSQPA CLDHMQDRRA LSLKAHQSES YLPIGCKLPP
360 370 380 390 400
QSSGVDTSPC PNSPVFRTGS EPALSPAVVR RVSSDARAGE ALRGSDSQLC
410 420 430 440 450
PKPPPKPCKV PFLKVPSSPS AWLNSEANYC ELNPAFATGC GRGAKLPSCA
460 470 480 490 500
QGSHTELLTA KQNEAPGPRN SGVNYLILDD DDRERPWEPA AAQMEKGQWD
510 520 530 540 550
KGEFVTPLLE TVSSFRPNEF ESKFLPPENK PLETAMLKRA KELFTNNDPK
560 570 580 590 600
VIAQHVLSMD CRVARILGVS EEMRRNMGVS SGLELITLPH GHQLRLDIIE
610 620 630 640 650
RHNTMAIGIA VDILGCTGTL EDRAATLSKI IQVAVELKDS MGDLYSFSAL
660 670 680 690 700
MKALEMPQIT RLEKTWTALR HQYTQTAILY EKQLKPFSKL LHEGRESTCV
710 720 730 740 750
PPNNVSVPLL MPLVTLMERQ AVTFEGTDMW EKNDQSCEIM LNHLATARFM
760 770 780 790 800
AEAADSYRMN AERILAGFQP DEEMNEICKT EFQMRLLWGS KGAQVNQTER
810 820
YEKFNQILTA LSRKLEPPPV KQAEL
Length:825
Mass (Da):92,566
Last modified:November 1, 1998 - v1
Checksum:iC55D61BB4AC7D0F2
GO
Isoform 2 (identifier: O75815-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-324: Missing.

Note: No experimental confirmation available.
Show »
Length:501
Mass (Da):55,745
Checksum:i50F3B53FB4BAF59E
GO
Isoform 3 (identifier: O75815-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-91: Missing.
     92-117: ESPWQDRHGETFTFRDPHLLDPTVEY → MPKECSAFHALSAALCCFYHRKSFIG

Note: No experimental confirmation available.
Show »
Length:734
Mass (Da):82,357
Checksum:iF48AF61CA484420A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti519E → G in AL833121 (PubMed:17974005).Curated1
Sequence conflicti543L → P in AL833121 (PubMed:17974005).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_050689464E → G.Corresponds to variant rs12062278dbSNPEnsembl.1
Natural variantiVAR_050690593Q → H.Corresponds to variant rs17110107dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0178141 – 324Missing in isoform 2. 1 PublicationAdd BLAST324
Alternative sequenceiVSP_0467161 – 91Missing in isoform 3. 1 PublicationAdd BLAST91
Alternative sequenceiVSP_04671792 – 117ESPWQ…PTVEY → MPKECSAFHALSAALCCFYH RKSFIG in isoform 3. 1 PublicationAdd BLAST26

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U92715 mRNA. Translation: AAC39777.1.
AF124250 mRNA. Translation: AAD28245.1.
AY358996 mRNA. Translation: AAQ89355.1.
AL833121 mRNA. No translation available.
AL049796 Genomic DNA. No translation available.
AL109613, AL359820 Genomic DNA. Translation: CAI18950.1.
AL359820, AL109613 Genomic DNA. Translation: CAH73975.1.
AL512488 Genomic DNA. No translation available.
CH471097 Genomic DNA. Translation: EAW73065.1.
CH471097 Genomic DNA. Translation: EAW73066.1.
CH471097 Genomic DNA. Translation: EAW73068.1.
BC039895 mRNA. Translation: AAH39895.1.
CCDSiCCDS58010.1. [O75815-3]
CCDS745.1. [O75815-1]
CCDS76181.1. [O75815-2]
RefSeqiNP_001248337.1. NM_001261408.1. [O75815-1]
NP_001248338.1. NM_001261409.1. [O75815-1]
NP_001248339.1. NM_001261410.1. [O75815-3]
NP_001295180.1. NM_001308251.1. [O75815-2]
NP_003558.1. NM_003567.3. [O75815-1]
XP_016857969.1. XM_017002480.1. [O75815-1]
UniGeneiHs.36958.

