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Protein

Calpain-15

Gene

CAPN15

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei552 – 5521By similarity
Active sitei717 – 7171By similarity
Active sitei737 – 7371By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri3 – 3230RanBP2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri44 – 7330RanBP2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri143 – 17230RanBP2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri338 – 36932RanBP2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri412 – 44130RanBP2-type 5PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. calcium-dependent cysteine-type endopeptidase activity Source: GO_Central
  2. cysteine-type peptidase activity Source: ProtInc
  3. peptidase activity Source: ProtInc
  4. sequence-specific DNA binding transcription factor activity Source: ProtInc
  5. zinc ion binding Source: InterPro

GO - Biological processi

  1. proteolysis Source: GO_Central
  2. regulation of transcription, DNA-templated Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiC02.010.

Names & Taxonomyi

Protein namesi
Recommended name:
Calpain-15 (EC:3.4.22.-)
Alternative name(s):
Small optic lobes homolog
Gene namesi
Name:CAPN15
Synonyms:SOLH
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:11182. CAPN15.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: GO_Central
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA36019.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10861086Calpain-15PRO_0000278770Add
BLAST

Proteomic databases

MaxQBiO75808.
PaxDbiO75808.
PRIDEiO75808.

PTM databases

PhosphoSiteiO75808.

Expressioni

Tissue specificityi

Widely expressed with higher expression in brain.1 Publication

Gene expression databases

BgeeiO75808.
CleanExiHS_SOLH.
ExpressionAtlasiO75808. baseline and differential.
GenevestigatoriO75808.

Organism-specific databases

HPAiHPA011960.

Interactioni

Protein-protein interaction databases

BioGridi112533. 4 interactions.
IntActiO75808. 1 interaction.
STRINGi9606.ENSP00000219611.

Structurei

3D structure databases

ProteinModelPortaliO75808.
SMRiO75808. Positions 474-898.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini487 – 793307Calpain catalyticPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi124 – 14219Glu-richAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C2 family.Curated
Contains 1 calpain catalytic domain.PROSITE-ProRule annotation
Contains 5 RanBP2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri3 – 3230RanBP2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri44 – 7330RanBP2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri143 – 17230RanBP2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri338 – 36932RanBP2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri412 – 44130RanBP2-type 5PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiNOG327523.
GeneTreeiENSGT00750000117668.
HOGENOMiHOG000007650.
HOVERGENiHBG080984.
InParanoidiO75808.
KOiK08582.
OMAiHGFQEHS.
OrthoDBiEOG7NKKJJ.
PhylomeDBiO75808.
TreeFamiTF322245.