Genome annotation databases

EnsembliENST00000260502; ENSP00000260502; ENSG00000137936. [O75815-1]
ENST00000370243; ENSP00000359263; ENSG00000137936. [O75815-1]
ENST00000370244; ENSP00000359264; ENSG00000137936. [O75815-1]
ENST00000370247; ENSP00000359267; ENSG00000137936. [O75815-3]
ENST00000539242; ENSP00000441343; ENSG00000137936. [O75815-2]
GeneIDi8412.
KEGGihsa:8412.
UCSCiuc001dpx.6. human. [O75815-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U92715 mRNA. Translation: AAC39777.1.
AF124250 mRNA. Translation: AAD28245.1.
AY358996 mRNA. Translation: AAQ89355.1.
AL833121 mRNA. No translation available.
AL049796 Genomic DNA. No translation available.
AL109613, AL359820 Genomic DNA. Translation: CAI18950.1.
AL359820, AL109613 Genomic DNA. Translation: CAH73975.1.
AL512488 Genomic DNA. No translation available.
CH471097 Genomic DNA. Translation: EAW73065.1.
CH471097 Genomic DNA. Translation: EAW73066.1.
CH471097 Genomic DNA. Translation: EAW73068.1.
BC039895 mRNA. Translation: AAH39895.1.
CCDSiCCDS58010.1. [O75815-3]
CCDS745.1. [O75815-1]
CCDS76181.1. [O75815-2]
RefSeqiNP_001248337.1. NM_001261408.1. [O75815-1]
NP_001248338.1. NM_001261409.1. [O75815-1]
NP_001248339.1. NM_001261410.1. [O75815-3]
NP_001295180.1. NM_001308251.1. [O75815-2]
NP_003558.1. NM_003567.3. [O75815-1]
XP_016857969.1. XM_017002480.1. [O75815-1]
UniGeneiHs.36958.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3T6AX-ray2.40A/B/C/D502-825[»]
ProteinModelPortaliO75815.
SMRiO75815.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114000. 37 interactors.
DIPiDIP-33857N.
IntActiO75815. 36 interactors.
STRINGi9606.ENSP00000260502.

PTM databases

iPTMnetiO75815.
PhosphoSitePlusiO75815.

Polymorphism and mutation databases

BioMutaiBCAR3.

Proteomic databases

EPDiO75815.
MaxQBiO75815.
PaxDbiO75815.
PeptideAtlasiO75815.
PRIDEiO75815.

Protocols and materials databases

DNASUi8412.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000260502; ENSP00000260502; ENSG00000137936. [O75815-1]
ENST00000370243; ENSP00000359263; ENSG00000137936. [O75815-1]
ENST00000370244; ENSP00000359264; ENSG00000137936. [O75815-1]
ENST00000370247; ENSP00000359267; ENSG00000137936. [O75815-3]
ENST00000539242; ENSP00000441343; ENSG00000137936. [O75815-2]
GeneIDi8412.
KEGGihsa:8412.
UCSCiuc001dpx.6. human. [O75815-1]

Organism-specific databases

CTDi8412.
DisGeNETi8412.
GeneCardsiBCAR3.
HGNCiHGNC:973. BCAR3.
HPAiHPA014858.
MIMi604704. gene.
neXtProtiNX_O75815.
OpenTargetsiENSG00000137936.
PharmGKBiPA25283.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IFQG. Eukaryota.
ENOG410XTJR. LUCA.
GeneTreeiENSGT00390000008976.
HOGENOMiHOG000231595.
HOVERGENiHBG053174.
InParanoidiO75815.
OMAiLPYGHQL.
OrthoDBiEOG091G01KG.
PhylomeDBiO75815.
TreeFamiTF323756.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000137936-MONOMER.
SignaLinkiO75815.

Miscellaneous databases

ChiTaRSiBCAR3. human.
GeneWikiiBCAR3.
GenomeRNAii8412.
PROiO75815.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000137936.
CleanExiHS_BCAR3.
GenevisibleiO75815. HS.

Family and domain databases

CDDicd00155. RasGEF. 1 hit.
Gene3Di1.10.840.10. 1 hit.
3.30.505.10. 1 hit.
InterProiIPR028849. BCAR3.
IPR023578. Ras_GEF_dom.
IPR001895. RASGEF_cat_dom.
IPR000980. SH2.
[Graphical view]
PANTHERiPTHR14247:SF10. PTHR14247:SF10. 1 hit.
PfamiPF00617. RasGEF. 1 hit.
PF00017. SH2. 1 hit.
[Graphical view]
SMARTiSM00147. RasGEF. 1 hit.
SM00252. SH2. 1 hit.
[Graphical view]
SUPFAMiSSF48366. SSF48366. 1 hit.
SSF55550. SSF55550. 1 hit.
PROSITEiPS50009. RASGEF_CAT. 1 hit.
PS50001. SH2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBCAR3_HUMAN
AccessioniPrimary (citable) accession number: O75815
Secondary accession number(s): D3DT43
, Q5TEW3, Q6UW40, Q9BR50
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: November 1, 1998
Last modified: November 30, 2016
This is version 142 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.