Family and domain databases

InterProiIPR022684. Calpain_cysteine_protease.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
IPR001876. Znf_RanBP2.
[Graphical view]
PfamiPF00648. Peptidase_C2. 1 hit.
PF00641. zf-RanBP. 3 hits.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00230. CysPc. 1 hit.
SM00547. ZnF_RBZ. 5 hits.
[Graphical view]
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
PS01358. ZF_RANBP2_1. 5 hits.
PS50199. ZF_RANBP2_2. 4 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O75808-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATVGEWSCV RCTFLNPAGQ RQCSICEAPR HKPDLNHILR LSVEEQKWPC
60 70 80 90 100
ARCTFRNFLG KEACEVCGFT PEPAPGAAFL PVLNGVLPKP PAILGEPKGS
110 120 130 140 150
CQEEAGPVRT AGLVATEPAR GQCEDKDEEE KEEQEEEEGA AEPRGGWACP
160 170 180 190 200
RCTLHNTPVA SSCSVCGGPR RLSLPRIPPE ALVVPEVVAP AGFHVVPAAP
210 220 230 240 250
PPGLPGEGAE ANPPATSQGP AAEPEPPRVP PFSPFSSTLQ NNPVPRSRRE
260 270 280 290 300
VPPQLQPPVP EAAQPSPSAG CRGAPQGSGW AGASRLAELL SGKRLSVLEE
310 320 330 340 350
EATEGGTSRV EAGSSTSGSD IIDLAGDTVR YTPASPSSPD FTTWSCAKCT
360 370 380 390 400
LRNPTVAPRC SACGCSKLHG FQEHGEPPTH CPDCGADKPS PCGRSCGRVS
410 420 430 440 450
SAQKAARVLP ERPGQWACPA CTLLNALRAK HCAACHTPQL LVAQRRGAAP
460 470 480 490 500
LRRRESMHVE QRRQTDEGEA KALWENIVAF CRENNVSFVD DSFPPGPESV
510 520 530 540 550
GFPAGDSVQQ RVRQWLRPQE INCSVFRDHR ATWSVFHTLR PSDILQGLLG
560 570 580 590 600
NCWFLSALAV LAERPDLVER VMVTRSLCAE GAYQVRLCKD GTWTTVLVDD
610 620 630 640 650
MLPCDEAGCL LFSQAQRKQL WVALIEKALA KLHGSYFALQ AGRAIEGLAT
660 670 680 690 700
LTGAPCESLA LQLSSTNPRE EPVDTDLIWA KMLSSKEAGF LMGASCGGGN
710 720 730 740 750
MKVDDSAYES LGLRPRHAYS ILDVRDVQGT RLLRLRNPWG RFSWNGSWSD
760 770 780 790 800
EWPHWPGHLR GELMPHGSSE GVFWMEYGDF VRYFDSVDIC KVHSDWQEAR
810 820 830 840 850
VQGCFPSSAS APVGVTALTV LERASLEFAL FQEGSRRSDA VDSHLLDLCI
860 870 880 890 900
LVFRATFGSG GHLSLGRLLA HSKRAVKKFV SCDVMLEPGE YAVVCCAFNH
910 920 930 940 950
WGPPLPGTPA PQASSPSAGV PRASPEPPGH VLAVYSSRLV MVEPVEAQPT
960 970 980 990 1000
TLADAIILLT ESRGERHEGR EGMTCYYLTH GWAGLIVVVE NRHPKAYLHV
1010 1020 1030 1040 1050
QCDCTDSFNV VSTRGSLRTQ DSVPPLHRQV LVILSQLEGN AGFSITHRLA
1060 1070 1080
HRKAAQAFLS DWTASKGTHS PPLTPEVAGL HGPRPL
Length:1,086
Mass (Da):117,314
Last modified:October 31, 1998 - v1
Checksum:i9809A5CF9134A12C
GO
Isoform 2 (identifier: O75808-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     355-415: TVAPRCSACG...ARVLPERPGQ → CPPGLQPLGR...REPRRAARGP
     416-1086: Missing.

Note: No experimental confirmation available.

Show »
Length:415
Mass (Da):43,366
Checksum:i49992E1FBEE9704A
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei355 – 41561TVAPR…ERPGQ → CPPGLQPLGRGPESLPRAAG PRAGCVQLEAGHGGARGSPA DHAGRRHHPAHREPRRAARG P in isoform 2. 1 PublicationVSP_023361Add
BLAST
Alternative sequencei416 – 1086671Missing in isoform 2. 1 PublicationVSP_023362Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U85647 mRNA. Translation: AAC33822.1.
AE006464 Genomic DNA. Translation: AAK61233.1.
Z97986 Genomic DNA. Translation: CAC14067.1.
CH471112 Genomic DNA. Translation: EAW85803.1.
BC001869 mRNA. Translation: AAH01869.1.
BC021854 mRNA. Translation: AAH21854.1.
BC112918 mRNA. Translation: AAI12919.1.
CCDSiCCDS10410.1. [O75808-1]
RefSeqiNP_005623.1. NM_005632.2. [O75808-1]
XP_005255573.1. XM_005255516.2. [O75808-1]
XP_005255574.1. XM_005255517.2. [O75808-1]
XP_005255575.1. XM_005255518.2. [O75808-1]
UniGeneiHs.632219.

Genome annotation databases

EnsembliENST00000219611; ENSP00000219611; ENSG00000103326. [O75808-1]
GeneIDi6650.
KEGGihsa:6650.
UCSCiuc002chh.1. human. [O75808-2]
uc002chi.3. human. [O75808-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U85647 mRNA. Translation: AAC33822.1.
AE006464 Genomic DNA. Translation: AAK61233.1.
Z97986 Genomic DNA. Translation: CAC14067.1.
CH471112 Genomic DNA. Translation: EAW85803.1.
BC001869 mRNA. Translation: AAH01869.1.
BC021854 mRNA. Translation: AAH21854.1.
BC112918 mRNA. Translation: AAI12919.1.
CCDSiCCDS10410.1. [O75808-1]
RefSeqiNP_005623.1. NM_005632.2. [O75808-1]
XP_005255573.1. XM_005255516.2. [O75808-1]
XP_005255574.1. XM_005255517.2. [O75808-1]
XP_005255575.1. XM_005255518.2. [O75808-1]
UniGeneiHs.632219.

3D structure databases

ProteinModelPortaliO75808.
SMRiO75808. Positions 474-898.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112533. 4 interactions.
IntActiO75808. 1 interaction.
STRINGi9606.ENSP00000219611.

Protein family/group databases

MEROPSiC02.010.

PTM databases

PhosphoSiteiO75808.

Proteomic databases

MaxQBiO75808.
PaxDbiO75808.
PRIDEiO75808.

Protocols and materials databases

DNASUi6650.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000219611; ENSP00000219611; ENSG00000103326. [O75808-1]
GeneIDi6650.
KEGGihsa:6650.
UCSCiuc002chh.1. human. [O75808-2]
uc002chi.3. human. [O75808-1]

Organism-specific databases

CTDi6650.
GeneCardsiGC16P000578.
HGNCiHGNC:11182. CAPN15.
HPAiHPA011960.
MIMi603267. gene.
neXtProtiNX_O75808.
PharmGKBiPA36019.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG327523.
GeneTreeiENSGT00750000117668.
HOGENOMiHOG000007650.
HOVERGENiHBG080984.
InParanoidiO75808.
KOiK08582.
OMAiHGFQEHS.
OrthoDBiEOG7NKKJJ.
PhylomeDBiO75808.
TreeFamiTF322245.

Miscellaneous databases

GenomeRNAii6650.
NextBioi25919.
PROiO75808.
SOURCEiSearch...

Gene expression databases

BgeeiO75808.
CleanExiHS_SOLH.
ExpressionAtlasiO75808. baseline and differential.
GenevestigatoriO75808.

Family and domain databases

InterProiIPR022684. Calpain_cysteine_protease.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
IPR001876. Znf_RanBP2.
[Graphical view]
PfamiPF00648. Peptidase_C2. 1 hit.
PF00641. zf-RanBP. 3 hits.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00230. CysPc. 1 hit.
SM00547. ZnF_RBZ. 5 hits.
[Graphical view]
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
PS01358. ZF_RANBP2_1. 5 hits.
PS50199. ZF_RANBP2_2. 4 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "SOLH, a human homologue of the Drosophila melanogaster small optic lobes gene is a member of the calpain and zinc-finger gene families and maps to human chromosome 16p13.3 near CATM (cataract with microphthalmia)."
    Kamei M., Webb G.C., Young I.G., Campbell H.D.
    Genomics 51:197-206(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
    Tissue: Hippocampus.
  2. "Sequence, structure and pathology of the fully annotated terminal 2 Mb of the short arm of human chromosome 16."
    Daniels R.J., Peden J.F., Lloyd C., Horsley S.W., Clark K., Tufarelli C., Kearney L., Buckle V.J., Doggett N.A., Flint J., Higgs D.R.
    Hum. Mol. Genet. 10:339-352(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The sequence and analysis of duplication-rich human chromosome 16."
    Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G., Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E., Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J.
    , Buckingham J.M., Callen D.F., Campbell C.S., Campbell M.L., Campbell E.W., Caoile C., Challacombe J.F., Chasteen L.A., Chertkov O., Chi H.C., Christensen M., Clark L.M., Cohn J.D., Denys M., Detter J.C., Dickson M., Dimitrijevic-Bussod M., Escobar J., Fawcett J.J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Goodwin L.A., Grady D.L., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E., Huang W., Israni S., Jett J., Jewett P.B., Kadner K., Kimball H., Kobayashi A., Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y., Lowry S., Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J., Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D., Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L., Rash S., Retterer J., Ricke D.O., Robinson D.L., Rodriguez A., Salamov A., Saunders E.H., Scott D., Shough T., Stallings R.L., Stalvey M., Sutherland R.D., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Torney D.C., Tran-Gyamfi M., Tsai M., Ulanovsky L.E., Ustaszewska A., Vo N., White P.S., Williams A.L., Wills P.L., Wu J.-R., Wu K., Yang J., DeJong P., Bruce D., Doggett N.A., Deaven L., Schmutz J., Grimwood J., Richardson P., Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M., Myers R.M., Rubin E.M., Pennacchio L.A.
    Nature 432:988-994(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 525-1086 (ISOFORM 1).
    Tissue: Embryonic stem cell, Lung and Placenta.
  6. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCAN15_HUMAN
AccessioniPrimary (citable) accession number: O75808
Secondary accession number(s): B1B1M4
, Q2KHS2, Q8WTY9, Q9BUW0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 19, 2007
Last sequence update: October 31, 1998
Last modified: February 3, 2015
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.